pLenti-HOXB8-RFP
| Vector Name | pLenti-HOXB8-RFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact 3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct 3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct 3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc 3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct 3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg 3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga 3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc 3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta 3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga 3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag 3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg 3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct 3841 tgtacaaagt ggttgatatc tgactcgagt gaatgaatga ggtgtggaaa gtccccaggc 3901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3961 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4021 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4141 tctgagctat tccagaagta gtgaggaggc ttttttggag gccactagtg tcgagaaaac 4201 tagtcgccac catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg 4261 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg 4321 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg 4381 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca 4441 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat 4501 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca 4561 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga 4621 aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag 4681 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga 4741 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg 4801 acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg 4861 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagagaa ttcggaagcg 4921 ggcagtgcac caactacgcc ctgctgaagc tggccggcga cgtggagagc aaccccggcc 4981 ccggatccat ggccaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 5041 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 5101 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 5161 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 5221 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggctcg cgcatggccg 5281 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 5341 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 5401 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gctggggtgc 5461 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 5521 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 5581 ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga 5641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 6001 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6121 ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc 6181 cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct 6241 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6421 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6481 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6601 tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa 6661 gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc 6721 ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 6781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag 8041 atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc 8101 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt 8161 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg 8221 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc 8281 tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga 8341 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 8401 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 8461 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 8521 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 8581 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 8641 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 8701 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 8761 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 8821 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc 8881 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 8941 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 9001 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 9061 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 9121 cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt 9181 taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg 9241 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 9301 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc 9361 aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg 9421 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 9481 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 9541 cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc 9601 ttaattaaca tcatcaataa tatacctt
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_3 rf(3)(2901,3863)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGAGCTCTTATTTCGTCAACT 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACTCGAGAATAAAGCAGTTGA 3121 CACTGTTCTCCAAATACAAAACCGGGGAGTCCCTGCGCCCCAATTATTATGACTGCGGCT 3180 GTGACAAGAGGTTTATGTTTTGGCCCCTCAGGGACGCGGGGTTAATAATACTGACGCCGA 3181 TCGCCCAGGACCTGGGCGGCCGACCCACCGTGGTGTACGGTCCCAGCAGCGGCGGCAGCT 3240 AGCGGGTCCTGGACCCGCCGGCTGGGTGGCACCACATGCCAGGGTCGTCGCCGCCGTCGA 3241 TCCAGCACCCGTCGCAAATCCAGGAGTTCTACCACGGGCCGTCGTCGCTGTCCACGGCTC 3300 AGGTCGTGGGCAGCGTTTAGGTCCTCAAGATGGTGCCCGGCAGCAGCGACAGGTGCCGAG 3301 CCTACCAGCAGAACCCGTGCGCCGTGGCGTGCCACGGGGACCCCGGCAATTTCTACGGCT 3360 GGATGGTCGTCTTGGGCACGCGGCACCGCACGGTGCCCCTGGGGCCGTTAAAGATGCCGA 3361 ACGACCCGCTGCAACGCCAGAGCCTATTCGGTGCGCAGGATCCAGACCTGGTGCAGTACG 3420 TGCTGGGCGACGTTGCGGTCTCGGATAAGCCACGCGTCCTAGGTCTGGACCACGTCATGC 3421 CAGACTGCAAGCTTGCCGCCGCCAGCGGCCTGGGCGAGGAGGCCGAGGGCTCCGAGCAGA 3480 GTCTGACGTTCGAACGGCGGCGGTCGCCGGACCCGCTCCTCCGGCTCCCGAGGCTCGTCT 3481 GCCCGTCGCCCACACAGCTCTTCCCCTGGATGCGCCCGCAAGCAGCCGCCGGACGCAGGC 3540 CGGGCAGCGGGTGTGTCGAGAAGGGGACCTACGCGGGCGTTCGTCGGCGGCCTGCGTCCG 3541 GAGGCCGACAGACCTACAGCCGCTACCAGACCCTGGAGCTGGAGAAGGAGTTCCTATTTA 3600 CTCCGGCTGTCTGGATGTCGGCGATGGTCTGGGACCTCGACCTCTTCCTCAAGGATAAAT 3601 ATCCCTATCTGACTCGTAAGCGGCGAATCGAGGTATCGCACGCCCTGGGACTGACAGAGA 3660 TAGGGATAGACTGAGCATTCGCCGCTTAGCTCCATAGCGTGCGGGACCCTGACTGTCTCT 3661 GACAGGTCAAAATCTGGTTCCAGAACCGGAGGATGAAGTGGAAAAAAGAGAACAACAAAG 3720 CTGTCCAGTTTTAGACCAAGGTCTTGGCCTCCTACTTCACCTTTTTTCTCTTGTTGTTTC 3721 ACAAGTTCCCCAGCAGCAAATGCGAGCAGGAGGAGCTGGAGAAACAGAAGCTGGAGCGGG 3780 TGTTCAAGGGGTCGTCGTTTACGCTCGTCCTCCTCGACCTCTTTGTCTTCGACCTCGCCC ApaI | 3781 CCCCAGAGGCGGCGGACGAGGGCGACGCGCAGAAGGGCGACAAGAAGtgcccaactttct 3840 GGGGTCTCCGCCGCCTGCTCCCGCTGCGCGTCTTCCCGCTGTTCTTCACGGGTTGAAAGA XhoI SV40 prom(3881,4149)>>> | | 3841 tgtacaaagtggttgatatctgactcgagtgaatgaatgaggtgtggaaagtccccaggc 3900 ACATGTTTCACCAACTATAGACTGAGCTCACTTACTTACTCCACACCTTTCAGGGGTCCG 3901 tccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtgga 3960 AGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCT 3961 aagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagca 4020 TTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGT SV40 origin(4048,4125)>>> | 4021 accatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccat 4080 TGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTA SfiI | 4081 tctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcc 4140 AGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGG 4141 tctgagctattccagaagtagtgaggaggcttttttggaggccactagtgtcgagaaaac 4200 AGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTGATCACAGCTCTTTTG ORF_4 rf(3)(4212,5591)>>> | 4201 tagtcgccaccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatgg 4260 ATCAGCGGTGGTACCACTCGCTCGACTAATTCCTCTTGTACGTGTACTTCGACATGTACC 4261 agggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacg 4320 TCCCGTGGCACTTGTTGGTGGTGAAGTTCACGTGTAGGCTCCCGCTTCCGTTCGGGATGC 4321 agggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcg 4380 TCCCGTGGGTCTGGTACTCTTAGTTCCGCCAGCTCCCGCCGGGAGAGGGGAAGCGGAAGC 4381 acatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggca 4440 TGTAGGACCGATGGTCGAAGTACATGCCGTCGTTTTGGAAGTAGTTGGTGTGGGTCCCGT 4441 tccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacat 4500 AGGGGCTGAAGAAATTCGTCAGGAAGGGGCTCCCGAAGTGTACCCTCTCTCAGTGGTGTA 4501 acgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctca 4560 TGCTTCTGCCCCCGCACGACTGGCGATGGGTCCTGTGGTCGGAGGTCCTGCCGACGGAGT 4561 tctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaaga 4620 AGATGTTGCAGTTCTAGTCTCCCCACTTGAAGGGTAGGTTGCCGGGACACTACGTCTTCT 4621 aaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaag 4680 TTTGTGAGCCGACCCTCCGGAGGTGGCTCTGGGACATGGGGCGACTGCCGCCGGACCTTC 4681 gcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaaga 4740 CGTCTCGGCTGTACCGGGACTTCGAGCACCCGCCCCCGGTGGACTAGACGTTGAACTTCT 4741 ccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtgg 4800 GGTGTATGTCTAGGTTCTTTGGGCGATTCTTGGAGTTCTACGGGCCGCAGATGATACACC 4801 acagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgagg 4860 TGTCTTCTGACCTTTCTTAGTTCCTCCGGCTGTTTCTCTGGATGCAGCTCGTCGTGCTCC EcoRI | 4861 tggctgtggccagatactgcgacctccctagcaaactggggcacagagaattcggaagcg 4920 ACCGACACCGGTCTATGACGCTGGAGGGATCGTTTGACCCCGTGTCTCTTAAGCCTTCGC 4921 ggcagtgcaccaactacgccctgctgaagctggccggcgacgtggagagcaaccccggcc 4980 CCGTCACGTGGTTGATGCGGGACGACTTCGACCGGCCGCTGCACCTCTCGTTGGGGCCGG puro marker(4995,5591)>>> | 4981 ccggatccatggccaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcc 5040 GGCCTAGGTACCGGTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGG SmaI | XmaI | | 5041 cccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccg 5100 GGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGC 5101 tcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcg 5160 AGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGC 5161 tcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctgga 5220 AGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCT 5221 ccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggctcgcgcatggccg 5280 GGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGAGCGCGTACCGGC 5281 agttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcacc 5340 TCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGG 5341 ggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggca 5400 CCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGT 5401 agggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgctggggtgc 5460 TCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGACCCCACG 5461 ccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttca 5520 GGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGT 5521 ccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagc 5580 GGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCG 5581 ccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattga 5640 GGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACT 5641 ctggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctt 5700 GACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAA 5701 tgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggt 5760 ACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCA 5761 tgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactg 5820 ACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGAC 5821 tgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccg 5880 ACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGC 5881 ggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgccc 5940 CCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGG 5941 gctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagc 6000 CGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCG 6001 tgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtcct 6060 ACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGA 6061 tctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccgg 6120 AGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCC 6121 ctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggc 6180 GAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCG delta_U3 other(6201,6253)>>> | 6181 cgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgct 6240 GCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGA HIV-1_5_LTR other(6254,6434)>>> | 6241 ttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggcta 6300 AAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGAT 6301 actagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtg 6360 TGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACAC 6361 tgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtg 6420 ACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACAC 6421 gaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaa 6480 CTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTT 6481 gaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaata 6540 CTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTAT 6541 aagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtgg 6600 TTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACC 6601 tttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaa 6660 AAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTT 6661 gaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatc 6720 CTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAG 6721 ccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgc 6780 GGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCG 6781 ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctca 6840 AAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGT 6841 ctcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtg 6900 GAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACAC pBR322 origin(6940,7559)<<< | 6901 agcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca 6960 TCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGT 6961 taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaa 7020 ATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTT 7021 cccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcc 7080 GGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGG 7081 tgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc 7140 ACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCG 7141 gctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagct 7200 CGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGA 7201 gggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg 7260 CCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGC 7261 tcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacag 7320 AGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTC 7321 gattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaacta 7380 CTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGAT 7381 cggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgg 7440 GCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCC 7441 aaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttt 7500 TTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAA 7501 tgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt 7560 ACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAA 7561 ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgag 7620 AAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTC 7621 attatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaat 7680 TAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTA 7681 ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacc 7740 GATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGG 7741 tatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat 7800 ATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTA 7801 aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaccc 7860 TTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGG 7861 acgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcag 7920 TGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTC 7921 aagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctag 7980 TTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATC 7981 agtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctag 8040 TCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATC 8041 atccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagc 8100 TAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCG 8101 tactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagt 8160 ATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCA NEOKAN prom(8212,8261)>>> | 8161 gggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattg 8220 CCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAAC 8221 ccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttc 8280 GGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAG BclI | 8281 tcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgagga 8340 AGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCT NTP_II marker(8353,9141)>>> | ORF_2 rf(1)(8350,9144)>>> | | 8341 tcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggag 8400 AGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTC 8401 aggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttc 8460 TCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAG 8461 cggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctg 8520 GCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGAC 8521 aatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgc 8580 TTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACG 8581 gcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtg 8640 CGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCAC 8641 ccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggct 8700 GGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGA 8701 gatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcg 8760 CTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGC 8761 aaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgat 8820 TTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTA 8821 ctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagc 8880 GACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCG 8881 atgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatg 8940 TACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTAC 8941 gtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgc 9000 CACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCG 9001 tatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggct 9060 ATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGA 9061 gaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctat 9120 CTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATA 9121 cgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttt 9180 GCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAA 9181 taaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagg 9240 ATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCC f1 origin(9249,9554)<<< | 9241 gttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgt 9300 CAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCA 9301 caaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatc 9360 GTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAG 9361 aagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccg 9420 TTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGC 9421 atttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaa 9480 TAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTT 9481 aggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacc 9540 TCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGG 9541 cgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattc 9600 GCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAG 9601 ttaattaacatcatcaataatatacctt 9628 AATTAATTGTAGTAGTTATTATATGGAA
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
LOCUS dna 9628 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3863 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3778..3783 /dbxref="REBASE:ApaI" misc_binding 3864..3869 /dbxref="REBASE:XhoI" Promoter 3881..4149 /gene="SV40 prom" Rep_Origin 4048..4125 /gene="SV40 origin" misc_binding 4128..4140 /dbxref="REBASE:SfiI" ORF 4212..5591 /sequence="ORF_4 rf(3)" misc_binding 4908..4913 /dbxref="REBASE:EcoRI" Marker 4995..5591 /gene="puro marker" misc_binding 5041..5046 /dbxref="REBASE:SmaI" misc_binding 5041..5046 /dbxref="REBASE:XmaI" Other Gene 6201..6253 /gene="delta_U3 other" Other Gene 6254..6434 /gene="HIV-1_5_LTR other" Rep_Origin 6940..7559 /gene="pBR322 origin" Promoter 8212..8261 /gene="NEOKAN prom" misc_binding 8319..8324 /dbxref="REBASE:BclI" ORF 8350..9144 /sequence="ORF_2 rf(1)" Marker 8353..9141 /gene="NTP_II marker" Rep_Origin 9249..9554 /gene="f1 origin" BASE COUNT 2431 a 2460 c 2629 g 2108 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact 3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct 3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct 3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc 3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct 3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg 3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga 3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc 3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta 3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga 3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag 3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg 3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct 3841 tgtacaaagt ggttgatatc tgactcgagt gaatgaatga ggtgtggaaa gtccccaggc 3901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3961 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4021 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4141 tctgagctat tccagaagta gtgaggaggc ttttttggag gccactagtg tcgagaaaac 4201 tagtcgccac catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg 4261 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg 4321 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg 4381 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca 4441 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat 4501 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca 4561 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga 4621 aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag 4681 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga 4741 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg 4801 acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg 4861 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagagaa ttcggaagcg 4921 ggcagtgcac caactacgcc ctgctgaagc tggccggcga cgtggagagc aaccccggcc 4981 ccggatccat ggccaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 5041 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 5101 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 5161 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 5221 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggctcg cgcatggccg 5281 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 5341 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 5401 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gctggggtgc 5461 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 5521 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 5581 ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga 5641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 6001 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6121 ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc 6181 cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct 6241 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6421 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6481 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6601 tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa 6661 gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc 6721 ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 6781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag 8041 atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc 8101 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt 8161 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg 8221 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc 8281 tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga 8341 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 8401 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 8461 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 8521 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 8581 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 8641 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 8701 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 8761 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 8821 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc 8881 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 8941 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 9001 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 9061 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 9121 cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt 9181 taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg 9241 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 9301 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc 9361 aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg 9421 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 9481 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 9541 cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc 9601 ttaattaaca tcatcaataa tatacctt //
LOCUS dna 9628 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3863 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3778..3783 /dbxref="REBASE:ApaI" misc_binding 3864..3869 /dbxref="REBASE:XhoI" Promoter 3881..4149 /gene="SV40 prom" Rep_Origin 4048..4125 /gene="SV40 origin" misc_binding 4128..4140 /dbxref="REBASE:SfiI" ORF 4212..5591 /sequence="ORF_4 rf(3)" misc_binding 4908..4913 /dbxref="REBASE:EcoRI" Marker 4995..5591 /gene="puro marker" misc_binding 5041..5046 /dbxref="REBASE:SmaI" misc_binding 5041..5046 /dbxref="REBASE:XmaI" Other Gene 6201..6253 /gene="delta_U3 other" Other Gene 6254..6434 /gene="HIV-1_5_LTR other" Rep_Origin 6940..7559 /gene="pBR322 origin" Promoter 8212..8261 /gene="NEOKAN prom" misc_binding 8319..8324 /dbxref="REBASE:BclI" ORF 8350..9144 /sequence="ORF_2 rf(1)" Marker 8353..9141 /gene="NTP_II marker" Rep_Origin 9249..9554 /gene="f1 origin" BASE COUNT 2431 a 2460 c 2629 g 2108 t 0 others