pLenti-III-RFP-N
| Vector Name | pLenti-III-RFP-N |
| VectorType | Plasmid |
| Antibiotic Information | Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 gtatataacg ccaccatggt gagcgagctg attaaggaga acatgcacat gaagctgtac 2521 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc 2581 tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc 2641 ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag 2701 ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc 2761 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc 2821 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag 2881 aagaaaacac tcggctggga ggcctccacc gagaccctgt accccgctga cggcggcctg 2941 gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg 3001 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat 3061 gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac 3121 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacag acccggggct 3181 agccaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga attcactagt 3241 accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag actgcagctc 3301 gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt gtggaaagtc 3361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3481 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3541 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3601 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt 3661 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg 3721 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg 3781 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 3841 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 3901 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 3961 ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4021 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 4081 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4141 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4201 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg 4261 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4321 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4381 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4441 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4501 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4561 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4621 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 4681 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4741 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4801 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat 4861 ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct 4921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 4981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 5101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 5161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5281 atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat 5341 gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa ataccgcaca 5401 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 5461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6241 agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa aggatcttca 6301 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 6361 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 6421 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 6481 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 6541 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 6601 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 6661 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 6721 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 6781 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 6841 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 6901 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 6961 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 7021 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 7081 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 7141 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 7201 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 7261 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 7321 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 7381 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca 7441 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 7501 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 7561 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 7621 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 7681 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 7741 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 7801 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat 7861 taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca atatgataat 7921 gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt 7981 gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa 8041 agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag 8101 gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa 8161 ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatactctc 8221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 8281 tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg aaaatgtagt 8341 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac 8401 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gat //
FEATURES Location/Qualifiers source 1..8453 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 2476..3171 /codon_start=1 /product="monomeric far-red fluorescent protein" /note="mKate2" /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY CDLPSKLGHR" CDS 3304..3330 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3515..3650 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3651..4250 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4263..4850 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4734..4745) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4860..5093 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4913..5093 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5165..5286 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5615..6203) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6615..7409 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 gtatataacg ccaccatggt gagcgagctg attaaggaga acatgcacat gaagctgtac 2521 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc 2581 tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc 2641 ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag 2701 ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc 2761 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc 2821 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag 2881 aagaaaacac tcggctggga ggcctccacc gagaccctgt accccgctga cggcggcctg 2941 gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg 3001 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat 3061 gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac 3121 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacag acccggggct 3181 agccaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga attcactagt 3241 accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag actgcagctc 3301 gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt gtggaaagtc 3361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3481 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3541 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3601 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt 3661 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg 3721 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg 3781 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 3841 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 3901 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 3961 ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4021 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 4081 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4141 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4201 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg 4261 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4321 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4381 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4441 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4501 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4561 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4621 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 4681 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4741 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4801 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat 4861 ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct 4921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 4981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 5101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 5161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5281 atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat 5341 gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa ataccgcaca 5401 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 5461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6241 agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa aggatcttca 6301 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 6361 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 6421 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 6481 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 6541 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 6601 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 6661 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 6721 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 6781 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 6841 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 6901 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 6961 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 7021 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 7081 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 7141 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 7201 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 7261 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 7321 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 7381 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca 7441 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 7501 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 7561 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 7621 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 7681 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 7741 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 7801 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat 7861 taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca atatgataat 7921 gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt 7981 gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa 8041 agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag 8101 gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa 8161 ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatactctc 8221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 8281 tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg aaaatgtagt 8341 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac 8401 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gat //
FEATURES Location/Qualifiers source 1..8453 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 2476..3171 /codon_start=1 /product="monomeric far-red fluorescent protein" /note="mKate2" /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY CDLPSKLGHR" CDS 3304..3330 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3515..3650 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3651..4250 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4263..4850 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4734..4745) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4860..5093 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4913..5093 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5165..5286 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5615..6203) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6615..7409 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"