pLenti-hTERT-Neo
| Vector Name | pLenti-hTERT-Neo |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attccaccat gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact 2581 accgcgaggt gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc 2641 tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg 2701 tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga 2761 aggagctggt ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg 2821 ccttcggctt cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca 2881 gcgtgcgcag ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg 2941 ggctgctgct gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc 3001 tctttgtgct ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc 3061 tcggcgctgc cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg 3121 gatgcgaacg ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag 3181 ccccgggtgc gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc 3241 ccaggcgtgg cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc 3301 acccgggcag gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac 3361 ccgccgaaga agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat 3421 ccgtgggccg ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg 3481 acacgccttg tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca 3541 aggagcagct gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc 3601 ggaggctcgt ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca 3661 ggttgccccg cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg 3721 ggaaccacgc gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg 3781 cggtcacccc agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc 3841 ccgaggagga ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc 3901 cctggcaggt gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct 3961 ggggctccag gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg 4021 ggaagcatgc caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cgggactgcg 4081 cttggctgcg caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg 4141 aggagatcct ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca 4201 ggtctttctt ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga 4261 agagtgtctg gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc 4321 tgcgggagct gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga 4381 cgtccagact ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact 4441 acgtcgtggg agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg 4501 tgaaggcact gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg 4561 cctctgtgct gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc 4621 gggcccagga cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg 4681 acaccatccc ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca 4741 cgtactgcgt gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg 4801 ccttcaagag ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg 4861 ctcacctgca ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc 4921 tgaatgaggc cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg 4981 tgcgcatcag gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct 5041 ccacgctgct ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc 5101 ggcgggacgg gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca 5161 cccacgcgaa aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg 5221 tgaacttgcg gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg 5281 cttttgttca gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc 5341 ggaccctgga ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc 5401 tcaccttcaa ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct 5461 tgcggctgaa gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt 5521 gcaccaacat ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc 5581 agctcccatt tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg 5641 acacggcctc cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg 5701 ccaagggcgc cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat 5761 tcctgctcaa gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga 5821 cagcccagac gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg 5881 cagccaaccc ggcactgccc tcagacttca agaccatcct ggactgatgg gacgcggccg 5941 ctctagaact agtggatccc ccgggctgca ggaattcact agtaccggta ggcctgtcga 6001 cgatatcggg cccgcggccg ctggatcctc tagataccca tacgacgtcc cagactacgc 6061 ttgatccccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6361 tattccagaa gtagtgagga ggcttttttg gaggcctagt gaccagatct aatgcgtgct 6421 agttttgcaa aaagcttggg ctgcaggtcg aggcggatct gatcaagaga caggatgagg 6481 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 6541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 6601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 6661 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 6721 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 6781 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 6841 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 6901 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 6961 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 7021 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 7081 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 7141 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 7201 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 7261 tcgccttctt gacgagttct tctgaacgcg ttccggaaat caacctctgg attacaaaat 7321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 7381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 7441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 7501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 7561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 7621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 7681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 7741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 7801 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg 7861 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga 7921 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 7981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 8041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 8101 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 8161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt 8341 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga 8401 atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8461 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9301 gattttggcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 9361 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 9421 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 9481 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 9541 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg 9601 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 9661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 9721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 9781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 9841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 9901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 9961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 10021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 10081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 10141 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 10201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 10261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 10321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 10381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 10441 ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 10501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 10561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 10621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 10681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 10741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 10801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 10861 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 10921 atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc 10981 gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt 11041 gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt 11101 gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg 11161 taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga 11221 ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta 11281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 11341 taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg 11401 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg 11461 ccgattggtg gaagtaaggt ggtacgat
FEATURES Location/Qualifiers source 1..11488 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" primer_bind 5942..5958 /note="SK primer" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" CDS 6035..6061 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 6261..6396 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 6491..7285 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 7298..7885 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(7769..7780) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 7895..8128 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 7948..8128 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 8200..8321 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(8650..9238) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 9650..10444 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attccaccat gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact 2581 accgcgaggt gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc 2641 tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg 2701 tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga 2761 aggagctggt ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg 2821 ccttcggctt cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca 2881 gcgtgcgcag ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg 2941 ggctgctgct gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc 3001 tctttgtgct ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc 3061 tcggcgctgc cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg 3121 gatgcgaacg ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag 3181 ccccgggtgc gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc 3241 ccaggcgtgg cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc 3301 acccgggcag gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac 3361 ccgccgaaga agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat 3421 ccgtgggccg ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg 3481 acacgccttg tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca 3541 aggagcagct gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc 3601 ggaggctcgt ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca 3661 ggttgccccg cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg 3721 ggaaccacgc gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg 3781 cggtcacccc agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc 3841 ccgaggagga ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc 3901 cctggcaggt gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct 3961 ggggctccag gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg 4021 ggaagcatgc caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cgggactgcg 4081 cttggctgcg caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg 4141 aggagatcct ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca 4201 ggtctttctt ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga 4261 agagtgtctg gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc 4321 tgcgggagct gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga 4381 cgtccagact ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact 4441 acgtcgtggg agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg 4501 tgaaggcact gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg 4561 cctctgtgct gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc 4621 gggcccagga cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg 4681 acaccatccc ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca 4741 cgtactgcgt gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg 4801 ccttcaagag ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg 4861 ctcacctgca ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc 4921 tgaatgaggc cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg 4981 tgcgcatcag gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct 5041 ccacgctgct ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc 5101 ggcgggacgg gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca 5161 cccacgcgaa aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg 5221 tgaacttgcg gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg 5281 cttttgttca gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc 5341 ggaccctgga ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc 5401 tcaccttcaa ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct 5461 tgcggctgaa gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt 5521 gcaccaacat ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc 5581 agctcccatt tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg 5641 acacggcctc cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg 5701 ccaagggcgc cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat 5761 tcctgctcaa gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga 5821 cagcccagac gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg 5881 cagccaaccc ggcactgccc tcagacttca agaccatcct ggactgatgg gacgcggccg 5941 ctctagaact agtggatccc ccgggctgca ggaattcact agtaccggta ggcctgtcga 6001 cgatatcggg cccgcggccg ctggatcctc tagataccca tacgacgtcc cagactacgc 6061 ttgatccccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6361 tattccagaa gtagtgagga ggcttttttg gaggcctagt gaccagatct aatgcgtgct 6421 agttttgcaa aaagcttggg ctgcaggtcg aggcggatct gatcaagaga caggatgagg 6481 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 6541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 6601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 6661 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 6721 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 6781 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 6841 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 6901 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 6961 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 7021 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 7081 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 7141 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 7201 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 7261 tcgccttctt gacgagttct tctgaacgcg ttccggaaat caacctctgg attacaaaat 7321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 7381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 7441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 7501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 7561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 7621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 7681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 7741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 7801 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg 7861 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga 7921 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 7981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 8041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 8101 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 8161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt 8341 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga 8401 atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8461 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9301 gattttggcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 9361 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 9421 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 9481 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 9541 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg 9601 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 9661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 9721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 9781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 9841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 9901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 9961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 10021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 10081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 10141 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 10201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 10261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 10321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 10381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 10441 ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 10501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 10561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 10621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 10681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 10741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 10801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 10861 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 10921 atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc 10981 gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt 11041 gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt 11101 gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg 11161 taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga 11221 ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta 11281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 11341 taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg 11401 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg 11461 ccgattggtg gaagtaaggt ggtacgat
FEATURES Location/Qualifiers source 1..11488 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" primer_bind 5942..5958 /note="SK primer" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" CDS 6035..6061 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 6261..6396 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 6491..7285 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 7298..7885 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(7769..7780) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 7895..8128 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 7948..8128 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 8200..8321 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(8650..9238) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 9650..10444 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"