pLenti-III-UBC
| Vector Name | pLenti-III-UBC |
| VectorType | Lentiviral vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | UBC Sequencing Primer 5'---TGAAGCTCCGGTTTTGAACT ---3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagaggcc tccgcgccgg gttttggcgc ctcccgcggg
1861 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
1921 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
1981 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
2041 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
2101 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
2161 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
2221 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
2281 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
2341 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
2401 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
2461 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
2521 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
2581 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
2641 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
2701 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
2761 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
2821 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
2881 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
2941 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
3001 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagcggatca attgagtact
3061 tacgtaggta ccccagtgtg gtggcctgca ggtgaattca ctagtaccgg taggcctgtc
3121 gacgatatcg ggcccgcggc cgctggatcc tctagactgc agctcgagta cccatacgac
3181 gtcccagact acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc
3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
3301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
3361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
3421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
3481 attccagaag tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc
3541 gcctcgccac ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg
3601 actaccccgc cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc
3661 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
3721 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
3781 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
3841 tggaagggct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
3901 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
3961 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
4021 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
4081 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg
4141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
4201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
4261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
4321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
4381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
4441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
4501 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
4561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
4621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca
4681 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca
4741 ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
4801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
4861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
4921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
4981 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
5041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
5101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
5161 gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct
5221 gcattaatga atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa
5281 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
5881 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
6061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
6121 cacgttaagg gattttggcc atgagattat caaaaaggat cttcacctag atccttttca
6181 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
6241 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
6301 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
6361 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
6421 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
6481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
6541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
6601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
6661 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
6721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
6781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
6841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
6901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
6961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
7021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
7081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
7141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
7201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
7261 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
7321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
7381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
7441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
7501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
7561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
7621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
7681 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac
7741 atcatcaata atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt
7801 gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga
7861 tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt
7921 gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta
7981 aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag
8041 tgaaatctga ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata
8101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
8161 ccacctgacg taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct
8221 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
8281 accgtgcatg ccgattggtg gaagtaaggt ggtacgat
//
LOCUS dna 8318 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
Promoter 1827..3038
/gene="hUbC prom"
misc_binding 3067..3072
/dbxref="REBASE:KpnI"
misc_binding 3094..3099
/dbxref="REBASE:EcoRI"
misc_binding 3118..3123
/dbxref="REBASE:HincII"
misc_binding 3124..3129
/dbxref="REBASE:EcoRV"
misc_binding 3130..3135
/dbxref="REBASE:ApaI"
misc_binding 3136..3143
/dbxref="REBASE:NotI"
misc_binding 3145..3150
/dbxref="REBASE:BamHI"
misc_binding 3151..3156
/dbxref="REBASE:XbaI"
misc_binding 3163..3168
/dbxref="REBASE:XhoI"
Promoter 3213..3481
/gene="SV40 prom"
Rep_Origin 3380..3457
/gene="SV40 origin"
ORF 3447..4115
/sequence="ORF_2 rf(3)"
misc_binding 3460..3472
/dbxref="REBASE:SfiI"
Marker 3516..4115
/gene="puro marker"
Other Gene 4725..4777
/gene="delta_U3 other"
Other Gene 4778..4958
/gene="HIV-1_5_LTR other"
Rep_Origin 5465..6084
/gene="pBR322 origin"
Promoter 6342..6391
/gene="NEOKAN prom"
misc_binding 6449..6454
/dbxref="REBASE:BclI"
ORF 6480..7274
/sequence="ORF_3 rf(3)"
Marker 6483..7271
/gene="NTP_II marker"
Rep_Origin 7379..7685
/gene="f1 origin"
Other Gene 7740..7841
/gene="RITR other"
Other Gene 7740..7841
/gene="LITR other"
Other Gene 7921..8069
/gene="Encap other"
BASE COUNT 1980 a 2027 c 2394 g 1917 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagaggcc tccgcgccgg gttttggcgc ctcccgcggg
1861 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
1921 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
1981 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
2041 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
2101 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
2161 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
2221 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
2281 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
2341 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
2401 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
2461 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
2521 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
2581 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
2641 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
2701 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
2761 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
2821 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
2881 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
2941 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
3001 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagcggatca attgagtact
3061 tacgtaggta ccccagtgtg gtggcctgca ggtgaattca ctagtaccgg taggcctgtc
3121 gacgatatcg ggcccgcggc cgctggatcc tctagactgc agctcgagta cccatacgac
3181 gtcccagact acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc
3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
3301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
3361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
3421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
3481 attccagaag tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc
3541 gcctcgccac ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg
3601 actaccccgc cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc
3661 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
3721 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
3781 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
3841 tggaagggct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
3901 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
3961 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
4021 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
4081 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg
4141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
4201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
4261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
4321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
4381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
4441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
4501 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
4561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
4621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca
4681 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca
4741 ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
4801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
4861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
4921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
4981 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
5041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
5101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
5161 gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct
5221 gcattaatga atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa
5281 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
5881 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
6061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
6121 cacgttaagg gattttggcc atgagattat caaaaaggat cttcacctag atccttttca
6181 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
6241 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
6301 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
6361 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
6421 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
6481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
6541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
6601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
6661 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
6721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
6781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
6841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
6901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
6961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
7021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
7081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
7141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
7201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
7261 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
7321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
7381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
7441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
7501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
7561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
7621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
7681 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac
7741 atcatcaata atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt
7801 gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga
7861 tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt
7921 gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta
7981 aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag
8041 tgaaatctga ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata
8101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
8161 ccacctgacg taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct
8221 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
8281 accgtgcatg ccgattggtg gaagtaaggt ggtacgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
hUbC prom(1827,3038)>>>
|
1801 aaattttcgggtttttcgaacctagaggcctccgcgccgggttttggcgcctcccgcggg 1860
TTTAAAAGCCCAAAAAGCTTGGATCTCCGGAGGCGCGGCCCAAAACCGCGGAGGGCGCCC
1861 cgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctga 1920
GCGGGGGGAGGAGTGCCGCTCGCGACGGTGCAGTCTGCTTCCCGCGTCGCTCGCAGGACT
1921 tccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaacc 1980
AGGAAGGCGGGCCTGCGAGTCCTGTCGCCGGGCGACGAGTATTCTGAGCCGGAATCTTGG
1981 ccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggtttt 2040
GGTCATAGTCGTCTTCCTGTAAAATCCTGCCCTGAACCCACTGAGATCCCGTGACCAAAA
2041 ctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggaggg 2100
GAAAGGTCTCTCGCCTTGTCCGCTCCTTTTCATCAGGGAAGAGCCGCTAAGACGCCTCCC
2101 atctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagct 2160
TAGAGGCACCCCGCCACTTGCGGCTACTAATATATTCCTGCGCGGCCCACACCGTGTCGA
2161 agttccgtcgcagccgggatttgggtcgcggttcttgtttgtggatcgctgtgatcgtca 2220
TCAAGGCAGCGTCGGCCCTAAACCCAGCGCCAAGAACAAACACCTAGCGACACTAGCAGT
2221 cttggtgagtagcgggctgctgggctggccggggctttcgtggccgccgggccgctcggt 2280
GAACCACTCATCGCCCGACGACCCGACCGGCCCCGAAAGCACCGGCGGCCCGGCGAGCCA
2281 gggacggaagcgtgtggagagaccgccaagggctgtagtctgggtccgcgagcaaggttg 2340
CCCTGCCTTCGCACACCTCTCTGGCGGTTCCCGACATCAGACCCAGGCGCTCGTTCCAAC
2341 ccctgaactgggggttggggggagcgcagcaaaatggcggctgttcccgagtcttgaatg 2400
GGGACTTGACCCCCAACCCCCCTCGCGTCGTTTTACCGCCGACAAGGGCTCAGAACTTAC
2401 gaagacgcttgtgaggcgggctgtgaggtcgttgaaacaaggtggggggcatggtgggcg 2460
CTTCTGCGAACACTCCGCCCGACACTCCAGCAACTTTGTTCCACCCCCCGTACCACCCGC
2461 gcaagaacccaaggtcttgaggccttcgctaatgcgggaaagctcttattcgggtgagat 2520
CGTTCTTGGGTTCCAGAACTCCGGAAGCGATTACGCCCTTTCGAGAATAAGCCCACTCTA
2521 gggctggggcaccatctggggaccctgacgtgaagtttgtcactgactggagaactcggt 2580
CCCGACCCCGTGGTAGACCCCTGGGACTGCACTTCAAACAGTGACTGACCTCTTGAGCCA
2581 ttgtcgtctgttgcgggggcggcagttatggcggtgccgttgggcagtgcacccgtacct 2640
AACAGCAGACAACGCCCCCGCCGTCAATACCGCCACGGCAACCCGTCACGTGGGCATGGA
2641 ttgggagcgcgcgccctcgtcgtgtcgtgacgtcacccgttctgttggcttataatgcag 2700
AACCCTCGCGCGCGGGAGCAGCACAGCACTGCAGTGGGCAAGACAACCGAATATTACGTC
2701 ggtggggccacctgccggtaggtgtgcggtaggcttttctccgtcgcaggacgcagggtt 2760
CCACCCCGGTGGACGGCCATCCACACGCCATCCGAAAAGAGGCAGCGTCCTGCGTCCCAA
2761 cgggcctagggtaggctctcctgaatcgacaggcgccggacctctggtgaggggagggat 2820
GCCCGGATCCCATCCGAGAGGACTTAGCTGTCCGCGGCCTGGAGACCACTCCCCTCCCTA
2821 aagtgaggcgtcagtttctttggtcggttttatgtacctatcttcttaagtagctgaagc 2880
TTCACTCCGCAGTCAAAGAAACCAGCCAAAATACATGGATAGAAGAATTCATCGACTTCG
2881 tccggttttgaactatgcgctcggggttggcgagtgtgttttgtgaagttttttaggcac 2940
AGGCCAAAACTTGATACGCGAGCCCCAACCGCTCACACAAAACACTTCAAAAAATCCGTG
2941 cttttgaaatgtaatcatttgggtcaatatgtaattttcagtgttagactagtaaattgt 3000
GAAAACTTTACATTAGTAAACCCAGTTATACATTAAAAGTCACAATCTGATCATTTAACA
3001 ccgctaaattctggccgtttttggcttttttgttagacgctagcggatcaattgagtact 3060
GGCGATTTAAGACCGGCAAAAACCGAAAAAACAATCTGCGATCGCCTAGTTAACTCATGA
KpnI EcoRI
| |
3061 tacgtaggtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctgtc 3120
ATGCATCCATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAG
EcoRV NotI
| |
HincII ApaI BamHI XbaI XhoI
| | | | | | |
3121 gacgatatcgggcccgcggccgctggatcctctagactgcagctcgagtacccatacgac 3180
CTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTG
SV40 prom(3213,3481)>>>
|
3181 gtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagc 3240
CAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCG
3241 aggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtcccc 3300
TCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGG
3301 aggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagt 3360
TCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCA
SV40 origin(3380,3457)>>>
|
3361 cccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcc 3420
GGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGG
SfiI
|
ORF_2 rf(3)(3447,4115)>>>
| |
3421 ccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagct 3480
GGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGA
puro marker(3516,4115)>>>
|
3481 attccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgc 3540
TAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACG
3541 gcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccg 3600
CGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGC
3601 actaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagc 3660
TGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCG
3661 tgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacg 3720
ACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGC
3721 acggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcg 3780
TGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGC
3781 ccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacaga 3840
GGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCT
3841 tggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcg 3900
ACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGC
3901 gcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtgg 3960
CGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACC
3961 aggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcc 4020
TCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGG
4021 ccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgc 4080
GGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCG
4081 gcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctgg 4140
CGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACC
4141 attacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctat 4200
TAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATA
4201 gtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattt 4260
CACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAA
4261 tctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtca 4320
AGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGT
4321 ggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattg 4380
CCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAAC
4381 ccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcgg 4440
GGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCC
4441 aactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgaca 4500
TTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGT
4501 attccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgcca 4560
TAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGT
4561 cctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggacc 4620
GGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGG
4621 ttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctca 4680
AAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGT
delta_U3 other(4725,4777)>>>
|
4681 gacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattca 4740
CTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGT
HIV-1_5_LTR other(4778,4958)>>>
|
4741 ctcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagat 4800
GAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTA
4801 ctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagctt 4860
GACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAA
4861 gccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatc 4920
CGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAG
4921 cctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttat 4980
GGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATA
4981 tattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttat 5040
ATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATA
5041 tgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatt 5100
ACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAA
5101 tttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctg 5160
AAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGAC
5161 gcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtct 5220
CGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGA
5221 gcattaatgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaa 5280
CGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTT
5281 aataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttc 5340
TTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAG
5341 ggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcag 5400
CCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTC
5401 gggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaa 5460
CCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTT
pBR322 origin(5465,6084)<<<
|
5461 aggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc 5520
TCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAG
5521 gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccc 5580
CTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGG
5581 ctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccg 5640
GACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGC
5641 cctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtt 5700
GGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAA
5701 cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgacc 5760
GCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGG
5761 gctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgc 5820
CGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCG
5821 cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacag 5880
GTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTC
5881 agttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcg 5940
TCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGC
5941 ctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaa 6000
GAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTT
6001 ccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaag 6060
GGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTC
6061 gatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact 6120
CTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGA
6121 cacgttaagggattttggccatgagattatcaaaaaggatcttcacctagatccttttca 6180
GTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGT
6181 cgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggcta 6240
GCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGAT
6241 tctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacat 6300
AGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTA
NEOKAN prom(6342,6391)>>>
|
6301 ggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctgggg 6360
CCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCC
6361 cgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaa 6420
GCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTT
BclI
|
6421 ggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgca 6480
CCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGT
NTP_II marker(6483,7271)>>>
|
ORF_3 rf(3)(6480,7274)>>>
| |
6481 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcg 6540
ACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGC
6541 gctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcag 6600
CGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTC
6601 cgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgc 6660
GCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACG
6661 aagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgc 6720
TTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACG
6721 tcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcagg 6780
AGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCC
6781 atctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgc 6840
TAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACG
6841 ggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgca 6900
CCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGT
6901 tcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaag 6960
AGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTC
6961 agcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacg 7020
TCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGC
7021 gcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatg 7080
CGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTAC
7081 gccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggaca 7140
CGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGT
7141 tagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcc 7200
ATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGG
7201 tcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttg 7260
AGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAAC
7261 acgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaatag 7320
TGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATC
f1 origin(7379,7685)<<<
|
7321 gccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgtt 7380
CGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAA
7381 gttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcga 7440
CAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCT
7441 aaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttg 7500
TTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAAC
7501 gggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagct 7560
CCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGA
7561 tgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggc 7620
ACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCG
7621 gctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgctt 7680
CGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAA
7681 aatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaac 7740
TTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTG
RITR other(7740,7841)<<<
|
LITR other(7740,7841)>>>
|
7741 atcatcaataatataccttttttggattgaagccaatatgataatgagggggtggagttt 7800
TAGTAGTTATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAA
7801 gtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtga 7860
CACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACT
7861 tgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggt 7920
ACAACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCA
Encap other(7921,8069)>>>
|
7921 gtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagta 7980
CACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCAT
7981 aatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaag 8040
TTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTC
8041 tgaaatctgaataattttgtgttactcatagcgcgtaatactctcatgagcggatacata 8100
ACTTTAGACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTAT
8101 tttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtg 8160
AAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCAC
8161 ccacctgacgtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactct 8220
GGTGGACTGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGA
8221 tgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagc 8280
ACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCG
8281 accgtgcatgccgattggtggaagtaaggtggtacgat 8318
TGGCACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 8318 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
Promoter 1827..3038
/gene="hUbC prom"
misc_binding 3067..3072
/dbxref="REBASE:KpnI"
misc_binding 3094..3099
/dbxref="REBASE:EcoRI"
misc_binding 3118..3123
/dbxref="REBASE:HincII"
misc_binding 3124..3129
/dbxref="REBASE:EcoRV"
misc_binding 3130..3135
/dbxref="REBASE:ApaI"
misc_binding 3136..3143
/dbxref="REBASE:NotI"
misc_binding 3145..3150
/dbxref="REBASE:BamHI"
misc_binding 3151..3156
/dbxref="REBASE:XbaI"
misc_binding 3163..3168
/dbxref="REBASE:XhoI"
Promoter 3213..3481
/gene="SV40 prom"
Rep_Origin 3380..3457
/gene="SV40 origin"
ORF 3447..4115
/sequence="ORF_2 rf(3)"
misc_binding 3460..3472
/dbxref="REBASE:SfiI"
Marker 3516..4115
/gene="puro marker"
Other Gene 4725..4777
/gene="delta_U3 other"
Other Gene 4778..4958
/gene="HIV-1_5_LTR other"
Rep_Origin 5465..6084
/gene="pBR322 origin"
Promoter 6342..6391
/gene="NEOKAN prom"
misc_binding 6449..6454
/dbxref="REBASE:BclI"
ORF 6480..7274
/sequence="ORF_3 rf(3)"
Marker 6483..7271
/gene="NTP_II marker"
Rep_Origin 7379..7685
/gene="f1 origin"
Other Gene 7740..7841
/gene="RITR other"
Other Gene 7740..7841
/gene="LITR other"
Other Gene 7921..8069
/gene="Encap other"
BASE COUNT 1980 a 2027 c 2394 g 1917 t 0 others