pLenti-GIII-CMV-DIO-SV40-GFP

Vector Name pLenti-GIII-CMV-DIO-SV40-GFP
VectorType Lentiviral Vector
Antibiotic Information Kanamycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg 3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag 3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga 3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt 3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac 3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat 3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt 4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc 4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga 4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac 4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga 4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg 4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc 5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac 5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc 5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag 5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccgccaccat 5521 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 5581 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 5641 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 5701 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 5761 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 5821 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 5881 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 5941 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 6001 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 6061 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 6121 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 6181 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 6241 cggcaccgcc ggacccggct ccaccggatc tcgctgaacg cgttccggaa atcaacctct 6301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct 6841 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt 6901 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 6961 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 7021 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 7081 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 7141 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 7201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7321 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt 7381 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga 7441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 7501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 7561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 8041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 8101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 8161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8521 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8701 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac 8761 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg 8821 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt 8881 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc 8941 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa 9001 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 9061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 9121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 9181 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 9241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 9301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 9361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 9421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 9481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 9541 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 9601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 9661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 9721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 9781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa 9841 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat 9901 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca 9961 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 10021 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta 10081 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 10141 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 10201 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca 10261 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
                            LOCUS dna 10314 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" Other Gene 3273..3392 /gene="attR1 other" Other Gene 3292..3392 /gene="attR2 other" Other Gene 3433..3738 /gene="ccdB other" Marker 4080..4739 /gene="CAT marker" ORF 4080..4739 /sequence="ORF_4 rf(5)" misc_binding 4521..4526 /dbxref="REBASE:EcoRI" misc_binding 4840..4847 /dbxref="REBASE:NotI" Other Gene 4848..4972 /gene="attR1 other" Other Gene 4848..4948 /gene="attR2 other" Other Gene 5096..5129 /gene="loxP other" misc_binding 5148..5153 /dbxref="REBASE:XbaI" Promoter 5210..5478 /gene="SV40 prom" Rep_Origin 5377..5454 /gene="SV40 origin" ORF 5444..6277 /sequence="ORF_2 rf(2)" misc_binding 5457..5469 /dbxref="REBASE:SfiI" misc_binding 6103..6108 /dbxref="REBASE:ApaI" Other Gene 6887..6939 /gene="delta_U3 other" Other Gene 6940..7120 /gene="HIV-1_5_LTR other" Rep_Origin 7626..8245 /gene="pBR322 origin" Promoter 8898..8947 /gene="NEOKAN prom" misc_binding 9005..9010 /dbxref="REBASE:BclI" ORF 9036..9830 /sequence="ORF_3 rf(3)" Marker 9039..9827 /gene="NTP_II marker" Rep_Origin 9935..10240 /gene="f1 origin" BASE COUNT 2698 a 2516 c 2603 g 2497 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg 3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag 3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga 3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt 3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac 3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat 3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt 4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc 4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga 4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac 4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga 4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg 4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc 5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac 5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc 5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag 5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccgccaccat 5521 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 5581 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 5641 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 5701 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 5761 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 5821 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 5881 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 5941 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 6001 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 6061 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 6121 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 6181 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 6241 cggcaccgcc ggacccggct ccaccggatc tcgctgaacg cgttccggaa atcaacctct 6301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct 6841 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt 6901 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 6961 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 7021 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 7081 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 7141 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 7201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7321 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt 7381 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga 7441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 7501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 7561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 8041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 8101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 8161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8521 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8701 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac 8761 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg 8821 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt 8881 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc 8941 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa 9001 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 9061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 9121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 9181 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 9241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 9301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 9361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 9421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 9481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 9541 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 9601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 9661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 9721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 9781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa 9841 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat 9901 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca 9961 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 10021 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta 10081 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 10141 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 10201 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca 10261 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt //
                        
***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC ClaI KpnI | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT 3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180 ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG loxP other(3218,3251)<<< | 3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240 TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT attR2 other(3292,3392)<<< | attR1 other(3273,3392)>>> | | 3241 tacgaagttatctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacg 3300 ATGCTTCAATAGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGC 3301 taaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataa 3360 ATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATT 3361 tactgtaaaacacaacatatccagtcactatggtcgacctgcagactggctgtgtataag 3420 ATGACATTTTGTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTC ccdB other(3433,3738)<<< | 3421 ggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttc 3480 CCTCGGACTGTAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAG 3481 gcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatc 3540 CGCCACCGACTCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAG 3541 ggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacggga 3600 CCACCAGTAGTACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCT 3601 gactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgt 3660 CTGAAATAGACTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCA 3661 gtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggt 3720 CAGTTATTATAGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCA 3721 gtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcattt 3780 CATTTGGAATTTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAA 3781 caataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcac 3840 GTTATTTGGCCCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTG 3841 gcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcat 3900 CGTCTGCTGCCCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTA 3901 atatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcat 3960 TATACGGAACTCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTA 3961 agcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaa 4020 TCGTATGGAGAAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTT 4021 aaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccagatctt 4080 TTTAGTCGCGCGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAA ORF_4 rf(5)(4080,4739)<<< | CAT marker(4080,4739)<<< | 4081 tacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgaca 4140 ATGCGGGGCGGGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGT 4141 tggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcg 4200 ACCTTCGGTAGTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGC 4201 ccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggcc 4260 GGAACGCATATTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGG 4261 acgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc 4320 TGCAAATTTAGTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAG 4321 tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaa 4380 AGTTATTTGGGAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTT 4381 tatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtt 4440 ATATACACATCTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAA 4441 tcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctca 4500 AGTCAAACGAGTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGT EcoRI | 4501 ccgtctttcattgccatacggaattccggatgagcattcatcaggcgggcaagaatgtga 4560 GGCAGAAAGTAACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACT 4561 ataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata 4620 TATTTCCGGCCTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTAT 4621 tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgt 4680 AGGTCGACTTGCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACA 4681 tctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccatt 4740 AGAAATGCTACGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAA 4741 ttagcttccttagctcctgaaaatctcgacggatcctaactcaaaatccacacattatac 4800 AATCGAAGGAATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATG attR1 other(4848,4972)<<< | NotI attR2 other(4848,4948)>>> | | 4801 gagccggaagcataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactgga 4860 CTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCT 4861 tatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatat 4920 ATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATA 4921 tgatatttatatcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcg 4980 ACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGC 4981 ctagccaattgataacttcgtataggatactttatacgaagttatcattgggattcttcc 5040 GATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGG loxP other(5096,5129)>>> | 5041 tattttgatccaagcatcaccatcgaccctctagtccagatctcaccatcgacccataac 5100 ATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTG XbaI | 5101 ttcgtatagcatacattatacgaagttatatggtgagcaagggcgattctagactgcagc 5160 AAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTGACGTCG SV40 prom(5210,5478)>>> | 5161 tcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaaag 5220 AGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTC 5221 tccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaacc 5280 AGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGG 5281 aggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaat 5340 TCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTA SV40 origin(5377,5454)>>> | 5341 tagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagt 5400 ATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCA ORF_2 rf(2)(5444,6277)>>> | 5401 tccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggcc 5460 AGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGG SfiI | 5461 gcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccgccaccAt 5520 CGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGCGGTGGTA 5521 ggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccct 5580 CCTCTCGCTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCGTAGTGGCCGTGGGA 5581 gaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcat 5640 CTTGCCGCACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGGTTCGTCCCGGCGTA 5641 gaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagcca 5700 CTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGGATGGACGACTCGGT 5701 cgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaacccctt 5760 GCACTACCCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCGATGCTCTTGGGGAA 5761 cctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacgg 5820 GGACGTGCGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTCTTCATGCTCCTGCC 5821 cggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgactt 5880 GCCGCACGACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCGCACTAGCCGCTGAA 5881 caaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccg 5940 GTTCCACCACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGGCTGTTCTAGTAGGC 5941 cagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagctt 6000 GTCGTTGCGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCACGACCACCCGTCGAA 6001 cgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacat 6060 GCGGGCGTGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCACCACCTGTCGGTGTA ApaI | 6061 gcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgcctt 6120 CGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCGGGGTACAAGCGGAA 6121 ccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgc 6180 GGCGGCGCACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCACCTCATGGTCGTGCG 6181 cttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtgga 6240 GAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGGTTAAGACGGCACCT 6241 cggcaccgccggacccggctccaccggatctcgctgaacgcgttccggaaatcaacctct 6300 GCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGCGACTTGCGCAAGGCCTTTAGTTGGAGA 6301 ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgct 6360 CCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGA 6361 atgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcat 6420 TACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTA 6421 tttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgt 6480 AAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACA 6481 caggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcat 6540 GTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTA 6541 tgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggc 6600 ACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCG 6601 ggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactga 6660 CCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACT 6661 caattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgc 6720 GTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACG 6721 cacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcgga 6780 GTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCT 6781 ccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccct 6840 GGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGA delta_U3 other(6887,6939)>>> | 6841 cagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaatt 6900 GTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAA HIV-1_5_LTR other(6940,7120)>>> | 6901 cactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccag 6960 GTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTC 6961 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 7020 TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG 7021 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 7080 AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT 7081 tccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatctt 7140 AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAA 7141 attattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgttt 7200 TAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAA 7201 attgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagca 7260 TAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGT 7261 tttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtc 7320 AAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAG 7321 tggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggt 7380 ACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCA 7381 ctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggaga 7440 GACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCT 7441 aaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt 7500 TTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAA 7501 cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatca 7560 GCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGT 7561 ggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaa 7620 CCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTT pBR322 origin(7626,8245)<<< | 7621 aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat 7680 TTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTA 7681 cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc 7740 GCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGG 7741 cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc 7800 GGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGG 7801 gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagt 7860 CGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCA 7861 tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac 7920 AGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTG 7921 cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg 7980 GCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGC 7981 ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca 8040 GGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGT 8041 gagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgc 8100 CTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACG 8101 gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa 8160 CGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTT 8161 accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaa 8220 TGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTT 8221 ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac 8280 CCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTG 8281 tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta 8340 AGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAT 8341 aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt 8400 TTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCA 8401 taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccata 8460 ATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTAT 8461 gttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc 8520 CAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGG 8521 agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaac 8580 TCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTG 8581 cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag 8640 GTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTC 8641 tctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac 8700 AGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTG 8701 gttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaac 8760 CAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTG 8761 ggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcg 8820 CCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGC 8821 caaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttt 8880 GTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAA NEOKAN prom(8898,8947)>>> | 8881 tatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc 8940 ATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCG 8941 cctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaa 9000 GGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTT NTP_II marker(9039,9827)>>> | BclI ORF_3 rf(3)(9036,9830)>>> | | | 9001 gctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacg 9060 CGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGC 9061 caggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaa 9120 GTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTT 9121 tcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttg 9180 AGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAAC 9181 tcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgt 9240 AGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCA 9241 ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaa 9300 CCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTT 9301 gggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctc 9360 CCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAG 9361 ctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccgg 9420 GACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCC 9421 ctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgg 9480 GATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACC 9481 aagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg 9540 TTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGC 9541 aactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatg 9600 TTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTAC 9601 gcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgact 9660 CGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGA 9661 gtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattg 9720 CACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAAC 9721 ctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctc 9780 GACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAG 9781 ccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaa 9840 GGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATT 9841 aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttat 9900 TTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATA f1 origin(9935,10240)<<< | 9901 aaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcca 9960 TTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGT 9961 ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc 10020 GATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCG 10021 ccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctcta 10080 GGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGAT 10081 aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg 10140 TTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCAC 10141 gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg 10200 CGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGC 10201 gtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcca 10260 CAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGT 10261 ttcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10314 AAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
                            LOCUS dna 10314 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" Other Gene 3273..3392 /gene="attR1 other" Other Gene 3292..3392 /gene="attR2 other" Other Gene 3433..3738 /gene="ccdB other" Marker 4080..4739 /gene="CAT marker" ORF 4080..4739 /sequence="ORF_4 rf(5)" misc_binding 4521..4526 /dbxref="REBASE:EcoRI" misc_binding 4840..4847 /dbxref="REBASE:NotI" Other Gene 4848..4972 /gene="attR1 other" Other Gene 4848..4948 /gene="attR2 other" Other Gene 5096..5129 /gene="loxP other" misc_binding 5148..5153 /dbxref="REBASE:XbaI" Promoter 5210..5478 /gene="SV40 prom" Rep_Origin 5377..5454 /gene="SV40 origin" ORF 5444..6277 /sequence="ORF_2 rf(2)" misc_binding 5457..5469 /dbxref="REBASE:SfiI" misc_binding 6103..6108 /dbxref="REBASE:ApaI" Other Gene 6887..6939 /gene="delta_U3 other" Other Gene 6940..7120 /gene="HIV-1_5_LTR other" Rep_Origin 7626..8245 /gene="pBR322 origin" Promoter 8898..8947 /gene="NEOKAN prom" misc_binding 9005..9010 /dbxref="REBASE:BclI" ORF 9036..9830 /sequence="ORF_3 rf(3)" Marker 9039..9827 /gene="NTP_II marker" Rep_Origin 9935..10240 /gene="f1 origin" BASE COUNT 2698 a 2516 c 2603 g 2497 t 0 others