pLenti-III-HPV-16-E6E7-Puro
| Vector Name | pLenti-III-HPV-16-E6E7-Puro |
| VectorType | Lentivector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV Sequencing Primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | DEFINITION Human papillomavirus type 16 isolate HPV16-32 E6 protein (E6) gene, complete cds. ACCESSION EU880266 DEFINITION Human papillomavirus type 16 isolate LL31 E7 protein gene, complete cds. ACCESSION JX412167 Full E6/E7 Insert sequence: Atgcaccaaaagagaactgcaatgtttcaggacccacaggagcgacccagaaagttaccacagttatgcacagagc tgcaaacaactatacatgatataatattagaatgtgtgtactgcaagcaacagttactgcgacgtgaggtatatgacttt gcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgataaatgtttaaagttttattctaa aattagtgagtatagacattattgttatagtttgtatggaacaacattagaacagcaatacaacaaaccgttgtgtgatt tgttaattaggtgtattaactgtcaaaagccactgtgtcctgaagaaaagcaaagacatctggacaaaaagcaaagattc cataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaacacgtagagaaacccagctgta atcatgcatggagatacacctacattgcatgaatatatgttagatttgcaaccagagacaactgatctctactgttatga gcaattaaatgacagctcagaggaggaggatgaaatagatggtccagctggacaagcagaaccggacagagcccattaca atattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcacacacgtagacattcgtactttg gaagacctgttaatgggcacactaggaattgtgtgccccatctgttctcagaaaccataa |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgt
agtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttgg
tgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt
aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaat
acggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt
agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta
gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggaga
gaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctga
catggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct
ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcc
ttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgt
ggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc
ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctag
aaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggcc
agggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc
tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttaga
tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaa
gatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag
ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagag
aagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcacta
tgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg
agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgt
ggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgcctt
ggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac
aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaatt
agataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtag
gaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg
tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagaga
cagatccattcgattagtgaacggatctcgacggtatcgaaagcttgggattcgaatttaaaagaaaaggggggattggg
gggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa
aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccgcctggctgaccgcc
caacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaat
gggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc
aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgt
ttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatt
tccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca
actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgt
cagatcgcctggagacgccatccacgctgttttgacctccatagaagaaccgagtttaaactccctatcagtgatagaga
tctccctatcagtgatagagagctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc
aggtgaattcactagaGGATCCTTTTAtgcaccaaaagagaactgcaatgtttcaggacccacaggagcgacccagaaag
ttaccacagttatgcacagagctgcaaacaactatacatgatataatattagaatgtgtgtactgcaagcaacagttact
gcgacgtgaggtatatgactttgcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgata
aatgtttaaagttttattctaaaattagtgagtatagacattattgttatagtttgtatggaacaacattagaacagcaa
tacaacaaaccgttgtgtgatttgttaattaggtgtattaactgtcaaaagccactgtgtcctgaagaaaagcaaagaca
tctggacaaaaagcaaagattccataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaa
cacgtagagaaacccagctgtaatcatgcatggagatacacctacattgcatgaatatatgttagatttgcaaccagaga
caactgatctctactgttatgagcaattaaatgacagctcagaggaggaggatgaaatagatggtccagctggacaagca
gaaccggacagagcccattacaatattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcac
acacgtagacattcgtactttggaagacctgttaatgggcacactaggaattgtgtgccccatctgttctcagaaaccat
aaTCTACCATGGCTGATcctgcaggtaccggatccggtaccgagctcgaattcaagcttaagtttatcgggcccgcggcc
gctggatcctctagactgcagctcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaa
agtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtcccca
ggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccat
cccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgagg
ccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcg
cctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccaca
ccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggc
aaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgc
cgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgccgc
accggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgcc
gtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccc
cttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgca
agcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactat
gttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcatttt
ctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgca
ctgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttcccc
ctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaa
ttccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgt
ccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccg
cgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcac
tcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctgg
ctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtg
actctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttatt
attcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaa
ataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatca
atgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctg
cattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttc
cgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac
ggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaag
gccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcg
aaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgc
ttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcg
gtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaacta
tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggt
atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgct
ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttt
ttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg
ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat
taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggc
acctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccag
ccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagc
tagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaa
agccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaa
agagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaa
ttgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctg
atggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcag
gttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtg
ttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacga
ggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggg
actggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatg
gctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcg
agcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaac
tgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatc
atggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgtt
ggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccg
attcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcatt
ttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccag
tttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggccca
ctacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagccc
ccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctaggg
cgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattc
gccattcaggatcgaattaattcttaattaacatcatcaataatatacctt
LOCUS dna 9033 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
ORF 3149..3943
/sequence="ORF_2 rf(2)"
misc_binding 4011..4016
/dbxref="REBASE:ApaI"
misc_binding 4017..4024
/dbxref="REBASE:NotI"
misc_binding 4032..4037
/dbxref="REBASE:XbaI"
misc_binding 4044..4049
/dbxref="REBASE:XhoI"
Promoter 4094..4362
/gene="SV40 prom"
Rep_Origin 4261..4338
/gene="SV40 origin"
ORF 4328..4996
/sequence="ORF_3 rf(2)"
Marker 4397..4996
/gene="puro marker"
Other Gene 5606..5658
/gene="delta_U3 other"
Other Gene 5659..5839
/gene="HIV-1_5_LTR other"
Rep_Origin 6345..6964
/gene="pBR322 origin"
Promoter 7617..7666
/gene="NEOKAN prom"
misc_binding 7724..7729
/dbxref="REBASE:BclI"
ORF 7755..8549
/sequence="ORF_4 rf(3)"
Marker 7758..8546
/gene="NTP_II marker"
Rep_Origin 8654..8959
/gene="f1 origin"
BASE COUNT 2263 a 2271 c 2460 g 2039 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagactcg acgccaccat ggactggacc tggatcctgt tcctggtggc
3181 cgccgccaca cgggtgcaca gcttccagga cccccaggag agcggcagaa agctgcctca
3241 gctgtgtacc gagctgcaga ccaccatcca cgacatcatc ctggagtgtg tgtactgtaa
3301 gcagcagctg ctgaggagag aggtgtacga ccgggacctg tgtatcgtgt acagggacgg
3361 caatccctac gccgtgtgtg acaagtgcct gaagttctac agcaagatca gcgagtaccg
3421 gcactactgc tacagcctgt acggcaccac cctggagcag cagtacaaca agcccctgtg
3481 tgacctgctg atccggtgta tcaactgcca gaagcccctg cagagacacc tggacaagaa
3541 gcagcggttc cacaacatca ggggcagatg gaccggcaga tgtatgagct gctgccggag
3601 cagcagaacc agaagggaga cccagctgag aggccggaag agaagaagcc acggcgatac
3661 ccccaccctg cacgagtaca tgctggacct gcagcctgag accaccgatc tgtacggcta
3721 cggccagctg aatgacagca gcgaggagga ggatgagatc gacggccctg ccggccaggc
3781 cgagcccgac agagcccact acaacatcgt gaccttttgc tgtaagtgtg acagcaccct
3841 gagactgtgc gtgcagagca cccacgtgga catcagaacc ctggaggatc tgctgatggg
3901 caccctgggc atcgtgtgtc ccatctgctc ccagaaaccc tgatctacca tggctgatac
3961 ctgcaggtac cggatccggt accgagctcg aattcaagct taagtttatc gggcccgcgg
4021 ccgctggatc ctctagactg cagctcgagt acccatacga cgtcccagac tacgcttgag
4081 tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4141 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
4201 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
4261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
4321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
4381 ggcttttttg gaggccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
4441 cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
4501 caccgtggac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
4561 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
4621 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
4681 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaagggc tcctggcgcc
4741 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
4801 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
4861 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
4921 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
4981 caagcccggt gcctgaacgc gttccggaaa tcaacctctg gattacaaaa tttgtgaaag
5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5401 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
5521 gccggctctg cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg gatctccctt
5581 tgggccgcct ccccgcctgt ccggatggaa gggctaattc actcccaacg aatacaagat
5641 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
5701 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
5761 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
5821 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
5881 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
5941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
6001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggcatctatg tcgggtgcgg
6061 agaaagaggt aatgaaatgg cattatgggt attatgggtc tgcattaatg aatcggccaa
6121 cgatcccggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6781 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgaaaa aggatcttca
7441 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
7501 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
7561 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
7621 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
7681 ctttctcgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
7741 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
7801 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
7861 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
7921 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
7981 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
8041 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
8101 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
8161 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
8221 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
8281 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
8341 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
8401 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
8461 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
8521 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca
8581 ttttttaacc aataggccga aatcggcaac atcccttata aatcaaaaga atagaccgcg
8641 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
8701 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
8761 aaatcaagtt ttttgcggtc gaggtgccgt aaagctctaa atcggaaccc taaagggagc
8821 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
8881 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
8941 acacccgcgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt
9001 aattcttaat taacatcatc aataatatac ctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI
| | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG
ORF_2 rf(2)(3149,3943)>>>
|
3121 aggtgaattcactagactcgacgccaccatggactggacctggatcctgttcctggtggc 3180
TCCACTTAAGTGATCTGAGCTGCGGTGGTACCTGACCTGGACCTAGGACAAGGACCACCG
3181 cgccgccacacgggtgcacagcttccaggacccccaggagagcggcagaaagctgcctca 3240
GCGGCGGTGTGCCCACGTGTCGAAGGTCCTGGGGGTCCTCTCGCCGTCTTTCGACGGAGT
3241 gctgtgtaccgagctgcagaccaccatccacgacatcatcctggagtgtgtgtactgtaa 3300
CGACACATGGCTCGACGTCTGGTGGTAGGTGCTGTAGTAGGACCTCACACACATGACATT
3301 gcagcagctgctgaggagagaggtgtacgaccgggacctgtgtatcgtgtacagggacgg 3360
CGTCGTCGACGACTCCTCTCTCCACATGCTGGCCCTGGACACATAGCACATGTCCCTGCC
3361 caatccctacgccgtgtgtgacaagtgcctgaagttctacagcaagatcagcgagtaccg 3420
GTTAGGGATGCGGCACACACTGTTCACGGACTTCAAGATGTCGTTCTAGTCGCTCATGGC
3421 gcactactgctacagcctgtacggcaccaccctggagcagcagtacaacaagcccctgtg 3480
CGTGATGACGATGTCGGACATGCCGTGGTGGGACCTCGTCGTCATGTTGTTCGGGGACAC
3481 tgacctgctgatccggtgtatcaactgccagaagcccctgcagagacacctggacaagaa 3540
ACTGGACGACTAGGCCACATAGTTGACGGTCTTCGGGGACGTCTCTGTGGACCTGTTCTT
3541 gcagcggttccacaacatcaggggcagatggaccggcagatgtatgagctgctgccggag 3600
CGTCGCCAAGGTGTTGTAGTCCCCGTCTACCTGGCCGTCTACATACTCGACGACGGCCTC
3601 cagcagaaccagaagggagacccagctgagaggccggaagagaagaagccacggcgatac 3660
GTCGTCTTGGTCTTCCCTCTGGGTCGACTCTCCGGCCTTCTCTTCTTCGGTGCCGCTATG
3661 ccccaccctgcacgagtacatgctggacctgcagcctgagaccaccgatctgtacggcta 3720
GGGGTGGGACGTGCTCATGTACGACCTGGACGTCGGACTCTGGTGGCTAGACATGCCGAT
3721 cggccagctgaatgacagcagcgaggaggaggatgagatcgacggccctgccggccaggc 3780
GCCGGTCGACTTACTGTCGTCGCTCCTCCTCCTACTCTAGCTGCCGGGACGGCCGGTCCG
3781 cgagcccgacagagcccactacaacatcgtgaccttttgctgtaagtgtgacagcaccct 3840
GCTCGGGCTGTCTCGGGTGATGTTGTAGCACTGGAAAACGACATTCACACTGTCGTGGGA
3841 gagactgtgcgtgcagagcacccacgtggacatcagaaccctggaggatctgctgatggg 3900
CTCTGACACGCACGTCTCGTGGGTGCACCTGTAGTCTTGGGACCTCCTAGACGACTACCC
3901 caccctgggcatcgtgtgtcccatctgctcccagaaaccctgaTCTACCATGGCTGATAC 3960
GTGGGACCCGTAGCACACAGGGTAGACGAGGGTCTTTGGGACTAGATGGTACCGACTATG
NotI
|
ApaI
| |
3961 CTGCAGGTACCGGATCCGGTACCGAGCTCGAATTCaagcttaagtttatcgggcccgcgg 4020
GACGTCCATGGCCTAGGCCATGGCTCGAGCTTAAGTTCGAATTCAAATAGCCCGGGCGCC
XbaI XhoI
| |
4021 ccgctggatcctctagactgcagctcgagtacccatacgacgtcccagactacgcttgag 4080
GGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTC
SV40 prom(4094,4362)>>>
|
4081 tttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagc 4140
AAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCG
4141 atgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcaga 4200
TACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCT
4201 agtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgccc 4260
TCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGG
SV40 origin(4261,4338)>>>
|
4261 atcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttt 4320
TAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAA
ORF_3 rf(2)(4328,4996)>>>
|
4321 tttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgagga 4380
AAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCT
puro marker(4397,4996)>>>
|
4381 ggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacga 4440
CCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCT
4441 cgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgcca 4500
GCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGT
4501 caccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcac 4560
GTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTG
4561 gcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggt 4620
CGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCA
4621 ctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcat 4680
GACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTA
4681 ggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgcc 4740
CCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGG
4741 gcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccacca 4800
CGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGT
4801 gggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccgg 4860
CCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCC
4861 ggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcgg 4920
CCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCC
4921 cttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccg 4980
GAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGC
4981 caagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaag 5040
GTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTC
5041 attgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaat 5100
TAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTA
5101 gcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatc 5160
CGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAG
5161 ctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtg 5220
GACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACAC
5221 cactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcct 5280
GTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGA
5281 ttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcct 5340
AAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGA
5341 tgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggg 5400
ACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCC
5401 gaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggac 5460
CTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTG
5461 gtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgct 5520
CAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGA
5521 gccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctt 5580
CGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAA
delta_U3 other(5606,5658)>>>
|
5581 tgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagat 5640
ACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTA
HIV-1_5_LTR other(5659,5839)>>>
|
5641 ctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctct 5700
GACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGA
5701 ggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagta 5760
CCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCAT
5761 gtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtca 5820
CACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGT
5821 gtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataactt 5880
CACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAA
5881 gcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttac 5940
CGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATG
5941 aaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagt 6000
TTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCA
6001 tgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcgg 6060
ACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCC
6061 agaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaa 6120
TCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTT
6121 cgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctct 6180
GCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGA
6181 tccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatca 6240
AGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGT
6241 gctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac 6300
CGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTG
pBR322 origin(6345,6964)<<<
|
6301 atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttt 6360
TACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAA
6361 ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtgg 6420
AAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACC
6421 cgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgc 6480
GCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCG
6481 tctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagc 6540
AGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCG
6541 gtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcc 6600
CACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGG
6601 aagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac 6660
TTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTG
6661 tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggt 6720
ATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCA
6721 aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcct 6780
TTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGA
6781 aactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacc 6840
TTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGG
6841 ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt 6900
AAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCA
6901 ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttg 6960
AAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAAC
6961 atcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtc 7020
TAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAG
7021 atgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaa 7080
TACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTT
7081 tcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgag 7140
AGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTC
7141 gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtg 7200
CGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCAC
7201 tagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcga 7260
ATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCT
7261 gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgag 7320
CTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTC
7321 cgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa 7380
GCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTT
7381 gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttca 7440
CGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGT
7441 cctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatg 7500
GGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTAC
7501 tcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagctt 7560
AGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAA
NEOKAN prom(7617,7666)>>>
|
7561 gcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccgg 7620
CGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCC
7621 aattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatgg 7680
TTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACC
BclI
|
7681 ctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggat 7740
GAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTA
NTP_II marker(7758,8546)>>>
|
ORF_4 rf(3)(7755,8549)>>>
| |
7741 gaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttggg 7800
CTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCC
7801 tggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccg 7860
ACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGC
7861 tgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtg 7920
ACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCAC
7921 ccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttc 7980
GGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAG
7981 cttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcg 8040
GAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGC
8041 aagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatca 8100
TTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGT
8101 tggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccacc 8160
ACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGG
8161 aagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagg 8220
TTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCC
8221 atgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaagg 8280
TACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCC
8281 cgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaata 8340
GCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTAT
8341 tcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcgg 8400
AGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCC
8401 accgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaat 8460
TGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTA
8461 gggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcct 8520
CCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGA
8521 tctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctca 8580
AGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGT
8581 ttttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcg 8640
AAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGC
f1 origin(8654,8959)<<<
|
8641 atagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactcc 8700
TATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGG
8701 aacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccc 8760
TTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGG
8761 aaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagc 8820
TTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCG
8821 ccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaa 8880
GGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTT
8881 gcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccacc 8940
CGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGG
8941 acacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaatt 9000
TGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAA
9001 aattcttaattaacatcatcaataatatacctt 9033
TTAAGAATTAATTGTAGTAGTTATTATATGGAA
.