pLenti-Myc T58A
| Vector Name | pLenti-Myc T58A |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV Sequencing Primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct 661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag 1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt 2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag 3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct 3061 tggtaccgga tccactagtg aattcggcac gaggcggcta gggtggaaga gccgggcgag 3121 cagagctgcg ctgcgggcgt cctgggaagg gagatccgga gcgaataggg ggcttcgcct 3181 ctggcccagc cctcccgctg atcccccagc cagcggtccg caacccttgc cgcatccacg 3241 aaactttgcc catagcagcg ggcgggcact ttgcactgga acttacaaca cccgagcaag 3301 gacgcgactc tcccgacgcg gggaggctat tctgcccatt tggggacact tccccgccgc 3361 tgccaggacc cgcttctctg aaaggctctc cttgcagctg cttagacgct ggattttttt 3421 cgggtagtgg aaaaccagcc tcccgcgacg atgcccctca acgttagctt caccaacagg 3481 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac 3541 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg 3601 aagaaattcg agctgctgcc cgctccgccc ctgtccccta gccgccgctc cgggctctgc 3661 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc 3721 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg 3781 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc 3841 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc 3901 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc 3961 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac 4021 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg 4081 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc 4141 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc 4201 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg 4261 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct 4321 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca 4381 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc 4441 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc 4501 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta 4561 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc 4621 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag 4681 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa 4741 cttgaacagc tacggaactc ttgtgcgtaa ggaaaagtaa ggaaaacgat tccttctaac 4801 agaaatgtcc tgagcaatca cctatgaact tgtttcaaat gcatgatcaa atgcaacctc 4861 acaaccttgg ctgagtcttg agactgaaag atttagccat aatgtaaact gcctcaaatt 4921 ggactttggg cataaaagaa cttttttatg cttaccatct tttttttttc tttaacagat 4981 ttgtatttaa gaattgtttt taaaaaattt taagatttac acaatgtttc tctgtaaata 5041 ttgccattaa atgtaaataa ctttaataaa acgtttatag cagttacaca gaatttcaat 5101 cctagtatat agtacctagt attataggta ctataaaccc taattttttt tatttaagta 5161 cattttgctt tttaaagttg attttttttt attgttttta gaaaaaataa aataactggc 5221 aaatataaaa aaaaaaaaaa aaaactcgac tctagagcgg ccgcaccgcg gagtactggg 5281 ccccctaggc tcgagcatca tcaccatcac cattgatgat ccggctgtgg aatgtgtgtc 5341 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 5401 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 5461 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 5521 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 5581 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 5641 ttggaggcct agtgaccaga tctaatgcgt aggccctttt gcaaaaagct tgggctgcag 5701 gtcgaggcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 5761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 5821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 5881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 5941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 6001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 6061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 6121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 6181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 6241 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 6301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 6361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 6421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 6481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 6541 cgggactctg gggttcgata tctagcgacc agatctaatt gcgtgcaagg cagctgtaga 6601 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 6661 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6841 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6901 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgggcctga ccagatctaa 6961 tgcgtaggcc gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 7021 gttgttgccc gggcgcgatc gctgcccctc ccccgtgcct tccttgaccc tggaaggtgc 7081 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 7141 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 7201 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcag 7261 atcgatctgc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt 7321 atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta 7381 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 7441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 8041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 8101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 8161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 8221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 8281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 8341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 8401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 8461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8641 cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc 8701 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg 8761 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg 8821 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg 8881 ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg 8941 gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 9001 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 9061 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 9121 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 9181 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 9241 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 9301 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 9361 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 9421 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 9481 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 9541 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 9601 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 9661 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 9721 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt 9781 tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc 9841 ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag 9901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 9961 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa 10021 gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 10081 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 10141 gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg 10201 cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
LOCUS dna 10260 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 880..1060 /gene="HIV-1_5_LTR other" Other Gene 1171..1215 /gene="HIV-1_psi_pack other" misc_binding 1257..1262 /dbxref="REBASE:NruI" Regulatory_Seq 1723..1956 /gene="RRE reg" ORF 1766..2449 /sequence="ORF_2 rf(2)" misc_binding 2636..2641 /dbxref="REBASE:NdeI" Promoter 2911..2992 /gene="CMV prom" misc_binding 3062..3067 /dbxref="REBASE:KpnI" misc_binding 3068..3073 /dbxref="REBASE:BamHI" misc_binding 3080..3085 /dbxref="REBASE:EcoRI" ORF 3451..4770 /sequence="ORF_1 rf(1)" misc_binding 5251..5256 /dbxref="REBASE:XbaI" misc_binding 5257..5264 /dbxref="REBASE:NotI" misc_binding 5278..5283 /dbxref="REBASE:ApaI" misc_binding 5290..5295 /dbxref="REBASE:XhoI" Tag 5296..5313 /gene="6xHis tag" Regulatory_Seq 5324..5560 /gene="SV40ER reg" Promoter 5347..5615 /gene="SV40 prom" Rep_Origin 5514..5591 /gene="SV40 origin" misc_binding 5594..5606 /dbxref="REBASE:SfiI" misc_binding 5645..5650 /dbxref="REBASE:StuI" ORF 5745..6539 /sequence="ORF_3 rf(3)" Marker 5748..6536 /gene="NTP_II marker" Other Gene 6633..6685 /gene="delta_U3 other" Other Gene 6686..6866 /gene="HIV-1_5_LTR other" misc_binding 7028..7033 /dbxref="REBASE:XmaI" misc_binding 7028..7033 /dbxref="REBASE:SmaI" Rep_Origin 7572..8191 /gene="pBR322 origin" Promoter 8844..8893 /gene="NEOKAN prom" ORF 8982..9776 /sequence="ORF_4 rf(3)" Marker 8985..9773 /gene="NTP_II marker" Rep_Origin 9881..10186 /gene="f1 origin" BASE COUNT 2625 a 2529 c 2753 g 2353 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct 661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag 1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt 2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag 3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct 3061 tggtaccgga tccactagtg aattcggcac gaggcggcta gggtggaaga gccgggcgag 3121 cagagctgcg ctgcgggcgt cctgggaagg gagatccgga gcgaataggg ggcttcgcct 3181 ctggcccagc cctcccgctg atcccccagc cagcggtccg caacccttgc cgcatccacg 3241 aaactttgcc catagcagcg ggcgggcact ttgcactgga acttacaaca cccgagcaag 3301 gacgcgactc tcccgacgcg gggaggctat tctgcccatt tggggacact tccccgccgc 3361 tgccaggacc cgcttctctg aaaggctctc cttgcagctg cttagacgct ggattttttt 3421 cgggtagtgg aaaaccagcc tcccgcgacg atgcccctca acgttagctt caccaacagg 3481 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac 3541 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg 3601 aagaaattcg agctgctgcc cgctccgccc ctgtccccta gccgccgctc cgggctctgc 3661 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc 3721 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg 3781 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc 3841 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc 3901 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc 3961 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac 4021 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg 4081 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc 4141 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc 4201 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg 4261 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct 4321 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca 4381 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc 4441 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc 4501 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta 4561 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc 4621 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag 4681 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa 4741 cttgaacagc tacggaactc ttgtgcgtaa ggaaaagtaa ggaaaacgat tccttctaac 4801 agaaatgtcc tgagcaatca cctatgaact tgtttcaaat gcatgatcaa atgcaacctc 4861 acaaccttgg ctgagtcttg agactgaaag atttagccat aatgtaaact gcctcaaatt 4921 ggactttggg cataaaagaa cttttttatg cttaccatct tttttttttc tttaacagat 4981 ttgtatttaa gaattgtttt taaaaaattt taagatttac acaatgtttc tctgtaaata 5041 ttgccattaa atgtaaataa ctttaataaa acgtttatag cagttacaca gaatttcaat 5101 cctagtatat agtacctagt attataggta ctataaaccc taattttttt tatttaagta 5161 cattttgctt tttaaagttg attttttttt attgttttta gaaaaaataa aataactggc 5221 aaatataaaa aaaaaaaaaa aaaactcgac tctagagcgg ccgcaccgcg gagtactggg 5281 ccccctaggc tcgagcatca tcaccatcac cattgatgat ccggctgtgg aatgtgtgtc 5341 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 5401 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 5461 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 5521 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 5581 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 5641 ttggaggcct agtgaccaga tctaatgcgt aggccctttt gcaaaaagct tgggctgcag 5701 gtcgaggcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 5761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 5821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 5881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 5941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 6001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 6061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 6121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 6181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 6241 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 6301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 6361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 6421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 6481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 6541 cgggactctg gggttcgata tctagcgacc agatctaatt gcgtgcaagg cagctgtaga 6601 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 6661 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6841 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6901 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgggcctga ccagatctaa 6961 tgcgtaggcc gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 7021 gttgttgccc gggcgcgatc gctgcccctc ccccgtgcct tccttgaccc tggaaggtgc 7081 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 7141 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 7201 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcag 7261 atcgatctgc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt 7321 atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta 7381 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 7441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 8041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 8101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 8161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 8221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 8281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 8341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 8401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 8461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8641 cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc 8701 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg 8761 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg 8821 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg 8881 ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg 8941 gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 9001 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 9061 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 9121 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 9181 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 9241 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 9301 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 9361 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 9421 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 9481 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 9541 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 9601 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 9661 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 9721 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt 9781 tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc 9841 ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag 9901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 9961 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa 10021 gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 10081 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 10141 gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg 10201 cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt //
S dna 10260 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 880..1060 /gene="HIV-1_5_LTR other" Other Gene 1171..1215 /gene="HIV-1_psi_pack other" misc_binding 1257..1262 /dbxref="REBASE:NruI" Regulatory_Seq 1723..1956 /gene="RRE reg" ORF 1766..2449 /sequence="ORF_2 rf(2)" misc_binding 2636..2641 /dbxref="REBASE:NdeI" Promoter 2911..2992 /gene="CMV prom" misc_binding 3062..3067 /dbxref="REBASE:KpnI" misc_binding 3068..3073 /dbxref="REBASE:BamHI" misc_binding 3080..3085 /dbxref="REBASE:EcoRI" ORF 3451..4770 /sequence="ORF_1 rf(1)" misc_binding 5251..5256 /dbxref="REBASE:XbaI" misc_binding 5257..5264 /dbxref="REBASE:NotI" misc_binding 5278..5283 /dbxref="REBASE:ApaI" misc_binding 5290..5295 /dbxref="REBASE:XhoI" Tag 5296..5313 /gene="6xHis tag" Regulatory_Seq 5324..5560 /gene="SV40ER reg" Promoter 5347..5615 /gene="SV40 prom" Rep_Origin 5514..5591 /gene="SV40 origin" misc_binding 5594..5606 /dbxref="REBASE:SfiI" misc_binding 5645..5650 /dbxref="REBASE:StuI" ORF 5745..6539 /sequence="ORF_3 rf(3)" Marker 5748..6536 /gene="NTP_II marker" Other Gene 6633..6685 /gene="delta_U3 other" Other Gene 6686..6866 /gene="HIV-1_5_LTR other" misc_binding 7028..7033 /dbxref="REBASE:XmaI" misc_binding 7028..7033 /dbxref="REBASE:SmaI" Rep_Origin 7572..8191 /gene="pBR322 origin" Promoter 8844..8893 /gene="NEOKAN prom" ORF 8982..9776 /sequence="ORF_4 rf(3)" Marker 8985..9773 /gene="NTP_II marker" Rep_Origin 9881..10186 /gene="f1 origin" BASE COUNT 2625 a 2529 c 2753 g 2353 t 0 others ORIGIN