pLenti-III-HPV-16-E6-E7-No Selection
| Vector Name | pLenti-III-HPV-16-E6-E7-No Selection |
| VectorType | Lentivector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV Sequencing Primer5`---CGC AAA TGG GCG GTA GGC GTG---3`Reverse Sequencing Primer5`---GAA AGC CAT ACG GGA AGC AAT AG---3` |
| Additional Information | DEFINITION Human papillomavirus type 16 isolate HPV16-32 E6 protein (E6) gene, complete cds. ACCESSION EU880266 DEFINITION Human papillomavirus type 16 isolate LL31 E7 protein gene, complete cds. ACCESSION JX412167Full E6/E7 Insert sequence: Atgcaccaaaagagaactgcaatgtttcaggacccacaggagcgacccagaaagttaccacagttatgcacagagc tgcaaacaactatacatgatataatattagaatgtgtgtactgcaagcaacagttactgcgacgtgaggtatatgacttt gcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgataaatgtttaaagttttattctaa aattagtgagtatagacattattgttatagtttgtatggaacaacattagaacagcaatacaacaaaccgttgtgtgatt tgttaattaggtgtattaactgtcaaaagccactgtgtcctgaagaaaagcaaagacatctggacaaaaagcaaagattc cataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaacacgtagagaaacccagctgta atcatgcatggagatacacctacattgcatgaatatatgttagatttgcaaccagagacaactgatctctactgttatga gcaattaaatgacagctcagaggaggaggatgaaatagatggtccagctggacaagcagaaccggacagagcccattaca atattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcacacacgtagacattcgtactttg gaagacctgttaatgggcacactaggaattgtgtgccccatctgttctcagaaaccataa |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc 3121 aggtgaattc actagaggat ccttttatgc accaaaagag aactgcaatg tttcaggacc 3181 cacaggagcg acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg 3241 atataatatt agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact 3301 ttgcttttcg ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata 3361 aatgtttaaa gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg 3421 gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta 3481 actgtcaaaa gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat 3541 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa 3601 cacgtagaga aacccagctg taatcatgca tggagataca cctacattgc atgaatatat 3661 gttagatttg caaccagaga caactgatct ctactgttat gagcaattaa atgacagctc 3721 agaggaggag gatgaaatag atggtccagc tggacaagca gaaccggaca gagcccatta 3781 caatattgta accttttgtt gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac 3841 acacgtagac attcgtactt tggaagacct gttaatgggc acactaggaa ttgtgtgccc 3901 catctgttct cagaaaccat aatctaccat ggctgatcct gcaggtaccg gatccggtac 3961 cgagctcgaa ttcaagctta agtttatcgg gcccgcggcc gctggatcct ctagactgca 4021 gctcgagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 4201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 4261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 4321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 4381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 4441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 4501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 4561 gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc 4621 cggatggaag ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg 4681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 4741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 4801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 4861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 4921 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 5041 aatgtatctt atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc 5101 attatgggta ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg 5161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 5221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 5581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 5641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 5701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 5761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 5821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 5881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 5941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6421 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga 6481 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga 6541 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat 6601 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct 6661 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct 6721 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg 6781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 6841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 6901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 6961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 7021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 7081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 7141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 7201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 7261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 7321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 7381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 7441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 7501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 7561 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 7621 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca 7681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 7741 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg 7801 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 7861 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 7921 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc 7981 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 8041 ataatatacc tt
LOCUS dna 8052 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" ORF 3929..4636 /sequence="ORF_2 rf(2)" misc_binding 3989..3994 /dbxref="REBASE:ApaI" misc_binding 3995..4002 /dbxref="REBASE:NotI" misc_binding 4010..4015 /dbxref="REBASE:XbaI" misc_binding 4022..4027 /dbxref="REBASE:XhoI" Other Gene 4625..4677 /gene="delta_U3 other" Other Gene 4678..4858 /gene="HIV-1_5_LTR other" Rep_Origin 5364..5983 /gene="pBR322 origin" Promoter 6636..6685 /gene="NEOKAN prom" misc_binding 6743..6748 /dbxref="REBASE:BclI" ORF 6774..7568 /sequence="ORF_3 rf(3)" Marker 6777..7565 /gene="NTP_II marker" Rep_Origin 7673..7978 /gene="f1 origin" BASE COUNT 2158 a 1817 c 2104 g 1973 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc 3121 aggtgaattc actagaggat ccttttatgc accaaaagag aactgcaatg tttcaggacc 3181 cacaggagcg acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg 3241 atataatatt agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact 3301 ttgcttttcg ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata 3361 aatgtttaaa gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg 3421 gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta 3481 actgtcaaaa gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat 3541 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa 3601 cacgtagaga aacccagctg taatcatgca tggagataca cctacattgc atgaatatat 3661 gttagatttg caaccagaga caactgatct ctactgttat gagcaattaa atgacagctc 3721 agaggaggag gatgaaatag atggtccagc tggacaagca gaaccggaca gagcccatta 3781 caatattgta accttttgtt gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac 3841 acacgtagac attcgtactt tggaagacct gttaatgggc acactaggaa ttgtgtgccc 3901 catctgttct cagaaaccat aatctaccat ggctgatcct gcaggtaccg gatccggtac 3961 cgagctcgaa ttcaagctta agtttatcgg gcccgcggcc gctggatcct ctagactgca 4021 gctcgagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 4201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 4261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 4321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 4381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 4441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 4501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 4561 gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc 4621 cggatggaag ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg 4681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 4741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 4801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 4861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 4921 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 5041 aatgtatctt atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc 5101 attatgggta ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg 5161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 5221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 5581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 5641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 5701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 5761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 5821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 5881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 5941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6421 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga 6481 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga 6541 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat 6601 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct 6661 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct 6721 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg 6781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 6841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 6901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 6961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 7021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 7081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 7141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 7201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 7261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 7321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 7381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 7441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 7501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 7561 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 7621 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca 7681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 7741 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg 7801 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 7861 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 7921 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc 7981 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 8041 ataatatacc tt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC SmaI | XmaI ClaI | | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG 3121 aggtgaattcactagaGGATCCTTTTAtgcaccaaaagagaactgcaatgtttcaggacc 3180 TCCACTTAAGTGATCTCCTAGGAAAATACGTGGTTTTCTCTTGACGTTACAAAGTCCTGG 3181 cacaggagcgacccagaaagttaccacagttatgcacagagctgcaaacaactatacatg 3240 GTGTCCTCGCTGGGTCTTTCAATGGTGTCAATACGTGTCTCGACGTTTGTTGATATGTAC 3241 atataatattagaatgtgtgtactgcaagcaacagttactgcgacgtgaggtatatgact 3300 TATATTATAATCTTACACACATGACGTTCGTTGTCAATGACGCTGCACTCCATATACTGA 3301 ttgcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgata 3360 AACGAAAAGCCCTAAATACGTATCATATATCTCTACCCTTAGGTATACGACATACACTAT 3361 aatgtttaaagttttattctaaaattagtgagtatagacattattgttatagtttgtatg 3420 TTACAAATTTCAAAATAAGATTTTAATCACTCATATCTGTAATAACAATATCAAACATAC 3421 gaacaacattagaacagcaatacaacaaaccgttgtgtgatttgttaattaggtgtatta 3480 CTTGTTGTAATCTTGTCGTTATGTTGTTTGGCAACACACTAAACAATTAATCCACATAAT 3481 actgtcaaaagccactgtgtcctgaagaaaagcaaagacatctggacaaaaagcaaagat 3540 TGACAGTTTTCGGTGACACAGGACTTCTTTTCGTTTCTGTAGACCTGTTTTTCGTTTCTA 3541 tccataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaa 3600 AGGTATTATATTCCCCAGCCACCTGGCCAGCTACATACAGAACAACGTCTAGTAGTTCTT 3601 cacgtagagaaacccagctgtaatcatgcatggagatacacctacattgcatgaatatat 3660 GTGCATCTCTTTGGGTCGACATTAGTACGTACCTCTATGTGGATGTAACGTACTTATATA 3661 gttagatttgcaaccagagacaactgatctctactgttatgagcaattaaatgacagctc 3720 CAATCTAAACGTTGGTCTCTGTTGACTAGAGATGACAATACTCGTTAATTTACTGTCGAG 3721 agaggaggaggatgaaatagatggtccagctggacaagcagaaccggacagagcccatta 3780 TCTCCTCCTCCTACTTTATCTACCAGGTCGACCTGTTCGTCTTGGCCTGTCTCGGGTAAT 3781 caatattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcac 3840 GTTATAACATTGGAAAACAACGTTCACACTGAGATGCGAAGCCAACACGCATGTTTCGTG 3841 acacgtagacattcgtactttggaagacctgttaatgggcacactaggaattgtgtgccc 3900 TGTGCATCTGTAAGCATGAAACCTTCTGGACAATTACCCGTGTGATCCTTAACACACGGG ORF_2 rf(2)(3929,4636)>>> | 3901 catctgttctcagaaaccataaTCTACCATGGCTGATcctgcaggtaccggatccggtac 3960 GTAGACAAGAGTCTTTGGTATTAGATGGTACCGACTAGGACGTCCATGGCCTAGGCCATG NotI | ApaI XbaI | | | 3961 cgagctcgaattcaagcttaagtttatcgggcccgcggccgctggatcctctagactgca 4020 GCTCGAGCTTAAGTTCGAATTCAAATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGT XhoI | 4021 gctcgagaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaacta 4080 CGAGCTCTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGAT 4081 tgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgc 4140 ACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACG 4141 ttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatga 4200 AAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACT 4201 ggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaac 4260 CCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTG 4261 ccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccc 4320 GGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGG 4321 cctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggc 4380 GGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCG 4381 tcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatg 4440 AGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTAC 4441 gctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttc 4500 CGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAG 4501 ggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttcc 4560 CCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGG 4561 gcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtc 4620 CGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAG delta_U3 other(4625,4677)>>> HIV-1_5_LTR other(4678,4858)>>> | | 4621 cggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactggg 4680 GCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCC 4681 tctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactg 4740 AGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGAC 4741 cttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgt 4800 GAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACA 4801 gactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagt 4860 CTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCA 4861 agtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagag 4920 TCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTC 4921 agtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcaca 4980 TCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGT 4981 aatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatc 5040 TTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAG 5041 aatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggc 5100 TTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCG 5101 attatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccg 5160 TAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGC 5161 cacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgac 5220 GTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTG 5221 tcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaata 5280 AGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTAT 5281 cggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa 5340 GCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTT pBR322 origin(5364,5983)<<< | 5341 aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct 5400 TTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGA 5401 gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataa 5460 CTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATT 5461 agataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg 5520 TCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGC 5521 cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctca 5580 GAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGT 5581 cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaa 5640 GCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTT 5641 ccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccg 5700 GGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGC 5701 gtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgagg 5760 CATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCC 5761 tatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagg 5820 ATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCC 5821 acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagc 5880 TGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCG 5881 tcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcag 5940 AGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTC 5941 attacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac 6000 TAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTG 6001 gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatc 6060 CGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAG 6061 ttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgag 6120 AAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTC 6121 taaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgt 6180 ATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACA 6181 ctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggag 6240 GATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTC 6241 ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctcca 6300 CCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGT 6301 gatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaact 6360 CTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGA 6361 ttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgcca 6420 AATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGT 6421 gttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtaga 6480 CAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCT 6481 aagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctgga 6540 TTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCT 6541 caagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgat 6600 GTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTA NEOKAN prom(6636,6685)>>> | 6601 agctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccct 6660 TCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGA 6661 ctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatct 6720 GACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGA NTP_II marker(6777,7565)>>> | BclI ORF_3 rf(3)(6774,7568)>>> | | | 6721 gatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattg 6780 CTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAAC 6781 aacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatg 6840 TTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATAC 6841 actgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcagg 6900 TGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCC 6901 ggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacg 6960 CCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGC 6961 aggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacg 7020 TCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGC 7021 ttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcc 7080 AACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGG 7081 tgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggc 7140 ACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCG 7141 tgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagc 7200 ACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCG 7201 gagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatc 7260 CTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAG 7261 aggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagg 7320 TCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCC 7321 atctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgct 7380 TAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGA 7381 tttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgt 7440 AAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCA 7441 tggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgc 7500 ACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACG 7501 tttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagt 7560 AAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCA 7561 tcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaa 7620 AGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTT f1 origin(7673,7978)<<< | 7621 atcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttcca 7680 TAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGT 7681 gtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaacc 7740 CAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGG 7741 gtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcg 7800 CAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGC 7801 aggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgg 7860 TCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCC 7861 ggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagg 7920 CCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCC 7921 gcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgc 7980 CGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCG 7981 cgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatca 8040 GCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGT 8041 ataatatacctt 8052 TATTATATGGAA
LOCUS dna 8052 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" ORF 3929..4636 /sequence="ORF_2 rf(2)" misc_binding 3989..3994 /dbxref="REBASE:ApaI" misc_binding 3995..4002 /dbxref="REBASE:NotI" misc_binding 4010..4015 /dbxref="REBASE:XbaI" misc_binding 4022..4027 /dbxref="REBASE:XhoI" Other Gene 4625..4677 /gene="delta_U3 other" Other Gene 4678..4858 /gene="HIV-1_5_LTR other" Rep_Origin 5364..5983 /gene="pBR322 origin" Promoter 6636..6685 /gene="NEOKAN prom" misc_binding 6743..6748 /dbxref="REBASE:BclI" ORF 6774..7568 /sequence="ORF_3 rf(3)" Marker 6777..7565 /gene="NTP_II marker" Rep_Origin 7673..7978 /gene="f1 origin" BASE COUNT 2158 a 1817 c 2104 g 1973 t 0 others