pLenti-III-Promoterless
| Vector Name | pLenti-III-Promoterless |
| VectorType | Lentiviral vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | Insulator sequence located upstream of MCS to suppress any 5'LTR promoter activity |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga 1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca 1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt 1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc 2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac 2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg 2161 cagcagcgac cgcccggggc tccgctccgg tccggcgctc cccccgcatc ccgagccggc 2221 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc 2281 ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaga attgggggag 2341 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta 2401 gggggcagca gcgaccgccc ggggctccgc tccggtccgg cgctcccccc catcccgagc 2461 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc ttcctctgaa 2521 cgcttctcgc tgctctttga gcctgcagac acctggggga tacggggaaa aacactgggg 2581 tacctacgta agggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta 2641 cgtccctccc cgctaggggg cagcagcgac cgcccggggc tccgctccgg tccggcgctc 2701 cccccgcatc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg 2761 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tgggggatac 2821 ggggaaaaga attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg 2881 gatcctctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa 2941 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3241 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 3301 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 3361 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 3421 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 3481 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 3541 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg 3601 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 3661 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 3721 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 3781 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 3841 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac 3901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 3961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 4201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 4261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 4321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 4381 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc 4441 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt 4501 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 4561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 4621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 4681 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 4741 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 4861 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag 4921 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg 4981 tcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag 5881 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg 5941 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc 6001 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt 6061 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc 6121 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag 6181 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 6241 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 6301 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 6361 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 6421 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 6481 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 6541 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 6601 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 6661 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 6721 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 6781 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 6841 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6901 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6961 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa 7021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 7081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 7141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 7201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 7261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 7321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 7381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca 7441 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg 7501 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac 7561 ggggcgggtg acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg 7621 taagcgacgg atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga 7681 caattttcgc gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt 7741 tggccatttt cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac 7801 tcatagcgcg taatactctc atgagcggat acatatttga atgtatttag aaaaataaac 7861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc 7921 taaggtagcg aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat 7981 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt 8041 aaggtggtac gat
FEATURES Location/Qualifiers source 1..8053 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" CDS 2904..2930 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3115..3250 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3251..3850 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 3863..4450 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4334..4345) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4460..4693 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4513..4693 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 4765..4886 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5215..5803) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6215..7009 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga 1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca 1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt 1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc 2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac 2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg 2161 cagcagcgac cgcccggggc tccgctccgg tccggcgctc cccccgcatc ccgagccggc 2221 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc 2281 ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaga attgggggag 2341 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta 2401 gggggcagca gcgaccgccc ggggctccgc tccggtccgg cgctcccccc catcccgagc 2461 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc ttcctctgaa 2521 cgcttctcgc tgctctttga gcctgcagac acctggggga tacggggaaa aacactgggg 2581 tacctacgta agggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta 2641 cgtccctccc cgctaggggg cagcagcgac cgcccggggc tccgctccgg tccggcgctc 2701 cccccgcatc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg 2761 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tgggggatac 2821 ggggaaaaga attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg 2881 gatcctctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa 2941 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3241 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 3301 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 3361 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 3421 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 3481 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 3541 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg 3601 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 3661 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 3721 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 3781 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 3841 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac 3901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 3961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 4201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 4261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 4321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 4381 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc 4441 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt 4501 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 4561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 4621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 4681 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 4741 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 4861 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag 4921 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg 4981 tcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag 5881 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg 5941 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc 6001 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt 6061 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc 6121 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag 6181 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 6241 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 6301 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 6361 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 6421 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 6481 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 6541 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 6601 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 6661 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 6721 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 6781 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 6841 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6901 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6961 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa 7021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 7081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 7141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 7201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 7261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 7321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 7381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca 7441 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg 7501 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac 7561 ggggcgggtg acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg 7621 taagcgacgg atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga 7681 caattttcgc gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt 7741 tggccatttt cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac 7801 tcatagcgcg taatactctc atgagcggat acatatttga atgtatttag aaaaataaac 7861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc 7921 taaggtagcg aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat 7981 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt 8041 aaggtggtac gat
FEATURES Location/Qualifiers source 1..8053 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" CDS 2904..2930 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3115..3250 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3251..3850 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 3863..4450 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4334..4345) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4460..4693 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4513..4693 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 4765..4886 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5215..5803) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6215..7009 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN