pLenti-III-HSVtk

Vector Name pLenti-III-HSVtk
VectorType Lentivirus
Antibiotic Information Puromycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 aatggcttcg taccccggcc atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg 2521 cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt 2581 ccgcccggag cagaaaatgc ccacgctact gcgggtttat atagacggtc cccacgggat 2641 ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta 2701 cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat 2761 ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg cggcggtggt 2821 aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg ccgttctggc 2881 tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc tcaccctcat 2941 cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcggt accttatggg 3001 cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga ccttgcccgg 3061 caccaacatc gtgcttgggg cccttccgga ggacagacac atcgaccgcc tggccaaacg 3121 ccagcgcccc ggcgagcggc tggacctggc tatgctggct gcgattcgcc gcgtttacgg 3181 gctacttgcc aatacggtgc ggtatctgca gtgcggcggg tcgtggcggg aggactgggg 3241 acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca acgcgggccc 3301 acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt tgctggcccc 3361 caacggcgac ctgtataacg tgtttgcctg ggccttggac gtcttggcca aacgcctccg 3421 ttccatgcac gtctttatcc tggattacga ccaatcgccc gccggctgcc gggacgccct 3481 gctgcaactt acctccggga tggtccagac ccacgtcacc acccccggct ccataccgac 3541 gatatgcgac ctggcgcgca cgtttgcccg ggagatgggg gaggctaact gactcgagca 3601 attcgttaac ctcgagcggg atcaattccg ccccccccct aacgttactg gccgaagccg 3661 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 3721 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 3781 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 3841 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 3901 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 3961 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 4021 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 4081 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 4141 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggtga 4201 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 4261 taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc 4321 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 4381 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 4441 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 4501 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 4561 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 4621 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 4681 aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 4741 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 4801 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 4861 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taatgaatta 4921 attaagaatt atcaccgctt ctattcagcc agtaaggcct gtctttaatg gcctccggca 4981 tgagacactt tctagagtcg acgatatcgg gcccgcggcc gctggatcct ctagactgca 5041 gctcgagtac ccatacgacg tcccagacta cgcttgagtt taaacacgcg tggtgtggaa 5101 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 5161 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 5221 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 5281 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 5341 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc 5401 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc 5461 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac 5521 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5581 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5641 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5701 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg 5761 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 5821 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5881 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5941 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt 6001 tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 6421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6541 cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc 6601 ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct tgtactgggt 6661 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6721 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6781 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6841 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6901 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7021 atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa tgaaatggca 7081 ttatgggtat tatgggtctg cattaatgaa tcggccaacg gatgtcggtg tgaaataccg 7141 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7981 gctcagtgga acgaaaactc acgttaaggg attttggcca tgagattatc aaaaaggatc 8041 ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg 8101 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta 8161 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa 8221 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg 8281 atggctttct tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca 8341 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 8401 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 8461 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 8521 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 8581 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 8641 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 8701 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 8761 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 8821 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 8881 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 8941 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 9001 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 9061 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 9121 gccttctatc gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag 9181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 9241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 9301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 9361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 9421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 9481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 9541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 9601 gaattaattc ttaattaaca tcatcaataa tatacctttt ttggattgaa gccaatatga 9661 taatgagggg gtggagtttg tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag 9721 tagtgtggcg gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg 9781 caaaagtgac gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt 9841 ttaggcggat gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg 9901 aaaactgaat aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatac 9961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10021 cacatttccc cgaaaagtgc cacctgacgt aactataacg gtcctaaggt agcgaaaatg 10081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 10141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgat
                            LOCUS dna 10197 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" Regulatory_Seq 2416..2455 /gene="tetO reg" ORF 2462..3592 /sequence="ORF_4 rf(2)" misc_binding 3567..3572 /dbxref="REBASE:XmaI" misc_binding 3567..3572 /dbxref="REBASE:SmaI" misc_binding 3605..3610 /dbxref="REBASE:HpaI" Regulatory_Seq 3631..4187 /gene="IRES reg" Regulatory_Seq 3694..4187 /gene="ires_emcv reg" Reporter 4194..4910 /gene="EGFP reporter" ORF 4194..4913 /sequence="ORF_5 rf(3)" misc_binding 4955..4960 /dbxref="REBASE:StuI" misc_binding 5015..5022 /dbxref="REBASE:NotI" misc_binding 5024..5029 /dbxref="REBASE:BamHI" Promoter 5092..5360 /gene="SV40 prom" Rep_Origin 5259..5336 /gene="SV40 origin" ORF 5326..5994 /sequence="ORF_2 rf(1)" misc_binding 5339..5351 /dbxref="REBASE:SfiI" Marker 5395..5994 /gene="puro marker" Other Gene 6604..6656 /gene="delta_U3 other" Other Gene 6657..6837 /gene="HIV-1_5_LTR other" Rep_Origin 7344..7963 /gene="pBR322 origin" Promoter 8221..8270 /gene="NEOKAN prom" misc_binding 8328..8333 /dbxref="REBASE:BclI" ORF 8359..9153 /sequence="ORF_3 rf(1)" Marker 8362..9150 /gene="NTP_II marker" Rep_Origin 9258..9564 /gene="f1 origin" Other Gene 9619..9720 /gene="RITR other" Other Gene 9619..9720 /gene="LITR other" Other Gene 9800..9948 /gene="Encap other" BASE COUNT 2456 a 2702 c 2811 g 2228 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 aatggcttcg taccccggcc atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg 2521 cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt 2581 ccgcccggag cagaaaatgc ccacgctact gcgggtttat atagacggtc cccacgggat 2641 ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta 2701 cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat 2761 ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg cggcggtggt 2821 aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg ccgttctggc 2881 tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc tcaccctcat 2941 cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcggt accttatggg 3001 cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga ccttgcccgg 3061 caccaacatc gtgcttgggg cccttccgga ggacagacac atcgaccgcc tggccaaacg 3121 ccagcgcccc ggcgagcggc tggacctggc tatgctggct gcgattcgcc gcgtttacgg 3181 gctacttgcc aatacggtgc ggtatctgca gtgcggcggg tcgtggcggg aggactgggg 3241 acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca acgcgggccc 3301 acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt tgctggcccc 3361 caacggcgac ctgtataacg tgtttgcctg ggccttggac gtcttggcca aacgcctccg 3421 ttccatgcac gtctttatcc tggattacga ccaatcgccc gccggctgcc gggacgccct 3481 gctgcaactt acctccggga tggtccagac ccacgtcacc acccccggct ccataccgac 3541 gatatgcgac ctggcgcgca cgtttgcccg ggagatgggg gaggctaact gactcgagca 3601 attcgttaac ctcgagcggg atcaattccg ccccccccct aacgttactg gccgaagccg 3661 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 3721 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 3781 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 3841 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 3901 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 3961 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 4021 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 4081 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 4141 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggtga 4201 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 4261 taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc 4321 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 4381 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 4441 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 4501 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 4561 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 4621 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 4681 aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 4741 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 4801 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 4861 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taatgaatta 4921 attaagaatt atcaccgctt ctattcagcc agtaaggcct gtctttaatg gcctccggca 4981 tgagacactt tctagagtcg acgatatcgg gcccgcggcc gctggatcct ctagactgca 5041 gctcgagtac ccatacgacg tcccagacta cgcttgagtt taaacacgcg tggtgtggaa 5101 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 5161 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 5221 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 5281 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 5341 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc 5401 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc 5461 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac 5521 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5581 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5641 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5701 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg 5761 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 5821 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5881 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5941 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt 6001 tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 6421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6541 cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc 6601 ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct tgtactgggt 6661 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6721 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6781 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6841 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6901 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7021 atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa tgaaatggca 7081 ttatgggtat tatgggtctg cattaatgaa tcggccaacg gatgtcggtg tgaaataccg 7141 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7981 gctcagtgga acgaaaactc acgttaaggg attttggcca tgagattatc aaaaaggatc 8041 ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg 8101 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta 8161 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa 8221 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg 8281 atggctttct tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca 8341 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 8401 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 8461 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 8521 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 8581 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 8641 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 8701 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 8761 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 8821 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 8881 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 8941 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 9001 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 9061 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 9121 gccttctatc gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag 9181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 9241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 9301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 9361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 9421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 9481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 9541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 9601 gaattaattc ttaattaaca tcatcaataa tatacctttt ttggattgaa gccaatatga 9661 taatgagggg gtggagtttg tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag 9721 tagtgtggcg gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg 9781 caaaagtgac gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt 9841 ttaggcggat gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg 9901 aaaactgaat aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatac 9961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10021 cacatttccc cgaaaagtgc cacctgacgt aactataacg gtcctaaggt agcgaaaatg 10081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 10141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgat //
                        
***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60 GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC HIV-1_5_LTR other(109,289)>>> | 61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120 GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC 121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180 AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA 181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240 GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA 241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300 TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT 301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360 TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC HIV-1_psi_pack other(400,444)>>> | 361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420 GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT 421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480 GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT 481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540 AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT 541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600 TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC 601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660 TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA 661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720 GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT 721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780 ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT 781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840 TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA 841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900 ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG RRE reg(951,1184)>>> | 901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960 TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC ORF_1 rf(1)(994,1695)>>> | 961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020 AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC 1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080 CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA 1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140 TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT 1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200 TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG 1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260 AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA 1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320 GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG 1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380 TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT 1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440 CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC 1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500 GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA 1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560 AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC 1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620 TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT 1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680 CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA 1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740 CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA 1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800 TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT 1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860 TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC 1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920 GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT 1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980 CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG NdeI | 1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040 GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG 2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100 CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC 2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160 GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT 2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220 TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT CMV prom(2279,2360)>>> | 2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280 TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC 2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340 GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG 2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400 CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT tetO reg(2416,2455)>>> | 2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460 CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC ORF_4 rf(2)(2462,3592)>>> | 2461 aatggcttcgtaccccggccatcaacacgcgtctgcgttcgaccaggctgcgcgttctcg 2520 TTACCGAAGCATGGGGCCGGTAGTTGTGCGCAGACGCAAGCTGGTCCGACGCGCAAGAGC 2521 cggccatagcaaccgacgtacggcgttgcgccctcgccggcagcaagaagccacggaagt 2580 GCCGGTATCGTTGGCTGCATGCCGCAACGCGGGAGCGGCCGTCGTTCTTCGGTGCCTTCA 2581 ccgcccggagcagaaaatgcccacgctactgcgggtttatatagacggtccccacgggat 2640 GGCGGGCCTCGTCTTTTACGGGTGCGATGACGCCCAAATATATCTGCCAGGGGTGCCCTA 2641 ggggaaaaccaccaccacgcaactgctggtggccctgggttcgcgcgacgatatcgtcta 2700 CCCCTTTTGGTGGTGGTGCGTTGACGACCACCGGGACCCAAGCGCGCTGCTATAGCAGAT 2701 cgtacccgagccgatgacttactggcgggtgctgggggcttccgagacaatcgcgaacat 2760 GCATGGGCTCGGCTACTGAATGACCGCCCACGACCCCCGAAGGCTCTGTTAGCGCTTGTA 2761 ctacaccacacaacaccgcctcgaccagggtgagatatcggccggggacgcggcggtggt 2820 GATGTGGTGTGTTGTGGCGGAGCTGGTCCCACTCTATAGCCGGCCCCTGCGCCGCCACCA 2821 aatgacaagcgcccagataacaatgggcatgccttatgccgtgaccgacgccgttctggc 2880 TTACTGTTCGCGGGTCTATTGTTACCCGTACGGAATACGGCACTGGCTGCGGCAAGACCG 2881 tcctcatatcgggggggaggctgggagctcacatgccccgcccccggccctcaccctcat 2940 AGGAGTATAGCCCCCCCTCCGACCCTCGAGTGTACGGGGCGGGGGCCGGGAGTGGGAGTA 2941 cttcgaccgccatcccatcgccgccctcctgtgctacccggccgcgcggtaccttatggg 3000 GAAGCTGGCGGTAGGGTAGCGGCGGGAGGACACGATGGGCCGGCGCGCCATGGAATACCC 3001 cagcatgaccccccaggccgtgctggcgttcgtggccctcatcccgccgaccttgcccgg 3060 GTCGTACTGGGGGGTCCGGCACGACCGCAAGCACCGGGAGTAGGGCGGCTGGAACGGGCC 3061 caccaacatcgtgcttggggcccttccggaggacagacacatcgaccgcctggccaaacg 3120 GTGGTTGTAGCACGAACCCCGGGAAGGCCTCCTGTCTGTGTAGCTGGCGGACCGGTTTGC 3121 ccagcgccccggcgagcggctggacctggctatgctggctgcgattcgccgcgtttacgg 3180 GGTCGCGGGGCCGCTCGCCGACCTGGACCGATACGACCGACGCTAAGCGGCGCAAATGCC 3181 gctacttgccaatacggtgcggtatctgcagtgcggcgggtcgtggcgggaggactgggg 3240 CGATGAACGGTTATGCCACGCCATAGACGTCACGCCGCCCAGCACCGCCCTCCTGACCCC 3241 acagctttcggggacggccgtgccgccccagggtgccgagccccagagcaacgcgggccc 3300 TGTCGAAAGCCCCTGCCGGCACGGCGGGGTCCCACGGCTCGGGGTCTCGTTGCGCCCGGG 3301 acgaccccatatcggggacacgttatttaccctgtttcgggcccccgagttgctggcccc 3360 TGCTGGGGTATAGCCCCTGTGCAATAAATGGGACAAAGCCCGGGGGCTCAACGACCGGGG 3361 caacggcgacctgtataacgtgtttgcctgggccttggacgtcttggccaaacgcctccg 3420 GTTGCCGCTGGACATATTGCACAAACGGACCCGGAACCTGCAGAACCGGTTTGCGGAGGC 3421 ttccatgcacgtctttatcctggattacgaccaatcgcccgccggctgccgggacgccct 3480 AAGGTACGTGCAGAAATAGGACCTAATGCTGGTTAGCGGGCGGCCGACGGCCCTGCGGGA 3481 gctgcaacttacctccgggatggtccagacccacgtcaccacccccggctccataccgac 3540 CGACGTTGAATGGAGGCCCTACCAGGTCTGGGTGCAGTGGTGGGGGCCGAGGTATGGCTG SmaI | XmaI | | 3541 gatatgcgacctggcgcgcacgtttgcccgggagatgggggaggctaactgactcgagca 3600 CTATACGCTGGACCGCGCGTGCAAACGGGCCCTCTACCCCCTCCGATTGACTGAGCTCGT HpaI IRES reg(3631,4187)>>> | | 3601 attcgttaacctcgagcgggatcaattccgccccccccctaacgttactggccgaagccg 3660 TAAGCAATTGGAGCTCGCCCTAGTTAAGGCGGGGGGGGGATTGCAATGACCGGCTTCGGC ires_emcv reg(3694,4187)>>> | 3661 cttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttt 3720 GAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAA 3721 tggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtct 3780 ACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGA 3781 ttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctct 3840 AAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGA 3841 ggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaacccccc 3900 CCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGG 3901 acctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggc 3960 TGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCG 3961 ggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctc 4020 CCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAG 4021 ctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatc 4080 GAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAG 4081 tgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtct 4140 ACTAGACCCCGGAGCCACGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGA ORF_5 rf(3)(4194,4913)>>> | EGFP reporter(4194,4910)>>> | 4141 aggccccccgaaccacggggacgtggttttcctttgaaaaacacgataataccatggtga 4200 TCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTATTATGGTACCACT 4201 gcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacg 4260 CGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGC 4261 taaacggccacaagttcagcgtgtctggcgagggcgagggcgatgccacctacggcaagc 4320 ATTTGCCGGTGTTCAAGTCGCACAGACCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCG 4321 tgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtga 4380 ACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACT 4381 ccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacg 4440 GGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGC 4441 acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagg 4500 TGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCC 4501 acgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaacc 4560 TGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGG 4561 gcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgg 4620 CGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACC 4621 agtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatca 4680 TCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGT 4681 aggcgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccact 4740 TCCGCTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGA 4741 accagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctga 4800 TGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACT 4801 gcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg 4860 CGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACC 4861 agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatgaatta 4920 TCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTACTTAAT StuI | 4921 attaagaattatcaccgcttctattcagccagtaaggcctgtctttaatggcctccggca 4980 TAATTCTTAATAGTGGCGAAGATAAGTCGGTCATTCCGGACAGAAATTACCGGAGGCCGT NotI BamHI | | 4981 tgagacactttctagagtcgacgatatcgggcccgcggccgctggatcctctagactgca 5040 ACTCTGTGAAAGATCTCAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGT SV40 prom(5092,5360)>>> | 5041 gctcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaa 5100 CGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTT 5101 agtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaa 5160 TCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTT 5161 ccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca 5220 GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGT SV40 origin(5259,5336)>>> | 5221 attagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca 5280 TAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGT ORF_2 rf(1)(5326,5994)>>> | 5281 gttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgagg 5340 CAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCC SfiI puro marker(5395,5994)>>> | | 5341 ccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgacc 5400 GGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGG 5401 gagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcacc 5460 CTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGG 5461 ctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccac 5520 GAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTG 5521 atcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggc 5580 TAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCG 5581 aaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtc 5640 TTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAG 5641 gaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccgg 5700 CTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCC 5701 ctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcg 5760 GACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGC 5761 tggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgcc 5820 ACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGG 5821 gtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacc 5880 CAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGG 5881 tccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtc 5940 AGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAG 5941 gaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgt 6000 CTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCA 6001 tccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactat 6060 AGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATA 6061 gttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgct 6120 CAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGA 6121 tcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgag 6180 AGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTC 6181 gagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacc 6240 CTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGG 6241 cccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttcccc 6300 GGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGG 6301 ctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggct 6360 GAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGA 6361 cggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatgg 6420 GCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACC 6421 ctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcg 6480 GACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGC 6481 gccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccg 6540 CGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGC 6541 cgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtcc 6600 GCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGG delta_U3 other(6604,6656)>>> HIV-1_5_LTR other(6657,6837)>>> | | 6601 ggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggt 6660 CCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCA 6661 ctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgc 6720 GAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACG 6721 ttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtg 6780 AATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACAC 6781 actctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagta 6840 TGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCAT 6841 gtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagaga 6900 CATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCT 6901 gtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaa 6960 CACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTT 6961 atttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatca 7020 TAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGT 7021 atgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggca 7080 TACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGT 7081 ttatgggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaaataccg 7140 AATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGC 7141 cacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgac 7200 GTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTG 7201 tcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaata 7260 AGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTAT 7261 cggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa 7320 GCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTT pBR322 origin(7344,7963)<<< | 7321 aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct 7380 TTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGA 7381 gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataa 7440 CTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATT 7441 agataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg 7500 TCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGC 7501 cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctca 7560 GAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGT 7561 cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaa 7620 GCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTT 7621 ccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccg 7680 GGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGC 7681 gtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgagg 7740 CATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCC 7741 tatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagg 7800 ATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCC 7801 acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagc 7860 TGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCG 7861 tcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcag 7920 AGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTC 7921 attacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac 7980 TAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTG 7981 gctcagtggaacgaaaactcacgttaagggattttggccatgagattatcaaaaaggatc 8040 CGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAG 8041 ttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatg 8100 AAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTAC 8101 aatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggta 8160 TTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCAT 8161 gcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaa 8220 CGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTT NEOKAN prom(8221,8270)>>> | 8221 ccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactgg 8280 GGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACC BclI | 8281 atggctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagaca 8340 TACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGT NTP_II marker(8362,9150)>>> | ORF_3 rf(1)(8359,9153)>>> | | 8341 ggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgct 8400 CCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGA 8401 tgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgcc 8460 ACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGG 8461 gccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtcc 8520 CGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGG 8521 ggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggc 8580 CCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCG 8581 gttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattg 8640 CAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAAC 8641 ggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcc 8700 CCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGG 8701 atcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgac 8760 TAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTG 8761 caccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgat 8820 GTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTA 8821 caggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctc 8880 GTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAG 8881 aaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccg 8940 TTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGC 8941 aatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtg 9000 TTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACAC 9001 gcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggc 9060 CGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCG 9061 gaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatc 9120 CTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAG 9121 gccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcag 9180 CGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTC 9181 ctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagac 9240 GAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTG f1 origin(9258,9564)<<< | 9241 cgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgga 9300 GCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCT 9301 ctccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatc 9360 GAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAG 9361 accctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagg 9420 TGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCC 9421 gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaa 9480 CTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTT 9481 gaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaac 9540 CTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTG 9541 caccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 9600 GTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG RITR other(9619,9720)<<< | LITR other(9619,9720)>>> | 9601 gaattaattcttaattaacatcatcaataatataccttttttggattgaagccaatatga 9660 CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGTTATACT 9661 taatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtag 9720 ATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATC 9721 tagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtgg 9780 ATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACC Encap other(9800,9948)>>> | 9781 caaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggtt 9840 GTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAA 9841 ttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcggg 9900 AATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCC 9901 aaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatac 9960 TTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGCATTATG 9961 tctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcg 10020 AGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGC 10021 cacatttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcgaaaatg 10080 GTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGCTTTTAC 10081 tagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgcc 10140 ATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGG 10141 ttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgat 10197 AATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTA
                            LOCUS dna 10197 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" Regulatory_Seq 2416..2455 /gene="tetO reg" ORF 2462..3592 /sequence="ORF_4 rf(2)" misc_binding 3567..3572 /dbxref="REBASE:XmaI" misc_binding 3567..3572 /dbxref="REBASE:SmaI" misc_binding 3605..3610 /dbxref="REBASE:HpaI" Regulatory_Seq 3631..4187 /gene="IRES reg" Regulatory_Seq 3694..4187 /gene="ires_emcv reg" Reporter 4194..4910 /gene="EGFP reporter" ORF 4194..4913 /sequence="ORF_5 rf(3)" misc_binding 4955..4960 /dbxref="REBASE:StuI" misc_binding 5015..5022 /dbxref="REBASE:NotI" misc_binding 5024..5029 /dbxref="REBASE:BamHI" Promoter 5092..5360 /gene="SV40 prom" Rep_Origin 5259..5336 /gene="SV40 origin" ORF 5326..5994 /sequence="ORF_2 rf(1)" misc_binding 5339..5351 /dbxref="REBASE:SfiI" Marker 5395..5994 /gene="puro marker" Other Gene 6604..6656 /gene="delta_U3 other" Other Gene 6657..6837 /gene="HIV-1_5_LTR other" Rep_Origin 7344..7963 /gene="pBR322 origin" Promoter 8221..8270 /gene="NEOKAN prom" misc_binding 8328..8333 /dbxref="REBASE:BclI" ORF 8359..9153 /sequence="ORF_3 rf(1)" Marker 8362..9150 /gene="NTP_II marker" Rep_Origin 9258..9564 /gene="f1 origin" Other Gene 9619..9720 /gene="RITR other" Other Gene 9619..9720 /gene="LITR other" Other Gene 9800..9948 /gene="Encap other" BASE COUNT 2456 a 2702 c 2811 g 2228 t 0 others