pLenti-SV40-T-tsA58-No-Selection
| Vector Name | pLenti-SV40-T-tsA58-No-Selection |
| VectorType | Lentiviral plasmid |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression is driven by the CMV promoter, without any Tag or reporter. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg 50cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 100atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg 150acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg 200ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc 250cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag 350catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 400agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 450cctgacgtta actataacgg tcctaaggta gcgaaaatgt agtcttatgc 500aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 550tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 600tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg 650acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag 700ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag 750ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 800ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 850agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 900gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga 950cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg 1000actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 1050atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 1100gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 1150tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1200tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca 1250tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc 1300aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 1350ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1400caagcccgct gatcttcaga cctggaggag gagatatgag ggacattgga 1450gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1500gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt 1550gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta 1600tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct 1650ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca 1700gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 1750tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1800ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag 1850ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg 1900agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt 1950gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt 2000agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 2050ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2100atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc 2150accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 2200ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt 2250cgattagtga acggatctcg acggtatcga aagcttggga ttcgaattta 2300aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 2350ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2400aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact 2450tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 2500cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 2550tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2600tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 2650aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2700acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg 2750ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 2800tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 2850atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 2900atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt 2950agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3000atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc 3050agtgatagag agctagcccc gggatcgatc aattgagtac ttacgtaggt 3100accccagtgt ggtggcctgc aggtgaattc agccaccatg gataaagttt 3150taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg 3200agtgcctggg ggaatattcc tctgatgaga aaggcatatt taaaaaaatg 3250caaggagttt catcctgata aaggaggaga tgaagaaaaa atgaagaaaa 3300tgaatactct gtacaagaaa atggaagatg gagtaaaata tgctcatcaa 3350cctgactttg gaggcttctg ggatgcaact gagattccaa cctatggaac 3400tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt 3450gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa 3500cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 3550tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa 3600ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta 3650tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa 3700cagttataat cataacatac tgttttttct tactccacac aggcatagag 3750tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta 3800atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 3850agatccattt tctgttattg aggaaagttt gccaggtggg ttaaaggagc 3900atgattttaa tccagaagaa gcagaggaaa ctaaacaagt gtcctggaag 3950cttgtaacag agtatgcaat ggaaacaaaa tgtgatgatg tgttgttatt 4000gcttgggatg tacttggaat ttcagtacag ttttgaaatg tgtttaaaat 4050gtattaaaaa agaacagccc agccactata agtaccatga aaagcattat 4100gcaaatgctg ctatatttgc tgacagcaaa aaccaaaaaa ccatatgcca 4150acaggctgtt gatactgttt tagctaaaaa gcgggttgat agcctacaat 4200taactagaga acaaatgtta acaaacagat ttaatgatct tttggatagg 4250atggatataa tgtttggttc tacaggctct gctgacatag aagaatggat 4300ggctggagtt gcttggctac actgtttgtt gcccaaaatg gattcagtgg 4350tgtatgactt tttaaaatgc atggtgtaca acattcctaa aaaaagatac 4400tggctgttta aaggaccaat tgatagtggt aaaactacat tagcagctgt 4450tttgcttgaa ttatgtgggg ggaaagcttt aaatgttaat ttgcccttgg 4500acaggctgaa ctttgagcta ggagtagcta ttgaccagtt tttagtagtt 4550tttgaggatg taaagggcac tggaggggag tccagagatt tgccttcagg 4600tcagggaatt aataacctgg acaatttaag ggattatttg gatggcagtg 4650ttaaggtaaa cttagaaaag aaacacctaa ataaaagaac tcaaatattt 4700ccccctggaa tagtcaccat gaatgagtac agtgtgccta aaacactgca 4750ggccagattt gtaaaacaaa tagattttag gcccaaagat tatttaaagc 4800attgcctgga acgcagtgag tttttgttag aaaagagaat aattcaaagt 4850ggcattgctt tgcttcttat gttaatttgg tacagacctg tggctgagtt 4900tgctcaaagt attcagagca gaattgtgga gtggaaagag agattggaca 4950aagagtttag tttgtcagtg tatcaaaaaa tgaagtttaa tgtggctatg 5000ggaattggag ttttagattg gctaagaaac agtgatgatg atgatgaaga 5050cagccaggaa aatgctgata aaaatgaaga tggtggggag aagaacatgg 5100aagactcagg gcatgaaaca ggcattgatt cacagtccca aggctcattt 5150caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca 5200catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 5250aacctgaaac ataaaatgaa tgctcgagta cccatacgac gtcccagact 5300acgcttgagt ttaaacacgc gttccggaaa tcaacctctg gattacaaaa 5350tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5400tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5450ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5500aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5550gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 5600ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5650ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5700aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 5750ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5800cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5850cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg gatctccctt 5900tgggccgcct ccccgcctgt ccggatggaa gggctaattc actcccaacg 5950aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 6000tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6050caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6100tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6150tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6200gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6250taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 6300ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6350tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg 6400cattatgggt attatgggtc tgcattaatg aatcggccaa cgatcccggt 6450gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 6500tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6550agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6600gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6650aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6700tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6750caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6800tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6850ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6900cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6950cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 7000ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7050gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 7100aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7150gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7200ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 7250agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7300cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7350caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 7400tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7450aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7500ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7550tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7600agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7650gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 7700gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgaaaa 7750aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg 7800gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa 7850acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga 7900tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc 7950tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 8000ctttctcgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa 8050gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 8100aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 8150aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 8200gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 8250actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 8300cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 8350ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 8400tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 8450ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 8500cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 8550cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 8600cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 8650ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 8700tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 8750gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 8800ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 8850tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca 8900ttttttaacc aataggccga aatcggcaac atcccttata aatcaaaaga 8950atagaccgcg atagggttga gtgttgttcc agtttggaac aagagtccac 9000tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag 9050ggcgatggcc cactacgtga accatcaccc aaatcaagtt ttttgcggtc 9100gaggtgccgt aaagctctaa atcggaaccc taaagggagc ccccgattta 9150gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 9200gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 9250cgtaaccacc acacccgcgc gcttaatgcg ccgctacagg gcgcgtccat 9300tcgccattca ggatcgaatt aattcttaat taacatcatc aataatatac 9350ctt 9353