pLenti-Multi-gRNA-PGK-Neo
| Vector Name | pLenti-Multi-gRNA-PGK-Neo |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Neomycin/G418 |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg 4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat 4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt 4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg 4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg 4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggttttag 4501 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 4561 agtcggtgct ttttttctcg agagctagcg aattcccacg gggttggggt tgcgcctttt 4621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg 4681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat 4741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga 4801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag 4861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt 4921 ggccaatagc ggctgctcag cggggcgcgc cgagagcagc ggccgggaag gggcggtgcg 4981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt 5041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct 5101 ctctccccag gggaggaccg gtcgcgatcg ccatggctga acaagatgga ttgcacgcag 5161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 5221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 5281 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 5341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 5401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 5461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 5521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 5581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 5641 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg 5701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 5761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 5821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 5881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtt taaacgtcga 5941 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6301 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga 6541 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6601 aagaaaaggg gggactggaa gggctaattc actcccaacg agacaagatc tgctttttgc 6661 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6841 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6901 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7021 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 7201 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 7261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 7321 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 7381 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa 7441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 7501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 7561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 7621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 7681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 7741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 7801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 7861 cgtttacaat ttcc
LOCUS dna 7874 bp
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_1 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 5133..5927
/sequence="ORF_2 rf(3)"
Marker 5138..5924
/gene="NTP_II marker"
misc_binding 5310..5315
/dbxref="REBASE:PstI"
misc_binding 6545..6550
/dbxref="REBASE:KpnI"
Other Gene 6616..6667
/gene="delta_U3 other"
Other Gene 6668..6848
/gene="HIV-1_5_LTR other"
Rep_Origin 7060..7137
/gene="SV40 origin"
misc_binding 7140..7152
/dbxref="REBASE:SfiI"
misc_binding 7191..7196
/dbxref="REBASE:StuI"
Promoter 7228..7246
/gene="T7 prom"
Reporter 7254..7412
/gene="lacZ_a reporter"
Rep_Origin 7434..7740
/gene="f1 origin"
BASE COUNT 1955 a 1912 c 2074 g 1933 t 0 others
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg
4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat
4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggttttag
4501 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
4561 agtcggtgct ttttttctcg agagctagcg aattcccacg gggttggggt tgcgcctttt
4621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
4681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
4741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
4801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
4861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
4921 ggccaatagc ggctgctcag cggggcgcgc cgagagcagc ggccgggaag gggcggtgcg
4981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
5041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
5101 ctctccccag gggaggaccg gtcgcgatcg ccatggctga acaagatgga ttgcacgcag
5161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
5221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
5281 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
5341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
5401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
5461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
5521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
5581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
5641 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
5701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
5761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
5821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
5881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtt taaacgtcga
5941 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
6001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
6061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
6121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
6181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
6241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
6301 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
6361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
6421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
6481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
6541 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
6601 aagaaaaggg gggactggaa gggctaattc actcccaacg agacaagatc tgctttttgc
6661 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
6721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
6781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
6841 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
6901 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
6961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
7021 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
7141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
7201 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
7261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
7321 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
7381 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa
7441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
7501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
7561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
7621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
7681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
7741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
7801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
7861 cgtttacaat ttcc
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 caggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac 60
GTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATG
amp prom(62,90)>>>
|
61 attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa 120
TAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTT
ORF_1 rf(3)(132,992)>>>
|
amp marker(132,992)>>>
|
121 aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat 180
TTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTA
181 tttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatc 240
AAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAG
241 agttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgaga 300
TCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCT
301 gttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcg 360
CAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGC
361 cggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctc 420
GCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAG
421 agaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacag 480
TCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTC
481 taagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttc 540
ATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAG
541 tgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatg 600
ACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTAC
601 taactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtg 660
ATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCAC
661 acaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac 720
TGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATG
721 ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggac 780
AATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTG
781 cacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg 840
GTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCAC
841 agcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcg 900
TCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGC
901 tagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg 960
ATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGAC
961 agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatac 1020
TCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATG
1021 tttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttg 1080
AAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAAC
1081 ataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccg 1140
TATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGC
pBR322 origin(1147,1766)>>>
|
1141 tagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgc 1200
ATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACG
1201 aaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactc 1260
TTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAG
1261 tttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgt 1320
AAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACA
1321 agccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgc 1380
TCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACG
1381 taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggact 1440
ATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGA
1441 caagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacac 1500
GTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTG
1501 agcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgag 1560
TCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTC
1561 aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcg 1620
TTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGC
1621 gaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctg 1680
CTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGAC
1681 tcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgga 1740
AGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCT
1741 gcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcctt 1800
CGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAA
1801 ttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcct 1860
AACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGA
1861 ttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcg 1920
AACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGC
1921 aggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt 1980
TCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAA
1981 aatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatta 2040
TTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAAT
lac prom(2075,2104)>>>
|
2041 atgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgta 2100
TACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCAT
2101 tgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatt 2160
ACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAA
T3 prom(2174,2193)>>>
|
2161 acgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagcttaat 2220
TGCGGTTCGCGCGTTAATTGGGAGTGATTTCCCTTGTTTTCGACCTCGACGTTCGAATTA
2221 gtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgc 2280
CATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACG
2281 cttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcg 2340
GAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGC
2341 tgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgcc 2400
ACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGG
HIV-1_5_LTR other(2448,2628)>>>
|
2401 gcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggt 2460
CGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTTATTTGCCCAGAGAGACCA
2461 tagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctc 2520
ATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAG
2521 aataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggta 2580
TTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCAT
2581 actagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaa 2640
TGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTT
2641 cagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctga 2700
GTCCCTGGACTTTCGCTTTCCCTTTGGTCTCGAGAGAGCTGCGTCCTGAGCCGAACGACT
HIV-1_psi_pack other(2736,2780)>>>
|
2701 agcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactag 2760
TCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATC
2761 cggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattag 2820
GCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATC
2821 atcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaaca 2880
TAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGT
2881 tatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac 2940
ATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTG
2941 atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcaga 3000
TAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCT
3001 agaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggataga 3060
TCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCT
3061 gataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagac 3120
CTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTG
NotI
|
3121 caccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaatt 3180
GTGGCGTGTCGTTCGCCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAA
3181 ggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccca 3240
CCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGT
RRE reg(3290,3523)>>>
|
3241 ccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgt 3300
GGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACA
3301 tccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacgg 3360
AGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGGAGTTACTGCGACTGCC
3361 tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggcta 3420
ATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGAT
3421 ttgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaa 3480
AACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTT
3481 gaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgct 3540
CTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGA
3541 ctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctc 3600
GACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAG
3601 tggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacac 3660
ACCTTGTCTAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTG
3661 aagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaaga 3720
TTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCT
3721 attattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggct 3780
TAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGA
3781 gtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttt 3840
CACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAA
3841 tgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagac 3900
ACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTG
3901 ccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggaga 3960
GGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCT
HpaI
|
3961 gagagacagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttt 4020
CTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCCAATTGAAA
4021 taaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagc 4080
ATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCATCTGTATTATCG
ClaI
|
4081 aacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttATcga 4140
TTGTCTGTATGTTTGATTTCTTAATGTTTTTGTTTAATGTTTTTAAGTTTTAAAATAGCT
4141 tcacgagactagcCTCGAGCCCCAGTGGAAAGACGCGCAGGCAAAACGCACCACGTGACG 4200
AGTGCTCTGATCGGAGCTCGGGGTCACCTTTCTGCGCGTCCGTTTTGCGTGGTGCACTGC
hU6 prom(4229,4485)>>>
|
4201 GAGCGTGACCGCGCGCCGAGCGCGCGCCAAGGTCGGGCAGGAAGAGGGCCTATTTCCCAT 4260
CTCGCACTGGCGCGCGGCTCGCGCGCGGTTCCAGCCCGTCCTTCTCCCGGATAAAGGGTA
4261 GATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTT 4320
CTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAA
4321 GACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGG 4380
CTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACC
NdeI
|
4381 GTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTG 4440
CATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAAC
4441 AAAGTATTTCGATTTCTTGGGTTTATATATCTTGTGGAAAGGACGAAACACCGGttttag 4500
TTTCATAAAGCTAAAGAACCCAAATATATAGAACACCTTTCCTGCTTTGTGGCCAAAATC
4501 agctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccg 4560
TCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGC
4561 agtcggtgcTTTTTTTctcgagAGCTAGCGAATTCCCACGGGGTTGGGGTTGCGCCTTTT 4620
TCAGCCACGAAAAAAAGAGCTCTCGATCGCTTAAGGGTGCCCCAACCCCAACGCGGAAAA
4621 CCAAGGCAGCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACG 4680
GGTTCCGTCGGGACCCAAACGCGTCCCTGCGCCGACGAGACCCGCACCAAGGCCCTTTGC
4681 CAGCGGCGCCGACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGAT 4740
GTCGCCGCGGCTGGGACCCAGAGCGTGTAAGAAGTGCAGGCAAGCGTCGCAGTGGGCCTA
4741 CTTCGCCGCTACCCTTGTGGGCCCCCCGGCGACGCTTCCTGCTCCGCCCCTAAGTCGGGA 4800
GAAGCGGCGATGGGAACACCCGGGGGGCCGCTGCGAAGGACGAGGCGGGGATTCAGCCCT
4801 AGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACGTCTCACTAG 4860
TCCAAGGAACGCCAAGCGCCGCACGGCCTGCACTGTTTGCCTTCGGCGTGCAGAGTGATC
4861 TACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGT 4920
ATGGGAGCGTCTGCCTGTCGCGGTCCCTCGTTACCGTCGCGCGGCTGGCGCTACCCGACA
4921 GGCCAATAGCGGCTGCTCAGCGGGGCGCGCCGAGAGCAGCGGCCGGGAAGGGGCGGTGCG 4980
CCGGTTATCGCCGACGAGTCGCCCCGCGCGGCTCTCGTCGCCGGCCCTTCCCCGCCACGC
4981 GGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGCCCGCGCGGTGTTCCGCATT 5040
CCTCCGCCCCACACCCCGCCATCACACCCGGGACAAGGACGGGCGCGCCACAAGGCGTAA
5041 CTGCAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCT 5100
GACGTTCGGAGGCCTCGCGTGCAGCCGTCAGCCGAGGGAGCAACTGGCTTAGTGGCTGGA
NTP_II marker(5138,5924)>>>
|
ORF_2 rf(3)(5133,5927)>>>
| |
5101 CTCTCCCCAGGGGAGGACCGGTCGCGATCGCCAtggctgaacaagatggattgcacgcag 5160
GAGAGGGGTCCCCTCCTGGCCAGCGCTAGCGGTACCGACTTGTTCTACCTAACGTGCGTC
5161 gttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcg 5220
CAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGC
5221 gctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtca 5280
CGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGT
PstI
|
5281 agaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggc 5340
TCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCG
5341 tggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggg 5400
ACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCC
5401 actggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctg 5460
TGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGAC
5461 ccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggcta 5520
GGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGAT
5521 cctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 5580
GGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTC
5581 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaac 5640
GGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTG
5641 tgttcgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcg 5700
ACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTACTAGAGCAGCACTGGGTACCGC
5701 atgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtg 5760
TACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACAC
5761 gccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctg 5820
CGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGAC
5821 aagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccg 5880
TTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGC
5881 attcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaGTTTAAACGtcga 5940
TAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCAAATTTGCAGCT
5941 caatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgc 6000
GTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACG
6001 tccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccg 6060
AGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGC
6061 tatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagtt 6120
ATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAA
6121 gtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccac 6180
CACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTG
6181 tggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccc 6240
ACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGG
6241 tattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggct 6300
ATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGA
6301 gttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgct 6360
CAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGA
6361 cgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccct 6420
GCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGA
6421 caatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtct 6480
GTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGA
6481 cgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattcga 6540
GCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACCTTAAGCT
KpnI
|
6541 gctcggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaa 6600
CGAGCCATGGAAATTCTGGTTACTGAATGTTCCGTCGACATCTAGAATCGGTGAAAAATT
delta_U3 other(6616,6667)>>>
|
6601 aagaaaaggggggactggaagggctaattcactcccaacgagacaagatctgctttttgc 6660
TTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTCTGTTCTAGACGAAAAACG
HIV-1_5_LTR other(6668,6848)>>>
|
6661 ttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagg 6720
AACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCC
6721 gaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccg 6780
CTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGC
6781 tctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaat 6840
AGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTA
6841 ctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatg 6900
GAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTAC
6901 aatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaa 6960
TTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTT
6961 tagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtc 7020
ATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAG
SV40 origin(7060,7137)>>>
|
7021 caaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgccc 7080
GTTTGAGTAGTTACATAGAATAGTACAGACCGAGATCGATAGGGCGGGGATTGAGGCGGG
7081 agttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgag 7140
TCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTC
SfiI StuI
| |
7141 gccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggc 7200
CGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCG
T7 prom(7228,7246)<<< lacZ_a reporter(7254,7412)>>>
| |
7201 ttttgcgtcgagacgtacccaattcgccctatagtgagtcgtattacgcgcgctcactgg 7260
AAAACGCAGCTCTGCATGGGTTAAGCGGGATATCACTCAGCATAATGCGCGCGAGTGACC
7261 ccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttg 7320
GGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAAC
7321 cagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccctt 7380
GTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAA
f1 origin(7434,7740)>>>
|
7381 cccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaa 7440
GGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATT
7441 gcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgc 7500
CGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCG
7501 ccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaag 7560
GGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTC
7561 ctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacccca 7620
GAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGT
7621 aaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttc 7680
TTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAG
7681 gccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaa 7740
CGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTT
7741 cactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcct 7800
GTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGA
7801 attggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaa 7860
TAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATT
7861 cgtttacaatttcc 7874
GCAAATGTTAAAGG
LOCUS dna 7874 bp
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_1 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 5133..5927
/sequence="ORF_2 rf(3)"
Marker 5138..5924
/gene="NTP_II marker"
misc_binding 5310..5315
/dbxref="REBASE:PstI"
misc_binding 6545..6550
/dbxref="REBASE:KpnI"
Other Gene 6616..6667
/gene="delta_U3 other"
Other Gene 6668..6848
/gene="HIV-1_5_LTR other"
Rep_Origin 7060..7137
/gene="SV40 origin"
misc_binding 7140..7152
/dbxref="REBASE:SfiI"
misc_binding 7191..7196
/dbxref="REBASE:StuI"
Promoter 7228..7246
/gene="T7 prom"
Reporter 7254..7412
/gene="lacZ_a reporter"
Rep_Origin 7434..7740
/gene="f1 origin"
BASE COUNT 1955 a 1912 c 2074 g 1933 t 0 others