pLenti-HOXA10-RFP
| Vector Name | pLenti-HOXA10-RFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg 4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca 4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg 4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg 4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct 4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca 4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca 5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca 5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc 5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc 5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga 5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga 5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg 5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg 5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc 5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt 6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac 6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc 6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca 7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 10129 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4364 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3321..3326 /dbxref="REBASE:PstI" Promoter 4382..4650 /gene="SV40 prom" Rep_Origin 4549..4626 /gene="SV40 origin" ORF 4713..6092 /sequence="ORF_4 rf(3)" misc_binding 5409..5414 /dbxref="REBASE:EcoRI" Marker 5496..6092 /gene="puro marker" Other Gene 6702..6754 /gene="delta_U3 other" Other Gene 6755..6935 /gene="HIV-1_5_LTR other" Rep_Origin 7441..8060 /gene="pBR322 origin" Promoter 8713..8762 /gene="NEOKAN prom" misc_binding 8820..8825 /dbxref="REBASE:BclI" ORF 8851..9645 /sequence="ORF_2 rf(1)" Marker 8854..9642 /gene="NTP_II marker" Rep_Origin 9750..10055 /gene="f1 origin" BASE COUNT 2477 a 2665 c 2804 g 2183 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg 4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca 4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg 4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg 4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct 4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca 4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca 5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca 5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc 5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc 5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga 5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga 5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg 5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg 5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc 5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt 6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac 6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc 6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca 7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_3 rf(3)(2901,4364)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGTCAGCCAGAAAGGGCTATC 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACAGTCGGTCTTTCCCGATAG 3121 TGCTCCCTTCGCCAAATTATCCCACAACAATGTCATGCTCGGAGAGCCCCGCCGCGAACT 3180 ACGAGGGAAGCGGTTTAATAGGGTGTTGTTACAGTACGAGCCTCTCGGGGCGGCGCTTGA 3181 CTTTTTTGGTCGACTCGCTCATCAGCTCGGGCAGAGGCGAGGCAGGCGGCGGTGGTGGTG 3240 GAAAAAACCAGCTGAGCGAGTAGTCGAGCCCGTCTCCGCTCCGTCCGCCGCCACCACCAC 3241 GCGCGGGGGGCGGCGGCGGTGGCGGTTACTACGCCCACGGCGGGGTCTACCTGCCGCCCG 3300 CGCGCCCCCCGCCGCCGCCACCGCCAATGATGCGGGTGCCGCCCCAGATGGACGGCGGGC PstI | 3301 CCGCCGACCTGCCCTACGGGCTGCAGAGCTGCGGGCTCTTCCCCACGCTGGGCGGCAAGC 3360 GGCGGCTGGACGGGATGCCCGACGTCTCGACGCCCGAGAAGGGGTGCGACCCGCCGTTCG 3361 GCAATGAGGCAGCGTCGCCGGGCAGCGGTGGCGGTGGCGGGGGTCTAGGTCCCGGGGCGC 3420 CGTTACTCCGTCGCAGCGGCCCGTCGCCACCGCCACCGCCCCCAGATCCAGGGCCCCGCG 3421 ACGGCTACGGGCCCTCGCCCATAGACCTGTGGCTAGACGCGCCCCGGTCTTGCCGGATGG 3480 TGCCGATGCCCGGGAGCGGGTATCTGGACACCGATCTGCGCGGGGCCAGAACGGCCTACC 3481 AGCCGCCTGACGGGCCGCCGCCGCCGCCCCAGCAGCAGCCGCCGCCCCCGCCGCAACCAC 3540 TCGGCGGACTGCCCGGCGGCGGCGGCGGGGTCGTCGTCGGCGGCGGGGGCGGCGTTGGTG 3541 CCCAGCCAGCGCCGCAGGCCACCTCGTGCTCTTTCGCGCAGAACATCAAAGAAGAGAGCT 3600 GGGTCGGTCGCGGCGTCCGGTGGAGCACGAGAAAGCGCGTCTTGTAGTTTCTTCTCTCGA 3601 CCTACTGCCTCTACGACTCGGCGGACAAATGCCCCAAAGTCTCGGCCACCGCCGCCGAAC 3660 GGATGACGGAGATGCTGAGCCGCCTGTTTACGGGGTTTCAGAGCCGGTGGCGGCGGCTTG 3661 TGGCTCCCTTCCCGCGGGGCCCGCCGCCCGACGGCTGCGCCCTGGGCACCTCCAGCGGGG 3720 ACCGAGGGAAGGGCGCCCCGGGCGGCGGGCTGCCGACGCGGGACCCGTGGAGGTCGCCCC 3721 TGCCAGTGCCTGGCTACTTCCGCCTTTCTCAGGCCTACGGCACCGCCAAGGGCTATGGCA 3780 ACGGTCACGGACCGATGAAGGCGGAAAGAGTCCGGATGCCGTGGCGGTTCCCGATACCGT 3781 GCGGCGGCGGCGGCGCGCAGCAACTCGGGGCTGGCCCGTTCCCCGCGCAGCCCCCGGGGC 3840 CGCCGCCGCCGCCGCGCGTCGTTGAGCCCCGACCGGGCAAGGGGCGCGTCGGGGGCCCCG 3841 GCGGTTTCGATCTCCCGCCCGCGCTAGCCTCCGGCTCGGCCGATGCGGCCCGGAAGGAGC 3900 CGCCAAAGCTAGAGGGCGGGCGCGATCGGAGGCCGAGCCGGCTACGCCGGGCCTTCCTCG 3901 GAGCCCTCGATTCGCCGCCGCCCCCCACGCTGGCTTGCGGCAGCGGCGGGGGCTCGCAGG 3960 CTCGGGAGCTAAGCGGCGGCGGGGGGTGCGACCGAACGCCGTCGCCGCCCCCGAGCGTCC 3961 GCGACGAGGAGGCGCACGCGTCGTCCTCGGCCGCGGAGGAGCTCTCCCCGGCCCCTTCCG 4020 CGCTGCTCCTCCGCGTGCGCAGCAGGAGCCGGCGCCTCCTCGAGAGGGGCCGGGGAAGGC 4021 AGAGCAGCAAAGCCTCGCCGGAGAAGGATTCCCTGGGCAATTCCAAAGGTGAAAACGCAG 4080 TCTCGTCGTTTCGGAGCGGCCTCTTCCTAAGGGACCCGTTAAGGTTTCCACTTTTGCGTC 4081 CCAACTGGCTCACGGCAAAGAGTGGTCGGAAGAAGCGCTGCCCCTACACGAAGCACCAGA 4140 GGTTGACCGAGTGCCGTTTCTCACCAGCCTTCTTCGCGACGGGGATGTGCTTCGTGGTCT 4141 CACTGGAGCTGGAGAAGGAGTTTCTGTTCAATATGTACCTTACTCGAGAGCGGCGCCTAG 4200 GTGACCTCGACCTCTTCCTCAAAGACAAGTTATACATGGAATGAGCTCTCGCCGCGGATC 4201 AGATTAGCCGCAGCGTCCACCTCACGGACAGACAAGTGAAAATCTGGTTTCAGAACCGCA 4260 TCTAATCGGCGTCGCAGGTGGAGTGCCTGTCTGTTCACTTTTAGACCAAAGTCTTGGCGT 4261 GGATGAAACTGAAGAAAATGAATCGAGAAAACCGGATCCGGGAGCTCACAGCCAACTTTA 4320 CCTACTTTGACTTCTTTTACTTAGCTCTTTTGGCCTAGGCCCTCGAGTGTCGGTTGAAAT 4321 ATTTTTCCtgcccaactttcttgtacaaagtggttgatatctgactcgagtgaatgaatg 4380 TAAAAAGGACGGGTTGAAAGAACATGTTTCACCAACTATAGACTGAGCTCACTTACTTAC SV40 prom(4382,4650)>>> | 4381 aggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaat 4440 TCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTA 4441 tagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagc 4500 ATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCG SV40 origin(4549,4626)>>> | 4501 atgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgccccta 4560 TACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGAT 4561 actccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgca 4620 TGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGT 4621 gaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttgga 4680 CTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCT ORF_4 rf(3)(4713,6092)>>> | 4681 ggccactagtgtcgagaaaactagtcgccaccatggtgagcgagctgattaaggagaaca 4740 CCGGTGATCACAGCTCTTTTGATCAGCGGTGGTACCACTCGCTCGACTAATTCCTCTTGT 4741 tgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccg 4800 ACGTGTACTTCGACATGTACCTCCCGTGGCACTTGTTGGTGGTGAAGTTCACGTGTAGGC 4801 agggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcg 4860 TCCCGCTTCCGTTCGGGATGCTCCCGTGGGTCTGGTACTCTTAGTTCCGCCAGCTCCCGC 4861 gccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaacct 4920 CGGGAGAGGGGAAGCGGAAGCTGTAGGACCGATGGTCGAAGTACATGCCGTCGTTTTGGA 4921 tcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttca 4980 AGTAGTTGGTGTGGGTCCCGTAGGGGCTGAAGAAATTCGTCAGGAAGGGGCTCCCGAAGT 4981 catgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacacca 5040 GTACCCTCTCTCAGTGGTGTATGCTTCTGCCCCCGCACGACTGGCGATGGGTCCTGTGGT 5041 gcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatcca 5100 CGGAGGTCCTGCCGACGGAGTAGATGTTGCAGTTCTAGTCTCCCCACTTGAAGGGTAGGT 5101 acggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtacc 5160 TGCCGGGACACTACGTCTTCTTTTGTGAGCCGACCCTCCGGAGGTGGCTCTGGGACATGG 5161 ccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggcc 5220 GGCGACTGCCGCCGGACCTTCCGTCTCGGCTGTACCGGGACTTCGAGCACCCGCCCCCGG 5221 acctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaaga 5280 TGGACTAGACGTTGAACTTCTGGTGTATGTCTAGGTTCTTTGGGCGATTCTTGGAGTTCT 5281 tgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagaga 5340 ACGGGCCGCAGATGATACACCTGTCTTCTGACCTTTCTTAGTTCCTCCGGCTGTTTCTCT 5341 cctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactgg 5400 GGATGCAGCTCGTCGTGCTCCACCGACACCGGTCTATGACGCTGGAGGGATCGTTTGACC EcoRI | 5401 ggcacagagaattcggaagcgggcagtgcaccaactacgccctgctgaagctggccggcg 5460 CCGTGTCTCTTAAGCCTTCGCCCGTCACGTGGTTGATGCGGGACGACTTCGACCGGCCGC puro marker(5496,6092)>>> | 5461 acgtggagagcaaccccggccccggatccatggccaccgagtacaagcccacggtgcgcc 5520 TGCACCTCTCGTTGGGGCCGGGGCCTAGGTACCGGTGGCTCATGTTCGGGTGCCACGCGG 5521 tcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgact 5580 AGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGA 5581 accccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgc 5640 TGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACG 5641 aagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacg 5700 TTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGC 5701 gcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccg 5760 CGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGC 5761 agatcggctcgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatgg 5820 TCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACC 5821 aaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcg 5880 TTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGC 5881 tctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggagg 5940 AGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCC 5941 cggccgagcgcgctggggtgcccgccttcctggagacctccgcgccccgcaacctcccct 6000 GCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGA 6001 tctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgca 6060 AGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGT 6061 cctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggatt 6120 GGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAA 6121 acaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtg 6180 TGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACAC 6181 gatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttct 6240 CTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGA 6241 cctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggc 6300 GGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCG 6301 aacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcca 6360 TTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGT 6361 ccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaac 6420 GGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTG 6421 tcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaatt 6480 AGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAA 6481 ccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacct 6540 GGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGA 6541 ggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttc 6600 CCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAG 6601 cttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagac 6660 GAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTG delta_U3 other(6702,6754)>>> | 6661 gagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactc 6720 CTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAG HIV-1_5_LTR other(6755,6935)>>> | 6721 ccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctg 6780 GGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGAC 6781 agcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcc 6840 TCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGG 6841 ttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct 6900 AACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGA 6901 cagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattat 6960 GTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATA 6961 tcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgc 7020 AGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACG 7021 agcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttt 7080 TCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAA 7081 ttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggca 7140 AAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGT 7141 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 7200 AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT 7201 ttaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaata 7260 AATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTAT 7261 ccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct 7320 GGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA 7321 gcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggga 7380 CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT 7381 taacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc 7440 ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG pBR322 origin(7441,8060)<<< | 7441 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg 7500 GCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGC 7501 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg 7560 GAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACC 7561 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt 7620 TTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAA 7621 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt 7680 AGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCA 7681 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg 7740 CATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGAC 7741 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact 7800 GCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGA 7801 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt 7860 CCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAA 7861 cttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctct 7920 GAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGA 7921 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac 7980 CGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTG 7981 cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc 8040 GCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAG 8041 tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacg 8100 AGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGC 8101 ttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta 8160 AATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAT 8161 aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacca 8220 TTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGT 8221 atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgc 8280 TACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACG 8281 ctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc 8340 GACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACG 8341 tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc 8400 ACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGG 8401 agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctat 8460 TCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATA 8461 taattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgt 8520 ATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACA 8521 tgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgc 8580 ACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACG 8581 tgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaag 8640 ACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTC 8641 agaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatgg 8700 TCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACC NEOKAN prom(8713,8762)>>> | 8701 acagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgc 8760 TGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACG 8761 aaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctct 8820 TTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGA NTP_II marker(8854,9642)>>> | BclI ORF_2 rf(1)(8851,9645)>>> | | | 8821 gatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggt 8880 CTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCA 8881 tctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggc 8940 AGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCG 8941 tgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag 9000 ACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTC 9001 accgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctg 9060 TGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGAC 9061 gccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggac 9120 CGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTG 9121 tggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgcc 9180 ACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGG 9181 gagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacc 9240 CTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGG 9241 tgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagcc 9300 ACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGG 9301 ggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactg 9360 CCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGAC 9361 ttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgat 9420 AAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTA 9421 gcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggc 9480 CGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCG 9481 cggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaa 9540 GCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTT 9541 gagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgat 9600 CTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTA 9601 tcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaattt 9660 AGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAA 9661 ttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatc 9720 AACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAG f1 origin(9750,10055)<<< | 9721 aaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactatt 9780 TTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAA 9781 aaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccact 9840 TTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGA 9841 acgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcg 9900 TGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGC 9901 gaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgag 9960 CTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTC 9961 aaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcac 10020 TTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTG 10021 gctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgc 10080 CGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCG 10081 cattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10129 GTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10129 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4364 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3321..3326 /dbxref="REBASE:PstI" Promoter 4382..4650 /gene="SV40 prom" Rep_Origin 4549..4626 /gene="SV40 origin" ORF 4713..6092 /sequence="ORF_4 rf(3)" misc_binding 5409..5414 /dbxref="REBASE:EcoRI" Marker 5496..6092 /gene="puro marker" Other Gene 6702..6754 /gene="delta_U3 other" Other Gene 6755..6935 /gene="HIV-1_5_LTR other" Rep_Origin 7441..8060 /gene="pBR322 origin" Promoter 8713..8762 /gene="NEOKAN prom" misc_binding 8820..8825 /dbxref="REBASE:BclI" ORF 8851..9645 /sequence="ORF_2 rf(1)" Marker 8854..9642 /gene="NTP_II marker" Rep_Origin 9750..10055 /gene="f1 origin" BASE COUNT 2477 a 2665 c 2804 g 2183 t 0 others