pLenti-HOXB8-GFP
| Vector Name | pLenti-HOXB8-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact 3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct 3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct 3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc 3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct 3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg 3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga 3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc 3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta 3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga 3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag 3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg 3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct 3841 tgtacaaagt ggttgatatc tgaggatcct ctagaggtgt ggaaagtccc caggctcccc 3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4141 gctattccag aagtagtgag gaggcttttt tggaggcctg tcgaggccac catggagagc 4201 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 4261 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 4321 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 4381 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 4441 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 4501 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 4561 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 4621 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 4681 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 4741 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 4801 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 4861 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 4921 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 4981 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 5041 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 5101 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5161 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 5221 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5281 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 5341 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 5401 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5461 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5521 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5581 acccgcaagc ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt 5641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6001 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 6061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6121 ctgctgccgg ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct 6181 ccctttgggc cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac 6241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6421 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg 6661 tgcggagaaa gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg 6721 gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat 8041 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat 8101 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt 8161 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga 8221 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg 8281 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac 8341 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 8401 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 8461 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 8521 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 8581 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 8641 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 8701 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 8761 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 8821 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 8881 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 8941 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 9001 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 9061 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 9121 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca 9181 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga 9241 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 9301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 9361 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag 9421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 9481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 9541 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 9601 gaattaattc ttaattaaca tcatcaataa tatacctt
LOCUS dna 9638 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3863 /sequence="ORF_4 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3870..3875 /dbxref="REBASE:XbaI" Promoter 3876..4144 /gene="SV40 prom" Rep_Origin 4043..4120 /gene="SV40 origin" misc_binding 4123..4135 /dbxref="REBASE:SfiI" ORF 4192..5601 /sequence="ORF_2 rf(1)" misc_binding 4765..4770 /dbxref="REBASE:PstI" Marker 5002..5601 /gene="puro marker" Other Gene 6211..6263 /gene="delta_U3 other" Other Gene 6264..6444 /gene="HIV-1_5_LTR other" Rep_Origin 6950..7569 /gene="pBR322 origin" Promoter 8222..8271 /gene="NEOKAN prom" misc_binding 8329..8334 /dbxref="REBASE:BclI" ORF 8360..9154 /sequence="ORF_3 rf(2)" Marker 8363..9151 /gene="NTP_II marker" Rep_Origin 9259..9564 /gene="f1 origin" BASE COUNT 2385 a 2498 c 2647 g 2108 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact 3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct 3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct 3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc 3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct 3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg 3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga 3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc 3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta 3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga 3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag 3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg 3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct 3841 tgtacaaagt ggttgatatc tgaggatcct ctagaggtgt ggaaagtccc caggctcccc 3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4141 gctattccag aagtagtgag gaggcttttt tggaggcctg tcgaggccac catggagagc 4201 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 4261 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 4321 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 4381 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 4441 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 4501 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 4561 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 4621 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 4681 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 4741 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 4801 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 4861 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 4921 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 4981 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 5041 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 5101 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 5161 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 5221 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5281 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 5341 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 5401 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5461 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5521 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5581 acccgcaagc ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt 5641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6001 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 6061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6121 ctgctgccgg ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct 6181 ccctttgggc cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac 6241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6421 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg 6661 tgcggagaaa gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg 6721 gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat 8041 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat 8101 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt 8161 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga 8221 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg 8281 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac 8341 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 8401 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 8461 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 8521 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 8581 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 8641 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 8701 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 8761 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 8821 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 8881 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 8941 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 9001 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 9061 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 9121 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca 9181 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga 9241 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 9301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 9361 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag 9421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 9481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 9541 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 9601 gaattaattc ttaattaaca tcatcaataa tatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_4 rf(3)(2901,3863)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGAGCTCTTATTTCGTCAACT 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACTCGAGAATAAAGCAGTTGA 3121 CACTGTTCTCCAAATACAAAACCGGGGAGTCCCTGCGCCCCAATTATTATGACTGCGGCT 3180 GTGACAAGAGGTTTATGTTTTGGCCCCTCAGGGACGCGGGGTTAATAATACTGACGCCGA 3181 TCGCCCAGGACCTGGGCGGCCGACCCACCGTGGTGTACGGTCCCAGCAGCGGCGGCAGCT 3240 AGCGGGTCCTGGACCCGCCGGCTGGGTGGCACCACATGCCAGGGTCGTCGCCGCCGTCGA 3241 TCCAGCACCCGTCGCAAATCCAGGAGTTCTACCACGGGCCGTCGTCGCTGTCCACGGCTC 3300 AGGTCGTGGGCAGCGTTTAGGTCCTCAAGATGGTGCCCGGCAGCAGCGACAGGTGCCGAG 3301 CCTACCAGCAGAACCCGTGCGCCGTGGCGTGCCACGGGGACCCCGGCAATTTCTACGGCT 3360 GGATGGTCGTCTTGGGCACGCGGCACCGCACGGTGCCCCTGGGGCCGTTAAAGATGCCGA 3361 ACGACCCGCTGCAACGCCAGAGCCTATTCGGTGCGCAGGATCCAGACCTGGTGCAGTACG 3420 TGCTGGGCGACGTTGCGGTCTCGGATAAGCCACGCGTCCTAGGTCTGGACCACGTCATGC 3421 CAGACTGCAAGCTTGCCGCCGCCAGCGGCCTGGGCGAGGAGGCCGAGGGCTCCGAGCAGA 3480 GTCTGACGTTCGAACGGCGGCGGTCGCCGGACCCGCTCCTCCGGCTCCCGAGGCTCGTCT 3481 GCCCGTCGCCCACACAGCTCTTCCCCTGGATGCGCCCGCAAGCAGCCGCCGGACGCAGGC 3540 CGGGCAGCGGGTGTGTCGAGAAGGGGACCTACGCGGGCGTTCGTCGGCGGCCTGCGTCCG 3541 GAGGCCGACAGACCTACAGCCGCTACCAGACCCTGGAGCTGGAGAAGGAGTTCCTATTTA 3600 CTCCGGCTGTCTGGATGTCGGCGATGGTCTGGGACCTCGACCTCTTCCTCAAGGATAAAT 3601 ATCCCTATCTGACTCGTAAGCGGCGAATCGAGGTATCGCACGCCCTGGGACTGACAGAGA 3660 TAGGGATAGACTGAGCATTCGCCGCTTAGCTCCATAGCGTGCGGGACCCTGACTGTCTCT 3661 GACAGGTCAAAATCTGGTTCCAGAACCGGAGGATGAAGTGGAAAAAAGAGAACAACAAAG 3720 CTGTCCAGTTTTAGACCAAGGTCTTGGCCTCCTACTTCACCTTTTTTCTCTTGTTGTTTC 3721 ACAAGTTCCCCAGCAGCAAATGCGAGCAGGAGGAGCTGGAGAAACAGAAGCTGGAGCGGG 3780 TGTTCAAGGGGTCGTCGTTTACGCTCGTCCTCCTCGACCTCTTTGTCTTCGACCTCGCCC 3781 CCCCAGAGGCGGCGGACGAGGGCGACGCGCAGAAGGGCGACAAGAAGtgcccaactttct 3840 GGGGTCTCCGCCGCCTGCTCCCGCTGCGCGTCTTCCCGCTGTTCTTCACGGGTTGAAAGA XbaI SV40 prom(3876,4144)>>> | | 3841 tgtacaaagtggttgatatctgaggatcctctagaggtgtggaaagtccccaggctcccc 3900 ACATGTTTCACCAACTATAGACTCCTAGGAGATCTCCACACCTTTCAGGGGTCCGAGGGG 3901 agcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtc 3960 TCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAG 3961 cccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccat 4020 GGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTA SV40 origin(4043,4120)>>> | 4021 agtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctcc 4080 TCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGG SfiI | 4081 gccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctga 4140 CGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACT ORF_2 rf(1)(4192,5601)>>> | 4141 gctattccagaagtagtgaggaggcttttttggaggcctgtcgaggccaccatggagagc 4200 CGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGACAGCTCCGGTGGTACCTCTCG 4201 gacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggc 4260 CTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCG 4261 gtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaac 4320 CACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTG 4321 aagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatg 4380 TTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGGATGGACGACTCGGTGCACTAC 4381 ggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcac 4440 CCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCGATGCTCTTGGGGAAGGACGTG 4441 gccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtg 4500 CGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCAC 4501 ctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtg 4560 GACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCAC 4561 gtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaac 4620 CACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTG 4621 gccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgc 4680 CGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCACGACCACCCGTCGAAGCGGGCG 4681 accttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttc 4740 TGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCACCACCTGTCGGTGTACGTGAAG PstI | 4741 aagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgc 4800 TTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCG 4801 gtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaag 4860 CACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCACCTCATGGTCGTGCGGAAGTTC 4861 acccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcacc 4920 TGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGG 4921 gccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgac 4980 CGGCCTGGGCCGAGGTGGCCTAGAGCGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTG puro marker(5002,5601)>>> | 4981 gtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgc 5040 CACCTCCTCTTAGGGCCGGGATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCG 5041 gacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacg 5100 CTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGC 5101 cgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc 5160 GCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAG 5161 ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtg 5220 GAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCAC 5221 gcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccg 5280 CGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGC 5281 cgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctg 5340 GCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGAC 5341 gcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgac 5400 CGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTG 5401 caccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgc 5460 GTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCG 5461 gccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcgg 5520 CGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCC 5521 ctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg 5580 GAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTAC 5581 acccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgt 5640 TGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACA 5641 gaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgct 5700 CTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGA 5701 ttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtat 5760 AATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATA 5761 aaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtg 5820 TTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCAC 5821 gtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcag 5880 CACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTC 5881 ctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcc 5940 GAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGG 5941 tgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttg 6000 ACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAAC 6001 tcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgc 6060 AGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCG 6061 gggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggc 6120 CCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCG 6121 ctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatct 6180 GACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGA delta_U3 other(6211,6263)>>> | 6181 ccctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatac 6240 GGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATG HIV-1_5_LTR other(6264,6444)>>> | 6241 aagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagc 6300 TTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCG 6301 tctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttc 6360 AGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAG 6361 aagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccctttt 6420 TTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAA 6421 agtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttat 6480 TCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATA 6481 aacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatg 6540 TTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTAC 6541 gttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcatt 6600 CAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAA 6601 ctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcggg 6660 GATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCC 6661 tgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcg 6720 ACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGC 6721 gccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc 6780 CGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCG 6781 gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg 6840 CGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCC 6841 tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa 6900 ATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTT pBR322 origin(6950,7569)<<< | 6901 agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg 6960 TCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACC 6961 cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga 7020 GCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCT 7021 ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg 7080 CCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGC 7081 tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg 7140 ACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCC 7141 gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttc 7200 CTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAG 7201 gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg 7260 CGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGC 7261 gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca 7320 CATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGT 7321 ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggt 7380 GACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCA 7381 ggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccag 7440 CCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTC 7441 ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg 7500 AATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGC 7501 gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc 7560 CACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAG 7561 ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt 7620 GAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAA 7621 tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtt 7680 ACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAA 7681 ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca 7740 AATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGT 7741 gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg 7800 CACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGC 7801 tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatac 7860 AGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATG 7861 cgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg 7920 GCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCC 7921 ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc 7980 GGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGG 7981 gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggat 8040 CCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTA 8041 cttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggat 8100 GAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTA 8101 gaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggt 8160 CTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCA 8161 agcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcga 8220 TCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCT NEOKAN prom(8222,8271)>>> | 8221 accggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactg 8280 TGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGAC BclI | 8281 gatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagac 8340 CTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTG NTP_II marker(8363,9151)>>> | ORF_3 rf(2)(8360,9154)>>> | | 8341 aggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgc 8400 TCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCG 8401 ttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgc 8460 AACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACG 8461 cgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtc 8520 GCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAG 8521 cggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacggg 8580 GCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCC 8581 cgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctatt 8640 GCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAA 8641 gggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatc 8700 CCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAG 8701 catcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcga 8760 GTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCT 8761 ccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcga 8820 GGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCT 8821 tcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggct 8880 AGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGA 8881 caaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgcc 8940 GTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGG 8941 gaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgt 9000 CTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACA 9001 ggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcgg 9060 CCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCC 9061 cgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcat 9120 GCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTA 9121 cgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatca 9180 GCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGT 9181 gctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaataga 9240 CGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCT f1 origin(9259,9564)<<< | 9241 ccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg 9300 GGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACC 9301 actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccat 9360 TGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTA 9361 cacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaag 9420 GTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTC 9421 ggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggga 9480 CCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCT 9481 agaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaa 9540 TCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATT 9541 ccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 9600 GGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG 9601 gaattaattcttaattaacatcatcaataatatacctt 9638 CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9638 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3863 /sequence="ORF_4 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3870..3875 /dbxref="REBASE:XbaI" Promoter 3876..4144 /gene="SV40 prom" Rep_Origin 4043..4120 /gene="SV40 origin" misc_binding 4123..4135 /dbxref="REBASE:SfiI" ORF 4192..5601 /sequence="ORF_2 rf(1)" misc_binding 4765..4770 /dbxref="REBASE:PstI" Marker 5002..5601 /gene="puro marker" Other Gene 6211..6263 /gene="delta_U3 other" Other Gene 6264..6444 /gene="HIV-1_5_LTR other" Rep_Origin 6950..7569 /gene="pBR322 origin" Promoter 8222..8271 /gene="NEOKAN prom" misc_binding 8329..8334 /dbxref="REBASE:BclI" ORF 8360..9154 /sequence="ORF_3 rf(2)" Marker 8363..9151 /gene="NTP_II marker" Rep_Origin 9259..9564 /gene="f1 origin" BASE COUNT 2385 a 2498 c 2647 g 2108 t 0 others