pLenti-III-mir-GFP-Cloning
| Vector Name | pLenti-III-mir-GFP-Cloning |
| VectorType | Lentiviral miRNA Vector |
| Sequencing Primers | EGFP-C Sequencing Primer: 5'--CAT GGT CCT GCT GGA GTT CGT G--3' Reverse Sequencing Primer: 5'--GAC TTT CCA CAC CTG GTT GCT GAC --3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagaatc tagaggtacc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
3121 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
3181 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
3241 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
3301 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
3361 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
3421 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
3481 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
3541 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
3601 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
3661 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
3721 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
3781 tcggcatgga cgagctgtac aagtgaggta ccgatatcga attcatagct agccctgcag
3841 gtctagactc gaggcggccg cagtcgagta cccatacgac gtcccagact acgcttgagt
3901 ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3961 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4021 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4141 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
4201 gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
4261 gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
4321 accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
4381 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
4441 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
4501 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct cctggcgccg
4561 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
4621 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
4681 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
4741 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
4801 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga
4861 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
4921 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
4981 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
5041 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
5101 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
5161 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
5221 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
5281 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
5341 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt
5401 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc
5461 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
5521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
5581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
5641 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
5701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga
5881 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac
5941 gatcccggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6001 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6061 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6601 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
6661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
6721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
6841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
6901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
6961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
7021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
7081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
7141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
7201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac
7261 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
7321 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
7381 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
7441 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
7501 tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg
7561 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
7621 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
7681 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
7741 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
7801 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
7861 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
7921 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
7981 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
8041 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
8101 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
8161 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
8221 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
8281 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
8341 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat
8401 tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa tagaccgcga
8461 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
8521 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca
8581 aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc
8641 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
8701 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
8761 cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gatcgaatta
8821 attcttaatt aacatcatca ataatatacc tt
LOCUS dna 8852 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3806
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
Reporter 3087..3803
/gene="EGFP reporter"
misc_binding 3813..3818
/dbxref="REBASE:EcoRV"
misc_binding 3819..3824
/dbxref="REBASE:EcoRI"
misc_binding 3835..3840
/dbxref="REBASE:PstI"
misc_binding 3848..3853
/dbxref="REBASE:XhoI"
misc_binding 3854..3861
/dbxref="REBASE:NotI"
Promoter 3913..4181
/gene="SV40 prom"
Rep_Origin 4080..4157
/gene="SV40 origin"
ORF 4147..4815
/sequence="ORF_2 rf(1)"
misc_binding 4160..4172
/dbxref="REBASE:SfiI"
Marker 4216..4815
/gene="puro marker"
Other Gene 5425..5477
/gene="delta_U3 other"
Other Gene 5478..5658
/gene="HIV-1_5_LTR other"
Rep_Origin 6164..6783
/gene="pBR322 origin"
Promoter 7436..7485
/gene="NEOKAN prom"
misc_binding 7543..7548
/dbxref="REBASE:BclI"
ORF 7574..8368
/sequence="ORF_3 rf(2)"
Marker 7577..8365
/gene="NTP_II marker"
Rep_Origin 8473..8778
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_4 rf(3)(2901,3806)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
EGFP reporter(3087,3803)>>>
|
3061 agctagaatctagaggtaccgccaccatggtgagcaagggcgaggagctgttcaccgggg 3120
TCGATCTTAGATCTCCATGGCGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCC
3121 tggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccg 3180
ACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGC
3181 gcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg 3240
CGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGC
3241 gcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgct 3300
CGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGA
3301 tcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaag 3360
AGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTC
3361 gctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccg 3420
CGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGC
3421 aggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca 3480
TCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGT
3481 aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtct 3540
TCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGA
3541 atatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca 3600
TATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGT
3601 tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacg 3660
AGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGC
3661 gccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacc 3720
CGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGG
3721 ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactc 3780
GGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAG
EcoRI
|
EcoRV
| |
3781 tcggcatggacgagctgtacaagtgaggtaccgatatcgaattcatagctagccctgcag 3840
AGCCGTACCTGCTCGACATGTTCACTCCATGGCTATAGCTTAAGTATCGATCGGGACGTC
PstI XhoI NotI
| | |
3841 gtctagactcgaggcggccgcagtcgagtacccatacgacgtcccagactacgcttgagt 3900
CAGATCTGAGCTCCGCCGGCGTCAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCA
SV40 prom(3913,4181)>>>
|
3901 ttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagca 3960
AATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGT
3961 tgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaa 4020
ACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTT
4021 gtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgccca 4080
CATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGT
SV40 origin(4080,4157)>>>
|
4081 tcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttt 4140
AGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAA
SfiI
|
ORF_2 rf(1)(4147,4815)>>>
| |
4141 ttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggag 4200
AATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTC
puro marker(4216,4815)>>>
|
4201 gcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgac 4260
CGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTG
4261 gtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccac 4320
CAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTG
4321 accgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacg 4380
TGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGC
4381 cgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtc 4440
GCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAG
4441 tggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatg 4500
ACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTAC
4501 gccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgccg 4560
CGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGC
4561 caccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccag 4620
GTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTC
4621 ggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggg 4680
CCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCC
4681 gtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggc 4740
CACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCG
4741 ttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgc 4800
AAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCG
4801 aagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaaga 4860
TTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCT
4861 ttgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatg 4920
AACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTAC
4921 cctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcc 4980
GGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGG
4981 tggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgc 5040
ACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACG
5041 actgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctt 5100
TGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAA
5101 tccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcctt 5160
AGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAA
5161 gcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgggg 5220
CGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCC
5221 aagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacg 5280
TTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGC
5281 tccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctg 5340
AGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGAC
5341 ccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcccttt 5400
GGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAA
delta_U3 other(5425,5477)>>>
|
5401 gggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatc 5460
CCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAG
HIV-1_5_LTR other(5478,5658)>>>
|
5461 tgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctg 5520
ACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGAC
5521 gctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtag 5580
CGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATC
5581 tgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcag 5640
ACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTC
5641 tgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttg 5700
ACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAAC
5701 caaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttaca 5760
GTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGT
5761 aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagtt 5820
TTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAA
5821 gtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcgga 5880
CACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCT
5881 gaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaac 5940
CTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTG
5941 gatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctctt 6000
CTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAA
6001 ccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag 6060
GGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTC
6061 ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaaca 6120
GAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGT
pBR322 origin(6164,6783)<<<
|
6121 tgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttt 6180
ACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAA
6181 tccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggc 6240
AGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCG
6241 gaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct 6300
CTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGA
6301 ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcg 6360
GAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGC
6361 tggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcca 6420
ACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGT
6421 agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaact 6480
TCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGA
6481 atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggta 6540
TAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCAT
6541 acaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggccta 6600
TGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGAT
6601 actacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacct 6660
TGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGA
6661 tcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt 6720
AGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAA
6721 tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttga 6780
AAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACT
6781 tcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtca 6840
AGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGT
6841 tgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaat 6900
ACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTA
6901 caatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgagg 6960
GTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCC
6961 cacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgt 7020
GTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACA
7021 agataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgag 7080
TCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTC
7081 acccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagc 7140
TGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCG
7141 gcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaag 7200
CGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTC
7201 ctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcac 7260
GATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTG
7261 ctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgt 7320
GATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACA
7321 cagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttg 7380
GTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAAC
NEOKAN prom(7436,7485)>>>
|
7381 cagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccgga 7440
GTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCT
7441 attgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggc 7500
TAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCG
BclI
|
7501 tttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatg 7560
AAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTAC
NTP_II marker(7577,8365)>>>
|
ORF_3 rf(2)(7574,8368)>>>
| |
7561 aggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggt 7620
TCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCA
7621 ggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgt 7680
CCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCA
7681 gttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgc 7740
CAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACG
7741 cctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttcc 7800
GGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGG
7801 ttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcga 7860
AACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCT
7861 agtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcat 7920
TCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTA
7921 ggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccacca 7980
CCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGT
7981 agcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagga 8040
TCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCT
8041 tgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggc 8100
ACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCG
8101 gagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatat 8160
CTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATA
8161 catggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcgga 8220
GTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCT
8221 ccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatg 8280
GGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTAC
8281 ggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcctt 8340
CCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAA
8341 ctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcat 8400
GATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTA
8401 tttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcga 8460
AAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCT
f1 origin(8473,8778)<<<
|
8461 tagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactcca 8520
ATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGT
8521 acgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccca 8580
TGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGT
8581 aatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcc 8640
TTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGG
8641 cccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaag 8700
GGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTC
8701 cgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccacca 8760
GCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGT
8761 cacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaatta 8820
GTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAAT
8821 attcttaattaacatcatcaataatatacctt 8852
TAAGAATTAATTGTAGTAGTTATTATATGGAA