pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro

Vector Name	pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
VectorType	Lentiviral Vector
Antibiotic Information	Bacterial: Ampicillin Mammalian: Puromycin
Sequencing Primers	U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3'
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg 
4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat 
4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt 
4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg 
4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg 
4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggcacggg 
4501 cagcttgccg ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa 
4561 cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag agctagctgc agtaacgcca 
4621 ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt 
4681 acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg 
4741 gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag 
4801 atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag 
4861 gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt 
4921 ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca 
4981 ctcggcgcgc cagtcctccg acagactgag tcgcccgggt accgcgatcg ccatggcccc 
5041 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat 
5101 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 
5161 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 
5221 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac 
5281 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 
5341 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 
5401 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 
5461 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 
5521 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 
5581 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 
5641 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 
5701 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 
5761 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 
5821 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 
5881 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 
5941 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 
6001 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 
6061 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 
6121 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc 
6181 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 
6241 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 
6301 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 
6361 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 
6421 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 
6481 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc 
6541 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 
6601 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt 
6661 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc 
6721 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 
6781 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 
6841 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 
6901 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 
6961 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 
7021 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 
7081 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 
7141 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 
7201 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 
7261 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 
7321 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 
7381 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 
7441 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 
7501 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 
7561 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct 
7621 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag 
7681 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct 
7741 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg 
7801 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 
7861 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga 
7921 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 
7981 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 
8041 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 
8101 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 
8161 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 
8221 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 
8281 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct 
8341 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 
8401 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga 
8461 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 
8521 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 
8581 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 
8641 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 
8701 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 
8761 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 
8821 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga 
8881 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 
8941 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga 
9001 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 
9061 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 
9121 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 
9181 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agacgcgtca 
9241 gtgtactaat tatgctctct tgaaattggc tggagatgtt gagagcaacc caggtcccat 
9301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 
9361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 
9421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 
9481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 
9541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 
9601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 
9661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 
9721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 
9781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 
9841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 
9901 ttaaacgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
9961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
10021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
10081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
10141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
10201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
10261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
10321 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
10381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
10441 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 
10501 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 
10561 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gagacaagat 
10621 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
10681 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
10741 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
10801 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
10861 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 
10921 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
10981 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 
11041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
11101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
11161 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc 
11221 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 
11281 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 
11341 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag 
11401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
11461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
11521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
11581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
11641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
11701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
11761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
11821 caaaatatta acgtttacaa tttcc

                            LOCUS dna 11845 bp 
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_2 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 2822..2827
/dbxref="REBASE:NruI"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 4910..7600
/sequence="ORF_3 rf(4)"
ORF 5033..9898
/sequence="ORF_1 rf(2)"
misc_binding 6436..6441
/dbxref="REBASE:ApaI"
Marker 9299..9898
/gene="puro marker"
misc_binding 10504..10509
/dbxref="REBASE:EcoRI"
Other Gene 10587..10638
/gene="delta_U3 other"
Other Gene 10639..10819
/gene="HIV-1_5_LTR other"
Rep_Origin 11031..11108
/gene="SV40 origin"
misc_binding 11111..11123
/dbxref="REBASE:SfiI"
Promoter 11199..11217
/gene="T7 prom"
Reporter 11225..11383
/gene="lacZ_a reporter"
Rep_Origin 11405..11711
/gene="f1 origin"

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 caggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac 60
GTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATG

amp prom(62,90)>>> 
|
61 attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa 120
TAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTT

ORF_2 rf(3)(132,992)>>> 
|
amp marker(132,992)>>> 
|
121 aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat 180
TTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTA

181 tttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatc 240
AAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAG

241 agttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgaga 300
TCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCT

301 gttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcg 360
CAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGC

361 cggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctc 420
GCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAG

421 agaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacag 480
TCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTC

481 taagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttc 540
ATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAG

541 tgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatg 600
ACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTAC

601 taactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtg 660
ATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCAC

661 acaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac 720
TGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATG

721 ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggac 780
AATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTG

781 cacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg 840
GTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCAC

841 agcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcg 900
TCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGC

901 tagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg 960
ATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGAC

961 agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatac 1020
TCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATG

1021 tttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttg 1080
AAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAAC

1081 ataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccg 1140
TATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGC

pBR322 origin(1147,1766)>>> 
|
1141 tagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgc 1200
ATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACG

1201 aaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactc 1260
TTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAG

1261 tttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgt 1320
AAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACA

1321 agccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgc 1380
TCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACG

1381 taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggact 1440
ATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGA

1441 caagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacac 1500
GTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTG

1501 agcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgag 1560
TCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTC

1561 aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcg 1620
TTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGC

1621 gaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctg 1680
CTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGAC

1681 tcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgga 1740
AGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCT

1741 gcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcctt 1800
CGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAA

1801 ttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcct 1860
AACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGA

1861 ttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcg 1920
AACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGC

1921 aggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt 1980
TCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAA

1981 aatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatta 2040
TTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAAT

lac prom(2075,2104)>>> 
|
2041 atgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgta 2100
TACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCAT

2101 tgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatt 2160
ACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAA

T3 prom(2174,2193)>>> 
|
2161 acgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagcttaat 2220
TGCGGTTCGCGCGTTAATTGGGAGTGATTTCCCTTGTTTTCGACCTCGACGTTCGAATTA

2221 gtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgc 2280
CATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACG

2281 cttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcg 2340
GAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGC

2341 tgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgcc 2400
ACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGG

HIV-1_5_LTR other(2448,2628)>>> 
|
2401 gcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggt 2460
CGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTTATTTGCCCAGAGAGACCA

2461 tagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctc 2520
ATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAG

2521 aataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggta 2580
TTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCAT

2581 actagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaa 2640
TGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTT

2641 cagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctga 2700
GTCCCTGGACTTTCGCTTTCCCTTTGGTCTCGAGAGAGCTGCGTCCTGAGCCGAACGACT

HIV-1_psi_pack other(2736,2780)>>> 
|
2701 agcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactag 2760
TCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATC

2761 cggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattag 2820
GCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATC

NruI 
|
2821 atcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaaca 2880
TAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGT

2881 tatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac 2940
ATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTG

2941 atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcaga 3000
TAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCT

3001 agaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggataga 3060
TCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCT

3061 gataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagac 3120
CTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTG

NotI 
|
3121 caccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaatt 3180
GTGGCGTGTCGTTCGCCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAA

3181 ggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccca 3240
CCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGT

RRE reg(3290,3523)>>> 
|
3241 ccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgt 3300
GGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACA

3301 tccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacgg 3360
AGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGGAGTTACTGCGACTGCC

3361 tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggcta 3420
ATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGAT

3421 ttgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaa 3480
AACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTT

3481 gaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgct 3540
CTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGA

3541 ctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctc 3600
GACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAG

3601 tggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacac 3660
ACCTTGTCTAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTG

3661 aagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaaga 3720
TTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCT

3721 attattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggct 3780
TAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGA

3781 gtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttt 3840
CACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAA

3841 tgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagac 3900
ACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTG

3901 ccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggaga 3960
GGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCT

HpaI 
|
3961 gagagacagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttt 4020
CTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCCAATTGAAA

4021 taaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagc 4080
ATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCATCTGTATTATCG

ClaI 
|
4081 aacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttATcga 4140
TTGTCTGTATGTTTGATTTCTTAATGTTTTTGTTTAATGTTTTTAAGTTTTAAAATAGCT

4141 tcacgagactagcCTCGAGCCCCAGTGGAAAGACGCGCAGGCAAAACGCACCACGTGACG 4200
AGTGCTCTGATCGGAGCTCGGGGTCACCTTTCTGCGCGTCCGTTTTGCGTGGTGCACTGC

hU6 prom(4229,4485)>>> 
|
4201 GAGCGTGACCGCGCGCCGAGCGCGCGCCAAGGTCGGGCAGGAAGAGGGCCTATTTCCCAT 4260
CTCGCACTGGCGCGCGGCTCGCGCGCGGTTCCAGCCCGTCCTTCTCCCGGATAAAGGGTA

4261 GATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTT 4320
CTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAA

4321 GACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGG 4380
CTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACC

NdeI 
|
4381 GTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTG 4440
CATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAAC

4441 AAAGTATTTCGATTTCTTGGGTTTATATATCTTGTGGAAAGGACGAAACACCGGCACGGG 4500
TTTCATAAAGCTAAAGAACCCAAATATATAGAACACCTTTCCTGCTTTGTGGCCGTGCCC

4501 CAGCTTGCCGGGttttagagctagaaatagcaagttaaaataaggctagtccgttatcaa 4560
GTCGAACGGCCCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTT

4561 cttgaaaaagtggcaccgagtcggtgcTTTTTTTCtcgagAGCTAGCTGCAGTAACGCCA 4620
GAACTTTTTCACCGTGGCTCAGCCACGAAAAAAAGAGCTCTCGATCGACGTCATTGCGGT

4621 TTTTGCAAGGCATGGAAAAATACCAAACCAAGAATAGAGAAGTTCAGATCAAGGGCGGGT 4680
AAAACGTTCCGTACCTTTTTATGGTTTGGTTCTTATCTCTTCAAGTCTAGTTCCCGCCCA

4681 ACATGAAAATAGCTAACGTTGGGCCAAACAGGATATCTGCGGTGAGCAGTTTCGGCCCCG 4740
TGTACTTTTATCGATTGCAACCCGGTTTGTCCTATAGACGCCACTCGTCAAAGCCGGGGC

4741 GCCCGGGGCCAAGAACAGATGGTCACCGCAGTTTCGGCCCCGGCCCGAGGCCAAGAACAG 4800
CGGGCCCCGGTTCTTGTCTACCAGTGGCGTCAAAGCCGGGGCCGGGCTCCGGTTCTTGTC

4801 ATGGTCCCCAGATATGGCCCAACCCTCAGCAGTTTCTTAAGACCCATCAGATGTTTCCAG 4860
TACCAGGGGTCTATACCGGGTTGGGAGTCGTCAAAGAATTCTGGGTAGTCTACAAAGGTC

ORF_3 rf(4)(4910,7600)<<< 
|
4861 GCTCCCCCAAGGACCTGAAATGACCCTGCGCCTTATTTGAATTAACCAATCAGCCTGCTT 4920
CGAGGGGGTTCCTGGACTTTACTGGGACGCGGAATAAACTTAATTGGTTAGTCGGACGAA

4921 CTCGCTTCTGTTCGCGCGCTTCTGCTTCCCGAGCTCTATAAAAGAGCTCACAACCCCTCA 4980
GAGCGAAGACAAGCGCGCGAAGACGAAGGGCTCGAGATATTTTCTCGAGTGTTGGGGAGT

ORF_1 rf(2)(5033,9898)>>> 
|
4981 CTCGGCGCGCCAGTCCTCCGACAGACTGAGTCGCCCGGGTACCGCGATCGCCATGGCCCC 5040
GAGCCGCGCGGTCAGGAGGCTGTCTGACTCAGCGGGCCCATGGCGCTAGCGGTACCGGGG

5041 AAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCAT 5100
TTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGCTGTTCTTCATGTCGTA

5101 CGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGT 5160
GCCGGACCTGTAGCCGTGGTTGAGACACCCGACCCGGCACTAGTGGCTGCTCATGTTCCA

5161 GCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCT 5220
CGGGTCGTTCTTTAAGTTCCACGACCCGTTGTGGCTGGCCGTGTCGTAGTTCTTCTTGGA

5221 GATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAAC 5280
CTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTCCGGTGGGCCGACTTCTCTTG

5281 CGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAG 5340
GCGGTCTTCTTCTATGTGGTCTGCCTTCTTGGCCTAGACGATAGACGTTCTCTAGAAGTC

5341 CAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGT 5400
GTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCTGACCTTCTCAGGAAGGACCA

5401 GGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGC 5460
CCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCGTTGTAGCACCTGCTCCACCG

5461 CTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGA 5520
GATGGTGCTCTTCATGGGGTGGTAGATGGTGGACTCTTTCTTTGACCACCTGTCGTGGCT

5521 CAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCA 5580
GTTCCGGCTGGACGCCGACTAGATAGACCGGGACCGGGTGTACTAGTTCAAGGCCCCGGT

5581 CTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCA 5640
GAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCGCTGCACCTGTTCGACAAGTAGGT

5641 GCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGA 5700
CGACCACGTCTGGATGTTGGTCGACAAGCTCCTTTTGGGGTAGTTGCGGTCGCCGCACCT

5701 CGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGC 5760
GCGGTTCCGGTAGGACAGACGGTCTGACTCGTTCTCGTCTGCCGACCTTTTAGACTAGCG

5761 CCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGcAACCTGATTGCCCTGAGCCTGGG 5820
GGTCGACGGGCCGCTCTTCTTCTTACCGGACAAGCCGTTGGACTAACGGGACTCGGACCC

5821 CCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAG 5880
GGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGGCTCCTACGGTTTGACGTCGACTC

5881 CAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGC 5940
GTTCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGGGTCTAGCCGCTGGTCATGCG

5941 CGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAG 6000
GCTGGACAAAGACCGGCGGTTCTTGGACAGGCTGCGGTAGGACGACTCGCTGTAGGACTC

6001 AGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGA 6060
TCACTTGTGGCTCTAGTGGTTCCGGGGGGACTCGCGGAGATACTAGTTCTCTATGCTGCT

6061 GCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTA 6120
CGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCCGTCGTCGACGGACTCTTCAT

6121 CAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGC 6180
GTTTCTCTAAAAGAAGCTGGTCTCGTTCTTGCCGATGCGGCCGATGTAACTGCCGCCTCG

6181 CAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGA 6240
GTCGGTCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTTTTCTACCTGCCGTGGCT

6241 GGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAA 6300
CCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCCTTCGTCGCCTGGAAGCTGTT

6301 CGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGA 6360
GCCGTCGTAGGGGGTGGTCTAGGTGGACCCTCTCGACGTGCGGTAAGACGCCGCCGTCCT

6361 AGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCG 6420
TCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAGCTCTTCTAGGACTGGAAGGC

ApaI 
|
6421 CATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAG 6480
GTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCGTCTAAGCGGACCTACTGGTC

6481 AAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTC 6540
TTTCTCGCTCCTTTGGTAGTGGGGGACCTTGAAGCTCCTTCACCACCTGTTCCCGCGAAG

6541 CGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGT 6600
GCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTCTTGGACGGGTTGCTCTTCCA

6601 GCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGT 6660
CGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCACATATTGCTCGACTGGTTTCA

6661 GAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGC 6720
CTTTATGCACTGGCTCCCTTACTCTTTCGGGCGGAAGGACTCGCCGCTCGTCTTTTTCCG

6721 CATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGA 6780
GTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTGGCACTTCGTCGACTTTCTCCT

6781 CTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTT 6840
GATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAGAGGCCGCACCTTCTAGCCAA

6841 CAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCT 6900
GTTGCGGAGGGACCCGTGTATGGTGCTAGACGACTTTTAATAGTTCCTGTTCCTGAAGGA

6901 GGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGA 6960
CCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTATAGCACGACTGGGACTGTGACAAACT

6961 GGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGT 7020
CCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGGGTGGACAAGCTGCTGTTTCA

7021 GATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGAT 7080
CTACTTCGTCGACTTCGCCGCCTCTATGTGGCCGACCCCGTCCGACTCGGCCTTCGACTA

7081 CAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGG 7140
GTTGCCGTAGGCCCTGTTCGTCAGGCCGTTCTGTTAGGACCTAAAGGACTTCAGGCTGCC

7141 CTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGA 7200
GAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTGTCGGACTGGAAATTTCTCCT

7201 CATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCT 7260
GTAGGTCTTTCGGGTCCACAGGCCGGTCCCGCTATCGGACGTGCTCGTGTAACGGTTAGA

7261 GGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCT 7320
CCGGCCGTCGGGGCGGTAATTCTTCCCGTAGGACGTCTGTCACTTCCACCACCTGCTCGA

7321 CGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAA 7380
GCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCACTAGCTTTACCGGTCTCTCTT

7381 CCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGG 7440
GGTCTGGTGGGTCTTCCCTGTCTTCTTGTCGGCGCTCTCTTACTTCGCCTAGCTTCTCCC

7441 CATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCA 7500
GTAGTTTCTCGACCCGTCGGTCTAGGACTTTCTTGTGGGGCACCTTTTGTGGGTCGACGT

7501 GAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGA 7560
CTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCCCTATACATGCACCTGGTCCT

7561 ACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCT 7620
TGACCTGTAGTTGGCCGACAGGCTGATGCTACACCTGGTATAGCACGGAGTCTCGAAAGA

7621 GAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAG 7680
CTTCCTGCTGAGGTAGCTGTTGTTCCACGACTGGTCTTCGCTGTTCTTGGCCCCGTTCTC

7681 CGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCT 7740
GCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTACTTCTTGATGACCGCCGTCGACGA

7741 GAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGG 7800
CTTGCGGTTCGACTAATGGGTCTCTTTCAAGCTGTTAGACTGGTTCCGGCTCTCTCCGCC

7801 CCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGAT 7860
GGACTCGCTTGACCTATTCCGGCCGAAGTAGTTCTCTGTCGACCACCTTTGGGCCGTCTA

7861 CACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGA 7920
GTGTTTCGTGCACCGTGTCTAGGACCTGAGGGCCTACTTGTGATTCATGCTGCTCTTACT

7921 CAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCG 7980
GTTCGACTAGGCCCTTCACTTTCACTAGTGGGACTTCAGGTTCGACCACAGGCTAAAGGC

7981 GAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGC 8040
CTTCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTGATGGTGGTGCGGGTGCTGCG

8041 CTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGA 8100
GATGGACTTGCGGCAGCACCCTTGGCGGGACTAGTTTTTCATGGGATTCGACCTTTCGCT

8101 GTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCA 8160
CAAGCACATGCCGCTGATGTTCCACATGCTGCACGCCTTCTACTAGCGGTTCTCGCTCGT

8161 GGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAA 8220
CCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCGTTGTAGTACTTGAAAAAGTT

8221 GACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGG 8280
CTGGCTCTAATGGGACCGGTTGCCGCTCTAGGCCTTCGCCGGAGACTAGCTCTGTTTGCC

8281 CGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCT 8340
GCTTTGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAACGGTGGCACGCCTTTCACGA

8341 GAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAA 8400
CTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCACGTCTGTCCGCCGAAGTCGTT

8401 AGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGA 8460
TCTCAGATAGGACGGGTTCTCCTTGTCGCTATTCGACTAGCGGTCTTTCTTCCTGACCCT

8461 CCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGC 8520
GGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGGATAAGACACGACCACCACCG

8521 CAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCAC 8580
GTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACACTTTCTCGACGACCCCTAGTG

8581 CATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTA 8640
GTAGTACCTTTCTTCGTCGAAGCTCTTCTTAGGGTAGCTGAAAGACCTTCGGTTCCCGAT

8641 CAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGA 8700
GTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTCATGAGGGACAAGCTCGACCT

8701 AAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGC 8760
TTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGACGTCTTCCCTTTGCTTGACCG

8761 CCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGG 8820
GGACGGGAGGTTTATACACTTGAAGGACATGGACCGGTCGGTGATACTCTTCGACTTCCC

8821 CTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGA 8880
GAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCTTGTCGTGTTCGTGATGGACCT

8881 CGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCT 8940
GCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCACTAGGACCGGCTGCGATTAGA

8941 GGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGA 9000
CCTGTTTCACGACAGGCGGATGTTGTTCGTGGCCCTATTCGGGTAGTCTCTCGTCCGGCT

9001 GAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTT 9060
CTTATAGTAGGTGGACAAATGGGACTGGTTAGACCCTCGGGGACGGCGGAAGTTCATGAA

9061 TGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCT 9120
ACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTTCTCCACGACCTGCGGTGGGA

9121 GATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGG 9180
CTAGGTGGTCTCGTAGTGGCCGGACATGCTCTGTGCCTAGCTGGACAGAGTCGACCCTCC

9181 CGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGACGCGTCA 9240
GCTGTTTTCCGGCCGCCGGTGCTTTTTCCGGCCGGTCCGTTTTTTCTTTTTCTGCGCAGT

puro marker(9299,9898)>>> 
|
9241 GTGTACTAATTATGCTCTCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTCCCAT 9300
CACATGATTAATACGAGAGAACTTTAACCGACCTCTACAACTCTCGTTGGGTCCAGGGTA

9301 GACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACG 9360
CTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGC

9361 CACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCG 9420
GTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGC

9421 CCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACAT 9480
GGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTA

9481 CGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAG 9540
GCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTC

9541 CGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTC 9600
GCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAG

9601 CCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCC 9660
GGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGG

9661 CGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAG 9720
GCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTC

9721 CGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGA 9780
GCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCT

9781 GACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGA 9840
CTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCT

9841 CGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGT 9900
GCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTCA

9901 TTAAACGtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattctt 9960
AATTTGCAGCTGTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAA

9961 aactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgct 10020
TTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGA

10021 attgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctt 10080
TAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAA

10081 tatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgac 10140
ATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTG

10141 gcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgct 10200
CGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGA

10201 ttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggaca 10260
AAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGT

10261 ggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtccttt 10320
CCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAA

10321 ccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtc 10380
GGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAG

10381 ccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcct 10440
GGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGA

10441 cttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgc 10500
GAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCG

EcoRI 
|
10501 ctggaattcgagctcggtacctttaagaccaatgacttacaaggcagctgtagatcttag 10560
GACCTTAAGCTCGAGCCATGGAAATTCTGGTTACTGAATGTTCCGTCGACATCTAGAATC

delta_U3 other(10587,10638)>>> 
|
10561 ccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgagacaagat 10620
GGTGAAAAATTTTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTCTGTTCTA

HIV-1_5_LTR other(10639,10819)>>> 
|
10621 ctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctct 10680
GACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGA

10681 ggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagta 10740
CCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCAT

10741 gtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtca 10800
CACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGT

10801 gtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataactt 10860
CACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAA

10861 gcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttac 10920
CGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATG

10921 aaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagt 10980
TTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCA

SV40 origin(11031,11108)>>> 
|
10981 tgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccc 11040
ACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGAGATCGATAGGGCGGGG

11041 taactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatg 11100
ATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATAC

SfiI 
|
11101 cagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttg 11160
GTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAAC

T7 prom(11199,11217)<<< 
|
11161 gaggcctaggcttttgcgtcgagacgtacccaattcgccctatagtgagtcgtattacgc 11220
CTCCGGATCCGAAAACGCAGCTCTGCATGGGTTAAGCGGGATATCACTCAGCATAATGCG

lacZ_a reporter(11225,11383)>>> 
|
11221 gcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaact 11280
CGCGAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGA

11281 taatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcac 11340
ATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTG

11341 cgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtag 11400
GCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATC

f1 origin(11405,11711)>>> 
|
11401 cggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccag 11460
GCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTC

11461 cgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctt 11520
GCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAA

11521 tccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggca 11580
AGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGT

11581 cctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgata 11640
GGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTAT

11641 gacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttcca 11700
CTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGT

11701 aactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgcc 11760
TTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGG

11761 gatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaa 11820
CTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATT

11821 caaaatattaacgtttacaatttcc 11845
GTTTTATAATTGCAAATGTTAAAGG