pLenti-III-miR-Off
| Vector Name | pLenti-III-miR-Off |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | H1 Forward: 5'-CAACCCGCTCCAAGGAATCG-3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg 1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg 1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat 1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc 2041 tgtatgagac cacaaaaata gtcttctcta gaagctagca gatatcagtc gacactcgag 2101 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 2161 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 2221 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 2281 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 2341 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 2401 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 2461 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 2521 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 2581 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 2641 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag 2701 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2761 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2821 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2881 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 2941 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3001 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3061 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3121 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3181 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3241 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3301 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 3361 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3421 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg 3481 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg 3541 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc 3601 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc 3661 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 3721 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 3781 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 3841 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 3901 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 3961 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac 4021 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4081 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct 4141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 4321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 4381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 4441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 4501 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 4561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 4621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc 4681 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa 4741 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 4801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 4861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 4921 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 4981 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5161 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg 5221 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg 5281 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6121 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt 6181 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg 6241 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt 6301 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct 6361 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg 6421 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt 6481 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 6541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 6601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 6661 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 6721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 7021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 7081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 7141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 7201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 7261 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca 7321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 7381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 7441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 7501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 7561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 7621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 7681 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 7741 taacatcatc aataatatac cttttttgga ttgaagccaa tatgataatg agggggtgga 7801 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt 7861 gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt 7921 tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg cggttttagg cggatgttgt 7981 agtaaatttg ggcgtaaccg agtaagattt ggccattttc gcgggaaaac tgaataagag 8041 gaagtgaaat ctgaataatt ttgtgttact catagcgcgt aatactctca tgagcggata 8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8161 agtgccacct gacgtaacta taacggtcct aaggtagcga aaatgtagtc ttatgcaata 8221 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa 8281 aagcaccgtg catgccgatt ggtggaagta aggtggtacg at
FEATURES Location/Qualifiers source 1..8322 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2155..2460 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2461..2664 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" CDS 2719..3435 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2719 .. 2721 / #05fd14 2: 2722 .. 2724 / #05fd14 / 1a 3: 2725 .. 3435 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3517..4119 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4132..4719 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4603..4614) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4729..4962 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4782..4962 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5034..5155 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5484..6072) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6484..7278 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg 1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg 1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat 1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc 2041 tgtatgagac cacaaaaata gtcttctcta gaagctagca gatatcagtc gacactcgag 2101 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 2161 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 2221 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 2281 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 2341 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 2401 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 2461 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 2521 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 2581 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 2641 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag 2701 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2761 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2821 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2881 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 2941 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3001 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3061 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3121 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3181 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3241 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3301 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 3361 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3421 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg 3481 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg 3541 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc 3601 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc 3661 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 3721 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 3781 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 3841 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 3901 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 3961 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac 4021 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4081 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct 4141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 4321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 4381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 4441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 4501 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 4561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 4621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc 4681 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa 4741 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 4801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 4861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 4921 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 4981 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5161 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg 5221 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg 5281 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6121 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt 6181 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg 6241 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt 6301 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct 6361 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg 6421 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt 6481 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 6541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 6601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 6661 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 6721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 7021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 7081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 7141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 7201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 7261 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca 7321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 7381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 7441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 7501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 7561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 7621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 7681 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 7741 taacatcatc aataatatac cttttttgga ttgaagccaa tatgataatg agggggtgga 7801 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt 7861 gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt 7921 tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg cggttttagg cggatgttgt 7981 agtaaatttg ggcgtaaccg agtaagattt ggccattttc gcgggaaaac tgaataagag 8041 gaagtgaaat ctgaataatt ttgtgttact catagcgcgt aatactctca tgagcggata 8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8161 agtgccacct gacgtaacta taacggtcct aaggtagcga aaatgtagtc ttatgcaata 8221 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa 8281 aagcaccgtg catgccgatt ggtggaagta aggtggtacg at
FEATURES Location/Qualifiers source 1..8322 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2155..2460 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2461..2664 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" CDS 2719..3435 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2719 .. 2721 / #05fd14 2: 2722 .. 2724 / #05fd14 / 1a 3: 2725 .. 3435 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3517..4119 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4132..4719 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4603..4614) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4729..4962 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4782..4962 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5034..5155 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5484..6072) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6484..7278 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"