pLenti-HOXA10-GFP
| Vector Name | pLenti-HOXA10-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgaggatcc tctagaggtg 4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 4681 gtcgaggcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 4741 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 4801 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 4861 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 4921 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 4981 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5041 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5101 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5161 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5221 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5281 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5341 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5401 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5461 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5521 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5581 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5641 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5701 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5761 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5821 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5881 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5941 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6001 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6061 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa 6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc 6661 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc 6721 taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag 6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 6961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 7021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7141 atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta 7201 tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa 7261 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 7921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8521 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca 8581 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc 8641 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 8701 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg 8761 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg 8821 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 8881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 8941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 9001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 9061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 9121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 9181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 9241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 9301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 9361 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 9421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 9481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 9541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 9601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt 9661 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc 9721 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga 9781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 9841 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag 9901 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 9961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 10021 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc 10081 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 10139 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4364 /sequence="ORF_4 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 4183..4188 /dbxref="REBASE:XhoI" misc_binding 4371..4376 /dbxref="REBASE:XbaI" Promoter 4377..4645 /gene="SV40 prom" Rep_Origin 4544..4621 /gene="SV40 origin" ORF 4693..6102 /sequence="ORF_2 rf(1)" Marker 5503..6102 /gene="puro marker" Other Gene 6712..6764 /gene="delta_U3 other" Other Gene 6765..6945 /gene="HIV-1_5_LTR other" Rep_Origin 7451..8070 /gene="pBR322 origin" Promoter 8723..8772 /gene="NEOKAN prom" misc_binding 8830..8835 /dbxref="REBASE:BclI" ORF 8861..9655 /sequence="ORF_3 rf(2)" Marker 8864..9652 /gene="NTP_II marker" Rep_Origin 9760..10065 /gene="f1 origin" BASE COUNT 2431 a 2703 c 2822 g 2183 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgaggatcc tctagaggtg 4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 4681 gtcgaggcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 4741 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 4801 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 4861 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 4921 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 4981 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5041 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5101 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5161 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5221 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5281 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5341 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5401 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5461 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5521 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5581 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5641 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5701 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5761 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5821 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5881 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5941 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6001 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6061 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa 6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc 6661 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc 6721 taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag 6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 6961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 7021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7141 atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta 7201 tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa 7261 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 7921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8521 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca 8581 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc 8641 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 8701 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg 8761 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg 8821 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 8881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 8941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 9001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 9061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 9121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 9181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 9241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 9301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 9361 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 9421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 9481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 9541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 9601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt 9661 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc 9721 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga 9781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 9841 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag 9901 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 9961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 10021 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc 10081 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_4 rf(3)(2901,4364)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGTCAGCCAGAAAGGGCTATC 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACAGTCGGTCTTTCCCGATAG 3121 TGCTCCCTTCGCCAAATTATCCCACAACAATGTCATGCTCGGAGAGCCCCGCCGCGAACT 3180 ACGAGGGAAGCGGTTTAATAGGGTGTTGTTACAGTACGAGCCTCTCGGGGCGGCGCTTGA 3181 CTTTTTTGGTCGACTCGCTCATCAGCTCGGGCAGAGGCGAGGCAGGCGGCGGTGGTGGTG 3240 GAAAAAACCAGCTGAGCGAGTAGTCGAGCCCGTCTCCGCTCCGTCCGCCGCCACCACCAC 3241 GCGCGGGGGGCGGCGGCGGTGGCGGTTACTACGCCCACGGCGGGGTCTACCTGCCGCCCG 3300 CGCGCCCCCCGCCGCCGCCACCGCCAATGATGCGGGTGCCGCCCCAGATGGACGGCGGGC 3301 CCGCCGACCTGCCCTACGGGCTGCAGAGCTGCGGGCTCTTCCCCACGCTGGGCGGCAAGC 3360 GGCGGCTGGACGGGATGCCCGACGTCTCGACGCCCGAGAAGGGGTGCGACCCGCCGTTCG 3361 GCAATGAGGCAGCGTCGCCGGGCAGCGGTGGCGGTGGCGGGGGTCTAGGTCCCGGGGCGC 3420 CGTTACTCCGTCGCAGCGGCCCGTCGCCACCGCCACCGCCCCCAGATCCAGGGCCCCGCG 3421 ACGGCTACGGGCCCTCGCCCATAGACCTGTGGCTAGACGCGCCCCGGTCTTGCCGGATGG 3480 TGCCGATGCCCGGGAGCGGGTATCTGGACACCGATCTGCGCGGGGCCAGAACGGCCTACC 3481 AGCCGCCTGACGGGCCGCCGCCGCCGCCCCAGCAGCAGCCGCCGCCCCCGCCGCAACCAC 3540 TCGGCGGACTGCCCGGCGGCGGCGGCGGGGTCGTCGTCGGCGGCGGGGGCGGCGTTGGTG 3541 CCCAGCCAGCGCCGCAGGCCACCTCGTGCTCTTTCGCGCAGAACATCAAAGAAGAGAGCT 3600 GGGTCGGTCGCGGCGTCCGGTGGAGCACGAGAAAGCGCGTCTTGTAGTTTCTTCTCTCGA 3601 CCTACTGCCTCTACGACTCGGCGGACAAATGCCCCAAAGTCTCGGCCACCGCCGCCGAAC 3660 GGATGACGGAGATGCTGAGCCGCCTGTTTACGGGGTTTCAGAGCCGGTGGCGGCGGCTTG 3661 TGGCTCCCTTCCCGCGGGGCCCGCCGCCCGACGGCTGCGCCCTGGGCACCTCCAGCGGGG 3720 ACCGAGGGAAGGGCGCCCCGGGCGGCGGGCTGCCGACGCGGGACCCGTGGAGGTCGCCCC 3721 TGCCAGTGCCTGGCTACTTCCGCCTTTCTCAGGCCTACGGCACCGCCAAGGGCTATGGCA 3780 ACGGTCACGGACCGATGAAGGCGGAAAGAGTCCGGATGCCGTGGCGGTTCCCGATACCGT 3781 GCGGCGGCGGCGGCGCGCAGCAACTCGGGGCTGGCCCGTTCCCCGCGCAGCCCCCGGGGC 3840 CGCCGCCGCCGCCGCGCGTCGTTGAGCCCCGACCGGGCAAGGGGCGCGTCGGGGGCCCCG 3841 GCGGTTTCGATCTCCCGCCCGCGCTAGCCTCCGGCTCGGCCGATGCGGCCCGGAAGGAGC 3900 CGCCAAAGCTAGAGGGCGGGCGCGATCGGAGGCCGAGCCGGCTACGCCGGGCCTTCCTCG 3901 GAGCCCTCGATTCGCCGCCGCCCCCCACGCTGGCTTGCGGCAGCGGCGGGGGCTCGCAGG 3960 CTCGGGAGCTAAGCGGCGGCGGGGGGTGCGACCGAACGCCGTCGCCGCCCCCGAGCGTCC 3961 GCGACGAGGAGGCGCACGCGTCGTCCTCGGCCGCGGAGGAGCTCTCCCCGGCCCCTTCCG 4020 CGCTGCTCCTCCGCGTGCGCAGCAGGAGCCGGCGCCTCCTCGAGAGGGGCCGGGGAAGGC 4021 AGAGCAGCAAAGCCTCGCCGGAGAAGGATTCCCTGGGCAATTCCAAAGGTGAAAACGCAG 4080 TCTCGTCGTTTCGGAGCGGCCTCTTCCTAAGGGACCCGTTAAGGTTTCCACTTTTGCGTC 4081 CCAACTGGCTCACGGCAAAGAGTGGTCGGAAGAAGCGCTGCCCCTACACGAAGCACCAGA 4140 GGTTGACCGAGTGCCGTTTCTCACCAGCCTTCTTCGCGACGGGGATGTGCTTCGTGGTCT XhoI | 4141 CACTGGAGCTGGAGAAGGAGTTTCTGTTCAATATGTACCTTACTCGAGAGCGGCGCCTAG 4200 GTGACCTCGACCTCTTCCTCAAAGACAAGTTATACATGGAATGAGCTCTCGCCGCGGATC 4201 AGATTAGCCGCAGCGTCCACCTCACGGACAGACAAGTGAAAATCTGGTTTCAGAACCGCA 4260 TCTAATCGGCGTCGCAGGTGGAGTGCCTGTCTGTTCACTTTTAGACCAAAGTCTTGGCGT 4261 GGATGAAACTGAAGAAAATGAATCGAGAAAACCGGATCCGGGAGCTCACAGCCAACTTTA 4320 CCTACTTTGACTTCTTTTACTTAGCTCTTTTGGCCTAGGCCCTCGAGTGTCGGTTGAAAT XbaI SV40 prom(4377,4645)>>> | | 4321 ATTTTTCCtgcccaactttcttgtacaaagtggttgatatctgaggatcctctagaggtg 4380 TAAAAAGGACGGGTTGAAAGAACATGTTTCACCAACTATAGACTCCTAGGAGATCTCCAC 4381 tggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc 4440 ACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAG 4441 agcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgca 4500 TCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGT SV40 origin(4544,4621)>>> | 4501 tctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactcc 4560 AGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGG 4561 gcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggc 4620 CGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCG 4621 cgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcct 4680 GCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGA ORF_2 rf(1)(4693,6102)>>> | 4681 gtcgaggccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgc 4740 CAGCTCCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCG 4741 atcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcaccccc 4800 TAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGG 4801 aagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccc 4860 TTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGG 4861 tacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggc 4920 ATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCG 4921 tacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgag 4980 ATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTC 4981 aagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgc 5040 TTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCG 5041 gtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcacc 5100 CACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGG 5101 gacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtg 5160 CTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCAC 5161 ctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtg 5220 GACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCAC 5221 gtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggc 5280 CACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCG 5281 cccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtg 5340 GGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCAC 5341 gagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtcc 5400 CTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGG 5401 aattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagagga 5460 TTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGCGCTCCCGTCTCCT puro marker(5503,6102)>>> | 5461 agtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagccc 5520 TCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGATACTGGCTCATGTTCGGG 5521 acggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcg 5580 TGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGC 5581 ttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtc 5640 AAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAG 5641 accgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtc 5700 TGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAG 5701 gcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcg 5760 CGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGC 5761 gtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcag 5820 CACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTC 5821 caacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggcc 5880 GTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGG 5881 accgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctcccc 5940 TGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGG 5941 ggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgc 6000 CCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCG 6001 aacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaa 6060 TTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTT 6061 ggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaa 6120 CCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTT 6121 cctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctcctttt 6180 GGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAA 6181 acgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggct 6240 TGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGA 6241 ttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggccc 6300 AAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGG 6301 gttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttgg 6360 CAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACC 6361 ggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgcc 6420 CCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGG 6421 acggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggc 6480 TGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCG 6481 actgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgt 6540 TGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACA 6541 gttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatcca 6600 CAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGT 6601 gcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttc 6660 CGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAG delta_U3 other(6712,6764)>>> | 6661 gccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggc 6720 CGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCG HIV-1_5_LTR other(6765,6945)>>> | 6721 taattcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttag 6780 ATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATC 6781 accagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat 6840 TGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTA 6841 aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaact 6900 TTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGA 6901 agagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtc 6960 TCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAG 6961 atcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaact 7020 TAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGA 7021 tgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaata 7080 ACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTAT 7081 aagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatc 7140 TTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAG 7141 atgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtatta 7200 TACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAAT 7201 tgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaa 7260 ACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATT 7261 ggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcgg 7320 CCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCC 7321 tcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacag 7380 AGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTC 7381 aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc 7440 TTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGG pBR322 origin(7451,8070)<<< | 7441 gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcaca 7500 CATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGT 7501 aaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgt 7560 TTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCA 7561 ttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacc 7620 AAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGG 7621 tgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc 7680 ACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAG 7681 tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagc 7740 AGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCG 7741 ccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact 7800 GGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGA 7801 tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtg 7860 ATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCAC 7861 ctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta 7920 GATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCAT 7921 tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca 7980 AGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGT 7981 aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa 8040 TTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTT 8041 aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg 8100 TTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGC 8101 aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc 8160 TTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGG 8161 ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctg 8220 AAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGAC 8221 acagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcat 8280 TGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTA 8281 ccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctg 8340 GGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGAC 8341 gccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa 8400 CGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTT 8401 taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcca 8460 ATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGT 8461 tccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgc 8520 AGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACG 8521 gcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgca 8580 CGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGT 8581 gaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgc 8640 CTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCG 8641 aagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggc 8700 TTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCG NEOKAN prom(8723,8772)>>> | 8701 ggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgg 8760 CCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACC 8761 gaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcagggg 8820 CTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCC NTP_II marker(8864,9652)>>> | BclI ORF_3 rf(2)(8861,9655)>>> | | | 8821 atcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggatt 8880 TAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAA 8881 gcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaaca 8940 CGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGT 8941 gacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttct 9000 CTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGA 9001 ttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggct 9060 AAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGA 9061 atcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagc 9120 TAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCG 9121 gggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 9180 CCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGA 9181 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttga 9240 ACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACT 9241 tccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcg 9300 AGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGC 9301 gatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcc 9360 CTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGG 9361 agccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgac 9420 TCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTG 9421 ccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcat 9480 GGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTA 9481 cgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtga 9540 GCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACT 9541 tattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgc 9600 ATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCG 9601 cgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaatttt 9660 GCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAA 9661 gttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcc 9720 CAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGG f1 origin(9760,10065)<<< | 9721 cttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaaga 9780 GAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCT 9781 gtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcg 9840 CAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGC 9841 atggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaag 9900 TACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTC 9901 ctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcga 9960 GAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCT 9961 acgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtg 10020 TGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCAC 10021 tagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgc 10080 ATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCG 10081 gtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10139 CAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10139 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4364 /sequence="ORF_4 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 4183..4188 /dbxref="REBASE:XhoI" misc_binding 4371..4376 /dbxref="REBASE:XbaI" Promoter 4377..4645 /gene="SV40 prom" Rep_Origin 4544..4621 /gene="SV40 origin" ORF 4693..6102 /sequence="ORF_2 rf(1)" Marker 5503..6102 /gene="puro marker" Other Gene 6712..6764 /gene="delta_U3 other" Other Gene 6765..6945 /gene="HIV-1_5_LTR other" Rep_Origin 7451..8070 /gene="pBR322 origin" Promoter 8723..8772 /gene="NEOKAN prom" misc_binding 8830..8835 /dbxref="REBASE:BclI" ORF 8861..9655 /sequence="ORF_3 rf(2)" Marker 8864..9652 /gene="NTP_II marker" Rep_Origin 9760..10065 /gene="f1 origin" BASE COUNT 2431 a 2703 c 2822 g 2183 t 0 others