pLenti-III-EF1alpha

Vector Name pLenti-III-EF1alpha
VectorType Lentivirus
Antibiotic Information Puromycin
Sequencing Primers EF1a sequencing primer
5'--TCA GAC AGT GGT TCA AAG--3'
SV40 reverse sequencing primer
5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc 1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg 2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa 2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg 2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa 2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc 2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg 2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg 2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc 2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg 2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg 2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac 2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg 2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg 2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt 2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca 3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag 3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata 3121 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag 3181 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga 3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 3481 aagtagtgag gaggcttttt tggaggccat gaccgagtac aagcccacgg tgcgcctcgc 3541 cacccgcgac gacgtccctc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3601 cgccacgcgc cacaccgtgg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 3661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3841 gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 3901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 3961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4081 gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc tggattacaa 4141 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 4201 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 4261 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 4321 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 4381 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 4441 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 4501 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 4561 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 4621 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc tcagacgagt 4681 cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat tcactcccaa 4741 cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggcatcta 5161 tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa 5221 tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 5281 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6121 agggattttg gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa 6181 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac 6241 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 6301 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc 6361 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg 6421 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga 6481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 6541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 6601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 6661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 6721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 6961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 7021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 7081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 7141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 7201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 7261 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 7321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 7381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 7441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 7501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 7561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 7621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 7681 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 7741 ataatatacc ttttttggat tgaagccaat atgataatga gggggtggag tttgtgacgt 7801 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca 7861 agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc 7921 ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta gtaaatttgg 7981 gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg aagtgaaatc 8041 tgaataattt tgtgttactc atagcgcgta atactctcat gagcggatac atatttgaat 8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8161 acgtaactat aacggtccta aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc 8221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc 8281 atgccgattg gtggaagtaa ggtggtacga t //
                            LOCUS dna 8311 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" ORF 1775..2755 /sequence="ORF_4 rf(4)" Promoter 1838..3015 /gene="EF1a prom" misc_binding 3087..3092 /dbxref="REBASE:EcoRI" misc_binding 3111..3116 /dbxref="REBASE:HincII" misc_binding 3117..3122 /dbxref="REBASE:EcoRV" misc_binding 3129..3136 /dbxref="REBASE:NotI" misc_binding 3138..3143 /dbxref="REBASE:BamHI" misc_binding 3144..3149 /dbxref="REBASE:XbaI" Promoter 3206..3474 /gene="SV40 prom" Rep_Origin 3373..3450 /gene="SV40 origin" ORF 3440..4108 /sequence="ORF_2 rf(2)" misc_binding 3453..3465 /dbxref="REBASE:SfiI" Marker 3509..4108 /gene="puro marker" Other Gene 4718..4770 /gene="delta_U3 other" Other Gene 4771..4951 /gene="HIV-1_5_LTR other" Rep_Origin 5458..6077 /gene="pBR322 origin" Promoter 6335..6384 /gene="NEOKAN prom" misc_binding 6442..6447 /dbxref="REBASE:BclI" ORF 6473..7267 /sequence="ORF_3 rf(2)" Marker 6476..7264 /gene="NTP_II marker" Rep_Origin 7372..7678 /gene="f1 origin" Other Gene 7733..7834 /gene="RITR other" Other Gene 7733..7834 /gene="LITR other" Other Gene 7914..8062 /gene="Encap other" BASE COUNT 1974 a 2052 c 2371 g 1914 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc 1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg 2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa 2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg 2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa 2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc 2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg 2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg 2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc 2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg 2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg 2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac 2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg 2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg 2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt 2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca 3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag 3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata 3121 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag 3181 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga 3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 3481 aagtagtgag gaggcttttt tggaggccat gaccgagtac aagcccacgg tgcgcctcgc 3541 cacccgcgac gacgtccctc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3601 cgccacgcgc cacaccgtgg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 3661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3841 gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 3901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 3961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4081 gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc tggattacaa 4141 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 4201 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 4261 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 4321 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 4381 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 4441 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 4501 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 4561 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 4621 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc tcagacgagt 4681 cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat tcactcccaa 4741 cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggcatcta 5161 tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa 5221 tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 5281 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6121 agggattttg gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa 6181 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac 6241 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 6301 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc 6361 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg 6421 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga 6481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 6541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 6601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 6661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 6721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 6961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 7021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 7081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 7141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 7201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 7261 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 7321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 7381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 7441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 7501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 7561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 7621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 7681 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 7741 ataatatacc ttttttggat tgaagccaat atgataatga gggggtggag tttgtgacgt 7801 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca 7861 agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc 7921 ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta gtaaatttgg 7981 gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg aagtgaaatc 8041 tgaataattt tgtgttactc atagcgcgta atactctcat gagcggatac atatttgaat 8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8161 acgtaactat aacggtccta aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc 8221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc 8281 atgccgattg gtggaagtaa ggtggtacga t //
                        
***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60 GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC HIV-1_5_LTR other(109,289)>>> | 61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120 GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC 121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180 AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA 181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240 GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA 241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300 TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT 301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360 TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC HIV-1_psi_pack other(400,444)>>> | 361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420 GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT 421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480 GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT NruI | 481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540 AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT 541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600 TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC 601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660 TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA 661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720 GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT 721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780 ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT 781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840 TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA 841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900 ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG RRE reg(951,1184)>>> | 901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960 TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC ORF_1 rf(1)(994,1695)>>> | 961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020 AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC 1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080 CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA 1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140 TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT 1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200 TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG 1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260 AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA 1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320 GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG 1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380 TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT 1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440 CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC 1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500 GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA 1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560 AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC 1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620 TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT 1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680 CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA 1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740 CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA ORF_4 rf(4)(1775,2755)<<< | 1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800 TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT EF1a prom(1838,3015)>>> | 1801 aaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtcagtgggc 1860 TTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCG 1861 agagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccgg 1920 TCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCC 1921 tgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcct 1980 ACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGA 1981 ttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttt 2040 AAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAA 2041 tcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctgg 2100 AGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACC 2101 cctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtg 2160 GGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCAC 2161 attcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa 2220 TAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATT 2221 ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtg 2280 CCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCAC 2281 cgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaa 2340 GCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATT 2341 aatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggc 2400 TTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCG 2401 caagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcg 2460 GTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGC 2461 tcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgg 2520 AGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCC 2521 gggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgcc 2580 CCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGG 2581 ccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg 2640 GGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGC 2641 cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcg 2700 GAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGC 2701 ggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgac 2760 CCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTG 2761 tccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg 2820 AGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGC 2821 tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtg 2880 AGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCAC 2881 gagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcccttttt 2940 CTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAA 2941 gagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttcca 3000 CTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGT 3001 tttcaggtgtcgtgaggaatttcgactgctagctggtacctgaattgagtacttacgtag 3060 AAAGTCCACAGCACTCCTTAAAGCTGACGATCGACCATGGACTTAACTCATGAATGCATC EcoRI HincII | | 3061 gtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctgtcgacgata 3120 CATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTAT EcoRV NotI BamHI XbaI | | | | 3121 tcgggcccgcggccgctggatcctctagactgcagctcgagtacccatacgacgtcccag 3180 AGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTC SV40 prom(3206,3474)>>> | 3181 actacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcaga 3240 TGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCT 3241 agtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcc 3300 TCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGG 3301 ccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgccc 3360 GGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGG SV40 origin(3373,3450)>>> | 3361 ctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc 3420 GATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCG SfiI | ORF_2 rf(2)(3440,4108)>>> | | 3421 tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccag 3480 ACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTC puro marker(3509,4108)>>> | 3481 aagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgc 3540 TTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCG 3541 cacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccc 3600 GTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGG 3601 cgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaaga 3660 GCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCT 3661 actcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgc 3720 TGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCG 3721 cgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagat 3780 GCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTA 3781 cggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagg 3840 GCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCC 3841 gctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctc 3900 CGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAG 3901 gcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggc 3960 CGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCG 3961 cgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttcta 4020 GCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGAT 4021 cgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctg 4080 GCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGAC 4081 gtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaa 4140 CACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTT 4141 aatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggata 4200 TTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTAT 4201 cgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctc 4260 GCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAG 4261 cttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacg 4320 GAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGC 4321 tggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccac 4380 ACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTG 4381 ctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcat 4440 GACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTA 4441 cgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgt 4500 GCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCA 4501 ggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggat 4560 CCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTA 4561 tctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttc 4620 AGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAG 4621 ccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagt 4680 GGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCA delta_U3 other(4718,4770)>>> | 4681 cggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaa 4740 GCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTT HIV-1_5_LTR other(4771,4951)>>> | 4741 cgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcc 4800 GCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGG 4801 tgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttga 4860 ACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACT 4861 gtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcaga 4920 CACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCT 4921 cccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcag 4980 GGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTC 4981 tatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagct 5040 ATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGA 5041 tataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttca 5100 ATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGT 5101 ctgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatcta 5160 GACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGAT 5161 tgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaa 5220 ACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATT 5221 tgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaataccg 5280 ACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGC 5281 catcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg 5340 GTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGC 5341 gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataa 5400 CGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATT pBR322 origin(5458,6077)<<< | 5401 cgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgc 5460 GCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCG 5461 gttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctc 5520 CAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAG 5521 aagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaag 5580 TTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTC 5581 ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttct 5640 GAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGA 5641 cccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgta 5700 GGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACAT 5701 ggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgc 5760 CCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCG 5761 cttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggc 5820 GAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCG 5821 agcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttctt 5880 TCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAA 5881 gaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct 5940 CTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGA 5941 gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgc 6000 CTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCG 6001 tggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctca 6060 ACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGT 6061 agaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtta 6120 TCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAAT 6121 agggattttggccatgagattatcaaaaaggatcttcacctagatccttttcacgtagaa 6180 TCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTT 6181 agccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggac 6240 TCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTG 6241 aagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgata 6300 TTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTAT NEOKAN prom(6335,6384)>>> | 6301 gctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctc 6360 CGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAG 6361 tggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctg 6420 ACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGAC NTP_II marker(6476,7264)>>> | BclI ORF_3 rf(2)(6473,7267)>>> | | | 6421 atggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattga 6480 TACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACT 6481 acaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatga 6540 TGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACT 6541 ctgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 6600 GACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCC 6601 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacga 6660 CGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCT 6661 ggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgt 6720 CCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCA 6721 tgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcct 6780 ACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGA 6781 gtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggct 6840 CAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGA 6841 gcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcg 6900 CGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGC 6901 agcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatca 6960 TCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGT 6961 ggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagga 7020 CCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCT 7021 tctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctt 7080 AGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAA 7081 ttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgtt 7140 AAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAA 7141 ggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgct 7200 CCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGA 7201 ttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagtt 7260 AATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAA 7261 cttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaa 7320 GAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTT f1 origin(7372,7678)<<< | 7321 tcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccag 7380 AGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTC 7381 tttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccg 7440 AAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGC 7441 tctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcga 7500 AGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCT 7501 ggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggg 7560 CCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCC 7561 gaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctaggg 7620 CTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCC 7621 cgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgc 7680 GCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCG RITR other(7733,7834)<<< | LITR other(7733,7834)>>> | 7681 cgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatca 7740 GCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGT 7741 ataatataccttttttggattgaagccaatatgataatgagggggtggagtttgtgacgt 7800 TATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCA 7801 ggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgca 7860 CCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGT Encap other(7914,8062)>>> | 7861 agtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgcc 7920 TCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGG 7921 ggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgg 7980 CCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACC 7981 gcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatc 8040 CGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAG 8041 tgaataattttgtgttactcatagcgcgtaatactctcatgagcggatacatatttgaat 8100 ACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTA 8101 gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg 8160 CATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGAC 8161 acgtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtc 8220 TGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAG 8221 ttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgc 8280 AACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACG 8281 atgccgattggtggaagtaaggtggtacgat 8311 TACGGCTAACCACCTTCATTCCACCATGCTA
                            LOCUS dna 8311 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" ORF 1775..2755 /sequence="ORF_4 rf(4)" Promoter 1838..3015 /gene="EF1a prom" misc_binding 3087..3092 /dbxref="REBASE:EcoRI" misc_binding 3111..3116 /dbxref="REBASE:HincII" misc_binding 3117..3122 /dbxref="REBASE:EcoRV" misc_binding 3129..3136 /dbxref="REBASE:NotI" misc_binding 3138..3143 /dbxref="REBASE:BamHI" misc_binding 3144..3149 /dbxref="REBASE:XbaI" Promoter 3206..3474 /gene="SV40 prom" Rep_Origin 3373..3450 /gene="SV40 origin" ORF 3440..4108 /sequence="ORF_2 rf(2)" misc_binding 3453..3465 /dbxref="REBASE:SfiI" Marker 3509..4108 /gene="puro marker" Other Gene 4718..4770 /gene="delta_U3 other" Other Gene 4771..4951 /gene="HIV-1_5_LTR other" Rep_Origin 5458..6077 /gene="pBR322 origin" Promoter 6335..6384 /gene="NEOKAN prom" misc_binding 6442..6447 /dbxref="REBASE:BclI" ORF 6473..7267 /sequence="ORF_3 rf(2)" Marker 6476..7264 /gene="NTP_II marker" Rep_Origin 7372..7678 /gene="f1 origin" Other Gene 7733..7834 /gene="RITR other" Other Gene 7733..7834 /gene="LITR other" Other Gene 7914..8062 /gene="Encap other" BASE COUNT 1974 a 2052 c 2371 g 1914 t 0 others