pLenti-HOXA9-RFP
| Vector Name | pLenti-HOXA9-RFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg 3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg 3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc 3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga 3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc 3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca 3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc 3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg 3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa 3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca 3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga 3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca 3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga 3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc 3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatctga ctcgagtgaa 3961 tgaatgaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 4141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 4201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4261 tttggaggcc actagtgtcg agaaaactag tcgccaccat ggtgagcgag ctgattaagg 4321 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca 4381 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg 4441 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca 4501 aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccccgagg 4561 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg 4621 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc 4681 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagaccc 4741 tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg 4801 ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc 4861 tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca 4921 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca 4981 aactggggca cagagaattc ggaagcgggc agtgcaccaa ctacgccctg ctgaagctgg 5041 ccggcgacgt ggagagcaac cccggccccg gatccatggc caccgagtac aagcccacgg 5101 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 5161 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 5221 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 5281 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 5341 tcgccgagat cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 5401 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 5461 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 5521 tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 5581 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 5641 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc 5701 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5761 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5821 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 5881 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 5941 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 6001 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 6061 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 6121 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 6181 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc 6241 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat 6301 tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca 6361 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6421 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6481 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6541 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6721 ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg 6781 tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat gcgtaaggag 6841 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7441 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8101 cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa 8161 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc 8221 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt 8281 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag 8341 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca 8401 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 8461 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 8521 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 8581 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 8641 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 8701 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 8761 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 8821 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 8881 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 8941 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 9001 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 9061 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 9121 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 9181 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta 9241 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca acatccctta 9301 taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga acaagagtcc 9361 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 9421 cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct 9481 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 9541 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 9601 ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca gggcgcgtcc 9661 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat acctt //
LOCUS dna 9715 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3950 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" ORF 3072..4016 /sequence="ORF_5 rf(6)" misc_binding 3112..3117 /dbxref="REBASE:ApaI" misc_binding 3669..3674 /dbxref="REBASE:ClaI" misc_binding 3951..3956 /dbxref="REBASE:XhoI" Promoter 3968..4236 /gene="SV40 prom" Rep_Origin 4135..4212 /gene="SV40 origin" misc_binding 4215..4227 /dbxref="REBASE:SfiI" ORF 4299..5678 /sequence="ORF_4 rf(3)" misc_binding 4995..5000 /dbxref="REBASE:EcoRI" misc_binding 5070..5075 /dbxref="REBASE:BamHI" Marker 5082..5678 /gene="puro marker" misc_binding 5128..5133 /dbxref="REBASE:SmaI" misc_binding 5128..5133 /dbxref="REBASE:XmaI" Other Gene 6288..6340 /gene="delta_U3 other" Other Gene 6341..6521 /gene="HIV-1_5_LTR other" Rep_Origin 7027..7646 /gene="pBR322 origin" Promoter 8299..8348 /gene="NEOKAN prom" misc_binding 8406..8411 /dbxref="REBASE:BclI" ORF 8437..9231 /sequence="ORF_2 rf(1)" Marker 8440..9228 /gene="NTP_II marker" Rep_Origin 9336..9641 /gene="f1 origin" BASE COUNT 2438 a 2503 c 2638 g 2136 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg 3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg 3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc 3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga 3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc 3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca 3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc 3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg 3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa 3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca 3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga 3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca 3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga 3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc 3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatctga ctcgagtgaa 3961 tgaatgaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 4141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 4201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4261 tttggaggcc actagtgtcg agaaaactag tcgccaccat ggtgagcgag ctgattaagg 4321 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca 4381 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg 4441 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca 4501 aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccccgagg 4561 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg 4621 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc 4681 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagaccc 4741 tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg 4801 ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc 4861 tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca 4921 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca 4981 aactggggca cagagaattc ggaagcgggc agtgcaccaa ctacgccctg ctgaagctgg 5041 ccggcgacgt ggagagcaac cccggccccg gatccatggc caccgagtac aagcccacgg 5101 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 5161 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 5221 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 5281 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 5341 tcgccgagat cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 5401 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 5461 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 5521 tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 5581 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 5641 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc 5701 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5761 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5821 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 5881 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 5941 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 6001 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 6061 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 6121 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 6181 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc 6241 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat 6301 tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca 6361 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6421 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6481 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6541 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6721 ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg 6781 tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat gcgtaaggag 6841 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7441 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8101 cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa 8161 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc 8221 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt 8281 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag 8341 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca 8401 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 8461 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 8521 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 8581 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 8641 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 8701 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 8761 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 8821 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 8881 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 8941 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 9001 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 9061 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 9121 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 9181 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta 9241 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca acatccctta 9301 taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga acaagagtcc 9361 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 9421 cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct 9481 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 9541 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 9601 ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca gggcgcgtcc 9661 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat acctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_3 rf(3)(2901,3950)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC ORF_5 rf(6)(3072,4016)<<< ApaI | | 3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGGCCACCACTGGGGCCCTGG 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACCGGTGGTGACCCCGGGACC 3121 GCAACTACTACGTGGACTCGTTCCTGCTGGGCGCCGACGCCGCGGATGAGCTGAGCGTTG 3180 CGTTGATGATGCACCTGAGCAAGGACGACCCGCGGCTGCGGCGCCTACTCGACTCGCAAC 3181 GCCGCTATGCGCCGGGGACCCTGGGCCAGCCTCCCCGGCAGGCGGCGACGCTGGCCGAGC 3240 CGGCGATACGCGGCCCCTGGGACCCGGTCGGAGGGGCCGTCCGCCGCTGCGACCGGCTCG 3241 ACCCCGACTTCAGCCCGTGCAGCTTCCAGTCCAAGGCGACGGTGTTTGGCGCCTCGTGGA 3300 TGGGGCTGAAGTCGGGCACGTCGAAGGTCAGGTTCCGCTGCCACAAACCGCGGAGCACCT 3301 ACCCAGTGCACGCGGCGGGCGCCAACGCTGTACCCGCTGCGGTGTACCACCACCATCACC 3360 TGGGTCACGTGCGCCGCCCGCGGTTGCGACATGGGCGACGCCACATGGTGGTGGTAGTGG 3361 ACCACCCCTACGTGCACCCCCAGGCGCCCGTGGCGGCGGCGGCGCCGGACGGCAGGTACA 3420 TGGTGGGGATGCACGTGGGGGTCCGCGGGCACCGCCGCCGCCGCGGCCTGCCGTCCATGT 3421 TGCGCTCCTGGCTGGAGCCCACGCCCGGTGCGCTCTCCTTCGCGGGCTTGCCCTCCAGCC 3480 ACGCGAGGACCGACCTCGGGTGCGGGCCACGCGAGAGGAAGCGCCCGAACGGGAGGTCGG 3481 GGCCTTATGGCATTAAACCTGAACCGCTGTCGGCCAGAAGGGGTGACTGTCCCACGCTTG 3540 CCGGAATACCGTAATTTGGACTTGGCGACAGCCGGTCTTCCCCACTGACAGGGTGCGAAC 3541 ACACTCACACTTTGTCCCTGACTGACTATGCTTGTGGTTCTCCTCCAGTTGATAGAGAAA 3600 TGTGAGTGTGAAACAGGGACTGACTGATACGAACACCAAGAGGAGGTCAACTATCTCTTT 3601 AACAACCCAGCGAAGGCGCCTTCTCTGAAAACAATGCTGAGAATGAGAGCGGCGGAGACA 3660 TTGTTGGGTCGCTTCCGCGGAAGAGACTTTTGTTACGACTCTTACTCTCGCCGCCTCTGT ClaI | 3661 AGCCCCCCATCGATCCCAATAACCCAGCAGCCAACTGGCTTCATGCGCGCTCCACTCGGA 3720 TCGGGGGGTAGCTAGGGTTATTGGGTCGTCGGTTGACCGAAGTACGCGCGAGGTGAGCCT 3721 AAAAGCGGTGCCCCTATACAAAACACCAGACCCTGGAACTGGAGAAAGAGTTTCTGTTCA 3780 TTTTCGCCACGGGGATATGTTTTGTGGTCTGGGACCTTGACCTCTTTCTCAAAGACAAGT 3781 ACATGTACCTCACCAGGGACCGCAGGTACGAGGTGGCTCGACTGCTCAACCTCACCGAGA 3840 TGTACATGGAGTGGTCCCTGGCGTCCATGCTCCACCGAGCTGACGAGTTGGAGTGGCTCT 3841 GGCAGGTCAAGATCTGGTTCCAGAACCGCAGGATGAAAATGAAGAAAATCAACAAAGACC 3900 CCGTCCAGTTCTAGACCAAGGTCTTGGCGTCCTACTTTTACTTCTTTTAGTTGTTTCTGG XhoI | 3901 GAGCAAAAGACGAGtgcccaactttcttgtacaaagtggttgatatctgactcgagtgaa 3960 CTCGTTTTCTGCTCACGGGTTGAAAGAACATGTTTCACCAACTATAGACTGAGCTCACTT SV40 prom(3968,4236)>>> | 3961 tgaatgaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcat 4020 ACTTACTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTA 4021 ctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatg 4080 GAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATAC SV40 origin(4135,4212)>>> | 4081 caaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccg 4140 GTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGC 4141 cccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatt 4200 GGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAA SfiI | 4201 tatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttt 4260 ATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAA ORF_4 rf(3)(4299,5678)>>> | 4261 tttggaggccactagtgtcgagaaaactagtcgccaccatggtgagcgagctgattaagg 4320 AAACCTCCGGTGATCACAGCTCTTTTGATCAGCGGTGGTACCACTCGCTCGACTAATTCC 4321 agaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgca 4380 TCTTGTACGTGTACTTCGACATGTACCTCCCGTGGCACTTGTTGGTGGTGAAGTTCACGT 4381 catccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcg 4440 GTAGGCTCCCGCTTCCGTTCGGGATGCTCCCGTGGGTCTGGTACTCTTAGTTCCGCCAGC 4441 agggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagca 4500 TCCCGCCGGGAGAGGGGAAGCGGAAGCTGTAGGACCGATGGTCGAAGTACATGCCGTCGT 4501 aaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagg 4560 TTTGGAAGTAGTTGGTGTGGGTCCCGTAGGGGCTGAAGAAATTCGTCAGGAAGGGGCTCC 4561 gcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccagg 4620 CGAAGTGTACCCTCTCTCAGTGGTGTATGCTTCTGCCCCCGCACGACTGGCGATGGGTCC 4621 acaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcc 4680 TGTGGTCGGAGGTCCTGCCGACGGAGTAGATGTTGCAGTTCTAGTCTCCCCACTTGAAGG 4681 catccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccc 4740 GTAGGTTGCCGGGACACTACGTCTTCTTTTGTGAGCCGACCCTCCGGAGGTGGCTCTGGG 4741 tgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcg 4800 ACATGGGGCGACTGCCGCCGGACCTTCCGTCTCGGCTGTACCGGGACTTCGAGCACCCGC 4801 ggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacc 4860 CCCCGGTGGACTAGACGTTGAACTTCTGGTGTATGTCTAGGTTCTTTGGGCGATTCTTGG 4861 tcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgaca 4920 AGTTCTACGGGCCGCAGATGATACACCTGTCTTCTGACCTTTCTTAGTTCCTCCGGCTGT 4921 aagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagca 4980 TTCTCTGGATGCAGCTCGTCGTGCTCCACCGACACCGGTCTATGACGCTGGAGGGATCGT EcoRI | 4981 aactggggcacagagaattcggaagcgggcagtgcaccaactacgccctgctgaagctgg 5040 TTGACCCCGTGTCTCTTAAGCCTTCGCCCGTCACGTGGTTGATGCGGGACGACTTCGACC BamHI puro marker(5082,5678)>>> | | 5041 ccggcgacgtggagagcaaccccggccccggatccatggccaccgagtacaagcccacgg 5100 GGCCGCTGCACCTCTCGTTGGGGCCGGGGCCTAGGTACCGGTGGCTCATGTTCGGGTGCC SmaI | XmaI | | 5101 tgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcg 5160 ACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGC 5161 ccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccg 5220 GGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGC 5221 agctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcgg 5280 TCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCC 5281 acgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgt 5340 TGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACA 5341 tcgccgagatcggctcgcgcatggccgagttgagcggttcccggctggccgcgcagcaac 5400 AGCGGCTCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTG 5401 agatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccg 5460 TCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGC 5461 tcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggag 5520 AGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTC 5521 tggaggcggccgagcgcgctggggtgcccgccttcctggagacctccgcgccccgcaacc 5580 ACCTCCGCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGG 5581 tccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggac 5640 AGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTG 5641 cgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctc 5700 GCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAG 5701 tggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgc 5760 ACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCG 5761 tatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttca 5820 ATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGT 5821 ttttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttg 5880 AAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAAC 5881 tcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggca 5940 AGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGT 5941 ttgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacgg 6000 AACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCC 6001 cggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactg 6060 GCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGAC 6061 acaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttg 6120 TGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAAC 6121 ccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcgg 6180 GGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCC 6181 accttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccc 6240 TGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGG delta_U3 other(6288,6340)>>> | 6241 tcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaat 6300 AGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTA HIV-1_5_LTR other(6341,6521)>>> | 6301 tcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagacca 6360 AGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGT 6361 gatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaag 6420 CTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTC 6421 cttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagag 6480 GAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTC 6481 atccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatct 6540 TAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGA 6541 tattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtt 6600 ATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAA 6601 tattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagc 6660 ATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCG 6661 atttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgt 6720 TAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACA 6721 ctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatggg 6780 GACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCC 6781 tctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggag 6840 AGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTC 6841 aaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgt 6900 TTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCA 6901 tcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatc 6960 AGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAG 6961 aggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa 7020 TCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATT pBR322 origin(7027,7646)<<< | 7021 aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaa 7080 TTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTT 7081 tcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcc 7140 AGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGG 7141 ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtc 7200 GGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAG 7201 cgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcag 7260 GCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTC 7261 ttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccga 7320 AAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCT 7321 ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatc 7380 GGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAG 7381 gccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctac 7440 CGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATG 7441 agagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctg 7500 TCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGAC 7501 cgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaaca 7560 GCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGT 7561 aaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaa 7620 TTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTT 7621 aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaa 7680 TCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTT 7681 ctcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttt 7740 GAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAA 7741 aaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag 7800 TTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTC 7801 ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccat 7860 AATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTA 7861 agttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccc 7920 TCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGG 7921 cagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaa 7980 GTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTT 7981 ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcca 8040 GGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGT 8041 gtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaa 8100 CAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTT 8101 cgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaa 8160 GCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTT 8161 cggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagc 8220 GCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCG 8221 gcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggtt 8280 CGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAA NEOKAN prom(8299,8348)>>> | 8281 ttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaag 8340 AATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTC 8341 ccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatca 8400 GGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGT NTP_II marker(8440,9228)>>> | BclI ORF_2 rf(1)(8437,9231)>>> | | | 8401 agctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcac 8460 TCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTG 8461 gcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagaca 8520 CGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGT 8521 atcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttcttttt 8580 TAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAA 8581 gtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcg 8640 CAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGC 8641 tggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggga 8700 ACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCT 8701 agggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgct 8760 TCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGA 8761 cctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 8820 GGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGC 8821 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatg 8880 CGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTAC 8881 gaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagcc 8940 CTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGG 8941 gaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccat 9000 CTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTA 9001 ggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgac 9060 CCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTG 9061 tgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatatt 9120 ACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAA 9121 gctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgct 9180 CGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGA 9181 cccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtta 9240 GGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAAT 9241 aaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctta 9300 TTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAAT f1 origin(9336,9641)<<< | 9301 taaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcc 9360 ATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGG 9361 actattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatgg 9420 TGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACC 9421 cccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctct 9480 GGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGA 9481 aaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgt 9540 TTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCA 9541 ggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagc 9600 CCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCG 9601 ggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcc 9660 CCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGG 9661 attcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 9715 TAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9715 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..3950 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" ORF 3072..4016 /sequence="ORF_5 rf(6)" misc_binding 3112..3117 /dbxref="REBASE:ApaI" misc_binding 3669..3674 /dbxref="REBASE:ClaI" misc_binding 3951..3956 /dbxref="REBASE:XhoI" Promoter 3968..4236 /gene="SV40 prom" Rep_Origin 4135..4212 /gene="SV40 origin" misc_binding 4215..4227 /dbxref="REBASE:SfiI" ORF 4299..5678 /sequence="ORF_4 rf(3)" misc_binding 4995..5000 /dbxref="REBASE:EcoRI" misc_binding 5070..5075 /dbxref="REBASE:BamHI" Marker 5082..5678 /gene="puro marker" misc_binding 5128..5133 /dbxref="REBASE:SmaI" misc_binding 5128..5133 /dbxref="REBASE:XmaI" Other Gene 6288..6340 /gene="delta_U3 other" Other Gene 6341..6521 /gene="HIV-1_5_LTR other" Rep_Origin 7027..7646 /gene="pBR322 origin" Promoter 8299..8348 /gene="NEOKAN prom" misc_binding 8406..8411 /dbxref="REBASE:BclI" ORF 8437..9231 /sequence="ORF_2 rf(1)" Marker 8440..9228 /gene="NTP_II marker" Rep_Origin 9336..9641 /gene="f1 origin" BASE COUNT 2438 a 2503 c 2638 g 2136 t 0 others