pLenti-His
| Vector Name | pLenti-His |
| VectorType | Lentiviral vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgt
agtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttgg
tgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt
aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaat
acggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt
agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta
gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagcc
agtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttg
accgacaattaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa
ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacggg
tctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacg
ggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc
ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtc
agtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcag
gactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag
gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggtta
aggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcc
tggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac
ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagcttta
gacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcgccgctgatcttcagacctggaggaggaga
tatgagggacaattgagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc
aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaa
gcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaat
ttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct
ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctg
tgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaa
attaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt
ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatga
tagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacca
ttatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga
cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagttccgcgttacataacttacgg
taaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcca
atagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat
gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggact
ttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgt
ggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca
acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctata
taagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgg
ctagcgtttaaacttaagcttggtaccggatccactagtgaattctctagagcggccgcaccgcggagtactgggccccc
taggctcgagcatcatcaccatcaccattgatgatccggctgtggaatgtgtgtcagttagggtgtggaaagtccccagg
ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccag
caggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgccccta
actccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggc
ctctgagctattccagaagtagtgaggaggcttttttggaggcctagcgaccagatctaatgcgtGCTAGCACGCATTAG
ATCGGTCGCTAGCGACCAGATCTAATGCGTGCTAGCGACCAGATCTAATGCGTGCTAGGcttttgcaaaaagcttgggct
gcaggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttc
tccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttcc
ggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca
gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactg
gctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctg
atgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagca
cgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt
cgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatgg
tggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggct
acccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattc
gcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgatatctagcgaccagatct
aattgcgtgcaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaac
gaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaacta
gggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctgg
taactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagt
atttataacttgcaaagaaatgaatatcagagagtgagaggaacttgggcctgaccagatctaatgcgtaggccgtttaa
accgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccgggcgcgatcgctgcccctcccccgt
gccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagta
ggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggg
gatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcatctatgtcgggtgcggagaaagagg
taatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatg
cgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggc
gagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagca
aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagca
tcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagct
ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctt
tctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgt
tcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag
cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggc
tacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaag
aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagatta
tcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttg
gtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac
tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgc
ctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaa
aaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctact
gggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagac
tgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagt
aaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgt
ttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggca
caacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccga
cctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcag
ctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatct
caccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgccc
attcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctgg
acgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtc
gtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggct
gggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgacc
gcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctga
attttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaag
aatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaa
gggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccg
taaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcg
cgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatata
cctt
LOCUS dna 8100 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_1 rf(2)"
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
misc_binding 3062..3067
/dbxref="REBASE:KpnI"
misc_binding 3068..3073
/dbxref="REBASE:BamHI"
misc_binding 3080..3085
/dbxref="REBASE:EcoRI"
misc_binding 3086..3091
/dbxref="REBASE:XbaI"
misc_binding 3092..3099
/dbxref="REBASE:NotI"
misc_binding 3113..3118
/dbxref="REBASE:ApaI"
misc_binding 3125..3130
/dbxref="REBASE:XhoI"
Tag 3131..3148
/gene="6xHis tag"
Regulatory_Seq 3164..3400
/gene="SV40ER reg"
Promoter 3187..3455
/gene="SV40 prom"
Rep_Origin 3354..3431
/gene="SV40 origin"
misc_binding 3434..3446
/dbxref="REBASE:SfiI"
misc_binding 3485..3490
/dbxref="REBASE:StuI"
ORF 3585..4379
/sequence="ORF_2 rf(3)"
Marker 3588..4376
/gene="NTP_II marker"
misc_binding 4397..4402
/dbxref="REBASE:EcoRV"
Other Gene 4473..4525
/gene="delta_U3 other"
Other Gene 4526..4706
/gene="HIV-1_5_LTR other"
misc_binding 4868..4873
/dbxref="REBASE:SmaI"
misc_binding 4868..4873
/dbxref="REBASE:XmaI"
Rep_Origin 5412..6031
/gene="pBR322 origin"
Promoter 6684..6733
/gene="NEOKAN prom"
ORF 6822..7616
/sequence="ORF_3 rf(3)"
Marker 6825..7613
/gene="NTP_II marker"
Rep_Origin 7721..8026
/gene="f1 origin"
BASE COUNT 2064 a 1913 c 2233 g 1890 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct
3061 tggtaccgga tccactagtg aattctctag agcggccgca ccgcggagta ctgggccccc
3121 taggctcgag catcatcacc atcaccattg atgatgatcc ccggctgtgg aatgtgtgtc
3181 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3241 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3301 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
3361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
3421 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
3481 ttggaggcct agtgaccaga tctaatgcgt aggccctttt gcaaaaagct tgggctgcag
3541 gtcgaggcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
3601 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
3661 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
3721 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
3781 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
3841 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
3901 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
3961 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4021 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4081 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
4141 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4201 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4261 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
4321 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
4381 cgggactctg gggttcgata tctagcgacc agatctaatt gcgtgcaagg cagctgtaga
4441 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
4501 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
4561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
4621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
4681 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
4741 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgggcctga ccagatctaa
4801 tgcgtaggcc gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
4861 gttgttgccc gggcgcgatc gctgcccctc ccccgtgcct tccttgaccc tggaaggtgc
4921 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
4981 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
5041 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcag
5101 atcgatctgc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
5161 atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta
5221 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
5341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
5401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
5701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
5821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
5881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
5941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
6001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
6061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
6121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
6181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
6241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
6301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
6421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
6481 cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc
6541 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
6601 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
6661 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
6721 ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg
6781 gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
6841 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
6901 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
6961 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
7021 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
7081 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
7141 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
7201 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
7261 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
7321 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
7381 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
7441 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
7501 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
7561 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt
7621 tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc
7681 ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag
7741 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
7801 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa
7861 gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
7921 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
7981 gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg
8041 cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtct 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAATTACATCAGA
661 tatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa 720
ATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTT
721 ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgcctta 780
CCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGCACGGAAT
781 ttaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgc 840
AATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGGCGTAACG
HIV-1_5_LTR other(880,1060)>>>
|
841 agagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccag 900
TCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACCAATCTGGTC
901 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 960
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG
961 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 1020
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT
1021 tccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggact 1080
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGA
1081 tgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgc 1140
ACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCG
HIV-1_psi_pack other(1171,1215)>>>
|
1141 gcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag 1200
CGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTC
1201 gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgc 1260
CGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCG
NruI
|
1261 gatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatag 1320
CTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATATC
1321 tatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcag 1380
ATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTC
1381 aaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac 1440
TTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTG
1441 ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataa 1500
AATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCTCTATT
1501 aagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccg 1560
TTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGC
1561 cacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaag 1620
GTGTCGTTCGCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACTCTTC
1621 tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc 1680
ACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGTGGTTCCG
RRE reg(1723,1956)>>>
|
1681 aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgg 1740
TTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGAACC
ORF_1 rf(2)(1766,2449)>>>
|
1741 gttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggc 1800
CAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCG
1801 cagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc 1860
GTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGATAACTCCG
1861 gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct 1920
CGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTTCTTAGGA
1921 ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaa 1980
CCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTT
1981 actcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaaca 2040
TGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAGACCTTGT
2041 gatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt 2100
CTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTGTTCGAA
2101 aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt 2160
TTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAA
2161 ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggta 2220
CCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGACACCAT
2221 tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgt 2280
ATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAAACGACA
2281 actttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct 2340
TGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGA
2341 cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga 2400
GGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCTCTCTCT
2401 cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagtt 2460
GTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTATTCGAACCCTCAA
2461 ccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgccc 2520
GGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGG
2521 attgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacg 2580
TAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGC
NdeI
|
2581 tcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat 2640
AGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATA
2641 gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgccca 2700
CGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGT
2701 gtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctat 2760
CATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATA
2761 taccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacg 2820
ATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGC
2821 gggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca 2880
CCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGT
CMV prom(2911,2992)>>>
|
2881 acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcg 2940
TGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGC
2941 tgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggag 3000
ACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTC
3001 acgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagct 3060
TGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCGATCGCAAATTTGAATTCGA
BamHI
|
KpnI EcoRI XbaI NotI ApaI
| | | | | |
3061 tggtaccggatccactagtgaattctctagagcggccgcaccgcggagtactgggccccc 3120
ACCATGGCCTAGGTGATCACTTAAGAGATCTCGCCGGCGTGGCGCCTCATGACCCGGGGG
XhoI 6xHis tag(3131,3148)>>> SV40ER reg(3164,3400)<<<
| | |
3121 taggctcgagcatcatcaccatcaccattgatgatgatccccggctgtggaatgtgtgtc 3180
ATCCGAGCTCGTAGTAGTGGTAGTGGTAACTACTACTAGGGGCCGACACCTTACACACAG
SV40 prom(3187,3455)>>>
|
3181 agttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatc 3240
TCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAG
3241 tcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgc 3300
AGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACG
SV40 origin(3354,3431)>>>
|
3301 aaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgc 3360
TTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCG
3361 ccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttattt 3420
GGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAA
SfiI
|
3421 atgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggctttt 3480
TACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAA
StuI
|
3481 ttggaggcctagtgaccagatctaatgcgtaggcccttttgcaaaaagcttgggctgcag 3540
AACCTCCGGATCACTGGTCTAGATTACGCATCCGGGAAAACGTTTTTCGAACCCGACGTC
NTP_II marker(3588,4376)>>>
|
ORF_2 rf(3)(3585,4379)>>>
| |
3541 gtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatg 3600
CAGCTCCGCCTAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTAC
3601 gattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcac 3660
CTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTG
3661 aacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccgg 3720
TTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCC
3721 ttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgc 3780
AAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCG
3781 ggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 3840
CCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGAC
3841 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctc 3900
TTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAG
3901 accttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgc 3960
TGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCG
3961 ttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgta 4020
AACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCAT
4021 ctcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcg 4080
GAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGC
4081 cgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcg 4140
GCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGCAGC
4141 tgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggat 4200
ACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTA
4201 tcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctaccc 4260
AGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGG
4261 gtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggta 4320
CACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCAT
4321 tcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgag 4380
AGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTC
EcoRV
|
4381 cgggactctggggttcgatatctagcgaccagatctaattgcgtgcaaggcagctgtaga 4440
GCCCTGAGACCCCAAGCTATAGATCGCTGGTCTAGATTAACGCACGTTCCGTCGACATCT
delta_U3 other(4473,4525)>>>
|
4441 tcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaag 4500
AGAATCGGTGAAAAATTTTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTTC
HIV-1_5_LTR other(4526,4706)>>>
|
4501 acaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctggga 4560
TGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCT
4561 gctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgct 4620
CGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGA
4621 tcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccctt 4680
AGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAA
4681 ttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtattt 4740
AATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAA
4741 ataacttgcaaagaaatgaatatcagagagtgagaggaacttgggcctgaccagatctaa 4800
TATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACCCGGACTGGTCTAGATT
4801 tgcgtaggccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatct 4860
ACGCATCCGGCAAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGA
SmaI
|
XmaI
| |
4861 gttgttgcccgggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgc 4920
CAACAACGGGCCCGCGCTAGCGACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACG
4921 cactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtg 4980
GTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCAC
4981 tcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaa 5040
AGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTT
5041 tagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcag 5100
ATCGTCCGTACGACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTC
5101 atcgatctgcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtatt 5160
TAGCTAGACGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAA
5161 atgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgta 5220
TACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCAT
5221 aggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcg 5280
TCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGC
5281 gtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaca 5340
CAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGT
5341 gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaac 5400
CTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTG
pBR322 origin(5412,6031)<<<
|
5401 cgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcac 5460
GCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTG
5461 aaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcg 5520
TTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGC
5521 tttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatac 5580
AAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATG
5581 ctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtat 5640
GACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATA
5641 ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcag 5700
GAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTC
5701 cccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgac 5760
GGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTG
5761 ttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggt 5820
AATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCA
5821 gctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggt 5880
CGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCA
5881 atctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc 5940
TAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCG
5941 aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcaga 6000
TTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCT
6001 aaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaac 6060
TTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTG
6061 gaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatc 6120
CTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAG
6121 cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct 6180
GAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGA
6181 gacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttca 6240
CTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGT
6241 tccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatct 6300
AGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGA
6301 ggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca 6360
CCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGT
6361 ataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcc 6420
TATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGG
6421 atccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttg 6480
TAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAAC
6481 cgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgc 6540
GCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCG
6541 agaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacg 6600
TCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGC
6601 caagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactggg 6660
GTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCC
NEOKAN prom(6684,6733)>>>
|
6661 cggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttg 6720
GCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAAC
6721 ggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggg 6780
CCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCC
NTP_II marker(6825,7613)>>>
|
ORF_3 rf(3)(6822,7616)>>>
| |
6781 gatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggat 6840
CTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTA
6841 tgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaac 6900
ACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTG
6901 agacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttc 6960
TCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAG
6961 tttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggc 7020
AAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCG
7021 tatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaag 7080
ATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTC
7081 cgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacc 7140
GCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGG
7141 ttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttg 7200
AACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAAC
7201 atccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactc 7260
TAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAG
7261 ggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgc 7320
CCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCG
7321 cagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtga 7380
GTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACT
7381 cccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattca 7440
GGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGT
7441 tcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtg 7500
AGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCAC
7501 atattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcg 7560
TATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGC
7561 ccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattt 7620
GGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAA
7621 tgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatc 7680
ACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAG
f1 origin(7721,8026)<<<
|
7681 ccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaag 7740
GGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTC
7741 agtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggc 7800
TCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCG
7801 gatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaa 7860
CTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTT
7861 gctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcg 7920
CGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGC
7921 aacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagt 7980
TTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCA
7981 gtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcg 8040
CATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGC
8041 cgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 8100
GCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
5'-LTR
Start: 670 End: 1151
CMV Promoter
Start: 2451 End: 3020
Neomycin
Start: 3585 End: 4379
3'-LTR
Start: 4501 End: 4790
Kanamycin
Start: 6822 End: 7613