pLenti-GIII-CMV-DIO-SV40-Puro
| Vector Name | pLenti-GIII-CMV-DIO-SV40-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg 3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag 3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga 3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt 3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac 3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat 3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt 4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc 4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga 4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac 4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga 4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg 4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc 5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac 5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc 5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag 5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccatgaccga 5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg tacgcaccct 5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg accgccacat 5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5821 ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg agcccgcgtg 5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc 6121 cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6661 tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg 6721 atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct 6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7141 gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt 7201 atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac 7261 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 8521 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag 8581 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa 8641 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc 8701 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg 8761 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat 8821 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac 8881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 8941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 9001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg 9061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 9121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 9181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 9241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 9301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 9361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc 9421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 9481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 9541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 9601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 9661 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 9721 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt 9781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 9841 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg 9901 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 9961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 10021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc 10081 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 10141 atatacctt
LOCUS dna 10149 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" Other Gene 3273..3392 /gene="attR1 other" Other Gene 3292..3392 /gene="attR2 other" Other Gene 3433..3738 /gene="ccdB other" Marker 4080..4739 /gene="CAT marker" ORF 4080..4739 /sequence="ORF_4 rf(5)" misc_binding 4521..4526 /dbxref="REBASE:EcoRI" misc_binding 4840..4847 /dbxref="REBASE:NotI" Other Gene 4848..4972 /gene="attR1 other" Other Gene 4848..4948 /gene="attR2 other" Other Gene 5096..5129 /gene="loxP other" misc_binding 5148..5153 /dbxref="REBASE:XbaI" Promoter 5210..5478 /gene="SV40 prom" Rep_Origin 5377..5454 /gene="SV40 origin" ORF 5444..6112 /sequence="ORF_2 rf(2)" misc_binding 5457..5469 /dbxref="REBASE:SfiI" Marker 5513..6112 /gene="puro marker" Other Gene 6722..6774 /gene="delta_U3 other" Other Gene 6775..6955 /gene="HIV-1_5_LTR other" Rep_Origin 7461..8080 /gene="pBR322 origin" Promoter 8733..8782 /gene="NEOKAN prom" misc_binding 8840..8845 /dbxref="REBASE:BclI" ORF 8871..9665 /sequence="ORF_3 rf(3)" Marker 8874..9662 /gene="NTP_II marker" Rep_Origin 9770..10075 /gene="f1 origin" BASE COUNT 2629 a 2473 c 2585 g 2462 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg 3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag 3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga 3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt 3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac 3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat 3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt 4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc 4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga 4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac 4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga 4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg 4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc 5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac 5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc 5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag 5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccatgaccga 5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg tacgcaccct 5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg accgccacat 5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5821 ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg agcccgcgtg 5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc 6121 cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6661 tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg 6721 atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct 6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7141 gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt 7201 atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac 7261 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 8521 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag 8581 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa 8641 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc 8701 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg 8761 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat 8821 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac 8881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 8941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 9001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg 9061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 9121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 9181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 9241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 9301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 9361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc 9421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 9481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 9541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 9601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 9661 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 9721 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt 9781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 9841 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg 9901 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 9961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 10021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc 10081 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata 10141 atatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC ClaI KpnI | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT 3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180 ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG loxP other(3218,3251)<<< | 3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240 TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT attR2 other(3292,3392)<<< | attR1 other(3273,3392)>>> | | 3241 tacgaagttatctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacg 3300 ATGCTTCAATAGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGC 3301 taaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataa 3360 ATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATT 3361 tactgtaaaacacaacatatccagtcactatggtcgacctgcagactggctgtgtataag 3420 ATGACATTTTGTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTC ccdB other(3433,3738)<<< | 3421 ggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttc 3480 CCTCGGACTGTAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAG 3481 gcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatc 3540 CGCCACCGACTCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAG 3541 ggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacggga 3600 CCACCAGTAGTACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCT 3601 gactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgt 3660 CTGAAATAGACTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCA 3661 gtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggt 3720 CAGTTATTATAGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCA 3721 gtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcattt 3780 CATTTGGAATTTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAA 3781 caataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcac 3840 GTTATTTGGCCCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTG 3841 gcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcat 3900 CGTCTGCTGCCCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTA 3901 atatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcat 3960 TATACGGAACTCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTA 3961 agcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaa 4020 TCGTATGGAGAAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTT 4021 aaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccagatctt 4080 TTTAGTCGCGCGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAA ORF_4 rf(5)(4080,4739)<<< | CAT marker(4080,4739)<<< | 4081 tacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgaca 4140 ATGCGGGGCGGGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGT 4141 tggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcg 4200 ACCTTCGGTAGTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGC 4201 ccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggcc 4260 GGAACGCATATTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGG 4261 acgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc 4320 TGCAAATTTAGTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAG 4321 tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaa 4380 AGTTATTTGGGAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTT 4381 tatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtt 4440 ATATACACATCTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAA 4441 tcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctca 4500 AGTCAAACGAGTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGT EcoRI | 4501 ccgtctttcattgccatacggaattccggatgagcattcatcaggcgggcaagaatgtga 4560 GGCAGAAAGTAACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACT 4561 ataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata 4620 TATTTCCGGCCTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTAT 4621 tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgt 4680 AGGTCGACTTGCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACA 4681 tctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccatt 4740 AGAAATGCTACGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAA 4741 ttagcttccttagctcctgaaaatctcgacggatcctaactcaaaatccacacattatac 4800 AATCGAAGGAATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATG attR1 other(4848,4972)<<< | NotI attR2 other(4848,4948)>>> | | 4801 gagccggaagcataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactgga 4860 CTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCT 4861 tatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatat 4920 ATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATA 4921 tgatatttatatcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcg 4980 ACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGC 4981 ctagccaattgataacttcgtataggatactttatacgaagttatcattgggattcttcc 5040 GATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGG loxP other(5096,5129)>>> | 5041 tattttgatccaagcatcaccatcgaccctctagtccagatctcaccatcgacccataac 5100 ATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTG XbaI | 5101 ttcgtatagcatacattatacgaagttatatggtgagcaagggcgattctagactgcagc 5160 AAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTGACGTCG SV40 prom(5210,5478)>>> | 5161 tcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaaag 5220 AGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTC 5221 tccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaacc 5280 AGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGG 5281 aggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaat 5340 TCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTA SV40 origin(5377,5454)>>> | 5341 tagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagt 5400 ATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCA ORF_2 rf(2)(5444,6112)>>> | 5401 tccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggcc 5460 AGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGG SfiI puro marker(5513,6112)>>> | | 5461 gcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgaccga 5520 CGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCT 5521 gtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccct 5580 CATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGA 5581 cgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccacat 5640 GCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTA 5641 cgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaa 5700 GCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTT 5701 ggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcga 5760 CCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCT 5761 agcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggct 5820 TCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGA 5821 ggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtg 5880 CCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCAC 5881 gttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgt 5940 CAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCA 5941 cgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctc 6000 GCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAG 6001 cgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcga 6060 GCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCT 6061 ggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttc 6120 CCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAG 6121 cggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgt 6180 GCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACA 6181 tgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttc 6240 ACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAG 6241 ccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgagga 6300 GGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCT 6301 gttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccc 6360 CAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGG 6361 cactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccct 6420 GTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGA 6421 ccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcg 6480 GGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGC 6481 gctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggct 6540 CGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGA 6541 gctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggc 6600 CGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCG 6601 cctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcg 6660 GGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGC 6661 tctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccgg 6720 AGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCC delta_U3 other(6722,6774)>>> HIV-1_5_LTR other(6775,6955)>>> | | 6721 atggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtct 6780 TACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGA 6781 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 6840 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 6841 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 6900 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 6901 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagt 6960 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCA 6961 agttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagt 7020 TCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCA 7021 gagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaat 7080 CTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTA 7081 ttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaat 7140 AAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTA 7141 gtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcatt 7200 CATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAA 7201 atgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcac 7260 TACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTG 7261 agatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcg 7320 TCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGC 7321 ctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgg 7380 GACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCC 7381 ttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaag 7440 AATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTC pBR322 origin(7461,8080)<<< | 7441 gccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgac 7500 CGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTG 7501 gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaga 7560 CTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCT 7561 taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctt 7620 ATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAA 7621 accggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgc 7680 TGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCG 7681 tgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccc 7740 ACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGG 7741 cccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggta 7800 GGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCAT 7801 agacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat 7860 TCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATA 7861 gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggaca 7920 CATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGT 7921 gtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctct 7980 CATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGA 7981 tgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatt 8040 ACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAA 8041 acgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgct 8100 TGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGA 8101 cagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttc 8160 GTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAG 8161 acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaa 8220 TGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATT 8221 acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtcta 8280 TGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGAT 8281 tttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggc 8340 AAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCG 8341 ttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat 8400 AATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTA 8401 ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttta 8460 AATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAAT 8461 tccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtt 8520 AGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAA 8521 aatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaag 8580 TTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTC 8581 ccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaa 8640 GGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTT 8641 gggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagc 8700 CCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCG NEOKAN prom(8733,8782)>>> | 8701 tagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctg 8760 ATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGAC 8761 gtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgat 8820 CATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTA NTP_II marker(8874,9662)>>> | BclI ORF_3 rf(3)(8871,9665)>>> | | | 8821 ggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaac 8880 CCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTG 8881 aagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgact 8940 TTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGA 8941 gggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggc 9000 CCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCG 9001 gcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgagg 9060 CGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCC 9061 cagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttg 9120 GTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAAC 9121 tcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgt 9180 AGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACA 9181 catctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgc 9240 GTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACG 9241 atacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgag 9300 TATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTC 9301 cacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcagg 9360 GTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCC 9361 ggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatc 9420 CCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAG 9421 tcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctttt 9480 AGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAA 9481 ctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttgg 9540 GACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACC 9541 ctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgcttt 9600 GATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAA 9601 acggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttct 9660 TGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGA 9661 tctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatc 9720 AGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAG f1 origin(9770,10075)<<< | 9721 ggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtt 9780 CCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAA 9781 tggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtc 9840 ACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAG 9841 tatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgagg 9900 ATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCC 9901 tgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgggga 9960 ACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCT 9961 aagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcg 10020 TTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGC 10021 ctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgc 10080 GACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCG 10081 tacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaata 10140 ATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTAT 10141 atatacctt 10149 TATATGGAA
LOCUS dna 10149 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" Other Gene 3273..3392 /gene="attR1 other" Other Gene 3292..3392 /gene="attR2 other" Other Gene 3433..3738 /gene="ccdB other" Marker 4080..4739 /gene="CAT marker" ORF 4080..4739 /sequence="ORF_4 rf(5)" misc_binding 4521..4526 /dbxref="REBASE:EcoRI" misc_binding 4840..4847 /dbxref="REBASE:NotI" Other Gene 4848..4972 /gene="attR1 other" Other Gene 4848..4948 /gene="attR2 other" Other Gene 5096..5129 /gene="loxP other" misc_binding 5148..5153 /dbxref="REBASE:XbaI" Promoter 5210..5478 /gene="SV40 prom" Rep_Origin 5377..5454 /gene="SV40 origin" ORF 5444..6112 /sequence="ORF_2 rf(2)" misc_binding 5457..5469 /dbxref="REBASE:SfiI" Marker 5513..6112 /gene="puro marker" Other Gene 6722..6774 /gene="delta_U3 other" Other Gene 6775..6955 /gene="HIV-1_5_LTR other" Rep_Origin 7461..8080 /gene="pBR322 origin" Promoter 8733..8782 /gene="NEOKAN prom" misc_binding 8840..8845 /dbxref="REBASE:BclI" ORF 8871..9665 /sequence="ORF_3 rf(3)" Marker 8874..9662 /gene="NTP_II marker" Rep_Origin 9770..10075 /gene="f1 origin" BASE COUNT 2629 a 2473 c 2585 g 2462 t 0 others