pLenti-III-RFP-C

1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actcgaggtg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac 2641 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac 2701 ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct tcgacatcct 2761 ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga 2821 cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca catacgaaga 2881 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa 2941 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact 3001 cggctgggag gcctccaccg agaccctgta ccccgctgac ggcggcctgg aaggcagagc 3061 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata 3121 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag 3181 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt 3241 ggccagatac tgcgacctcc ctagcaaact ggggcacaga taagtcgagt acccatacga 3301 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag 3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3601 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg 3661 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc 3721 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag 3781 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 3841 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 3901 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 3961 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4021 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 4081 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4141 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4201 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4261 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4321 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4381 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4441 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4501 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4561 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4621 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 4681 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 4741 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 4801 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 4861 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 4921 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 4981 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 5041 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 5101 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5161 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5281 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5341 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5401 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 5821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 5881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 5941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6001 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6241 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6301 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6361 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6421 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6481 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6541 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6601 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 6661 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 6721 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 6781 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6841 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6901 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6961 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 7021 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 7081 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7141 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7201 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7261 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7321 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7381 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7441 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7501 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7561 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7621 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 7681 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 7741 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 7801 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 7861 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 7921 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 7981 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 8041 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 8101 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8161 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8221 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8281 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8341 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8401 caccgtgcat gccgattggt ggaagtaagg tggtacgat

FEATURES Location/Qualifiers source 1..8439 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 2588..3283 /codon_start=1 /product="monomeric far-red fluorescent protein" /note="mKate2" /note="mammalian codon-optimized" /note="color: #ff0000" /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC DLPSKLGHR" CDS 3290..3316 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3501..3636 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3637..4236 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4249..4836 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4720..4731) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4846..5079 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4899..5079 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5151..5272 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5601..6189) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6601..7395 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actcgaggtg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac 2641 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac 2701 ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct tcgacatcct 2761 ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga 2821 cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca catacgaaga 2881 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa 2941 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact 3001 cggctgggag gcctccaccg agaccctgta ccccgctgac ggcggcctgg aaggcagagc 3061 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata 3121 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag 3181 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt 3241 ggccagatac tgcgacctcc ctagcaaact ggggcacaga taagtcgagt acccatacga 3301 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag 3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3601 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg 3661 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc 3721 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag 3781 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 3841 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 3901 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 3961 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4021 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 4081 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4141 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4201 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4261 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4321 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4381 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4441 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4501 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4561 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4621 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 4681 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 4741 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 4801 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 4861 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 4921 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 4981 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 5041 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 5101 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5161 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5281 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5341 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5401 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 5821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 5881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 5941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6001 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6241 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6301 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6361 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6421 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6481 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6541 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6601 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 6661 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 6721 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 6781 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6841 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6901 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6961 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 7021 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 7081 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7141 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7201 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7261 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7321 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7381 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7441 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7501 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7561 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7621 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 7681 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 7741 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 7801 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 7861 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 7921 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 7981 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 8041 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 8101 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8161 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8221 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8281 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8341 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8401 caccgtgcat gccgattggt ggaagtaagg tggtacgat