pLenti-III-CMV-GFP
| Vector Name | pLenti-III-CMV-GFP |
| VectorType | Lentiviral vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | There is a C-terminal linker and polyarginine tag on the GFP (ESGGGGSPGRRRRRRRRR) which allows for purification. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagccgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
3121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
3181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
3241 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
3301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
3361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
3421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
3481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
3541 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
3601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
3661 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
3721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
3781 agctgtacaa ggaaagcggc ggcggcggca gcccaggccg cagacgccgc agacgccgca
3841 gacgctgagg taccccagtg tggtggcctg caggtgaatt cactagtacc ggtaggcctg
3901 tcgacgatat cgggcccgcg gccgctggat cctctagact gcagctcgag tacccatacg
3961 acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg gaaagtcccc aggctcccca
4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
4141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4261 ctattccaga agtagtgagg aggctttttt ggaggccatg accgagtaca agcccacggt
4321 gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc
4381 cgactacccc gccacgcgcc acaccgtgga cccggaccgc cacatcgagc gggtcaccga
4441 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
4501 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
4561 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
4621 gatggaaggg ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
4681 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
4741 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
4801 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
4861 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttccggaa atcaacctct
4921 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
4981 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
5041 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
5101 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
5161 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
5221 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
5281 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
5341 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
5401 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct
5461 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt
5521 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
5581 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
5641 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
5701 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
5761 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
5821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
5881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
5941 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt
6001 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga
6061 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
6721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
6961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
7021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
7201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7321 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
7381 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
7441 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
7501 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
7561 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa
7621 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
7681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
7741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
7801 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
7861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
7921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
7981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
8041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
8101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
8161 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
8221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
8281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
8341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
8401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
8461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat
8521 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca
8581 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
8641 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta
8701 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
8761 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
8821 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca
8881 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
LOCUS dna 8934 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3848
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3063..4043
/sequence="ORF_5 rf(5)"
Reporter 3075..3791
/gene="EGFP reporter"
misc_binding 3849..3854
/dbxref="REBASE:KpnI"
misc_binding 3876..3881
/dbxref="REBASE:EcoRI"
misc_binding 3894..3899
/dbxref="REBASE:StuI"
misc_binding 3906..3911
/dbxref="REBASE:EcoRV"
misc_binding 3912..3917
/dbxref="REBASE:ApaI"
misc_binding 3918..3925
/dbxref="REBASE:NotI"
misc_binding 3927..3932
/dbxref="REBASE:BamHI"
misc_binding 3933..3938
/dbxref="REBASE:XbaI"
misc_binding 3945..3950
/dbxref="REBASE:XhoI"
Promoter 3995..4263
/gene="SV40 prom"
Rep_Origin 4162..4239
/gene="SV40 origin"
ORF 4229..4897
/sequence="ORF_2 rf(2)"
misc_binding 4242..4254
/dbxref="REBASE:SfiI"
Marker 4298..4897
/gene="puro marker"
Other Gene 5507..5559
/gene="delta_U3 other"
Other Gene 5560..5740
/gene="HIV-1_5_LTR other"
Rep_Origin 6246..6865
/gene="pBR322 origin"
Promoter 7518..7567
/gene="NEOKAN prom"
misc_binding 7625..7630
/dbxref="REBASE:BclI"
ORF 7656..8450
/sequence="ORF_4 rf(3)"
Marker 7659..8447
/gene="NTP_II marker"
Rep_Origin 8555..8860
/gene="f1 origin"
BASE COUNT 2233 a 2273 c 2433 g 1995 t 0 others
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_3 rf(3)(2901,3848)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
EGFP reporter(3075,3791)>>>
|
ORF_5 rf(5)(3063,4043)<<<
| |
3061 agctagccgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcc 3120
TCGATCGGCGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGG
3121 tggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagg 3180
ACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCC
3181 gcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccg 3240
CGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGC
3241 tgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctacc 3300
ACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGG
3301 ccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccagg 3360
GGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCC
3361 agcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcg 3420
TCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGC
3421 agggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca 3480
TCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGT
3481 acatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccg 3540
TGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGC
3541 acaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggca 3600
TGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGT
3601 gcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgc 3660
CGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACG
3661 tgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagc 3720
ACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCG
3721 gcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacg 3780
CGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGC
3781 agctgtacaaggaaagcggcggcggcggcagcccaggccgcagacgccgcagacgccgca 3840
TCGACATGTTCCTTTCGCCGCCGCCGCCGTCGGGTCCGGCGTCTGCGGCGTCTGCGGCGT
KpnI EcoRI StuI
| | |
3841 gacgctgaggtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctg 3900
CTGCGACTCCATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGAC
NotI
|
EcoRV ApaI BamHI XbaI XhoI
| | | | | |
3901 tcgacgatatcgggcccgcggccgctggatcctctagactgcagctcgagtacccatacg 3960
AGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGC
SV40 prom(3995,4263)>>>
|
3961 acgtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctcccca 4020
TGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGT
4021 gcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtcc 4080
CGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGG
4081 ccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccata 4140
GGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTAT
SV40 origin(4162,4239)>>>
|
4141 gtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccg 4200
CAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGC
SfiI
|
ORF_2 rf(2)(4229,4897)>>>
| |
4201 ccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgag 4260
GGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTC
puro marker(4298,4897)>>>
|
4261 ctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggt 4320
GATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCA
4321 gcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgc 4380
CGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCG
4381 cgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccga 4440
GCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCT
4441 gctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcgga 4500
CGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCT
4501 cgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgtt 4560
GCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAA
4561 cgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaaca 4620
GCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGT
4621 gatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgt 4680
CTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCA
4681 cggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagt 4740
GCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCA
4741 ggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacct 4800
CCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGA
4801 ccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggacc 4860
GGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGG
4861 gcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctct 4920
CGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGA
4921 ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgct 4980
CCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGA
4981 atgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcat 5040
TACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTA
5041 tttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgt 5100
AAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACA
5101 caggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcat 5160
GTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTA
5161 tgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggc 5220
ACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCG
5221 ggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactga 5280
CCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACT
5281 caattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgc 5340
GTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACG
5341 cacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcgga 5400
GTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCT
5401 ccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccct 5460
GGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGA
delta_U3 other(5507,5559)>>>
|
5461 cagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaatt 5520
GTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAA
HIV-1_5_LTR other(5560,5740)>>>
|
5521 cactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccag 5580
GTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTC
5581 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 5640
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG
5641 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 5700
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT
5701 tccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatctt 5760
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAA
5761 attattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgttt 5820
TAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAA
5821 attgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagca 5880
TAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGT
5881 tttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtc 5940
AAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAG
5941 tggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggt 6000
ACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCA
6001 ctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggaga 6060
GACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCT
6061 aaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt 6120
TTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAA
6121 cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatca 6180
GCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGT
6181 ggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaa 6240
CCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTT
pBR322 origin(6246,6865)<<<
|
6241 aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat 6300
TTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTA
6301 cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc 6360
GCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGG
6361 cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc 6420
GGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGG
6421 gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagt 6480
CGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCA
6481 tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac 6540
AGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTG
6541 cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg 6600
GCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGC
6601 ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca 6660
GGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGT
6661 gagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgc 6720
CTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACG
6721 gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa 6780
CGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTT
6781 accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaa 6840
TGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTT
6841 ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac 6900
CCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTG
6901 tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta 6960
AGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAT
6961 aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt 7020
TTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCA
7021 taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccata 7080
ATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTAT
7081 gttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc 7140
CAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGG
7141 agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaac 7200
TCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTG
7201 cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag 7260
GTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTC
7261 tctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac 7320
AGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTG
7321 gttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaac 7380
CAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTG
7381 ggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcg 7440
CCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGC
7441 caaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttt 7500
GTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAA
NEOKAN prom(7518,7567)>>>
|
7501 tatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc 7560
ATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCG
7561 cctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaa 7620
GGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTT
NTP_II marker(7659,8447)>>>
|
BclI ORF_4 rf(3)(7656,8450)>>>
| | |
7621 gctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacg 7680
CGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGC
7681 caggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaa 7740
GTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTT
7741 tcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttg 7800
AGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAAC
7801 tcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgt 7860
AGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCA
7861 ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaa 7920
CCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTT
7921 gggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctc 7980
CCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAG
7981 ctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccgg 8040
GACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCC
8041 ctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgg 8100
GATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACC
8101 aagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg 8160
TTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGC
8161 aactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatg 8220
TTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTAC
8221 gcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgact 8280
CGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGA
8281 gtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattg 8340
CACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAAC
8341 ctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctc 8400
GACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAG
8401 ccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaa 8460
GGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATT
8461 aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttat 8520
TTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATA
f1 origin(8555,8860)<<<
|
8521 aaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcca 8580
TTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGT
8581 ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc 8640
GATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCG
8641 ccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctcta 8700
GGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGAT
8701 aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg 8760
TTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCAC
8761 gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg 8820
CGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGC
8821 gtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcca 8880
CAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGT
8881 ttcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 8934
AAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA