pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP

Vector Name	pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP
VectorType	Lentivector
Antibiotic Information	Bacterial: Kanamycin
Sequencing Primers	U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3'
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagggcct atttcccatg attccttcat
2461 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
2521 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
2581 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
2641 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtctaagaa ctttaaataa
2701 tttctactgt tgtagatttg tcttcctcga ggaagacaat ttttttagtc agtctagaga
2761 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
2821 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
2881 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
2941 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
3001 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
3061 ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
3121 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
3181 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
3241 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
3301 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
3361 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
3421 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
3481 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
3541 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
3601 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
3661 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
3721 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
3781 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
3841 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
3901 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgactg
3961 ctagcatgag catctaccag gagttcgtca acaagtattc actgagtaag acactgcggt
4021 tcgagctgat cccacagggc aagacactgg agaacatcaa ggcccgaggc ctgattctgg
4081 acgatgagaa gcgggcaaaa gactataaga aagccaagca gatcattgat aaataccacc
4141 agttctttat cgaggaaatt ctgagctccg tgtgcatcag tgaggatctg ctgcagaatt
4201 actcagacgt gtacttcaag ctgaagaaga gcgacgatga caacctgcag aaggacttca
4261 agtccgccaa ggacaccatc aagaaacaga ttagcgagta catcaaggac tccgaaaagt
4321 ttaaaaatct gttcaaccag aatctgatcg atgctaagaa aggccaggag tccgacctga
4381 tcctgtggct gaaacagtct aaggacaatg ggattgaact gttcaaggct aactccgata
4441 tcactgatat tgacgaggca ctggaaatca tcaagagctt caagggatgg accacatact
4501 ttaaaggctt ccacgagaac cgcaagaacg tgtactccag caacgacatt cctacctcca
4561 tcatctaccg aatcgtcgat gacaatctgc caaagttcct ggagaacaag gccaaatatg
4621 aatctctgaa ggacaaagct cccgaggcaa ttaattacga acagatcaag aaagatctgg
4681 ctgaggaact gacattcgat atcgactata agactagcga ggtgaaccag agggtctttt
4741 ccctggacga ggtgtttgaa atcgccaatt tcaacaatta cctgaaccag tccggcatta
4801 ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg ggagaatacc aagcgcaagg
4861 gaattaacga atacatcaat ctgtatagcc agcagatcaa cgacaaaact ctgaagaaat
4921 acaagatgtc tgtgctgttc aaacagatcc tgagtgatac cgagtccaag tcttttgtca
4981 ttgataaact ggaagatgac tcagacgtgg tcactaccat gcagagcttt tatgagcaga
5041 tcgccgcttt caagacagtg gaggaaaaat ctattaagga aactctgagt ctgctgttcg
5101 atgacctgaa agcccagaag ctggacctga gtaagatcta cttcaaaaac gataagagtc
5161 tgacagacct gtcacagcag gtgtttgatg actattccgt gattgggacc gccgtcctgg
5221 agtacattac acagcagatc gctccaaaga acctggataa tccctctaag aaagagcagg
5281 aactgatcgc taagaaaacc gagaaggcaa aatatctgag tctggaaaca attaagctgg
5341 cactggagga gttcaacaag cacagggata ttgacaaaca gtgccgcttt gaggaaatcc
5401 tggccaactt cgcagccatc cccatgattt ttgatgagat cgcccagaac aaagacaatc
5461 tggctcagat cagtattaag taccagaacc agggcaagaa agacctgctg caggcttcag
5521 cagaagatga cgtgaaagcc atcaaggatc tgctggacca gaccaacaat ctgctgcaca
5581 agctgaaaat cttccatatt agtcagtcag aggataaggc taatatcctg gataaagacg
5641 aacacttcta cctggtgttc gaggaatgtt acttcgagct ggcaaacatt gtccccctgt
5701 ataacaagat taggaactac atcacacaga agccttactc tgacgagaag tttaaactga
5761 acttcgaaaa tagtaccctg gccaacgggt gggataagaa caaggagcct gacaacacag
5821 ctatcctgtt catcaaggat gacaagtact atctgggagt gatgaataag aaaaacaata
5881 agatcttcga tgacaaagcc attaaggaga acaaagggga aggatacaag aaaatcgtgt
5941 ataagctgct gcccggcgca aataagatgc tgcctaaggt gttcttcagc gccaagagta
6001 tcaaattcta caacccatcc gaggacatcc tgcggattag aaatcactca acacatacta
6061 agaacgggag cccccagaag ggatatgaga aatttgagtt caacatcgag gattgcagga
6121 agtttattga cttctacaag cagagcatct ccaaacaccc tgaatggaag gattttggct
6181 tccggttttc cgacacacag agatataact ctatcgacga gttctaccgc gaggtggaaa
6241 atcaggggta taagctgact tttgagaaca tttctgaaag ttacatcgac agcgtggtca
6301 atcagggaaa gctgtacctg ttccagatct ataacaaaga tttttcagca tacagcaagg
6361 gcagaccaaa cctgcataca ctgtactgga aggccctgtt cgatgagagg aatctgcagg
6421 acgtggtcta taaactgaac ggagaggccg aactgtttta ccggaagcag tctattccta
6481 agaaaatcac tcacccagct aaggaggcca tcgctaacaa gaacaaggac aatcctaaga
6541 aagagagcgt gttcgaatac gatctgatta aggacaagcg gttcaccgaa gataagttct
6601 ttttccattg tccaatcacc attaacttca agtcaagcgg cgctaacaag ttcaacgacg
6661 agatcaatct gctgctgaag gaaaaagcaa acgatgtgca catcctgagc attgaccgag
6721 gagagcggca tctggcctac tataccctgg tggatggcaa agggaatatc attaagcagg
6781 atacattcaa catcattggc aatgaccgga tgaaaaccaa ctaccacgat aaactggctg
6841 caatcgagaa ggatagagac tcagctagga aggactggaa gaaaatcaac aacattaagg
6901 agatgaagga aggctatctg agccaggtgg tccatgagat tgcaaagctg gtcatcgaat
6961 acaatgccat tgtggtgttc gaggatctga acttcggctt taagaggggg cgctttaagg
7021 tggaaaaaca ggtctatcag aagctggaga aaatgctgat cgaaaagctg aattacctgg
7081 tgtttaaaga taacgagttc gacaagaccg gaggcgtcct gagagcctac cagctgacag
7141 ctccctttga aactttcaag aaaatgggaa aacagacagg catcatctac tatgtgccag
7201 ccggattcac ttccaagatc tgccccgtga ccggctttgt caaccagctg taccctaaat
7261 atgagtcagt gagcaagtcc caggaatttt tcagcaagtt cgataagatc tgttataatc
7321 tggacaaggg gtacttcgag ttttccttcg attacaagaa cttcggcgac aaggccgcta
7381 aggggaaatg gaccattgcc tccttcggat ctcgcctgat caactttcga aattccgata
7441 aaaaccacaa ttgggacact agggaggtgt acccaaccaa ggagctggaa aagctgctga
7501 aagactactc tatcgagtat ggacatggcg aatgcatcaa ggcagccatc tgtggcgaga
7561 gtgataagaa atttttcgcc aagctgacct cagtgctgaa tacaatcctg cagatgcgga
7621 actcaaagac cgggacagaa ctggactatc tgattagccc cgtggctgat gtcaacggaa
7681 acttcttcga cagcagacag gcacccaaaa atatgcctca ggatgcagac gccaacgggg
7741 cctaccacat cgggctgaag ggactgatgc tgctgggccg gatcaagaac aatcaggagg
7801 ggaagaagct gaacctggtc attaagaacg aggaatactt cgagtttgtc cagaatagaa
7861 ataacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct
7921 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat
7981 atgatgtccc cgactatgcc ccaaagaaga agcggaaggt ccagtgtact aattatgctc
8041 tcttgaaatt ggctggagat gttgagagca acccaggtcc cgaattcatg gtgagcaagg
8101 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
8161 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
8221 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
8281 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
8341 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
8401 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
8461 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
8521 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
8581 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
8641 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
8701 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
8761 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagtc gacacgcgtt
8821 ccggaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
8881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
8941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
9001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
9061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
9121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
9181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
9241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
9301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
9361 gtctcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg
9421 gatggaaggg ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc
9481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
9541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
9601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
9661 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
9721 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
9781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
9841 tgtatcttat catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat
9901 tatgggtatt atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca
9961 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
10021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
10081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
10141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
10201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
10261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
10321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
10381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
10441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
10501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
10561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
10621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
10681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
10741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
10801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
10861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
10921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
10981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
11041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
11101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
11161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
11221 taatagtttg cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa
11281 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
11341 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
11401 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
11461 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga
11521 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
11581 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
11641 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
11701 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
11761 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
11821 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
11881 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
11941 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
12001 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
12061 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
12121 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
12181 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
12241 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
12301 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
12361 ttctgaattt tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
12421 cggcaacatc ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt
12481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
12541 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag
12601 gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
12661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
12721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg
12781 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
12841 aatatacctt

                            LOCUS       dna                     12850 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     ORF             2749..3687
                     /sequence="ORF_4 rf(5)"
     misc_binding    2753..2758
                     /dbxref="REBASE:XbaI"
     Promoter        2770..3947
                     /gene="EF1a prom"
     misc_binding    3382..3387
                     /dbxref="REBASE:ApaI"
     ORF             3966..8807
                     /sequence="ORF_3 rf(3)"
     misc_binding    4347..4352
                     /dbxref="REBASE:ClaI"
     misc_binding    7914..7919
                     /dbxref="REBASE:BamHI"
     Tag             7920..7946
                     /gene="HA tag"
     misc_binding    8082..8087
                     /dbxref="REBASE:EcoRI"
     Reporter        8088..8804
                     /gene="EGFP reporter"
     Other Gene      9423..9475
                     /gene="delta_U3 other"
     Other Gene      9476..9656
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      10162..10781
                     /gene="pBR322 origin"
     Promoter        11434..11483
                     /gene="NEOKAN prom"
     ORF             11572..12366
                     /sequence="ORF_2 rf(1)"
     Marker          11575..12363
                     /gene="NTP_II marker"
     Rep_Origin      12471..12776
                     /gene="f1 origin"
BASE COUNT    3526 a   2989 c   3386 g   2949 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggagggcct atttcccatg attccttcat     
     2461 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca     
     2521 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag     
     2581 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg     
     2641 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtctaagaa ctttaaataa     
     2701 tttctactgt tgtagatttg tcttcctcga ggaagacaat ttttttagtc agtctagaga     
     2761 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag     
     2821 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac     
     2881 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat     
     2941 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag     
     3001 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg     
     3061 ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa     
     3121 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg     
     3181 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc     
     3241 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt     
     3301 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt     
     3361 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg     
     3421 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc     
     3481 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt     
     3541 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa     
     3601 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg     
     3661 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc     
     3721 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt     
     3781 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact     
     3841 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc     
     3901 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgactg     
     3961 ctagcatgag catctaccag gagttcgtca acaagtattc actgagtaag acactgcggt     
     4021 tcgagctgat cccacagggc aagacactgg agaacatcaa ggcccgaggc ctgattctgg     
     4081 acgatgagaa gcgggcaaaa gactataaga aagccaagca gatcattgat aaataccacc     
     4141 agttctttat cgaggaaatt ctgagctccg tgtgcatcag tgaggatctg ctgcagaatt     
     4201 actcagacgt gtacttcaag ctgaagaaga gcgacgatga caacctgcag aaggacttca     
     4261 agtccgccaa ggacaccatc aagaaacaga ttagcgagta catcaaggac tccgaaaagt     
     4321 ttaaaaatct gttcaaccag aatctgatcg atgctaagaa aggccaggag tccgacctga     
     4381 tcctgtggct gaaacagtct aaggacaatg ggattgaact gttcaaggct aactccgata     
     4441 tcactgatat tgacgaggca ctggaaatca tcaagagctt caagggatgg accacatact     
     4501 ttaaaggctt ccacgagaac cgcaagaacg tgtactccag caacgacatt cctacctcca     
     4561 tcatctaccg aatcgtcgat gacaatctgc caaagttcct ggagaacaag gccaaatatg     
     4621 aatctctgaa ggacaaagct cccgaggcaa ttaattacga acagatcaag aaagatctgg     
     4681 ctgaggaact gacattcgat atcgactata agactagcga ggtgaaccag agggtctttt     
     4741 ccctggacga ggtgtttgaa atcgccaatt tcaacaatta cctgaaccag tccggcatta     
     4801 ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg ggagaatacc aagcgcaagg     
     4861 gaattaacga atacatcaat ctgtatagcc agcagatcaa cgacaaaact ctgaagaaat     
     4921 acaagatgtc tgtgctgttc aaacagatcc tgagtgatac cgagtccaag tcttttgtca     
     4981 ttgataaact ggaagatgac tcagacgtgg tcactaccat gcagagcttt tatgagcaga     
     5041 tcgccgcttt caagacagtg gaggaaaaat ctattaagga aactctgagt ctgctgttcg     
     5101 atgacctgaa agcccagaag ctggacctga gtaagatcta cttcaaaaac gataagagtc     
     5161 tgacagacct gtcacagcag gtgtttgatg actattccgt gattgggacc gccgtcctgg     
     5221 agtacattac acagcagatc gctccaaaga acctggataa tccctctaag aaagagcagg     
     5281 aactgatcgc taagaaaacc gagaaggcaa aatatctgag tctggaaaca attaagctgg     
     5341 cactggagga gttcaacaag cacagggata ttgacaaaca gtgccgcttt gaggaaatcc     
     5401 tggccaactt cgcagccatc cccatgattt ttgatgagat cgcccagaac aaagacaatc     
     5461 tggctcagat cagtattaag taccagaacc agggcaagaa agacctgctg caggcttcag     
     5521 cagaagatga cgtgaaagcc atcaaggatc tgctggacca gaccaacaat ctgctgcaca     
     5581 agctgaaaat cttccatatt agtcagtcag aggataaggc taatatcctg gataaagacg     
     5641 aacacttcta cctggtgttc gaggaatgtt acttcgagct ggcaaacatt gtccccctgt     
     5701 ataacaagat taggaactac atcacacaga agccttactc tgacgagaag tttaaactga     
     5761 acttcgaaaa tagtaccctg gccaacgggt gggataagaa caaggagcct gacaacacag     
     5821 ctatcctgtt catcaaggat gacaagtact atctgggagt gatgaataag aaaaacaata     
     5881 agatcttcga tgacaaagcc attaaggaga acaaagggga aggatacaag aaaatcgtgt     
     5941 ataagctgct gcccggcgca aataagatgc tgcctaaggt gttcttcagc gccaagagta     
     6001 tcaaattcta caacccatcc gaggacatcc tgcggattag aaatcactca acacatacta     
     6061 agaacgggag cccccagaag ggatatgaga aatttgagtt caacatcgag gattgcagga     
     6121 agtttattga cttctacaag cagagcatct ccaaacaccc tgaatggaag gattttggct     
     6181 tccggttttc cgacacacag agatataact ctatcgacga gttctaccgc gaggtggaaa     
     6241 atcaggggta taagctgact tttgagaaca tttctgaaag ttacatcgac agcgtggtca     
     6301 atcagggaaa gctgtacctg ttccagatct ataacaaaga tttttcagca tacagcaagg     
     6361 gcagaccaaa cctgcataca ctgtactgga aggccctgtt cgatgagagg aatctgcagg     
     6421 acgtggtcta taaactgaac ggagaggccg aactgtttta ccggaagcag tctattccta     
     6481 agaaaatcac tcacccagct aaggaggcca tcgctaacaa gaacaaggac aatcctaaga     
     6541 aagagagcgt gttcgaatac gatctgatta aggacaagcg gttcaccgaa gataagttct     
     6601 ttttccattg tccaatcacc attaacttca agtcaagcgg cgctaacaag ttcaacgacg     
     6661 agatcaatct gctgctgaag gaaaaagcaa acgatgtgca catcctgagc attgaccgag     
     6721 gagagcggca tctggcctac tataccctgg tggatggcaa agggaatatc attaagcagg     
     6781 atacattcaa catcattggc aatgaccgga tgaaaaccaa ctaccacgat aaactggctg     
     6841 caatcgagaa ggatagagac tcagctagga aggactggaa gaaaatcaac aacattaagg     
     6901 agatgaagga aggctatctg agccaggtgg tccatgagat tgcaaagctg gtcatcgaat     
     6961 acaatgccat tgtggtgttc gaggatctga acttcggctt taagaggggg cgctttaagg     
     7021 tggaaaaaca ggtctatcag aagctggaga aaatgctgat cgaaaagctg aattacctgg     
     7081 tgtttaaaga taacgagttc gacaagaccg gaggcgtcct gagagcctac cagctgacag     
     7141 ctccctttga aactttcaag aaaatgggaa aacagacagg catcatctac tatgtgccag     
     7201 ccggattcac ttccaagatc tgccccgtga ccggctttgt caaccagctg taccctaaat     
     7261 atgagtcagt gagcaagtcc caggaatttt tcagcaagtt cgataagatc tgttataatc     
     7321 tggacaaggg gtacttcgag ttttccttcg attacaagaa cttcggcgac aaggccgcta     
     7381 aggggaaatg gaccattgcc tccttcggat ctcgcctgat caactttcga aattccgata     
     7441 aaaaccacaa ttgggacact agggaggtgt acccaaccaa ggagctggaa aagctgctga     
     7501 aagactactc tatcgagtat ggacatggcg aatgcatcaa ggcagccatc tgtggcgaga     
     7561 gtgataagaa atttttcgcc aagctgacct cagtgctgaa tacaatcctg cagatgcgga     
     7621 actcaaagac cgggacagaa ctggactatc tgattagccc cgtggctgat gtcaacggaa     
     7681 acttcttcga cagcagacag gcacccaaaa atatgcctca ggatgcagac gccaacgggg     
     7741 cctaccacat cgggctgaag ggactgatgc tgctgggccg gatcaagaac aatcaggagg     
     7801 ggaagaagct gaacctggtc attaagaacg aggaatactt cgagtttgtc cagaatagaa     
     7861 ataacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct     
     7921 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat     
     7981 atgatgtccc cgactatgcc ccaaagaaga agcggaaggt ccagtgtact aattatgctc     
     8041 tcttgaaatt ggctggagat gttgagagca acccaggtcc cgaattcatg gtgagcaagg     
     8101 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg     
     8161 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc     
     8221 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc     
     8281 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct     
     8341 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg     
     8401 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg     
     8461 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca     
     8521 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga     
     8581 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc     
     8641 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc     
     8701 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg     
     8761 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagtc gacacgcgtt     
     8821 ccggaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg     
     8881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt     
     8941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg     
     9001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc     
     9061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc     
     9121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc     
     9181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc     
     9241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg     
     9301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc     
     9361 gtctcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg     
     9421 gatggaaggg ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc     
     9481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct     
     9541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga     
     9601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag     
     9661 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag     
     9721 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa     
     9781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa     
     9841 tgtatcttat catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat     
     9901 tatgggtatt atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca     
     9961 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc     
    10021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     
    10081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     
    10141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     
    10201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     
    10261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     
    10321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     
    10381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     
    10441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     
    10501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     
    10561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     
    10621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     
    10681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     
    10741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     
    10801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     
    10861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     
    10921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     
    10981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     
    11041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     
    11101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     
    11161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     
    11221 taatagtttg cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa     
    11281 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca     
    11341 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag     
    11401 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct     
    11461 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga     
    11521 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa     
    11581 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     
    11641 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     
    11701 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag     
    11761 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     
    11821 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     
    11881 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     
    11941 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     
    12001 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     
    12061 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat     
    12121 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     
    12181 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     
    12241 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     
    12301 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     
    12361 ttctgaattt tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     
    12421 cggcaacatc ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt     
    12481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     
    12541 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag     
    12601 gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg     
    12661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc     
    12721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg     
    12781 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat     
    12841 aatatacctt

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1     ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
      AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61    gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
      CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

                                               Encap other(162,310)>>> 
                                               |
121   cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
      GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181   aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
      TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241   aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
      TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301   tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
      ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

          amp prom(365,393)<<< 
          |
361   ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
      CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

                                             HpaI 
                                             |
421   gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
      CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481   gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
      CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC

541   caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
      GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC

601   tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
      ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA

                                                            HIV-1_5_LTR other(715,895)>>> 
                                                            |
661   gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
      CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA

721   ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
      GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA

781   aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
      TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG

841   tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
      AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG

901   gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
      CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG

                                                   HIV-1_psi_pack other(1006,1050)>>> 
                                                   |
961   ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
      CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT

1021  ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
      AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC

                    NruI 
                    |
1081  ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
      CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT

1141  aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
      TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG

1201  tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
      ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT

1261  caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
      GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG

1321  aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
      TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT

1381  aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
      TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC

1441  ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
      CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT

                                                              RRE reg(1557,1790)>>> 
                                                              |
1501  gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
      CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT

                                             ORF_1 rf(1)(1600,2301)>>> 
                                             |
1561  gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
      CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC

1621  ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
      GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC

1681  agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
      TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG

1741  caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
      GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC

1801  ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
      CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA

1861  aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
      TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG

1921  aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
      TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA

1981  gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
      CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT

2041  aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
      TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT

2101  atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
      TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC

2161  tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
      AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT

2221  ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
      CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT

2281  aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
      TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC

2341  aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
      TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT

2401  aattcaaaattttcgggtttttcgaacctagggagggcctatttcccatgattccttcat 2460
      TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCCCGGATAAAGGGTACTAAGGAAGTA

2461  atttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaaca 2520
      TAAACGTATATGCTATGTTCCGACAATCTCTCTATTAACCTTAATTAAACTGACATTTGT

2521  caaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcag 2580
      GTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCATCAAACGTC

2581  ttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcg 2640
      AAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGC

2641  atttcttggctttatatatcttgtggaaaggacgaaacaccgtctaagaactttaaataa 2700
      TAAAGAACCGAAATATATAGAACACCTTTCCTGCTTTGTGGCAGATTCTTGAAATTTATT

                                                           XbaI 
                                                           |
                                                      ORF_4 rf(5)(2749,3687)<<< 
                                                      |    |
2701  tttctactgttgtagatttgtcttcctcgaggaagacaatttttttagtcagtctagaga 2760
      AAAGATGACAACATCTAAACAGAAGGAGCTCCTTCTGTTAAAAAAATCAGTCAGATCTCT

               EF1a prom(2770,3947)>>> 
               |
2761  gtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgag 2820
      CACCCTTAACCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTC

2821  aagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaac 2880
      TTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTG

2881  tgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtat 2940
      ACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATA

2941  ataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacag 3000
      TATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTC

3001  gtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtg 3060
      CATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCAC

3061  ccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaa 3120
      GGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTT

3121  gtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttg 3180
      CACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAAC

3181  aggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtc 3240
      TCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAG

3241  tcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgcttt 3300
      AGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAA

3301  ttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttt 3360
      AAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAA

                                ApaI 
                                |
3361  ttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggg 3420
      AACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCC

3421  gcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctc 3480
      CGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAG

3481  tggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggt 3540
      ACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCA

3541  cggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaa 3600
      GCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTT

3601  aatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaaggg 3660
      TTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCC

3661  cctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggc 3720
      GGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCG

3721  acctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggtttt 3780
      TGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAA

3781  atgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcact 3840
      TACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGA

3841  tgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagc 3900
      ACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCG

3901  ctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgaggaatttcgactg 3960
      GAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTCCTTAAAGCTGAC

           ORF_3 rf(3)(3966,8807)>>> 
           |
3961  ctagcatgagcatctaccaggagttcgtcaacaagtattcactgagtaagacactgcggt 4020
      GATCGTACTCGTAGATGGTCCTCAAGCAGTTGTTCATAAGTGACTCATTCTGTGACGCCA

4021  tcgagctgatcccacagggcaagacactggagaacatcaaggcccgaggcctgattctgg 4080
      AGCTCGACTAGGGTGTCCCGTTCTGTGACCTCTTGTAGTTCCGGGCTCCGGACTAAGACC

4081  acgatgagaagcgggcaaaagactataagaaagccaagcagatcattgataaataccacc 4140
      TGCTACTCTTCGCCCGTTTTCTGATATTCTTTCGGTTCGTCTAGTAACTATTTATGGTGG

4141  agttctttatcgaggaaattctgagctccgtgtgcatcagtgaggatctgctgcagaatt 4200
      TCAAGAAATAGCTCCTTTAAGACTCGAGGCACACGTAGTCACTCCTAGACGACGTCTTAA

4201  actcagacgtgtacttcaagctgaagaagagcgacgatgacaacctgcagaaggacttca 4260
      TGAGTCTGCACATGAAGTTCGACTTCTTCTCGCTGCTACTGTTGGACGTCTTCCTGAAGT

4261  agtccgccaaggacaccatcaagaaacagattagcgagtacatcaaggactccgaaaagt 4320
      TCAGGCGGTTCCTGTGGTAGTTCTTTGTCTAATCGCTCATGTAGTTCCTGAGGCTTTTCA

                                  ClaI 
                                  |
4321  ttaaaaatctgttcaaccagaatctgatcgatgctaagaaaggccaggagtccgacctga 4380
      AATTTTTAGACAAGTTGGTCTTAGACTAGCTACGATTCTTTCCGGTCCTCAGGCTGGACT

4381  tcctgtggctgaaacagtctaaggacaatgggattgaactgttcaaggctaactccgata 4440
      AGGACACCGACTTTGTCAGATTCCTGTTACCCTAACTTGACAAGTTCCGATTGAGGCTAT

4441  tcactgatattgacgaggcactggaaatcatcaagagcttcaagggatggaccacatact 4500
      AGTGACTATAACTGCTCCGTGACCTTTAGTAGTTCTCGAAGTTCCCTACCTGGTGTATGA

4501  ttaaaggcttccacgagaaccgcaagaacgtgtactccagcaacgacattcctacctcca 4560
      AATTTCCGAAGGTGCTCTTGGCGTTCTTGCACATGAGGTCGTTGCTGTAAGGATGGAGGT

4561  tcatctaccgaatcgtcgatgacaatctgccaaagttcctggagaacaaggccaaatatg 4620
      AGTAGATGGCTTAGCAGCTACTGTTAGACGGTTTCAAGGACCTCTTGTTCCGGTTTATAC

4621  aatctctgaaggacaaagctcccgaggcaattaattacgaacagatcaagaaagatctgg 4680
      TTAGAGACTTCCTGTTTCGAGGGCTCCGTTAATTAATGCTTGTCTAGTTCTTTCTAGACC

4681  ctgaggaactgacattcgatatcgactataagactagcgaggtgaaccagagggtctttt 4740
      GACTCCTTGACTGTAAGCTATAGCTGATATTCTGATCGCTCCACTTGGTCTCCCAGAAAA

4741  ccctggacgaggtgtttgaaatcgccaatttcaacaattacctgaaccagtccggcatta 4800
      GGGACCTGCTCCACAAACTTTAGCGGTTAAAGTTGTTAATGGACTTGGTCAGGCCGTAAT

4801  ctaaattcaataccatcattggcgggaagtttgtgaacggggagaataccaagcgcaagg 4860
      GATTTAAGTTATGGTAGTAACCGCCCTTCAAACACTTGCCCCTCTTATGGTTCGCGTTCC

4861  gaattaacgaatacatcaatctgtatagccagcagatcaacgacaaaactctgaagaaat 4920
      CTTAATTGCTTATGTAGTTAGACATATCGGTCGTCTAGTTGCTGTTTTGAGACTTCTTTA

4921  acaagatgtctgtgctgttcaaacagatcctgagtgataccgagtccaagtcttttgtca 4980
      TGTTCTACAGACACGACAAGTTTGTCTAGGACTCACTATGGCTCAGGTTCAGAAAACAGT

4981  ttgataaactggaagatgactcagacgtggtcactaccatgcagagcttttatgagcaga 5040
      AACTATTTGACCTTCTACTGAGTCTGCACCAGTGATGGTACGTCTCGAAAATACTCGTCT

5041  tcgccgctttcaagacagtggaggaaaaatctattaaggaaactctgagtctgctgttcg 5100
      AGCGGCGAAAGTTCTGTCACCTCCTTTTTAGATAATTCCTTTGAGACTCAGACGACAAGC

5101  atgacctgaaagcccagaagctggacctgagtaagatctacttcaaaaacgataagagtc 5160
      TACTGGACTTTCGGGTCTTCGACCTGGACTCATTCTAGATGAAGTTTTTGCTATTCTCAG

5161  tgacagacctgtcacagcaggtgtttgatgactattccgtgattgggaccgccgtcctgg 5220
      ACTGTCTGGACAGTGTCGTCCACAAACTACTGATAAGGCACTAACCCTGGCGGCAGGACC

5221  agtacattacacagcagatcgctccaaagaacctggataatccctctaagaaagagcagg 5280
      TCATGTAATGTGTCGTCTAGCGAGGTTTCTTGGACCTATTAGGGAGATTCTTTCTCGTCC

5281  aactgatcgctaagaaaaccgagaaggcaaaatatctgagtctggaaacaattaagctgg 5340
      TTGACTAGCGATTCTTTTGGCTCTTCCGTTTTATAGACTCAGACCTTTGTTAATTCGACC

5341  cactggaggagttcaacaagcacagggatattgacaaacagtgccgctttgaggaaatcc 5400
      GTGACCTCCTCAAGTTGTTCGTGTCCCTATAACTGTTTGTCACGGCGAAACTCCTTTAGG

5401  tggccaacttcgcagccatccccatgatttttgatgagatcgcccagaacaaagacaatc 5460
      ACCGGTTGAAGCGTCGGTAGGGGTACTAAAAACTACTCTAGCGGGTCTTGTTTCTGTTAG

5461  tggctcagatcagtattaagtaccagaaccagggcaagaaagacctgctgcaggcttcag 5520
      ACCGAGTCTAGTCATAATTCATGGTCTTGGTCCCGTTCTTTCTGGACGACGTCCGAAGTC

5521  cagaagatgacgtgaaagccatcaaggatctgctggaccagaccaacaatctgctgcaca 5580
      GTCTTCTACTGCACTTTCGGTAGTTCCTAGACGACCTGGTCTGGTTGTTAGACGACGTGT

5581  agctgaaaatcttccatattagtcagtcagaggataaggctaatatcctggataaagacg 5640
      TCGACTTTTAGAAGGTATAATCAGTCAGTCTCCTATTCCGATTATAGGACCTATTTCTGC

5641  aacacttctacctggtgttcgaggaatgttacttcgagctggcaaacattgtccccctgt 5700
      TTGTGAAGATGGACCACAAGCTCCTTACAATGAAGCTCGACCGTTTGTAACAGGGGGACA

5701  ataacaagattaggaactacatcacacagaagccttactctgacgagaagtttaaactga 5760
      TATTGTTCTAATCCTTGATGTAGTGTGTCTTCGGAATGAGACTGCTCTTCAAATTTGACT

5761  acttcgaaaatagtaccctggccaacgggtgggataagaacaaggagcctgacaacacag 5820
      TGAAGCTTTTATCATGGGACCGGTTGCCCACCCTATTCTTGTTCCTCGGACTGTTGTGTC

5821  ctatcctgttcatcaaggatgacaagtactatctgggagtgatgaataagaaaaacaata 5880
      GATAGGACAAGTAGTTCCTACTGTTCATGATAGACCCTCACTACTTATTCTTTTTGTTAT

5881  agatcttcgatgacaaagccattaaggagaacaaaggggaaggatacaagaaaatcgtgt 5940
      TCTAGAAGCTACTGTTTCGGTAATTCCTCTTGTTTCCCCTTCCTATGTTCTTTTAGCACA

5941  ataagctgctgcccggcgcaaataagatgctgcctaaggtgttcttcagcgccaagagta 6000
      TATTCGACGACGGGCCGCGTTTATTCTACGACGGATTCCACAAGAAGTCGCGGTTCTCAT

6001  tcaaattctacaacccatccgaggacatcctgcggattagaaatcactcaacacatacta 6060
      AGTTTAAGATGTTGGGTAGGCTCCTGTAGGACGCCTAATCTTTAGTGAGTTGTGTATGAT

6061  agaacgggagcccccagaagggatatgagaaatttgagttcaacatcgaggattgcagga 6120
      TCTTGCCCTCGGGGGTCTTCCCTATACTCTTTAAACTCAAGTTGTAGCTCCTAACGTCCT

6121  agtttattgacttctacaagcagagcatctccaaacaccctgaatggaaggattttggct 6180
      TCAAATAACTGAAGATGTTCGTCTCGTAGAGGTTTGTGGGACTTACCTTCCTAAAACCGA

6181  tccggttttccgacacacagagatataactctatcgacgagttctaccgcgaggtggaaa 6240
      AGGCCAAAAGGCTGTGTGTCTCTATATTGAGATAGCTGCTCAAGATGGCGCTCCACCTTT

6241  atcaggggtataagctgacttttgagaacatttctgaaagttacatcgacagcgtggtca 6300
      TAGTCCCCATATTCGACTGAAAACTCTTGTAAAGACTTTCAATGTAGCTGTCGCACCAGT

6301  atcagggaaagctgtacctgttccagatctataacaaagatttttcagcatacagcaagg 6360
      TAGTCCCTTTCGACATGGACAAGGTCTAGATATTGTTTCTAAAAAGTCGTATGTCGTTCC

6361  gcagaccaaacctgcatacactgtactggaaggccctgttcgatgagaggaatctgcagg 6420
      CGTCTGGTTTGGACGTATGTGACATGACCTTCCGGGACAAGCTACTCTCCTTAGACGTCC

6421  acgtggtctataaactgaacggagaggccgaactgttttaccggaagcagtctattccta 6480
      TGCACCAGATATTTGACTTGCCTCTCCGGCTTGACAAAATGGCCTTCGTCAGATAAGGAT

6481  agaaaatcactcacccagctaaggaggccatcgctaacaagaacaaggacaatcctaaga 6540
      TCTTTTAGTGAGTGGGTCGATTCCTCCGGTAGCGATTGTTCTTGTTCCTGTTAGGATTCT

6541  aagagagcgtgttcgaatacgatctgattaaggacaagcggttcaccgaagataagttct 6600
      TTCTCTCGCACAAGCTTATGCTAGACTAATTCCTGTTCGCCAAGTGGCTTCTATTCAAGA

6601  ttttccattgtccaatcaccattaacttcaagtcaagcggcgctaacaagttcaacgacg 6660
      AAAAGGTAACAGGTTAGTGGTAATTGAAGTTCAGTTCGCCGCGATTGTTCAAGTTGCTGC

6661  agatcaatctgctgctgaaggaaaaagcaaacgatgtgcacatcctgagcattgaccgag 6720
      TCTAGTTAGACGACGACTTCCTTTTTCGTTTGCTACACGTGTAGGACTCGTAACTGGCTC

6721  gagagcggcatctggcctactataccctggtggatggcaaagggaatatcattaagcagg 6780
      CTCTCGCCGTAGACCGGATGATATGGGACCACCTACCGTTTCCCTTATAGTAATTCGTCC

6781  atacattcaacatcattggcaatgaccggatgaaaaccaactaccacgataaactggctg 6840
      TATGTAAGTTGTAGTAACCGTTACTGGCCTACTTTTGGTTGATGGTGCTATTTGACCGAC

6841  caatcgagaaggatagagactcagctaggaaggactggaagaaaatcaacaacattaagg 6900
      GTTAGCTCTTCCTATCTCTGAGTCGATCCTTCCTGACCTTCTTTTAGTTGTTGTAATTCC

6901  agatgaaggaaggctatctgagccaggtggtccatgagattgcaaagctggtcatcgaat 6960
      TCTACTTCCTTCCGATAGACTCGGTCCACCAGGTACTCTAACGTTTCGACCAGTAGCTTA

6961  acaatgccattgtggtgttcgaggatctgaacttcggctttaagagggggcgctttaagg 7020
      TGTTACGGTAACACCACAAGCTCCTAGACTTGAAGCCGAAATTCTCCCCCGCGAAATTCC

7021  tggaaaaacaggtctatcagaagctggagaaaatgctgatcgaaaagctgaattacctgg 7080
      ACCTTTTTGTCCAGATAGTCTTCGACCTCTTTTACGACTAGCTTTTCGACTTAATGGACC

7081  tgtttaaagataacgagttcgacaagaccggaggcgtcctgagagcctaccagctgacag 7140
      ACAAATTTCTATTGCTCAAGCTGTTCTGGCCTCCGCAGGACTCTCGGATGGTCGACTGTC

7141  ctccctttgaaactttcaagaaaatgggaaaacagacaggcatcatctactatgtgccag 7200
      GAGGGAAACTTTGAAAGTTCTTTTACCCTTTTGTCTGTCCGTAGTAGATGATACACGGTC

7201  ccggattcacttccaagatctgccccgtgaccggctttgtcaaccagctgtaccctaaat 7260
      GGCCTAAGTGAAGGTTCTAGACGGGGCACTGGCCGAAACAGTTGGTCGACATGGGATTTA

7261  atgagtcagtgagcaagtcccaggaatttttcagcaagttcgataagatctgttataatc 7320
      TACTCAGTCACTCGTTCAGGGTCCTTAAAAAGTCGTTCAAGCTATTCTAGACAATATTAG

7321  tggacaaggggtacttcgagttttccttcgattacaagaacttcggcgacaaggccgcta 7380
      ACCTGTTCCCCATGAAGCTCAAAAGGAAGCTAATGTTCTTGAAGCCGCTGTTCCGGCGAT

7381  aggggaaatggaccattgcctccttcggatctcgcctgatcaactttcgaaattccgata 7440
      TCCCCTTTACCTGGTAACGGAGGAAGCCTAGAGCGGACTAGTTGAAAGCTTTAAGGCTAT

7441  aaaaccacaattgggacactagggaggtgtacccaaccaaggagctggaaaagctgctga 7500
      TTTTGGTGTTAACCCTGTGATCCCTCCACATGGGTTGGTTCCTCGACCTTTTCGACGACT

7501  aagactactctatcgagtatggacatggcgaatgcatcaaggcagccatctgtggcgaga 7560
      TTCTGATGAGATAGCTCATACCTGTACCGCTTACGTAGTTCCGTCGGTAGACACCGCTCT

7561  gtgataagaaatttttcgccaagctgacctcagtgctgaatacaatcctgcagatgcgga 7620
      CACTATTCTTTAAAAAGCGGTTCGACTGGAGTCACGACTTATGTTAGGACGTCTACGCCT

7621  actcaaagaccgggacagaactggactatctgattagccccgtggctgatgtcaacggaa 7680
      TGAGTTTCTGGCCCTGTCTTGACCTGATAGACTAATCGGGGCACCGACTACAGTTGCCTT

7681  acttcttcgacagcagacaggcacccaaaaatatgcctcaggatgcagacgccaacgggg 7740
      TGAAGAAGCTGTCGTCTGTCCGTGGGTTTTTATACGGAGTCCTACGTCTGCGGTTGCCCC

7741  cctaccacatcgggctgaagggactgatgctgctgggccggatcaagaacaatcaggagg 7800
      GGATGGTGTAGCCCGACTTCCCTGACTACGACGACCCGGCCTAGTTCTTGTTAGTCCTCC

7801  ggaagaagctgaacctggtcattaagaacgaggaatacttcgagtttgtccagaatagaa 7860
      CCTTCTTCGACTTGGACCAGTAATTCTTGCTCCTTATGAAGCTCAAACAGGTCTTATCTT

                                                            BamHI 
                                                            |
7861  ataacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagggatcct 7920
      TATTGTTTTCCGGCCGCCGGTGCTTTTTCCGGCCGGTCCGTTTTTTCTTTTTCCCTAGGA

     HA tag(7920,7946)<<< 
     |
7921  acccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccat 7980
      TGGGTATGCTACAAGGTCTAATGCGAATAGGGATGCTGCACGGACTAATACGTATGGGTA

7981  atgatgtccccgactatgccccaaagaagaagcggaaggtcCAGTGTACTAATTATGCTC 8040
      TACTACAGGGGCTGATACGGGGTTTCTTCTTCGCCTTCCAGGTCACATGATTAATACGAG

                                                     EGFP reporter(8088,8804)>>> 
                                                     |
                                                EcoRI 
                                                |    |
8041  TCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTCCCgaattcatggtgagcaagg 8100
      AGAACTTTAACCGACCTCTACAACTCTCGTTGGGTCCAGGGCTTAAGTACCACTCGTTCC

8101  gcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacg 8160
      CGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGC

8161  gccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccc 8220
      CGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGG

8221  tgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccc 8280
      ACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGG

8281  tgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttct 8340
      ACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGA

8341  tcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacg 8400
      AGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGC

8401  gcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcg 8460
      CGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGC

8461  agctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtaca 8520
      TCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGT

8521  actacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga 8580
      TGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACT

8581  acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc 8640
      TGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCG

8641  agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccc 8700
      TCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGG

8701  agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcg 8760
      TCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGC

8761  tgaccgccgccgggatcactctcggcatggacgagctgtacaagtaagtcgacacgcgtt 8820
      ACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTCAGCTGTGCGCAA

8821  ccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatg 8880
      GGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATAC

8881  ttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgctt 8940
      AACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAA

8941  cccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgagg 9000
      GGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCC

9001  agttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaaccc 9060
      TCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGG

9061  ccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccc 9120
      GGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGG

9121  tccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctc 9180
      AGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAG

9181  ggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggc 9240
      CCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCG

9241  tgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcgg 9300
      ACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCC

9301  ccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgc 9360
      GGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCG

9361  gtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccg 9420
      CAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGC

        delta_U3 other(9423,9475)>>>                         HIV-1_5_LTR other(9476,9656)>>> 
        |                                                    |
9421  gatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtc 9480
      CTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAG

9481  tctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgct 9540
      AGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGA

9541  taagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtga 9600
      ATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACT

9601  ctctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtag 9660
      GAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATC

9661  tagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagag 9720
      ATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTC

9721  tgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaa 9780
      ACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTT

9781  tttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaa 9840
      AAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTT

9841  tgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcat 9900
      ACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTA

9901  tatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgca 9960
      ATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGT

9961  cagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactc 10020
      GTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAG

10021 gctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacg 10080
      CGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGC

10081 gttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa 10140
      CAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTT

                           pBR322 origin(10162,10781)<<< 
                           |
10141 ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctga 10200
      CCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACT

10201 cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaag 10260
      GCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTC

10261 ataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgct 10320
      TATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGA

10321 taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacg 10380
      ATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGC

10381 ctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacc 10440
      GACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGG

10441 ccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggt 10500
      GGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCA

10501 aagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggta 10560
      TTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCAT

10561 tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggac 10620
      ACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTG

10621 agtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctc 10680
      TCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAG

10681 ttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagat 10740
      AACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTA

10741 tacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgc 10800
      ATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCG

10801 tcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatctt 10860
      AGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAA

10861 cacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagta 10920
      GTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCAT

10921 aacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtct 10980
      TTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGA

10981 atttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggaggg 11040
      TAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCC

11041 cttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccaga 11100
      GAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCT

11101 tttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt 11160
      AAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAA

11161 atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagt 11220
      TAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCA

11221 taatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaa 11280
      ATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTT

11281 gccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggaca 11340
      CGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGT

11341 agggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatag 11400
      TCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATC

                                       NEOKAN prom(11434,11483)>>> 
                                       |
11401 ctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctct 11460
      GATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGA

11461 ggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctga 11520
      CCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACT

                                                            NTP_II marker(11575,12363)>>> 
                                                            |
                                                         ORF_2 rf(1)(11572,12366)>>> 
                                                         |  |
11521 tggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaa 11580
      ACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTT

11581 caagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgac 11640
      GTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTG

11641 tgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcagggg 11700
      ACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCC

11701 cgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgag 11760
      GCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTC

11761 gcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgtt 11820
      CGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAA

11821 gtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctg 11880
      CAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGAC

11881 tcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctg 11940
      AGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGAC

11941 catacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcga 12000
      GTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCT

12001 gcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcag 12060
      CGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTC

12061 gggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggat 12120
      CCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTA

12121 ctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttt 12180
      GAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAA

12181 tctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttg 12240
      AGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAAC

12241 gctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctt 12300
      CGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAA

12301 tacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttc 12360
      ATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAG

12361 ttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaat 12420
      AAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTA

                                                        f1 origin(12471,12776)<<< 
                                                        |
12421 cggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagt 12480
      GCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCA

12481 ttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgt 12540
      AACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCA

12541 ctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgag 12600
      GATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTC

12601 gtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgggg 12660
      CACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCC

12661 aaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggc 12720
      TTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCG

12721 gctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccg 12780
      CGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGC

12781 ctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaat 12840
      GATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTA

12841 aatatacctt 12850
      TTATATGGAA