pLenti-Ras V12

Vector Name	pLenti-Ras V12
VectorType	Lentiviral Vector
Antibiotic Information	No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds.
Sequencing Primers	CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgt
agtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttgg
tgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt
aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaat
acggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt
agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta
gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagcc
agtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttg
accgacaattaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa
ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacggg
tctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacg
ggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc
ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtc
agtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcag
gactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag
gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggtta
aggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcc
tggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac
ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagcttta
gacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcgccgctgatcttcagacctggaggaggaga
tatgagggacaattgagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc
aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaa
gcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaat
ttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct
ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctg
tgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaa
attaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt
ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatga
tagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacca
ttatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga
cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagttccgcgttacataacttacgg
taaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcca
atagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat
gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggact
ttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgt
ggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca
acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctata
taagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgg
ctagcgtttaaacttaagcttggtaccggatccactagtgaattcatgacggaatataagctggtggtggtgggcgccgt
cggtgtgggcaagagtgcgctgaccatccagctgatccagaaccattttgtggacgaatacgaccccactatagaggatt
cctaccggaagcaggtggtcattgatggggagacgtgcctgttggacatcctggataccgccggccaggaggagtacagc
gccatgcgggaccagtacatgcgcaccggggagggcttcctgtgtgtgtttgccatcaacaacaccaagtcttttgagga
catccaccagtacagggagcagatcaaacgggtgaaggactcggatgacgtgcccatggtgctggtggggaacaagtgtg
acctggctgcacgcactgtggaatctcggcaggctcaggacctcgcccgaagctacggcatcccctacatcgagacctcg
gccaagacccggcagggagtggaggatgccttctacacgttggtgcgtgagatccggcagcacaagctgcggaagctgaa
ccctcctgatgagagtggccccggctgcatgagctgcaagtgtgtgctctcctgacgctcgagcatcatcaccatcacca
ttgatgatccggctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaag
catgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatc
tcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccg
ccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgagg
aggcttttttggaggcctagtgaccagatctaatgcgttctaatgcgtaccagatctaatgcgtgctagcgaccagatct
aatgcgtgctaggcccttttgcaaaaagcttgggctgcaggtcgaggcggatctgatcaagagacaggatgaggatcgtt
tcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcac
aacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgac
ctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagc
tgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctc
accttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgccca
ttcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctgga
cgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcg
tgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctg
ggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccg
cttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgag
cgggactctggggttcgatatctagcgaccagatctaattgcgtgcaaggcagctgtagatcttagccactttttaaaag
aaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggtta
gaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgct
tcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatct
ctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaac
ttgggcctgaccagatctaatgcgtaggccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatct
gttgttgcccgggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcct
aataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaag
ggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcag
atcgatctgcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaat
cggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgc
tcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaca
gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgct
ggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacag
gactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatac
ctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgt
tcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagt
ccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggt
gctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagcc
agttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgca
agcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaac
gaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaag
ttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcag
cgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatct
ggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaag
ggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagta
gttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgc
agaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcag
gtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctgg
ggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggg
gatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggcc
gcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtc
agcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggc
tatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgcta
ttgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaat
gcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactc
ggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccagg
ctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaa
tggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtg
atattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgc
atcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaat
aggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaag
agtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaacc
atcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagag
cttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagt
gtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcagga
tcgaattaattcttaattaacatcatcaataatatacctt

                            LOCUS dna 9169 bp 
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_3 rf(2)"
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
misc_binding 3062..3067
/dbxref="REBASE:KpnI"
misc_binding 3068..3073
/dbxref="REBASE:BamHI"
misc_binding 3080..3085
/dbxref="REBASE:EcoRI"
other_gene 3085..4146
/gene="RasV12"
Regulatory_Seq 3274..3843
/gene="Ras reg"
misc_binding 4078..4083
/dbxref="REBASE:ApaI"
misc_binding 4147..4152
/dbxref="REBASE:XhoI"
Tag 4153..4170
/gene="6xHis tag"
Regulatory_Seq 4181..4417
/gene="SV40ER reg"
Promoter 4204..4472
/gene="SV40 prom"
Rep_Origin 4371..4448
/gene="SV40 origin"
misc_binding 4451..4463
/dbxref="REBASE:SfiI"
selectable_marker653..5488
/gene="Kan/Neo"
ORF 4654..5448
/sequence="ORF_1 rf(1)"
Marker 4657..5445
/gene="NTP_II marker"
misc_binding 5466..5471
/dbxref="REBASE:EcoRV"
Other Gene 5542..5594
/gene="delta_U3 other"
Other Gene 5595..5775
/gene="HIV-1_5_LTR other"
Rep_Origin 6481..7100
/gene="pBR322 origin"
Promoter 7753..7802
/gene="NEOKAN prom"
ORF 7891..8685
/sequence="ORF_2 rf(1)"
Marker 7894..8682
/gene="NTP_II marker"
Rep_Origin 8790..9095
/gene="f1 origin"
BASE COUNT 2305 a 2221 c 2585 g 2058 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct 
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag 
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt 
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag 
3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct 
3061 tggtaccgga tccactagtg aattctgccc tgcgcccgca acccgagccg cacccgccgc 
3121 ggacggagcc catgcgcggg gcgaaccgcg cgcccccgcc cccgccccgc cccggcctcg 
3181 gccccggccc tggccccggg ggcagtcgcg cctgtgaacg gtggggcagg agaccctgta 
3241 ggaggacccc gggccgcagg cccctgagga gcgatgacgg aatataagct ggtggtggtg 
3301 ggcgccggcg gtgtgggcaa gagtgcgctg accatccagc tgatccagaa ccattttgtg 
3361 gacgaatacg accccactat agaggattcc taccggaagc aggtggtcat tgatggggag 
3421 acgtgcctgt tggacatcct ggataccgcc ggccaggagg agtacagcgc catgcgggac 
3481 cagtacatgc gcaccgggga gggcttcctg tgtgtgtttg ccatcaacaa caccaagtct 
3541 tttgaggaca tccaccagta cagggagcag atcaaacggg tgaaggactc ggatgacgtg 
3601 cccatggtgc tggtggggaa caagtgtgac ctggctgcac gcactgtgga atctcggcag 
3661 gctcaggacc tcgcccgaag ctacggcatc ccctacatcg agacctcggc caagacccgg 
3721 cagggagtgg aggatgcctt ctacacgttg gtgcgtgaga tccggcagca caagctgcgg 
3781 aagctgaacc ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc 
3841 tgacgcagca caagctcagg acatggaggt gccggatgca ggaaggaggt gcagacggaa 
3901 ggaggaggaa ggaaggacgg aagcaaggaa ggaaggaagg gctgctggag cccagtcacc 
3961 ccgggaccgt gggccgaggt gactgcagac cctcccaggg aggctgtgca cagactgtct 
4021 tgaacatccc aaatgccacc ggaaccccag cccttagctc ccctcccagg cctctgtggg 
4081 cccttgtcgg gcacagatgg gatcacagta aattattgga tggtcttgaa aaaaaaaaaa 
4141 aaaaaactcg agcatcatca ccatcaccat tgatgatccg gctgtggaat gtgtgtcagt 
4201 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
4261 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
4321 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
4381 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
4441 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
4501 gaggcctagt gaccagatct aatgcgttct aatgcgtacc agatctaatg cgtgctagcg 
4561 accagatcta atgcgtgcta ggcccttttg caaaaagctt gggctgcagg tcgaggcgga 
4621 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 
4681 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 
4741 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 
4801 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 
4861 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 
4921 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 
4981 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 
5041 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 
5101 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 
5161 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc 
5221 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 
5281 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 
5341 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 
5401 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 
5461 ggttcgatat ctagcgacca gatctaattg cgtgcaaggc agctgtagat cttagccact 
5521 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc 
5581 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 
5641 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 
5701 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 
5761 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 
5821 agaaatgaat atcagagagt gagaggaact tgggcctgac cagatctaat gcgtaggccg 
5881 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg 
5941 ggcgcgatcg ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 
6001 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 
6061 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 
6121 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca 
6181 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 
6241 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 
6301 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
6901 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
7561 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 
7621 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 
7681 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 
7741 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 
7801 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 
7861 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 
7921 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 
7981 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 
8041 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 
8101 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 
8161 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 
8221 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 
8281 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 
8341 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 
8401 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 
8461 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 
8521 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 
8581 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 
8641 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 
8701 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 
8761 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 
8821 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 
8881 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 
8941 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 
9001 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 
9061 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 
9121 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt 
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

Encap other(162,310)>>> 
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

amp prom(365,393)<<< 
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

HpaI 
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG

541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT

601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtct 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAATTACATCAGA

661 tatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa 720
ATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTT

721 ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgcctta 780
CCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGCACGGAAT

781 ttaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgc 840
AATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGGCGTAACG

HIV-1_5_LTR other(880,1060)>>> 
|
841 agagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccag 900
TCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACCAATCTGGTC

901 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 960
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG

961 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 1020
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT

1021 tccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggact 1080
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGA

1081 tgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgc 1140
ACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCG

HIV-1_psi_pack other(1171,1215)>>> 
|
1141 gcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag 1200
CGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTC

1201 gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgc 1260
CGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCG

NruI 
|
1261 gatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatag 1320
CTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATATC

1321 tatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcag 1380
ATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTC

1381 aaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac 1440
TTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTG

1441 ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataa 1500
AATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCTCTATT

1501 aagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccg 1560
TTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGC

1561 cacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaag 1620
GTGTCGTTCGCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACTCTTC

1621 tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc 1680
ACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGTGGTTCCG

RRE reg(1723,1956)>>> 
|
1681 aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgg 1740
TTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGAACC

ORF_3 rf(2)(1766,2449)>>> 
|
1741 gttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggc 1800
CAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCG

1801 cagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc 1860
GTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGATAACTCCG

1861 gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct 1920
CGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTTCTTAGGA

1921 ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaa 1980
CCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTT

1981 actcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaaca 2040
TGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAGACCTTGT

2041 gatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt 2100
CTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTGTTCGAA

2101 aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt 2160
TTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAA

2161 ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggta 2220
CCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGACACCAT

2221 tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgt 2280
ATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAAACGACA

2281 actttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct 2340
TGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGA

2341 cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga 2400
GGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCTCTCTCT

2401 cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagtt 2460
GTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTATTCGAACCCTCAA

2461 ccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgccc 2520
GGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGG

2521 attgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacg 2580
TAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGC

NdeI 
|
2581 tcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat 2640
AGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATA

2641 gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgccca 2700
CGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGT

2701 gtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctat 2760
CATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATA

2761 taccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacg 2820
ATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGC

2821 gggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca 2880
CCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGT

CMV prom(2911,2992)>>> 
|
2881 acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcg 2940
TGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGC

2941 tgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggag 3000
ACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTC

3001 acgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagct 3060
TGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCGATCGCAAATTTGAATTCGA

BamHI RasV12(3085,4146)<<< 
| |
KpnI EcoRI 
| | | |
3061 tggtaccggatccactagtgaattctgccctgcgcccgcaacccgagccgcacccgccgc 3120
ACCATGGCCTAGGTGATCACTTAAGACGGGACGCGGGCGTTGGGCTCGGCGTGGGCGGCG

3121 ggacggagcccatgcgcggggcgaaccgcgcgcccccgcccccgccccgccccggcctcg 3180
CCTGCCTCGGGTACGCGCCCCGCTTGGCGCGCGGGGGCGGGGGCGGGGCGGGGCCGGAGC

3181 gccccggccctggccccgggggcagtcgcgcctgtgaacggtggggcaggagaccctgta 3240
CGGGGCCGGGACCGGGGCCCCCGTCAGCGCGGACACTTGCCACCCCGTCCTCTGGGACAT

Ras reg(3274,3843)>>> 
|
3241 ggaggaccccgggccgcaggcccctgaggagcgatgacggaatataagctggtggtggtg 3300
CCTCCTGGGGCCCGGCGTCCGGGGACTCCTCGCTACTGCCTTATATTCGACCACCACCAC

3301 ggcgccgtcggtgtgggcaagagtgcgctgaccatccagctgatccagaaccattttgtg 3360
CCGCGGCAGCCACACCCGTTCTCACGCGACTGGTAGGTCGACTAGGTCTTGGTAAAACAC

3361 gacgaatacgaccccactatagaggattcctaccggaagcaggtggtcattgatggggag 3420
CTGCTTATGCTGGGGTGATATCTCCTAAGGATGGCCTTCGTCCACCAGTAACTACCCCTC

3421 acgtgcctgttggacatcctggataccgccggccaggaggagtacagcgccatgcgggac 3480
TGCACGGACAACCTGTAGGACCTATGGCGGCCGGTCCTCCTCATGTCGCGGTACGCCCTG

3481 cagtacatgcgcaccggggagggcttcctgtgtgtgtttgccatcaacaacaccaagtct 3540
GTCATGTACGCGTGGCCCCTCCCGAAGGACACACACAAACGGTAGTTGTTGTGGTTCAGA

3541 tttgaggacatccaccagtacagggagcagatcaaacgggtgaaggactcggatgacgtg 3600
AAACTCCTGTAGGTGGTCATGTCCCTCGTCTAGTTTGCCCACTTCCTGAGCCTACTGCAC

3601 cccatggtgctggtggggaacaagtgtgacctggctgcacgcactgtggaatctcggcag 3660
GGGTACCACGACCACCCCTTGTTCACACTGGACCGACGTGCGTGACACCTTAGAGCCGTC

3661 gctcaggacctcgcccgaagctacggcatcccctacatcgagacctcggccaagacccgg 3720
CGAGTCCTGGAGCGGGCTTCGATGCCGTAGGGGATGTAGCTCTGGAGCCGGTTCTGGGCC

3721 cagggagtggaggatgccttctacacgttggtgcgtgagatccggcagcacaagctgcgg 3780
GTCCCTCACCTCCTACGGAAGATGTGCAACCACGCACTCTAGGCCGTCGTGTTCGACGCC

3781 aagctgaaccctcctgatgagagtggccccggctgcatgagctgcaagtgtgtgctctcc 3840
TTCGACTTGGGAGGACTACTCTCACCGGGGCCGACGTACTCGACGTTCACACACGAGAGG

3841 tgacgcagcacaagctcaggacatggaggtgccggatgcaggaaggaggtgcagacggaa 3900
ACTGCGTCGTGTTCGAGTCCTGTACCTCCACGGCCTACGTCCTTCCTCCACGTCTGCCTT

3901 ggaggaggaaggaaggacggaagcaaggaaggaaggaagggctgctggagcccagtcacc 3960
CCTCCTCCTTCCTTCCTGCCTTCGTTCCTTCCTTCCTTCCCGACGACCTCGGGTCAGTGG

3961 ccgggaccgtgggccgaggtgactgcagaccctcccagggaggctgtgcacagactgtct 4020
GGCCCTGGCACCCGGCTCCACTGACGTCTGGGAGGGTCCCTCCGACACGTGTCTGACAGA

4021 tgaacatcccaaatgccaccggaaccccagcccttagctcccctcccaggcctctgtggg 4080
ACTTGTAGGGTTTACGGTGGCCTTGGGGTCGGGAATCGAGGGGAGGGTCCGGAGACACCC

ApaI 
|
4081 cccttgtcgggcacagatgggatcacagtaaattattggatggtcttgaaaaaaaaaaaa 4140
GGGAACAGCCCGTGTCTACCCTAGTGTCATTTAATAACCTACCAGAACTTTTTTTTTTTT

XhoI 6xHis tag(4153,4170)>>> SV40ER reg(4181,4417)<<< 
| | |
4141 aaaaaactcgagcatcatcaccatcaccattgatgatccggctgtggaatgtgtgtcagt 4200
TTTTTTGAGCTCGTAGTAGTGGTAGTGGTAACTACTAGGCCGACACCTTACACACAGTCA

SV40 prom(4204,4472)>>> 
|
4201 tagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca 4260
ATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGT

4261 attagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaa 4320
TAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTT

SV40 origin(4371,4448)>>> 
|
4321 gcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccc 4380
CGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGG

4381 taactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatg 4440
ATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATAC

SfiI 
|
4441 cagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttg 4500
GTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAAC

4501 gaggcctagtgaccagatctaatgcgttctaatgcgtaccagatctaatgcgtgctagcg 4560
CTCCGGATCACTGGTCTAGATTACGCAAGATTACGCATGGTCTAGATTACGCACGATCGC

4561 accagatctaatgcgtgctaggcccttttgcaaaaagcttgggctgcaggtcgaggcgga 4620
TGGTCTAGATTACGCACGATCCGGGAAAACGTTTTTCGAACCCGACGTCCAGCTCCGCCT

NTP_II marker(4657,5445)>>> 
|
ORF_1 rf(1)(4654,5448)>>> 
| |
Kan/Neo(4653,5488)>>> 
|| |
4621 tctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgca 4680
AGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGT

4681 ggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatc 4740
CCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAG

4741 ggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtc 4800
CCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAG

4801 aagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtgg 4860
TTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACC

4861 ctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagg 4920
GACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCC

4921 gactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcct 4980
CTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGA

4981 gccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggct 5040
CGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGA

5041 acctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaa 5100
TGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTT

5101 gccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaa 5160
CGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTT

5161 ctgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggc 5220
GACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCG

5221 gatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgt 5280
CTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACA

5281 ggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgct 5340
CCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGA

5341 gaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctccc 5400
CTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGG

5401 gattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctgg 5460
CTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCGCCCTGAGACC

EcoRV 
|
5461 ggttcgatatctagcgaccagatctaattgcgtgcaaggcagctgtagatcttagccact 5520
CCAAGCTATAGATCGCTGGTCTAGATTAACGCACGTTCCGTCGACATCTAGAATCGGTGA

delta_U3 other(5542,5594)>>> 
|
5521 ttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgc 5580
AAAATTTTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTTCTGTTCTAGACG

HIV-1_5_LTR other(5595,5775)>>> 
|
5581 tttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggct 5640
AAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGA

5641 aactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgt 5700
TTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACA

5701 gtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgt 5760
CACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACA

5761 ggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaa 5820
CCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTT

5821 agaaatgaatatcagagagtgagaggaacttgggcctgaccagatctaatgcgtaggccg 5880
TCTTTACTTATAGTCTCTCACTCTCCTTGAACCCGGACTGGTCTAGATTACGCATCCGGC

5881 tttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccg 5940
AAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAACGGGC

5941 ggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 6000
CCGCGCTAGCGACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC

6001 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 6060
AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG

6061 tggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatg 6120
ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTAC

6121 ctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgca 6180
GACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGT

6181 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 6240
AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT

6241 ttaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaata 6300
AATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTAT

6301 ccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct 6360
GGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA

6361 gcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggga 6420
CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT

6421 taacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc 6480
ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG

pBR322 origin(6481,7100)<<< 
|
6481 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg 6540
GCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGC

6541 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg 6600
GAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACC

6601 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt 6660
TTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAA

6661 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt 6720
AGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCA

6721 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg 6780
CATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGAC

6781 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact 6840
GCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGA

6841 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt 6900
CCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAA

6901 cttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctct 6960
GAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGA

6961 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac 7020
CGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTG

7021 cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc 7080
GCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAG

7081 tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacg 7140
AGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGC

7141 ttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta 7200
AATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAT

7201 aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacca 7260
TTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGT

7261 atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgc 7320
TACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACG

7321 ctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc 7380
GACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACG

7381 tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc 7440
ACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGG

7441 agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctat 7500
TCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATA

7501 taattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgt 7560
ATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACA

7561 tgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgc 7620
ACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACG

7621 tgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaag 7680
ACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTC

7681 agaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatgg 7740
TCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACC

NEOKAN prom(7753,7802)>>> 
|
7741 acagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgc 7800
TGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACG

7801 aaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctct 7860
TTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGA

NTP_II marker(7894,8682)>>> 
|
ORF_2 rf(1)(7891,8685)>>> 
| |
7861 gatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggt 7920
CTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCA

7921 tctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggc 7980
AGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCG

7981 tgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag 8040
ACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTC

8041 accgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctg 8100
TGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGAC

8101 gccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggac 8160
CGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTG

8161 tggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgcc 8220
ACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGG

8221 gagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacc 8280
CTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGG

8281 tgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagcc 8340
ACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGG

8341 ggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactg 8400
CCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGAC

8401 ttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgat 8460
AAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTA

8461 gcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggc 8520
CGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCG

8521 cggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaa 8580
GCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTT

8581 gagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgat 8640
CTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTA

8641 tcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaattt 8700
AGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAA

8701 ttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatc 8760
AACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAG

f1 origin(8790,9095)<<< 
|
8761 aaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactatt 8820
TTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAA

8821 aaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccact 8880
TTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGA

8881 acgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcg 8940
TGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGC

8941 gaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgag 9000
CTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTC

9001 aaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcac 9060
TTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTG

9061 gctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgc 9120
CGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCG

9121 cattcaggatcgaattaattcttaattaacatcatcaataatatacctt 9169
GTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA

                            LTR (1 total)
5'-LTR
Start: 670 End: 1151
Original Location Description:
670..1151
Misc. Feature (4 total)
CMV Promoter
Start: 2451 End: 3020
Original Location Description:
2451..3020
H-RasV12
Start: 3086 End: 4146
Neo/Kan
Start: 4654 End: 5448
Original Location Description:
3632..4426
3'-LTR
Start: 5570 End: 5859
Original Location Description:
4548..4837