pLenti-III-HA
Vector Name | pLenti-III-HA |
VectorType | Lentiviral vector |
Antibiotic Information | Kanamycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt 2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2701 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2761 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2821 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2881 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta 3001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac 3061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 3121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 3181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 3241 tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag 3301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 3361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 3421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 3481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 3541 acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 3721 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 3781 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 3841 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 3901 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 3961 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 4021 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 4081 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 4141 ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta 4201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 4261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 4321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 4381 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 4441 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 4561 tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa 4621 atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa 4681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 4741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 4801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 4861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 4921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 4981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 5041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 5101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 5161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 5221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 5281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 5341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 5401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 5461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 5521 tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa 5581 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc 5641 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag 5701 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca 5761 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta 5821 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa 5881 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 5941 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 6001 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 6061 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 6121 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 6181 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 6241 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 6301 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 6361 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 6421 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 6481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 6541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 6601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 6661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta 6721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 6781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 6841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 6901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 6961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 7021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 7081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc 7141 aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca 7201 atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg 7261 acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg 7321 atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc 7381 gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt 7441 cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg 7501 taatactctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 7561 tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg 7621 aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 7681 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 7741 gat
LOCUS dna 7743 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" Regulatory_Seq 2416..2455 /gene="tetO reg" misc_binding 2462..2467 /dbxref="REBASE:SmaI" misc_binding 2462..2467 /dbxref="REBASE:XmaI" misc_binding 2468..2473 /dbxref="REBASE:ClaI" misc_binding 2492..2497 /dbxref="REBASE:KpnI" misc_binding 2519..2524 /dbxref="REBASE:EcoRI" misc_binding 2537..2542 /dbxref="REBASE:StuI" misc_binding 2543..2548 /dbxref="REBASE:HincII" misc_binding 2549..2554 /dbxref="REBASE:EcoRV" misc_binding 2555..2560 /dbxref="REBASE:ApaI" misc_binding 2561..2568 /dbxref="REBASE:NotI" misc_binding 2570..2575 /dbxref="REBASE:BamHI" misc_binding 2576..2581 /dbxref="REBASE:XbaI" misc_binding 2588..2593 /dbxref="REBASE:XhoI" Promoter 2638..2906 /gene="SV40 prom" Rep_Origin 2805..2882 /gene="SV40 origin" ORF 2872..3540 /sequence="ORF_2 rf(1)" misc_binding 2885..2897 /dbxref="REBASE:SfiI" Marker 2941..3540 /gene="puro marker" Other Gene 4150..4202 /gene="delta_U3 other" Other Gene 4203..4383 /gene="HIV-1_5_LTR other" Rep_Origin 4890..5509 /gene="pBR322 origin" Promoter 5767..5816 /gene="NEOKAN prom" misc_binding 5874..5879 /dbxref="REBASE:BclI" ORF 5905..6699 /sequence="ORF_3 rf(1)" Marker 5908..6696 /gene="NTP_II marker" Rep_Origin 6804..7110 /gene="f1 origin" Other Gene 7165..7266 /gene="RITR other" Other Gene 7165..7266 /gene="LITR other" Other Gene 7346..7494 /gene="Encap other" BASE COUNT 1937 a 1906 c 2120 g 1780 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt 2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2701 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2761 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2821 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2881 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta 3001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac 3061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 3121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 3181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 3241 tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag 3301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 3361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 3421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 3481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 3541 acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 3721 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 3781 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 3841 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 3901 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 3961 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 4021 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 4081 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 4141 ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta 4201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 4261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 4321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 4381 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 4441 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 4561 tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa 4621 atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa 4681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 4741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 4801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 4861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 4921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 4981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 5041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 5101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 5161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 5221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 5281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 5341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 5401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 5461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 5521 tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa 5581 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc 5641 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag 5701 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca 5761 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta 5821 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa 5881 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 5941 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 6001 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 6061 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 6121 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 6181 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 6241 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 6301 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 6361 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 6421 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 6481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 6541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 6601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 6661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta 6721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 6781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 6841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 6901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 6961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 7021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 7081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc 7141 aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca 7201 atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg 7261 acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg 7321 atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc 7381 gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt 7441 cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg 7501 taatactctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 7561 tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg 7621 aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 7681 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 7741 gat //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60 GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC HIV-1_5_LTR other(109,289)>>> | 61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120 GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC 121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180 AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA 181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240 GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA 241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300 TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT 301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360 TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC HIV-1_psi_pack other(400,444)>>> | 361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420 GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT 421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480 GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT NruI | 481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540 AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT 541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600 TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC 601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660 TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA 661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720 GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT 721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780 ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT 781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840 TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA 841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900 ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG RRE reg(951,1184)>>> | 901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960 TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC ORF_1 rf(1)(994,1695)>>> | 961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020 AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC 1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080 CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA 1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140 TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT 1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200 TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG 1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260 AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA 1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320 GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG 1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380 TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT 1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440 CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC 1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500 GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA 1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560 AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC 1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620 TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT 1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680 CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA 1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740 CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA 1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800 TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT 1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860 TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC 1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920 GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT 1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980 CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG NdeI | 1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040 GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG 2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100 CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC 2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160 GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT 2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220 TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT CMV prom(2279,2360)>>> | 2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280 TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC 2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340 GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG 2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400 CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT tetO reg(2416,2455)>>> | 2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460 CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC SmaI | XmaI ClaI KpnI | | | | 2461 ccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgcaggtga 2520 GGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACGTCCACT EcoRV NotI | | EcoRI StuI HincII ApaI BamHI XbaI | | | | | | | | 2521 attcactagtaccggtaggcctgtcgacgatatcgggcccgcggccgctggatcctctag 2580 TAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATC XhoI SV40 prom(2638,2906)>>> | | 2581 actgcagctcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggt 2640 TGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCA 2641 gtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagt 2700 CACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCA 2701 cagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgc 2760 GTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACG SV40 origin(2805,2882)>>> | 2761 atctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactc 2820 TAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAG ORF_2 rf(1)(2872,3540)>>> | 2821 cgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagagg 2880 GCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCC SfiI | 2881 ccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcc 2940 GGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGG puro marker(2941,3540)>>> | 2941 atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgta 3000 TACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCAT 3001 cgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggac 3060 GCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTG 3061 cgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgac 3120 GCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTG 3121 atcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggag 3180 TAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTC 3181 agcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggt 3240 TCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCA 3241 tcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggag 3300 AGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTC 3301 cccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggc 3360 GGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCG 3361 agcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctg 3420 TCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGAC 3421 gagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgcc 3480 CTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGG 3481 gacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctga 3540 CTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACT 3541 acgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattctt 3600 TGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAA 3601 aactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgct 3660 TTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGA 3661 attgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctt 3720 TAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAA 3721 tatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgac 3780 ATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTG 3781 gcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgct 3840 CGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGA 3841 ttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggaca 3900 AAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGT 3901 ggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtccttt 3960 CCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAA 3961 ccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtc 4020 GGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAG 4021 ccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcct 4080 GGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGA 4081 cttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgc 4140 GAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCG delta_U3 other(4150,4202)>>> | 4141 ctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgta 4200 GACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACAT HIV-1_5_LTR other(4203,4383)>>> | 4201 ctgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacc 4260 GACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGG 4261 cactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgt 4320 GTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACA 4321 tgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctcta 4380 ACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGAT 4381 gcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatat 4440 CGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATA 4441 cagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagca 4500 GTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGT 4501 tcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaac 4560 AGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTG 4561 tcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaa 4620 AGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTT 4621 atggcattatgggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaa 4680 TACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTT 4681 ataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc 4740 TATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAG 4741 actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg 4800 TGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGC 4801 gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc 4860 CATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCG pBR322 origin(4890,5509)<<< | 4861 cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc 4920 GTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCG 4921 ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga 4980 GGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCT 4981 ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc 5040 GATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGG 5041 ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat 5100 GACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTA 5101 agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg 5160 TCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACAC 5161 cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc 5220 GTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGG 5221 aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga 5280 TTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCT 5281 gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact 5340 CGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGA 5341 agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt 5400 TCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAA 5401 ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag 5460 CCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTC 5461 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg 5520 GTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCC 5521 tctgacgctcagtggaacgaaaactcacgttaagggattttggccatgagattatcaaaa 5580 AGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTT 5581 aggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccc 5640 TCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGG 5641 cggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaag 5700 GCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTC 5701 caggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagca 5760 GTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGT NEOKAN prom(5767,5816)>>> | 5761 agcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagta 5820 TCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCAT BclI | 5821 aactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaa 5880 TTGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTT NTP_II marker(5908,6696)>>> | ORF_3 rf(1)(5905,6699)>>> | | 5881 gagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccg 5940 CTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGC 5941 gccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctct 6000 CGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGA 6001 gatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgac 6060 CTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTG 6061 ctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacg 6120 GACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGC 6121 acgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctg 6180 TGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGAC 6181 ctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaa 6240 GATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTT 6241 gtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgccca 6300 CATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGT 6301 ttcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtctt 6360 AAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAA 6361 gtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcc 6420 CAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGG 6421 aggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgc 6480 TCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACG 6481 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctg 6540 AACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGAC 6541 ggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagctt 6600 CCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAA 6601 ggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcag 6660 CCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTC 6661 cgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgtta 6720 GCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAAT 6721 aatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaaga 6780 TTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCT f1 origin(6804,7110)<<< | 6781 atagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaa 6840 TATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTT 6841 cgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtga 6900 GCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACT 6901 accatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccc 6960 TGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGG 6961 taaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagga 7020 ATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCT 7021 agggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcg 7080 TCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGC 7081 cgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattc 7140 GCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAG LITR other(7165,7266)>>> | RITR other(7165,7266)<<< | 7141 aggatcgaattaattcttaattaacatcatcaataatataccttttttggattgaagcca 7200 TCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGT 7201 atatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtg 7260 TATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCAC 7261 acgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacgg 7320 TGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCC Encap other(7346,7494)>>> | 7321 atgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgc 7380 TACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCG 7381 gcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccatttt 7440 CGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAA 7441 cgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcg 7500 GCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGC 7501 taatactctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggt 7560 ATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCA 7561 tccgcgcacatttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcg 7620 AGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGC 7621 aaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaa 7680 TTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTT 7681 catgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtac 7740 GTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATG 7741 gat 7743 CTA
LOCUS dna 7743 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" Regulatory_Seq 2416..2455 /gene="tetO reg" misc_binding 2462..2467 /dbxref="REBASE:SmaI" misc_binding 2462..2467 /dbxref="REBASE:XmaI" misc_binding 2468..2473 /dbxref="REBASE:ClaI" misc_binding 2492..2497 /dbxref="REBASE:KpnI" misc_binding 2519..2524 /dbxref="REBASE:EcoRI" misc_binding 2537..2542 /dbxref="REBASE:StuI" misc_binding 2543..2548 /dbxref="REBASE:HincII" misc_binding 2549..2554 /dbxref="REBASE:EcoRV" misc_binding 2555..2560 /dbxref="REBASE:ApaI" misc_binding 2561..2568 /dbxref="REBASE:NotI" misc_binding 2570..2575 /dbxref="REBASE:BamHI" misc_binding 2576..2581 /dbxref="REBASE:XbaI" misc_binding 2588..2593 /dbxref="REBASE:XhoI" Promoter 2638..2906 /gene="SV40 prom" Rep_Origin 2805..2882 /gene="SV40 origin" ORF 2872..3540 /sequence="ORF_2 rf(1)" misc_binding 2885..2897 /dbxref="REBASE:SfiI" Marker 2941..3540 /gene="puro marker" Other Gene 4150..4202 /gene="delta_U3 other" Other Gene 4203..4383 /gene="HIV-1_5_LTR other" Rep_Origin 4890..5509 /gene="pBR322 origin" Promoter 5767..5816 /gene="NEOKAN prom" misc_binding 5874..5879 /dbxref="REBASE:BclI" ORF 5905..6699 /sequence="ORF_3 rf(1)" Marker 5908..6696 /gene="NTP_II marker" Rep_Origin 6804..7110 /gene="f1 origin" Other Gene 7165..7266 /gene="RITR other" Other Gene 7165..7266 /gene="LITR other" Other Gene 7346..7494 /gene="Encap other" BASE COUNT 1937 a 1906 c 2120 g 1780 t 0 others