pLenti-III-GFP-C
| Vector Name | pLenti-III-GFP-C |
| VectorType | Lentiviral vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctagggttcc gcgttacata 2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 2641 atgggacttt cctacttggc agtacatcta cgtttagtca tcgctattac catggtgatg 2701 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 2761 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 2821 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 2881 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 2941 tgttttgacc tccatagaag aaccgagttt aaactcccta tcagtgatag agatctccct 3001 atcagtgata gagagctagc cccgggatcg atcaattgag tacttacgta ggtaccccag 3061 tgtggtggcc tgcaggtgaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg 3121 cggccgctgg atcctctaga aaactcgaga tggtgagcaa gggcgaggag ctgttcaccg 3181 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 3241 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 3301 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 3361 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 3421 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 3481 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 3541 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 3601 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 3661 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 3721 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 3781 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3841 ctctcggcat ggacgagctg tacaagctgc aggtcgagta cccatacgac gtcccagact 3901 acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt 3961 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 4081 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 4201 tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac 4261 ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 4321 cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 4381 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 4441 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 4501 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct 4561 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 4621 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 4681 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 4741 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 4801 catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat 4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 4981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5221 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5341 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg 5401 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga 5461 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 5521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5641 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5701 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt 5881 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga 5941 atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 6001 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6841 gattttggcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 6901 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 6961 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 7021 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 7081 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg 7141 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 7201 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 7261 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 7321 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 7381 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 7441 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 7501 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 7561 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 7621 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 7681 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 7741 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 7801 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 7861 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 7921 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 7981 ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 8041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 8101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 8161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 8221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 8281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 8341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 8401 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 8461 atatacctt
FEATURES Location/Qualifiers source 1..8469 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 668..848 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 895..1020 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1510..1743 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2390..2694 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2695..2898 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2975..2993 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2996..3014 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 3150..3866 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 3150 .. 3152 / #05fd14 2: 3153 .. 3155 / #05fd14 / 1a 3: 3156 .. 3866 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3879..3905 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 4090..4225 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 4226..4825 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4838..5425 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(5309..5320) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 5308" /translation="IEGR" misc_feature 5435..5668 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 5488..5668 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5740..5861 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(6190..6778) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 7190..7984 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctagggttcc gcgttacata 2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 2641 atgggacttt cctacttggc agtacatcta cgtttagtca tcgctattac catggtgatg 2701 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 2761 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 2821 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 2881 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 2941 tgttttgacc tccatagaag aaccgagttt aaactcccta tcagtgatag agatctccct 3001 atcagtgata gagagctagc cccgggatcg atcaattgag tacttacgta ggtaccccag 3061 tgtggtggcc tgcaggtgaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg 3121 cggccgctgg atcctctaga aaactcgaga tggtgagcaa gggcgaggag ctgttcaccg 3181 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 3241 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 3301 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 3361 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 3421 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 3481 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 3541 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 3601 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 3661 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 3721 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 3781 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3841 ctctcggcat ggacgagctg tacaagctgc aggtcgagta cccatacgac gtcccagact 3901 acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt 3961 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 4081 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 4201 tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac 4261 ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 4321 cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 4381 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 4441 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 4501 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct 4561 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 4621 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 4681 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 4741 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 4801 catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat 4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 4981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5221 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5341 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg 5401 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga 5461 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 5521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5641 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5701 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt 5881 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga 5941 atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 6001 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6841 gattttggcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 6901 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 6961 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 7021 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 7081 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg 7141 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 7201 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 7261 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 7321 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 7381 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 7441 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 7501 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 7561 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 7621 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 7681 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 7741 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 7801 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 7861 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 7921 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 7981 ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 8041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 8101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 8161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 8221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 8281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 8341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 8401 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 8461 atatacctt
FEATURES Location/Qualifiers source 1..8469 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 668..848 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 895..1020 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1510..1743 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2390..2694 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2695..2898 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2975..2993 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2996..3014 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 3150..3866 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 3150 .. 3152 / #05fd14 2: 3153 .. 3155 / #05fd14 / 1a 3: 3156 .. 3866 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3879..3905 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 4090..4225 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 4226..4825 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4838..5425 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(5309..5320) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 5308" /translation="IEGR" misc_feature 5435..5668 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 5488..5668 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5740..5861 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(6190..6778) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 7190..7984 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"