pLenti-III-EF1alpha-GFP
| Vector Name | pLenti-III-EF1alpha-GFP |
| VectorType | Lentivirus |
| Antibiotic Information | Puromycin |
| Sequencing Primers | EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagcc gccaccatgg tgagcaaggg
3661 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
3721 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
3781 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
3841 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
3901 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
3961 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
4021 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
4081 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
4141 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
4201 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
4261 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
4321 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggta ccccagtgtg
4381 gtggcctgca ggtgaattca ctagtaccgg taggcctgtc gacgatatcg ggcccgcggc
4441 cgctggatcc tctagactgc agctcgagta cccatacgac gtcccagact acgcttgagt
4501 ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4561 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4621 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4681 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4741 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
4801 gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
4861 gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
4921 accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
4981 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
5041 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
5101 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct cctggcgccg
5161 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
5221 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
5281 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
5341 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
5401 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga
5461 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
5521 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
5581 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
5641 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
5701 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
5761 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
5821 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
5881 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
5941 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt
6001 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc
6061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
6121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
6181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
6241 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
6301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga
6481 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac
6541 gatcccggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6601 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6661 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6721 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6781 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6841 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6901 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6961 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
7021 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
7081 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
7141 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
7201 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
7261 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7321 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7381 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
7441 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
7501 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
7561 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
7621 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
7681 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
7741 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
7801 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac
7861 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
7921 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
7981 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
8041 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
8101 tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg
8161 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
8221 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
8281 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
8341 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
8401 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
8461 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
8521 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
8581 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
8641 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
8701 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
8761 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
8821 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
8881 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
8941 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat
9001 tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa tagaccgcga
9061 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
9121 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca
9181 aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc
9241 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
9301 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
9361 cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gatcgaatta
9421 attcttaatt aacatcatca ataatatacc tt
LOCUS dna 9452 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_6 rf(5)"
Promoter 2444..3621
/gene="EF1a prom"
ORF 3635..4561
/sequence="ORF_5 rf(5)"
Reporter 3647..4363
/gene="GFP reporter"
ORF 3647..4366
/sequence="ORF_3 rf(2)"
misc_binding 4367..4372
/dbxref="REBASE:KpnI"
misc_binding 4394..4399
/dbxref="REBASE:EcoRI"
misc_binding 4424..4429
/dbxref="REBASE:EcoRV"
misc_binding 4436..4443
/dbxref="REBASE:NotI"
misc_binding 4445..4450
/dbxref="REBASE:BamHI"
misc_binding 4451..4456
/dbxref="REBASE:XbaI"
Promoter 4513..4781
/gene="SV40 prom"
Rep_Origin 4680..4757
/gene="SV40 origin"
ORF 4747..5415
/sequence="ORF_2 rf(1)"
misc_binding 4760..4772
/dbxref="REBASE:SfiI"
Marker 4816..5415
/gene="puro marker"
Other Gene 6025..6077
/gene="delta_U3 other"
Other Gene 6078..6258
/gene="HIV-1_5_LTR other"
Rep_Origin 6764..7383
/gene="pBR322 origin"
Promoter 8036..8085
/gene="NEOKAN prom"
misc_binding 8143..8148
/dbxref="REBASE:BclI"
ORF 8174..8968
/sequence="ORF_4 rf(2)"
Marker 8177..8965
/gene="NTP_II marker"
Rep_Origin 9073..9378
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
ORF_6 rf(5)(2381,3361)<<<
|
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
EF1a prom(2444,3621)>>>
|
2401 aattcaaaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtca 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT
2461 gtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattg 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC
2521 aaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggct 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA
2581 ccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgt 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA
2641 tctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgg 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC
2701 gcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcag 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC
2761 tacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgc 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG
2821 gcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgc 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG
2881 cgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagcc 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG
2941 atttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaat 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA
3001 gcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcc 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG
3061 cgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatc 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG
3121 ggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgt 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA
3181 atcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaaga 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT
3241 tggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagag 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC
3301 cgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttca 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT
3361 tgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttgg 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC
3421 agtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgag 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC
3481 tgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcc 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG
3541 ctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttttttt 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA
ORF_3 rf(2)(3647,4366)>>>
|
GFP reporter(3647,4363)>>>
|
3601 cttccatttcaggtgtcgtgaggaatttcgactgctagccgccaccatggtgagcaaggg 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCGGCGGTGGTACCACTCGTTCCC
3661 cgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacgg 3720
GCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCC
3721 ccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccct 3780
GGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGA
3781 gaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccct 3840
CTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGA
3841 gacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttctt 3900
CTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAA
3901 caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg 3960
GTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCC
3961 caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcga 4020
GTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCT
4021 gctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaa 4080
CGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTT
4081 ctacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaa 4140
GATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTT
4141 cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca 4200
GAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGT
4201 gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccca 4260
CTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGT
4261 gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgt 4320
CAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCA
KpnI
|
4321 gaccgccgccgggatcactctcggcatggacgagctgtacaagtaaggtaccccagtgtg 4380
CTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTCCATGGGGTCACAC
EcoRI EcoRV NotI
| | |
4381 gtggcctgcaggtgaattcactagtaccggtaggcctgtcgacgatatcgggcccgcggc 4440
CACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCG
BamHI XbaI
| |
4441 cgctggatcctctagactgcagctcgagtacccatacgacgtcccagactacgcttgagt 4500
GCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCA
SV40 prom(4513,4781)>>>
|
4501 ttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagca 4560
AATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGT
4561 tgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaa 4620
ACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTT
4621 gtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgccca 4680
CATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGT
SV40 origin(4680,4757)>>>
|
4681 tcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttt 4740
AGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAA
SfiI
|
ORF_2 rf(1)(4747,5415)>>>
| |
4741 ttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggag 4800
AATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTC
puro marker(4816,5415)>>>
|
4801 gcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgac 4860
CGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTG
4861 gtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccac 4920
CAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTG
4921 accgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacg 4980
TGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGC
4981 cgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtc 5040
GCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAG
5041 tggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatg 5100
ACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTAC
5101 gccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgccg 5160
CGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGC
5161 caccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccag 5220
GTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTC
5221 ggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggg 5280
CCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCC
5281 gtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggc 5340
CACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCG
5341 ttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgc 5400
AAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCG
5401 aagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaaga 5460
TTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCT
5461 ttgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatg 5520
AACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTAC
5521 cctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcc 5580
GGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGG
5581 tggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgc 5640
ACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACG
5641 actgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctt 5700
TGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAA
5701 tccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcctt 5760
AGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAA
5761 gcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgggg 5820
CGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCC
5821 aagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacg 5880
TTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGC
5881 tccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctg 5940
AGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGAC
5941 ccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcccttt 6000
GGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAA
delta_U3 other(6025,6077)>>>
|
6001 gggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatc 6060
CCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAG
HIV-1_5_LTR other(6078,6258)>>>
|
6061 tgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctg 6120
ACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGAC
6121 gctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtag 6180
CGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATC
6181 tgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcag 6240
ACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTC
6241 tgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttg 6300
ACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAAC
6301 caaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttaca 6360
GTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGT
6361 aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagtt 6420
TTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAA
6421 gtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcgga 6480
CACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCT
6481 gaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaac 6540
CTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTG
6541 gatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctctt 6600
CTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAA
6601 ccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag 6660
GGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTC
6661 ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaaca 6720
GAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGT
pBR322 origin(6764,7383)<<<
|
6721 tgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttt 6780
ACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAA
6781 tccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggc 6840
AGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCG
6841 gaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct 6900
CTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGA
6901 ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcg 6960
GAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGC
6961 tggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcca 7020
ACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGT
7021 agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaact 7080
TCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGA
7081 atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggta 7140
TAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCAT
7141 acaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggccta 7200
TGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGAT
7201 actacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacct 7260
TGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGA
7261 tcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt 7320
AGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAA
7321 tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttga 7380
AAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACT
7381 tcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtca 7440
AGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGT
7441 tgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaat 7500
ACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTA
7501 caatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgagg 7560
GTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCC
7561 cacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgt 7620
GTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACA
7621 agataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgag 7680
TCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTC
7681 acccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagc 7740
TGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCG
7741 gcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaag 7800
CGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTC
7801 ctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcac 7860
GATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTG
7861 ctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgt 7920
GATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACA
7921 cagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttg 7980
GTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAAC
NEOKAN prom(8036,8085)>>>
|
7981 cagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccgga 8040
GTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCT
8041 attgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggc 8100
TAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCG
BclI
|
8101 tttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatg 8160
AAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTAC
NTP_II marker(8177,8965)>>>
|
ORF_4 rf(2)(8174,8968)>>>
| |
8161 aggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggt 8220
TCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCA
8221 ggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgt 8280
CCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCA
8281 gttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgc 8340
CAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACG
8341 cctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttcc 8400
GGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGG
8401 ttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcga 8460
AACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCT
8461 agtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcat 8520
TCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTA
8521 ggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccacca 8580
CCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGT
8581 agcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagga 8640
TCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCT
8641 tgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggc 8700
ACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCG
8701 gagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatat 8760
CTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATA
8761 catggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcgga 8820
GTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCT
8821 ccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatg 8880
GGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTAC
8881 ggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcctt 8940
CCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAA
8941 ctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcat 9000
GATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTA
9001 tttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcga 9060
AAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCT
f1 origin(9073,9378)<<<
|
9061 tagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactcca 9120
ATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGT
9121 acgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccca 9180
TGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGT
9181 aatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcc 9240
TTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGG
9241 cccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaag 9300
GGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTC
9301 cgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccacca 9360
GCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGT
9361 cacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaatta 9420
GTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAAT
9421 attcttaattaacatcatcaataatatacctt 9452
TAAGAATTAATTGTAGTAGTTATTATATGGAA