pLenti-III-CFP-N
| Vector Name | pLenti-III-CFP-N |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 gtatataacg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
2521 ctggtcgagc tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag
2581 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
2641 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
2701 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
2761 gagcgtacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
2821 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
2881 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
2941 gacaagcaga agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc
3001 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
3061 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
3121 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
3181 gagctgtaca agcccggggc tagccaattg agtacttacg taggtacccc agtgtggtgg
3241 cctgcaggtg aattcactag taccggtagg cctgtcgacg atatcgggcc cgcggccgct
3301 ggatcctcta gactgcagct cgagtaccca tacgacgtcc cagactacgc ttgagtttaa
3361 acacgcgtgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
3421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
3541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
3601 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
3661 ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
3721 ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
3781 tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
3841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
3901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
3961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc
4021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
4081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
4141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
4201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
4261 ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga
4321 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4381 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4441 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
4501 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
4561 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
4621 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
4681 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
4741 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
4801 ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc
4861 cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct
4921 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
4981 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
5041 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
5101 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
5161 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
5221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5281 tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa
5341 gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacggat
5401 gtcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
5461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
5521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
5581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
5641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
5701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
5761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
5821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
6001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
6061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
6121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
6181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
6241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggccatga
6301 gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
6361 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
6421 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
6481 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
6541 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
6601 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
6661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
6721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
6781 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
6841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
6901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
6961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
7021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
7081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
7141 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
7201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
7261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
7321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
7381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
7441 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
7501 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
7561 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
7621 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
7681 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
7741 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
7801 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
7861 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat accttttttg
7921 gattgaagcc aatatgataa tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa
7981 cggggcgggt gacgtagtag tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat
8041 gtaagcgacg gatgtggcaa aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg
8101 acaattttcg cgcggtttta ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat
8161 ttggccattt tcgcgggaaa actgaataag aggaagtgaa atctgaataa ttttgtgtta
8221 ctcatagcgc gtaatactct catgagcgga tacatatttg aatgtattta gaaaaataaa
8281 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtaac tataacggtc
8341 ctaaggtagc gaaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
8401 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
8461 taaggtggta cgat
LOCUS dna 8474 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
ORF 2443..3417
/sequence="ORF_5 rf(6)"
Reporter 2476..3192
/gene="EGFP reporter"
ORF 2476..3354
/sequence="ORF_2 rf(1)"
misc_binding 3193..3198
/dbxref="REBASE:SmaI"
misc_binding 3193..3198
/dbxref="REBASE:XmaI"
misc_binding 3223..3228
/dbxref="REBASE:KpnI"
misc_binding 3250..3255
/dbxref="REBASE:EcoRI"
misc_binding 3268..3273
/dbxref="REBASE:StuI"
misc_binding 3274..3279
/dbxref="REBASE:HincII"
misc_binding 3280..3285
/dbxref="REBASE:EcoRV"
misc_binding 3286..3291
/dbxref="REBASE:ApaI"
misc_binding 3292..3299
/dbxref="REBASE:NotI"
misc_binding 3301..3306
/dbxref="REBASE:BamHI"
misc_binding 3307..3312
/dbxref="REBASE:XbaI"
misc_binding 3319..3324
/dbxref="REBASE:XhoI"
Promoter 3369..3637
/gene="SV40 prom"
Rep_Origin 3536..3613
/gene="SV40 origin"
ORF 3603..4271
/sequence="ORF_3 rf(3)"
misc_binding 3616..3628
/dbxref="REBASE:SfiI"
Marker 3672..4271
/gene="puro marker"
Other Gene 4881..4933
/gene="delta_U3 other"
Other Gene 4934..5114
/gene="HIV-1_5_LTR other"
Rep_Origin 5621..6240
/gene="pBR322 origin"
Promoter 6498..6547
/gene="NEOKAN prom"
misc_binding 6605..6610
/dbxref="REBASE:BclI"
ORF 6636..7430
/sequence="ORF_4 rf(3)"
Marker 6639..7427
/gene="NTP_II marker"
Rep_Origin 7535..7841
/gene="f1 origin"
Other Gene 7896..7997
/gene="RITR other"
Other Gene 7896..7997
/gene="LITR other"
Other Gene 8077..8225
/gene="Encap other"
BASE COUNT 2109 a 2154 c 2327 g 1884 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 gtatataacg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
2521 ctggtcgagc tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag
2581 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
2641 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
2701 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
2761 gagcgtacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
2821 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
2881 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
2941 gacaagcaga agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc
3001 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
3061 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
3121 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
3181 gagctgtaca agcccggggc tagccaattg agtacttacg taggtacccc agtgtggtgg
3241 cctgcaggtg aattcactag taccggtagg cctgtcgacg atatcgggcc cgcggccgct
3301 ggatcctcta gactgcagct cgagtaccca tacgacgtcc cagactacgc ttgagtttaa
3361 acacgcgtgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
3421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
3541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
3601 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
3661 ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
3721 ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
3781 tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
3841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
3901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
3961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc
4021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
4081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
4141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
4201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
4261 ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga
4321 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4381 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4441 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
4501 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
4561 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
4621 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
4681 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
4741 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
4801 ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc
4861 cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct
4921 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
4981 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
5041 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
5101 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
5161 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
5221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5281 tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa
5341 gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacggat
5401 gtcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
5461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
5521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
5581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
5641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
5701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
5761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
5821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
6001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
6061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
6121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
6181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
6241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggccatga
6301 gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
6361 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
6421 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
6481 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
6541 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
6601 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
6661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
6721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
6781 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
6841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
6901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
6961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
7021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
7081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
7141 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
7201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
7261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
7321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
7381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
7441 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
7501 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
7561 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
7621 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
7681 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
7741 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
7801 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
7861 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat accttttttg
7921 gattgaagcc aatatgataa tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa
7981 cggggcgggt gacgtagtag tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat
8041 gtaagcgacg gatgtggcaa aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg
8101 acaattttcg cgcggtttta ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat
8161 ttggccattt tcgcgggaaa actgaataag aggaagtgaa atctgaataa ttttgtgtta
8221 ctcatagcgc gtaatactct catgagcgga tacatatttg aatgtattta gaaaaataaa
8281 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtaac tataacggtc
8341 ctaaggtagc gaaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
8401 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
8461 taaggtggta cgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC
1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920
GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT
1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980
CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG
NdeI
|
1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040
GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG
2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100
CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC
2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160
GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT
2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220
TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT
CMV prom(2279,2360)>>>
|
2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280
TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC
2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340
GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG
2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400
CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT
tetO reg(2416,2455)>>> ORF_5 rf(6)(2443,3417)<<<
| |
2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460
CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC
EGFP reporter(2476,3192)>>>
|
ORF_2 rf(1)(2476,3354)>>>
|
2461 gtatataacgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatc 2520
CATATATTGCGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAG
2521 ctggtcgagctggacggcgacgtaaacggccacaggttcagcgtgtccggcgagggcgag 2580
GACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTCCAAGTCGCACAGGCCGCTCCCGCTC
2581 ggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgccc 2640
CCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGG
2641 gtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctac 2700
CACGGGACCGGGTGGGAGCACTGGTGGGACTGGACCCCGCACGTCACGAAGTCGGCGATG
2701 cccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccag 2760
GGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTC
2761 gagcgtaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttc 2820
CTCGCATGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAG
2821 gagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc 2880
CTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCG
2881 aacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgcc 2940
TTGTAGGACCCCGTGTTCGACCTCATGTTGATGTAGTCGGTGTTGCAGATATAGTGGCGG
2941 gacaagcagaagaacggcatcaaggcccacttcaagatccgccacaacatcgaggacggc 3000
CTGTTCGTCTTCTTGCCGTAGTTCCGGGTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCG
3001 agcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctg 3060
TCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGAC
3061 ctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaag 3120
GACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTC
3121 cgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggac 3180
GCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTG
SmaI
|
XmaI KpnI
| | |
3181 gagctgtacaagcccggggctagccaattgagtacttacgtaggtaccccagtgtggtgg 3240
CTCGACATGTTCGGGCCCCGATCGGTTAACTCATGAATGCATCCATGGGGTCACACCACC
EcoRV NotI
| |
EcoRI StuI HincII ApaI
| | | | | |
3241 cctgcaggtgaattcactagtaccggtaggcctgtcgacgatatcgggcccgcggccgct 3300
GGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGA
BamHI XbaI XhoI
| | |
3301 ggatcctctagactgcagctcgagtacccatacgacgtcccagactacgcttgagtttaa 3360
CCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATT
SV40 prom(3369,3637)>>>
|
3361 acacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgca 3420
TGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGT
3421 tctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtat 3480
AGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATA
SV40 origin(3536,3613)>>>
|
3481 gcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatccc 3540
CGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGG
3541 gcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttat 3600
CGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATA
SfiI
|
ORF_3 rf(3)(3603,4271)>>>
| |
3601 ttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggctt 3660
AATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAA
puro marker(3672,4271)>>>
|
3661 ttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcc 3720
AAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGG
3721 ctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccg 3780
GAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGC
3781 tggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcg 3840
ACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGC
3841 tcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctgga 3900
AGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCT
3901 ccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccg 3960
GGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGC
3961 agttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgccgcacc 4020
TCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGG
4021 ggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggca 4080
CCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGT
4081 agggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgc 4140
TCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACG
4141 ccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttca 4200
GGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGT
4201 ccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagc 4260
GGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCG
4261 ccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattga 4320
GGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACT
4321 ctggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctt 4380
GACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAA
4381 tgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggt 4440
ACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCA
4441 tgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactg 4500
ACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGAC
4501 tgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccg 4560
ACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGC
4561 ggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgccc 4620
CCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGG
4621 gctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagc 4680
CGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCG
4681 tgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtcct 4740
ACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGA
4741 tctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccgg 4800
AGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCC
4801 ctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggc 4860
GAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCG
delta_U3 other(4881,4933)>>>
|
4861 cgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgct 4920
GCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGA
HIV-1_5_LTR other(4934,5114)>>>
|
4921 ttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggcta 4980
AAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGAT
4981 actagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtg 5040
TGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACAC
5041 tgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtg 5100
ACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACAC
5101 gaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaa 5160
CTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTT
5161 gaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaata 5220
CTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTAT
5221 aagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtgg 5280
TTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACC
5281 tttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaa 5340
AAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTT
5341 gaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacggat 5400
CTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTA
5401 gtcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccg 5460
CAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGC
5461 cttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc 5520
GAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAG
5521 actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgt 5580
TGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACA
pBR322 origin(5621,6240)<<<
|
5581 gagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcc 5640
CTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGG
5641 ataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaa 5700
TATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTT
5701 acccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctc 5760
TGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAG
5761 ctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtgg 5820
GACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACC
5821 cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagc 5880
GCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCG
5881 tgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatc 5940
ACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAG
5941 gtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaaca 6000
CAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGT
6001 ggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaact 6060
CCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGA
6061 acggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcg 6120
TGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGC
6121 gaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttt 6180
CTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAA
6181 ttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatct 6240
AACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGA
6241 tttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggccatga 6300
AAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACT
6301 gattatcaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaac 6360
CTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTG
6361 ggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcg 6420
CCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGC
6421 caaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttt 6480
GTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAA
NEOKAN prom(6498,6547)>>>
|
6481 tatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc 6540
ATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCG
6541 cctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaa 6600
GGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTT
NTP_II marker(6639,7427)>>>
|
BclI ORF_4 rf(3)(6636,7430)>>>
| | |
6601 gctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacg 6660
CGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGC
6661 caggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaa 6720
GTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTT
6721 tcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttg 6780
AGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAAC
6781 tcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgt 6840
AGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCA
6841 ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaa 6900
CCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTT
6901 gggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctc 6960
CCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAG
6961 ctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccgg 7020
GACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCC
7021 ctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgg 7080
GATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACC
7081 aagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg 7140
TTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGC
7141 aactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatg 7200
TTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTAC
7201 gcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgact 7260
CGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGA
7261 gtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattg 7320
CACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAAC
7321 ctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctc 7380
GACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAG
7381 ccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaa 7440
GGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATT
7441 aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat 7500
TTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATA
f1 origin(7535,7841)<<<
|
7501 aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtcca 7560
TTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGT
7561 ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc 7620
GATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCG
7621 ccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcacta 7680
GGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGAT
7681 aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg 7740
TTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCAC
7741 gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg 7800
CGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGC
7801 gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcc 7860
CAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGG
LITR other(7896,7997)>>>
|
RITR other(7896,7997)<<<
|
7861 attcgccattcaggatcgaattaattcttaattaacatcatcaataatataccttttttg 7920
TAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAAC
7921 gattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaa 7980
CTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTT
7981 cggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacat 8040
GCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTA
Encap other(8077,8225)>>>
|
8041 gtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtg 8100
CATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCAC
8101 acaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagat 8160
TGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTA
8161 ttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgtta 8220
AACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAAT
8221 ctcatagcgcgtaatactctcatgagcggatacatatttgaatgtatttagaaaaataaa 8280
GAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTT
8281 caaataggggttccgcgcacatttccccgaaaagtgccacctgacgtaactataacggtc 8340
GTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAG
8341 ctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacga 8400
GATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCT
8401 tgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaag 8460
ACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTC
8461 taaggtggtacgat 8474
ATTCCACCATGCTA
LOCUS dna 8474 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
ORF 2443..3417
/sequence="ORF_5 rf(6)"
Reporter 2476..3192
/gene="EGFP reporter"
ORF 2476..3354
/sequence="ORF_2 rf(1)"
misc_binding 3193..3198
/dbxref="REBASE:SmaI"
misc_binding 3193..3198
/dbxref="REBASE:XmaI"
misc_binding 3223..3228
/dbxref="REBASE:KpnI"
misc_binding 3250..3255
/dbxref="REBASE:EcoRI"
misc_binding 3268..3273
/dbxref="REBASE:StuI"
misc_binding 3274..3279
/dbxref="REBASE:HincII"
misc_binding 3280..3285
/dbxref="REBASE:EcoRV"
misc_binding 3286..3291
/dbxref="REBASE:ApaI"
misc_binding 3292..3299
/dbxref="REBASE:NotI"
misc_binding 3301..3306
/dbxref="REBASE:BamHI"
misc_binding 3307..3312
/dbxref="REBASE:XbaI"
misc_binding 3319..3324
/dbxref="REBASE:XhoI"
Promoter 3369..3637
/gene="SV40 prom"
Rep_Origin 3536..3613
/gene="SV40 origin"
ORF 3603..4271
/sequence="ORF_3 rf(3)"
misc_binding 3616..3628
/dbxref="REBASE:SfiI"
Marker 3672..4271
/gene="puro marker"
Other Gene 4881..4933
/gene="delta_U3 other"
Other Gene 4934..5114
/gene="HIV-1_5_LTR other"
Rep_Origin 5621..6240
/gene="pBR322 origin"
Promoter 6498..6547
/gene="NEOKAN prom"
misc_binding 6605..6610
/dbxref="REBASE:BclI"
ORF 6636..7430
/sequence="ORF_4 rf(3)"
Marker 6639..7427
/gene="NTP_II marker"
Rep_Origin 7535..7841
/gene="f1 origin"
Other Gene 7896..7997
/gene="RITR other"
Other Gene 7896..7997
/gene="LITR other"
Other Gene 8077..8225
/gene="Encap other"
BASE COUNT 2109 a 2154 c 2327 g 1884 t 0 others