pLenti-III-CFP-C
Vector Name | pLenti-III-CFP-C |
VectorType | Lentiviral Vector |
Antibiotic Information | Puromycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actcgagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2641 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc 2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2761 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca 2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac 2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3001 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca 3061 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3301 caagtaatcg agtacccata cgacgtccca gactacgctt gagtttaaac acgcgtggtg 3361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3421 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3481 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3541 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3601 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcca 3661 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac 3721 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtg gacccggacc 3781 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 3841 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 3901 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 3961 cccggctggc cgcgcagcaa cagatggaag ggctcctggc gccgcaccgg cccaaggagc 4021 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 4081 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 4141 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 4201 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 4261 cgcgttccgg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 4321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 4381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 4441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 4501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 4561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 4621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 4681 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 4741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 4801 ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 4861 tgtccggatg gaagggctaa ttcactccca acgaatacaa gatctgcttt ttgcttgtac 4921 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 4981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 5041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 5101 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 5161 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 5221 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 5281 catcaatgta tcttatcatg tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa 5341 tggcattatg ggtattatgg gtctgcatta atgaatcggc caacggatgt cggtgtgaaa 5401 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 5461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6061 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 6181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggccatgaga ttatcaaaaa 6301 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc 6361 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc 6421 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa 6481 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa 6541 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag 6601 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 6661 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 6721 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 6781 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 6841 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 6901 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 6961 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 7021 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 7081 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 7141 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 7201 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 7261 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 7321 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 7381 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa 7441 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 7501 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 7561 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 7621 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 7681 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 7741 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 7801 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca 7861 ggatcgaatt aattcttaat taacatcatc aataatatac cttttttgga ttgaagccaa 7921 tatgataatg agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga 7981 cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga 8041 tgtggcaaaa gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg 8101 cggttttagg cggatgttgt agtaaatttg ggcgtaaccg agtaagattt ggccattttc 8161 gcgggaaaac tgaataagag gaagtgaaat ctgaataatt ttgtgttact catagcgcgt 8221 aatactctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8281 ccgcgcacat ttccccgaaa agtgccacct gacgtaacta taacggtcct aaggtagcga 8341 aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 8401 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 8461 at
LOCUS dna 8462 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" ORF 2354..3409 /sequence="ORF_5 rf(5)" Regulatory_Seq 2416..2455 /gene="tetO reg" misc_binding 2462..2467 /dbxref="REBASE:SmaI" misc_binding 2462..2467 /dbxref="REBASE:XmaI" misc_binding 2468..2473 /dbxref="REBASE:ClaI" misc_binding 2492..2497 /dbxref="REBASE:KpnI" misc_binding 2511..2516 /dbxref="REBASE:PstI" misc_binding 2519..2524 /dbxref="REBASE:EcoRI" misc_binding 2537..2542 /dbxref="REBASE:StuI" misc_binding 2543..2548 /dbxref="REBASE:HincII" misc_binding 2549..2554 /dbxref="REBASE:EcoRV" misc_binding 2555..2560 /dbxref="REBASE:ApaI" misc_binding 2561..2568 /dbxref="REBASE:NotI" misc_binding 2570..2575 /dbxref="REBASE:BamHI" misc_binding 2576..2581 /dbxref="REBASE:XbaI" misc_binding 2582..2587 /dbxref="REBASE:XhoI" Reporter 2588..3304 /gene="EGFP reporter" ORF 2588..3307 /sequence="ORF_2 rf(2)" Promoter 3357..3625 /gene="SV40 prom" Rep_Origin 3524..3601 /gene="SV40 origin" ORF 3591..4259 /sequence="ORF_3 rf(3)" misc_binding 3604..3616 /dbxref="REBASE:SfiI" Marker 3660..4259 /gene="puro marker" Other Gene 4869..4921 /gene="delta_U3 other" Other Gene 4922..5102 /gene="HIV-1_5_LTR other" Rep_Origin 5609..6228 /gene="pBR322 origin" Promoter 6486..6535 /gene="NEOKAN prom" misc_binding 6593..6598 /dbxref="REBASE:BclI" ORF 6624..7418 /sequence="ORF_4 rf(3)" Marker 6627..7415 /gene="NTP_II marker" Rep_Origin 7523..7829 /gene="f1 origin" Other Gene 7884..7985 /gene="RITR other" Other Gene 7884..7985 /gene="LITR other" Other Gene 8065..8213 /gene="Encap other" BASE COUNT 2107 a 2148 c 2324 g 1883 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga 2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag 2581 actcgagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2641 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc 2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2761 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca 2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac 2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3001 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca 3061 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3301 caagtaatcg agtacccata cgacgtccca gactacgctt gagtttaaac acgcgtggtg 3361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3421 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3481 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3541 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3601 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcca 3661 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac 3721 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtg gacccggacc 3781 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 3841 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 3901 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 3961 cccggctggc cgcgcagcaa cagatggaag ggctcctggc gccgcaccgg cccaaggagc 4021 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 4081 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 4141 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 4201 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 4261 cgcgttccgg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 4321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 4381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 4441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 4501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 4561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 4621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 4681 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 4741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 4801 ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 4861 tgtccggatg gaagggctaa ttcactccca acgaatacaa gatctgcttt ttgcttgtac 4921 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 4981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 5041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 5101 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 5161 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 5221 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 5281 catcaatgta tcttatcatg tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa 5341 tggcattatg ggtattatgg gtctgcatta atgaatcggc caacggatgt cggtgtgaaa 5401 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 5461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6061 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 6181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggccatgaga ttatcaaaaa 6301 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc 6361 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc 6421 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa 6481 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa 6541 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag 6601 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 6661 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 6721 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 6781 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 6841 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 6901 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 6961 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 7021 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 7081 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 7141 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 7201 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 7261 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 7321 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 7381 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa 7441 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 7501 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 7561 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 7621 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 7681 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 7741 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 7801 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca 7861 ggatcgaatt aattcttaat taacatcatc aataatatac cttttttgga ttgaagccaa 7921 tatgataatg agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga 7981 cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga 8041 tgtggcaaaa gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg 8101 cggttttagg cggatgttgt agtaaatttg ggcgtaaccg agtaagattt ggccattttc 8161 gcgggaaaac tgaataagag gaagtgaaat ctgaataatt ttgtgttact catagcgcgt 8221 aatactctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8281 ccgcgcacat ttccccgaaa agtgccacct gacgtaacta taacggtcct aaggtagcga 8341 aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 8401 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 8461 at //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60 GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC HIV-1_5_LTR other(109,289)>>> | 61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120 GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC 121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180 AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA 181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240 GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA 241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300 TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT 301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360 TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC HIV-1_psi_pack other(400,444)>>> | 361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420 GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT 421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480 GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT NruI | 481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540 AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT 541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600 TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC 601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660 TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA 661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720 GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT 721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780 ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT 781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840 TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA 841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900 ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG RRE reg(951,1184)>>> | 901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960 TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC ORF_1 rf(1)(994,1695)>>> | 961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020 AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC 1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080 CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA 1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140 TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT 1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200 TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG 1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260 AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA 1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320 GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG 1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380 TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT 1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440 CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC 1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500 GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA 1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560 AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC 1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620 TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT 1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680 CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA 1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740 CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA 1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800 TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT 1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860 TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC 1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920 GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT 1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980 CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG NdeI | 1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040 GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG 2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100 CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC 2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160 GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT 2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220 TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT CMV prom(2279,2360)>>> | 2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280 TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC 2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340 GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG ORF_5 rf(5)(2354,3409)<<< | 2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400 CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT tetO reg(2416,2455)>>> | 2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460 CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC SmaI | XmaI ClaI KpnI PstI | | | | | 2461 ccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgcaggtga 2520 GGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACGTCCACT EcoRV NotI | | EcoRI StuI HincII ApaI BamHI XbaI | | | | | | | | 2521 attcactagtaccggtaggcctgtcgacgatatcgggcccgcggccgctggatcctctag 2580 TAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATC EGFP reporter(2588,3304)>>> | XhoI ORF_2 rf(2)(2588,3307)>>> | | 2581 actcgagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcga 2640 TGAGCTCTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCT 2641 gctggacggcgacgtaaacggccacaggttcagcgtgtccggcgagggcgagggcgatgc 2700 CGACCTGCCGCTGCATTTGCCGGTGTCCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACG 2701 cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctg 2760 GTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGAC 2761 gcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgacca 2820 CGGGTGGGAGCACTGGTGGGACTGGACCCCGCACGTCACGAAGTCGGCGATGGGGCTGGT 2821 catgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgtac 2880 GTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCATG 2881 catcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcga 2940 GTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCT 2941 caccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcct 3000 GTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGA 3001 ggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca 3060 CCCCGTGTTCGACCTCATGTTGATGTAGTCGGTGTTGCAGATATAGTGGCGGCTGTTCGT 3061 gaagaacggcatcaaggcccacttcaagatccgccacaacatcgaggacggcagcgtgca 3120 CTTCTTGCCGTAGTTCCGGGTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGT 3121 gctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga 3180 CGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCT 3181 caaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatca 3240 GTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGT 3241 catggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta 3300 GTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACAT SV40 prom(3357,3625)>>> | 3301 caagtaatcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtg 3360 GTTCATTAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCAC 3361 tggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc 3420 ACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAG 3421 agcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgca 3480 TCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGT SV40 origin(3524,3601)>>> | 3481 tctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactcc 3540 AGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGG ORF_3 rf(3)(3591,4259)>>> | 3541 gcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggc 3600 CGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCG SfiI | 3601 cgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcca 3660 GCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGT puro marker(3660,4259)>>> | 3661 tgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtac 3720 ACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATG 3721 gcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggacc 3780 CGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGG 3781 gccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgaca 3840 CGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGT 3841 tcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggaga 3900 AGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCT 3901 gcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggtt 3960 CGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAA 3961 cccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagc 4020 GGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCG 4021 ccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggca 4080 GGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGT 4081 gcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctgg 4140 CGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACC 4141 agacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccg 4200 TCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGC 4201 acgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaa 4260 TGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTT 4261 cgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattctta 4320 GCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAAT 4321 actatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcta 4380 TGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGAT 4381 ttgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctcttt 4440 AACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAA 4441 atgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacg 4500 TACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGC 4501 caacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctt 4560 GTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAA 4561 tccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacag 4620 AGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTC 4621 gggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttc 4680 CCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAG 4681 catggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcc 4740 GTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGG 4741 cttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctc 4800 GAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAG 4801 ttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcc 4860 AAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGG delta_U3 other(4869,4921)>>> | 4861 tgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtac 4920 ACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATG HIV-1_5_LTR other(4922,5102)>>> | 4921 tgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaaccc 4980 ACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGG 4981 actgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgtt 5040 TGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAA 5041 gtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctag 5100 CACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATC 5101 cagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatc 5160 GTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAG 5161 agagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcat 5220 TCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTA 5221 cacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaact 5280 GTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGA 5281 catcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaa 5340 GTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTT 5341 tggcattatgggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaaa 5400 ACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTT 5401 taccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctca 5460 ATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGT 5461 ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcgg 5520 GACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCC 5521 taatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcc 5580 ATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGG pBR322 origin(5609,6228)<<< | 5581 agcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcc 5640 TCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGG 5641 cccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggac 5700 GGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTG 5701 tataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc 5760 ATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGG 5761 tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcata 5820 ACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTAT 5821 gctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgc 5880 CGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACG 5881 acgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcca 5940 TGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGT 5941 acccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagag 6000 TGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTC 6001 cgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacacta 6060 GCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGAT 6061 gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttg 6120 CTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAAC 6121 gtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagc 6180 CATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCG 6181 agcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggt 6240 TCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCA 6241 ctgacgctcagtggaacgaaaactcacgttaagggattttggccatgagattatcaaaaa 6300 GACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTT 6301 ggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgacccc 6360 CCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGG 6361 ggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagc 6420 CCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCG 6421 aggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaa 6480 TCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTT NEOKAN prom(6486,6535)>>> | 6481 gcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaa 6540 CGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATT BclI | 6541 actggatggctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaag 6600 TGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTC NTP_II marker(6627,7415)>>> | ORF_4 rf(3)(6624,7418)>>> | | 6601 agacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccgg 6660 TCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCC 6661 ccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctg 6720 GGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGAC 6721 atgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacc 6780 TACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGG 6781 tgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacga 6840 ACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCT 6841 cgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgc 6900 GCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACG 6901 tattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaag 6960 ATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTC 6961 tatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccat 7020 ATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTA 7021 tcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttg 7080 AGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAAC 7081 tcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcca 7140 AGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGT 7141 ggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgct 7200 CCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGA 7201 tgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgg 7260 ACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACC 7261 gtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttg 7320 CACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAAC 7321 gcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagc 7380 CGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCG 7381 gcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaa 7440 CGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATT 7441 atcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaa 7500 TAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTT f1 origin(7523,7829)<<< | 7501 tagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaac 7560 ATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTG 7561 gtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaa 7620 CACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTT 7621 ccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccct 7680 GGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGA 7681 aaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaa 7740 TTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTT 7741 gggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgc 7800 CCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCG 7801 gtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattca 7860 CATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGT LITR other(7884,7985)>>> | RITR other(7884,7985)<<< | 7861 ggatcgaattaattcttaattaacatcatcaataatataccttttttggattgaagccaa 7920 CCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGTT 7921 tatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtga 7980 ATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACT 7981 cgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacgga 8040 GCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCCT Encap other(8065,8213)>>> | 8041 tgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcg 8100 ACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGC 8101 cggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttc 8160 GCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAG 8161 gcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgt 8220 CGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGCA 8221 aatactctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtt 8280 TTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAA 8281 ccgcgcacatttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcga 8340 GGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGCT 8341 aaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaac 8400 TTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTG 8401 atgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacg 8460 TACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGC 8461 at 8462 TA
LOCUS dna 8462 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 2005..2010 /dbxref="REBASE:NdeI" Promoter 2279..2360 /gene="CMV prom" ORF 2354..3409 /sequence="ORF_5 rf(5)" Regulatory_Seq 2416..2455 /gene="tetO reg" misc_binding 2462..2467 /dbxref="REBASE:SmaI" misc_binding 2462..2467 /dbxref="REBASE:XmaI" misc_binding 2468..2473 /dbxref="REBASE:ClaI" misc_binding 2492..2497 /dbxref="REBASE:KpnI" misc_binding 2511..2516 /dbxref="REBASE:PstI" misc_binding 2519..2524 /dbxref="REBASE:EcoRI" misc_binding 2537..2542 /dbxref="REBASE:StuI" misc_binding 2543..2548 /dbxref="REBASE:HincII" misc_binding 2549..2554 /dbxref="REBASE:EcoRV" misc_binding 2555..2560 /dbxref="REBASE:ApaI" misc_binding 2561..2568 /dbxref="REBASE:NotI" misc_binding 2570..2575 /dbxref="REBASE:BamHI" misc_binding 2576..2581 /dbxref="REBASE:XbaI" misc_binding 2582..2587 /dbxref="REBASE:XhoI" Reporter 2588..3304 /gene="EGFP reporter" ORF 2588..3307 /sequence="ORF_2 rf(2)" Promoter 3357..3625 /gene="SV40 prom" Rep_Origin 3524..3601 /gene="SV40 origin" ORF 3591..4259 /sequence="ORF_3 rf(3)" misc_binding 3604..3616 /dbxref="REBASE:SfiI" Marker 3660..4259 /gene="puro marker" Other Gene 4869..4921 /gene="delta_U3 other" Other Gene 4922..5102 /gene="HIV-1_5_LTR other" Rep_Origin 5609..6228 /gene="pBR322 origin" Promoter 6486..6535 /gene="NEOKAN prom" misc_binding 6593..6598 /dbxref="REBASE:BclI" ORF 6624..7418 /sequence="ORF_4 rf(3)" Marker 6627..7415 /gene="NTP_II marker" Rep_Origin 7523..7829 /gene="f1 origin" Other Gene 7884..7985 /gene="RITR other" Other Gene 7884..7985 /gene="LITR other" Other Gene 8065..8213 /gene="Encap other" BASE COUNT 2107 a 2148 c 2324 g 1883 t 0 others