pLenti-HOXA10-C-term-HA
Vector Name | pLenti-HOXA10-C-term-HA |
VectorType | Lentiviral Vector |
Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat cctcgagtac ccatacgacg 4381 tcccagacta cgcttgagtt taaacacgcg tggtgtggaa agtccccagg ctccccagca 4441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4681 ttccagaagt agtgaggagg cttttttgga ggccatgacc gagtacaagc ccacggtgcg 4741 cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc ctcgccgccg cgttcgccga 4801 ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac atcgagcggg tcaccgagct 4861 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 4921 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 4981 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5041 ggaagggctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5101 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 5161 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 5221 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 5281 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt tccggaaatc aacctctgga 5341 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5401 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5461 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5521 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5581 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5641 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5701 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5761 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5821 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag 5881 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac 5941 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 6181 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 6241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 6301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 6361 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg 6421 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa 6481 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 7021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 7081 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 7141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7741 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 7801 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 7861 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 7921 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 7981 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct 8041 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 8101 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 8161 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 8221 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 8281 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 8341 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 8401 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 8461 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 8521 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 8581 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 8641 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 8701 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 8761 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 8821 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat 8881 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa 8941 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta 9001 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 9061 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat 9121 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 9181 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 9241 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc 9301 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t
LOCUS dna 9351 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4397 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3321..3326 /dbxref="REBASE:PstI" misc_binding 3751..3756 /dbxref="REBASE:StuI" misc_binding 4294..4299 /dbxref="REBASE:BamHI" Promoter 4412..4680 /gene="SV40 prom" Rep_Origin 4579..4656 /gene="SV40 origin" ORF 4646..5314 /sequence="ORF_2 rf(2)" Marker 4715..5314 /gene="puro marker" Other Gene 5924..5976 /gene="delta_U3 other" Other Gene 5977..6157 /gene="HIV-1_5_LTR other" Rep_Origin 6663..7282 /gene="pBR322 origin" Promoter 7935..7984 /gene="NEOKAN prom" misc_binding 8042..8047 /dbxref="REBASE:BclI" ORF 8073..8867 /sequence="ORF_4 rf(3)" Marker 8076..8864 /gene="NTP_II marker" Rep_Origin 8972..9277 /gene="f1 origin" BASE COUNT 2271 a 2426 c 2584 g 2070 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat cctcgagtac ccatacgacg 4381 tcccagacta cgcttgagtt taaacacgcg tggtgtggaa agtccccagg ctccccagca 4441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4681 ttccagaagt agtgaggagg cttttttgga ggccatgacc gagtacaagc ccacggtgcg 4741 cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc ctcgccgccg cgttcgccga 4801 ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac atcgagcggg tcaccgagct 4861 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 4921 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 4981 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5041 ggaagggctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5101 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 5161 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 5221 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 5281 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt tccggaaatc aacctctgga 5341 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5401 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5461 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5521 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5581 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5641 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5701 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5761 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5821 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag 5881 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac 5941 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 6181 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 6241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 6301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 6361 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg 6421 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa 6481 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 7021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 7081 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 7141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7741 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 7801 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 7861 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 7921 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 7981 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct 8041 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 8101 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 8161 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 8221 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 8281 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 8341 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 8401 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 8461 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 8521 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 8581 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 8641 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 8701 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 8761 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 8821 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat 8881 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa 8941 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta 9001 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 9061 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat 9121 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 9181 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 9241 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc 9301 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 TTTTGGATTGAAGCCAATATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGT 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 GGGAACGGGGCGGGTGACGTAGTAGTGTGGCGGAAGTGTGATGTTGCAAGTGTGGCGGAA 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 CACATGTAAGCGACGGATGTGGCAAAAGTGACGTTTTTGGTGTGCGCCGGTGTACACAGG 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 AAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTAGTAAATTTGGGCGTAACCGAGT 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 AAGATTTGGCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTGAAATCTGAATAATTTTG 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 TGTTACTCATAGCGCGTAATACGGCAGACCTCAGCGCTAGATTATTGAAGCATTTATCAG 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTAACTATAACGGTCCTAAGGTA 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 GCGAAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAG 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 CAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGG 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 TACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACT 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 GAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCT 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 CTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTT 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 AAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGAC 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 TCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGC 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 GCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTC 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 GGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAA 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 TTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGG 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 GGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATA 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 AATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCC 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 TGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGA 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 CAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATC 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 AAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACA 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 AAAGTAAGACCACCGCACAGCAAGCCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAG 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 GGACATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTA 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGA 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 GCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACG 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTG 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 AGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTC 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 CAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGG 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 GGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 AAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAAC 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 AATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAAT 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 GAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACA 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 AATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 ATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCG 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 TTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAA 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 GGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGA 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 AAGCTTGGGATTCGAATTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAG 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 AATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACAAAAACAAATTACAAA 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 AATTCAAAATTTTCGGGTTTTTCGAACCTAGGGTTCCGCGTTACATAACTTACGGTAAAT 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 GGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTT 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAA 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 ACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTC 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 AATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCT 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 ACTTGGCAGTACATCTACGTTTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTG 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 ACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_3 rf(3)(2901,4397)>>> | CMV prom(2885,2966)>>> | | 2881 ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 GAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCC 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 ATAGAAGAACCGAGTTTAAACTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAG 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 AGCTAGCGATATCAACAAGTTTGTACAAAAAAGTTGGCATGTCAGCCAGAAAGGGCTATC 3120 TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACAGTCGGTCTTTCCCGATAG 3121 TGCTCCCTTCGCCAAATTATCCCACAACAATGTCATGCTCGGAGAGCCCCGCCGCGAACT 3180 ACGAGGGAAGCGGTTTAATAGGGTGTTGTTACAGTACGAGCCTCTCGGGGCGGCGCTTGA 3181 CTTTTTTGGTCGACTCGCTCATCAGCTCGGGCAGAGGCGAGGCAGGCGGCGGTGGTGGTG 3240 GAAAAAACCAGCTGAGCGAGTAGTCGAGCCCGTCTCCGCTCCGTCCGCCGCCACCACCAC 3241 GCGCGGGGGGCGGCGGCGGTGGCGGTTACTACGCCCACGGCGGGGTCTACCTGCCGCCCG 3300 CGCGCCCCCCGCCGCCGCCACCGCCAATGATGCGGGTGCCGCCCCAGATGGACGGCGGGC PstI | 3301 CCGCCGACCTGCCCTACGGGCTGCAGAGCTGCGGGCTCTTCCCCACGCTGGGCGGCAAGC 3360 GGCGGCTGGACGGGATGCCCGACGTCTCGACGCCCGAGAAGGGGTGCGACCCGCCGTTCG 3361 GCAATGAGGCAGCGTCGCCGGGCAGCGGTGGCGGTGGCGGGGGTCTAGGTCCCGGGGCGC 3420 CGTTACTCCGTCGCAGCGGCCCGTCGCCACCGCCACCGCCCCCAGATCCAGGGCCCCGCG 3421 ACGGCTACGGGCCCTCGCCCATAGACCTGTGGCTAGACGCGCCCCGGTCTTGCCGGATGG 3480 TGCCGATGCCCGGGAGCGGGTATCTGGACACCGATCTGCGCGGGGCCAGAACGGCCTACC 3481 AGCCGCCTGACGGGCCGCCGCCGCCGCCCCAGCAGCAGCCGCCGCCCCCGCCGCAACCAC 3540 TCGGCGGACTGCCCGGCGGCGGCGGCGGGGTCGTCGTCGGCGGCGGGGGCGGCGTTGGTG 3541 CCCAGCCAGCGCCGCAGGCCACCTCGTGCTCTTTCGCGCAGAACATCAAAGAAGAGAGCT 3600 GGGTCGGTCGCGGCGTCCGGTGGAGCACGAGAAAGCGCGTCTTGTAGTTTCTTCTCTCGA 3601 CCTACTGCCTCTACGACTCGGCGGACAAATGCCCCAAAGTCTCGGCCACCGCCGCCGAAC 3660 GGATGACGGAGATGCTGAGCCGCCTGTTTACGGGGTTTCAGAGCCGGTGGCGGCGGCTTG 3661 TGGCTCCCTTCCCGCGGGGCCCGCCGCCCGACGGCTGCGCCCTGGGCACCTCCAGCGGGG 3720 ACCGAGGGAAGGGCGCCCCGGGCGGCGGGCTGCCGACGCGGGACCCGTGGAGGTCGCCCC StuI | 3721 TGCCAGTGCCTGGCTACTTCCGCCTTTCTCAGGCCTACGGCACCGCCAAGGGCTATGGCA 3780 ACGGTCACGGACCGATGAAGGCGGAAAGAGTCCGGATGCCGTGGCGGTTCCCGATACCGT 3781 GCGGCGGCGGCGGCGCGCAGCAACTCGGGGCTGGCCCGTTCCCCGCGCAGCCCCCGGGGC 3840 CGCCGCCGCCGCCGCGCGTCGTTGAGCCCCGACCGGGCAAGGGGCGCGTCGGGGGCCCCG 3841 GCGGTTTCGATCTCCCGCCCGCGCTAGCCTCCGGCTCGGCCGATGCGGCCCGGAAGGAGC 3900 CGCCAAAGCTAGAGGGCGGGCGCGATCGGAGGCCGAGCCGGCTACGCCGGGCCTTCCTCG 3901 GAGCCCTCGATTCGCCGCCGCCCCCCACGCTGGCTTGCGGCAGCGGCGGGGGCTCGCAGG 3960 CTCGGGAGCTAAGCGGCGGCGGGGGGTGCGACCGAACGCCGTCGCCGCCCCCGAGCGTCC 3961 GCGACGAGGAGGCGCACGCGTCGTCCTCGGCCGCGGAGGAGCTCTCCCCGGCCCCTTCCG 4020 CGCTGCTCCTCCGCGTGCGCAGCAGGAGCCGGCGCCTCCTCGAGAGGGGCCGGGGAAGGC 4021 AGAGCAGCAAAGCCTCGCCGGAGAAGGATTCCCTGGGCAATTCCAAAGGTGAAAACGCAG 4080 TCTCGTCGTTTCGGAGCGGCCTCTTCCTAAGGGACCCGTTAAGGTTTCCACTTTTGCGTC 4081 CCAACTGGCTCACGGCAAAGAGTGGTCGGAAGAAGCGCTGCCCCTACACGAAGCACCAGA 4140 GGTTGACCGAGTGCCGTTTCTCACCAGCCTTCTTCGCGACGGGGATGTGCTTCGTGGTCT 4141 CACTGGAGCTGGAGAAGGAGTTTCTGTTCAATATGTACCTTACTCGAGAGCGGCGCCTAG 4200 GTGACCTCGACCTCTTCCTCAAAGACAAGTTATACATGGAATGAGCTCTCGCCGCGGATC 4201 AGATTAGCCGCAGCGTCCACCTCACGGACAGACAAGTGAAAATCTGGTTTCAGAACCGCA 4260 TCTAATCGGCGTCGCAGGTGGAGTGCCTGTCTGTTCACTTTTAGACCAAAGTCTTGGCGT BamHI | 4261 GGATGAAACTGAAGAAAATGAATCGAGAAAACCGGATCCGGGAGCTCACAGCCAACTTTA 4320 CCTACTTTGACTTCTTTTACTTAGCTCTTTTGGCCTAGGCCCTCGAGTGTCGGTTGAAAT 4321 ATTTTTCCTGCCCAACTTTCTTGTACAAAGTGGTTGATATCCTCGAGTACCCATACGACG 4380 TAAAAAGGACGGGTTGAAAGAACATGTTTCACCAACTATAGGAGCTCATGGGTATGCTGC SV40 prom(4412,4680)>>> | 4381 TCCCAGACTACGCTTGAGTTTAAACACGCGTGGTGTGGAAAGTCCCCAGGCTCCCCAGCA 4440 AGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGT 4441 GGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCA 4500 CCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGT 4501 GGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTC 4560 CCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAG SV40 origin(4579,4656)>>> | 4561 CCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCC 4620 GGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGG ORF_2 rf(2)(4646,5314)>>> | 4621 CATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTA 4680 GTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGAT puro marker(4715,5314)>>> | 4681 TTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCATGACCGAGTACAAGCCCACGGTGCG 4740 AAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGC 4741 CCTCGCCACCCGCGACGACGTCCCTCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGA 4800 GGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCT 4801 CTACCCCGCCACGCGCCACACCGTGGACCCGGACCGCCACATCGAGCGGGTCACCGAGCT 4860 GATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGA 4861 GCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGA 4920 CGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCT 4921 CGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC 4980 GCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCG 4981 CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGAT 5040 GCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTA 5041 GGAAGGGCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGG 5100 CCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCC 5101 CGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGA 5160 GCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCT 5161 GGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCC 5220 CCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGG 5221 CTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCG 5280 GAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGC 5281 CACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTCCGGAAATCAACCTCTGGA 5340 GTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCT 5341 TTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATG 5400 AATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATAC 5401 TGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTT 5460 ACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAA 5461 CTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAG 5520 GAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTC 5521 GCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGC 5580 CGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACG 5581 CACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGA 5640 GTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCT 5641 ACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAA 5700 TGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTT 5701 TTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCAC 5760 AAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTG 5761 CTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCT 5820 GACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGA 5821 TCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTCGCCTTCGCCCTCAG 5880 AGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTC delta_U3 other(5924,5976)>>> | 5881 ACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCCGGATGGAAGGGCTAATTCAC 5940 TGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTG HIV-1_5_LTR other(5977,6157)>>> | 5941 TCCCAACGAATACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATC 6000 AGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAG 6001 TGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTG 6060 ACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAAC 6061 CCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCC 6120 GGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGG 6121 CTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATT 6180 GAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAA 6181 ATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATT 6240 TAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAA 6241 GCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTT 6300 CGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAA 6301 TTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGG 6360 AAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACC 6361 CATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCATTATGGGTATTATGGGTCTG 6420 GTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGAC 6421 CATTAATGAATCGGCCAACGATCCCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA 6480 GTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTT 6481 TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG 6540 ATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCC 6541 CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG 6600 GACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCC 6601 GATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAG 6660 CTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTC pBR322 origin(6663,7282)<<< | 6661 GCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA 6720 CGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCT 6721 CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT 6780 GCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGA 6781 GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC 6840 CCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGG 6841 TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCG 6900 AAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGC 6901 GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC 6960 CACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCG 6961 TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA 7020 ACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGT 7021 CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG 7080 GACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTC 7081 TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCT 7140 AAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGA 7141 CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACC 7200 GACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGG 7201 ACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGA 7260 TGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCT 7261 TCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA 7320 AGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGT 7321 CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT 7380 GCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTA 7381 TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAC 7440 ATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATG 7441 CAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTT 7500 GTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAA 7501 GCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGT 7560 CGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCA 7561 GCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAG 7620 CGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTC 7621 CCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCT 7680 GGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGA 7681 ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT 7740 TAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAA 7741 GTTGAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGT 7800 CAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCA 7801 GCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAA 7860 CGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTT 7861 AGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTAT 7920 TCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATA NEOKAN prom(7935,7984)>>> | 7921 GGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCT 7980 CCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGA 7981 GCAAAGTAAACTGGATGGCTTTCTCGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCT 8040 CGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGA NTP_II marker(8076,8864)>>> | BclI ORF_4 rf(3)(8073,8867)>>> | | | 8041 CTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAG 8100 GACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTC 8101 GTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCG 8160 CAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGC 8161 GCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCA 8220 CGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGT 8221 AGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGC 8280 TCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCG 8281 TGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGG 8340 ACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCC 8341 ACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTG 8400 TGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGAC 8401 CCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTA 8460 GGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGAT 8461 CCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAG 8520 GGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTC 8521 CCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAAC 8580 GGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTG 8581 TGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCG 8640 ACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGC 8641 ATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTG 8700 TACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACAC 8701 GCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTG 8760 CGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGAC 8761 AAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCG 8820 TTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGC 8821 ATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTTTGTTAAAAT 8880 TAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTA 8881 TTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAACATCCCTTATAAA 8940 AAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTT f1 origin(8972,9277)<<< | 8941 TCAAAAGAATAGACCGCGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTA 9000 AGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGAT 9001 TTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCA 9060 AATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGT 9061 CTACGTGAACCATCACCCAAATCAAGTTTTTTGCGGTCGAGGTGCCGTAAAGCTCTAAAT 9120 GATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTA 9121 CGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCG 9180 GCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGC 9181 AGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC 9240 TCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAG 9241 ACGCTGCGCGTAACCACCACACCCGCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTC 9300 TGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAG 9301 GCCATTCAGGATCGAATTAATTCTTAATTAACATCATCAATAATATACCTT 9351 CGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9351 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4397 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3321..3326 /dbxref="REBASE:PstI" misc_binding 3751..3756 /dbxref="REBASE:StuI" misc_binding 4294..4299 /dbxref="REBASE:BamHI" Promoter 4412..4680 /gene="SV40 prom" Rep_Origin 4579..4656 /gene="SV40 origin" ORF 4646..5314 /sequence="ORF_2 rf(2)" Marker 4715..5314 /gene="puro marker" Other Gene 5924..5976 /gene="delta_U3 other" Other Gene 5977..6157 /gene="HIV-1_5_LTR other" Rep_Origin 6663..7282 /gene="pBR322 origin" Promoter 7935..7984 /gene="NEOKAN prom" misc_binding 8042..8047 /dbxref="REBASE:BclI" ORF 8073..8867 /sequence="ORF_4 rf(3)" Marker 8076..8864 /gene="NTP_II marker" Rep_Origin 8972..9277 /gene="f1 origin" BASE COUNT 2271 a 2426 c 2584 g 2070 t 0 others