pLenti-Easy-HA
| Vector Name | pLenti-Easy-HA |
| VectorType | Lentiviral vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgt
agtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttgg
tgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt
aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaat
acggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt
agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta
gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagcc
agtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttg
accgacaattaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa
ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacggg
tctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacg
ggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc
ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtc
agtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcag
gactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag
gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggtta
aggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcc
tggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac
ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagcttta
gacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcgccgctgatcttcagacctggaggaggaga
tatgagggacaattgagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc
aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaa
gcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaat
ttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct
ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctg
tgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaa
attaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt
ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatga
tagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacca
ttatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga
cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagttccgcgttacataacttacgg
taaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcca
atagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat
gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggact
ttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgt
ggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca
acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctata
taagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgg
ctagcgtttaaacttaagcttggtaccctcgagcctagggggcccagtacttctagaccgcggactagtgcggccgctgg
atccgaattctacccatacgacgtcccagactacgcttgaAGATccggctgtggaatgtgtgtcagttagggtgtggaaa
gtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccag
gctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatc
ccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggc
cgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagcgaccagatctaatgcgtgctagC
ACGCATTAGATCGGTCGCTAGCGACCAGATCTAATGCGTGCTAGCGACCAGATCTAATGCGTGCTAGGCttttgcaaaaa
gcttgggctgcaggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgca
cgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccg
ccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcag
gacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggg
aagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcca
tcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatc
gagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagc
cgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccga
atatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacata
gcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgc
tcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgatatctagcg
accagatctaatgcgtgctagaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaat
tcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggtctagcgaccagatctaatgcgtgctag
gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtg
tgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtag
tagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgggcctga
ccagatctaatgcgtaggccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgccc
gggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatga
ggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggatt
gggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgc
atctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacga
tcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactc
gctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg
ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttc
cataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaag
ataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct
ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaag
ctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggt
aagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt
tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttc
ggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagat
tacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac
gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatca
atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtct
atttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg
ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgc
agaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagt
taatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtg
ctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgca
gtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctct
ggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctc
tgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtgg
agaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcagggg
cgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggct
ggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaag
tgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctg
catacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagc
cggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcga
gcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttt
tctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctga
agagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttct
atcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaat
cggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactat
taaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaa
tcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgggg
aaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtca
cgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaat
tcttaattaacatcatcaataatatacctt
LOCUS dna 8119 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
regulatory_sequence..1151
/gene="5'-LTR"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_2 rf(2)"
promoter 2450..3020
/gene="CMV "
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
misc_binding 3062..3067
/dbxref="REBASE:KpnI"
misc_binding 3068..3073
/dbxref="REBASE:XhoI"
misc_binding 3080..3085
/dbxref="REBASE:ApaI"
misc_binding 3092..3097
/dbxref="REBASE:XbaI"
misc_binding 3110..3117
/dbxref="REBASE:NotI"
misc_binding 3119..3124
/dbxref="REBASE:BamHI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
tag 3130..3160
/gene="HA "
Regulatory_Seq 3167..3403
/gene="SV40ER reg"
Promoter 3190..3458
/gene="SV40 prom"
Rep_Origin 3357..3434
/gene="SV40 origin"
misc_binding 3437..3449
/dbxref="REBASE:SfiI"
misc_binding 3488..3493
/dbxref="REBASE:StuI"
origin_of_replication.4379
/gene="Neo/Kan"
ORF 3587..4381
/sequence="ORF_3 rf(2)"
misc_binding 4399..4404
/dbxref="REBASE:EcoRV"
Other Gene 4477..4529
/gene="delta_U3 other"
origin_of_replication.4790
/gene="3'-LTR"
misc_binding 4887..4892
/dbxref="REBASE:SmaI"
misc_binding 4887..4892
/dbxref="REBASE:XmaI"
Rep_Origin 5431..6050
/gene="pBR322 origin"
Promoter 6703..6752
/gene="NEOKAN prom"
ORF 6841..7635
/sequence="ORF_1 rf(1)"
Rep_Origin 7740..8045
/gene="f1 origin"
BASE COUNT 2066 a 1918 c 2241 g 1894 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct
3061 tggtaccctc gagcctaggg ggcccagtac ttctagaccg cggactagtg cggccgctgg
3121 atccgaattc tacccatacg acgtcccaga ctacgcttga tccccggctg tggaatgtgt
3181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
3241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
3301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
3361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
3421 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
3481 tttttggagg cctagtgacc agatctaatg cgtgctagtt ttgcaaaaag cttgggctgc
3541 aggtcgaggc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
3601 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3661 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3721 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
3781 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3841 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3901 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3961 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
4021 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
4081 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
4141 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
4201 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
4261 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
4321 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
4381 agcgggactc tggggttcga tatctagcga ccagatctaa tgcgtgctag aaggcagctg
4441 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
4501 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gtctagcgac cagatctaat
4561 gcgtgctagg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
4621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
4681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
4741 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
4801 tgggcctgac cagatctaat gcgtaggccg tttaaaccgc tgatcagcct cgactgtgcc
4861 ttctagttgc cagccatctg ttgttgcccg ggcgcgatcg ctgcccctcc cccgtgcctt
4921 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
4981 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
5041 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
5101 aggcggaaag aaccagcaga tcgatctgca tctatgtcgg gtgcggagaa agaggtaatg
5161 aaatggcatt atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga
5221 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
5281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
5341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
5401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
5461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
5521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
5581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
5641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
5701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
5761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
5821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
5881 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
5941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
6001 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
6061 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
6121 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
6181 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
6241 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
6301 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
6361 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
6421 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
6481 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc
6541 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
6601 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
6661 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
6721 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca
6781 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
6841 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
6901 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
6961 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
7021 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
7081 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
7141 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
7201 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
7261 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
7321 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
7381 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
7441 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
7501 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
7561 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
7621 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
7681 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt
7741 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
7801 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
7861 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc
7921 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
7981 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt
8041 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac
8101 atcatcaata atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtct 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAATTACATCAGA
5'-LTR(669,1151)>>>
|
661 tatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa 720
ATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTT
721 ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgcctta 780
CCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGCACGGAAT
781 ttaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgc 840
AATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGGCGTAACG
HIV-1_5_LTR other(880,1060)>>>
|
841 agagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccag 900
TCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACCAATCTGGTC
901 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 960
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG
961 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 1020
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT
1021 tccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggact 1080
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGA
1081 tgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgc 1140
ACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCG
HIV-1_psi_pack other(1171,1215)>>>
|
1141 gcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag 1200
CGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTC
1201 gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgc 1260
CGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCG
NruI
|
1261 gatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatag 1320
CTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATATC
1321 tatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcag 1380
ATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTC
1381 aaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac 1440
TTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTG
1441 ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataa 1500
AATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCTCTATT
1501 aagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccg 1560
TTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGC
1561 cacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaag 1620
GTGTCGTTCGCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACTCTTC
1621 tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc 1680
ACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGTGGTTCCG
RRE reg(1723,1956)>>>
|
1681 aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgg 1740
TTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGAACC
ORF_2 rf(2)(1766,2449)>>>
|
1741 gttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggc 1800
CAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCG
1801 cagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc 1860
GTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGATAACTCCG
1861 gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct 1920
CGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTTCTTAGGA
1921 ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaa 1980
CCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTT
1981 actcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaaca 2040
TGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAGACCTTGT
2041 gatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt 2100
CTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTGTTCGAA
2101 aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt 2160
TTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAA
2161 ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggta 2220
CCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGACACCAT
2221 tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgt 2280
ATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAAACGACA
2281 actttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct 2340
TGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGA
2341 cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga 2400
GGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCTCTCTCT
CMV (2450,3020)>>>
|
2401 cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagtt 2460
GTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTATTCGAACCCTCAA
2461 ccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgccc 2520
GGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGG
2521 attgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacg 2580
TAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGC
NdeI
|
2581 tcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat 2640
AGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATA
2641 gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgccca 2700
CGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGT
2701 gtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctat 2760
CATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATA
2761 taccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacg 2820
ATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGC
2821 gggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca 2880
CCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGT
CMV prom(2911,2992)>>>
|
2881 acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcg 2940
TGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGC
2941 tgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggag 3000
ACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTC
3001 acgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagct 3060
TGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCGATCGCAAATTTGAATTCGA
XhoI
|
KpnI ApaI XbaI NotI
| | | | |
3061 tggtaccctcgagcctagggggcccagtacttctagaccgcggactagtgcggccgctgg 3120
ACCATGGGAGCTCGGATCCCCCGGGTCATGAAGATCTGGCGCCTGATCACGCCGGCGACC
HA (3130,3160)>>>
|
BamHI EcoRI SV40ER reg(3167,3403)<<<
| | | |
3121 atccgaattctacccatacgacgtcccagactacgcttgatccccggctgtggaatgtgt 3180
TAGGCTTAAGATGGGTATGCTGCAGGGTCTGATGCGAACTAGGGGCCGACACCTTACACA
SV40 prom(3190,3458)>>>
|
3181 gtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgc 3240
CAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACG
3241 atctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagta 3300
TAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCAT
SV40 origin(3357,3434)>>>
|
3301 tgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcc 3360
ACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGG
3361 cgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttttta 3420
GCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAAT
SfiI
|
3421 tttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggct 3480
AAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGA
StuI
|
3481 tttttggaggcctagtgaccagatctaatgcgtgctagttttgcaaaaagcttgggctgc 3540
AAAAACCTCCGGATCACTGGTCTAGATTACGCACGATCAAAACGTTTTTCGAACCCGACG
ORF_3 rf(2)(3587,4381)>>>
|
Neo/Kan(3584,4379)>>>
| |
3541 aggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaaga 3600
TCCAGCTCCGCCTAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCT
3601 tggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggc 3660
ACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCG
3661 acaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgccc 3720
TGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGG
3721 ggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagc 3780
CCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCG
3781 gcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcac 3840
CGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTG
3841 tgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatc 3900
ACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAG
3901 tcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatac 3960
AGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATG
3961 gcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacg 4020
CGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGC
4021 tactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggct 4080
ATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGA
4081 cgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgt 4140
GCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGCA
4141 cgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctgg 4200
GCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACC
4201 attcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctac 4260
TAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATG
4261 ccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacgg 4320
GGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCC
4321 tatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctg 4380
ATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGAC
EcoRV
|
4381 agcgggactctggggttcgatatctagcgaccagatctaatgcgtgctagaaggcagctg 4440
TCGCCCTGAGACCCCAAGCTATAGATCGCTGGTCTAGATTACGCACGATCTTCCGTCGAC
delta_U3 other(4477,4529)>>>
|
4441 tagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaac 4500
ATCTAGAATCGGTGAAAAATTTTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTG
3'-LTR(4500,4790)>>>
|
4501 gaagacaagatctgctttttgcttgtactgggtctctctggtctagcgaccagatctaat 4560
CTTCTGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAGATCGCTGGTCTAGATTA
4561 gcgtgctaggagcctgggagctctctggctaactagggaacccactgcttaagcctcaat 4620
CGCACGATCCTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTA
4621 aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaact 4680
TTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGA
4681 agagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtc 4740
TCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAG
4741 atcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaact 4800
TAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGA
4801 tgggcctgaccagatctaatgcgtaggccgtttaaaccgctgatcagcctcgactgtgcc 4860
ACCCGGACTGGTCTAGATTACGCATCCGGCAAATTTGGCGACTAGTCGGAGCTGACACGG
SmaI
|
XmaI
| |
4861 ttctagttgccagccatctgttgttgcccgggcgcgatcgctgcccctcccccgtgcctt 4920
AAGATCAACGGTCGGTAGACAACAACGGGCCCGCGCTAGCGACGGGGAGGGGGCACGGAA
4921 ccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcat 4980
GGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTA
4981 cgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagg 5040
GCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCC
5041 gggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctg 5100
CCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCCACCCGAGATACCGAAGAC
5101 aggcggaaagaaccagcagatcgatctgcatctatgtcgggtgcggagaaagaggtaatg 5160
TCCGCCTTTCTTGGTCGTCTAGCTAGACGTAGATACAGCCCACGCCTCTTTCTCCATTAC
5161 aaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtga 5220
TTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACT
5221 aataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgct 5280
TTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGA
5281 cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc 5340
GTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCG
5341 ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaagg 5400
CCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCC
pBR322 origin(5431,6050)<<<
|
5401 ccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccg 5460
GGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGC
5461 cccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagg 5520
GGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCC
5521 actataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac 5580
TGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTG
5581 cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctca 5640
GGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGT
5641 tagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgt 5700
ATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACA
5701 gcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtc 5760
CGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAG
5761 caacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag 5820
GTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTC
5821 agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac 5880
TCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTG
5881 tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagt 5940
ATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCA
5941 tggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaa 6000
ACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTT
6001 gcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg 6060
CGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCC
6061 gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa 6120
CAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTT
6121 aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat 6180
TTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATA
6181 atatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagc 6240
TATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCG
6241 gatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat 6300
CTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTA
6301 acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc 6360
TGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGG
6361 ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcc 6420
CCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGG
6421 tgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtag 6480
ACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATC
6481 ttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttc 6540
AAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAG
6541 acgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggct 6600
TGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGA
6601 atctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttaca 6660
TAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGT
NEOKAN prom(6703,6752)>>>
|
6661 tggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggg 6720
ACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCC
6721 gcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgcca 6780
CGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGT
6781 aggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgc 6840
TCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCG
ORF_1 rf(1)(6841,7635)>>>
|
6841 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattc 6900
TACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAG
6901 ggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtca 6960
CCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGT
6961 gcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactg 7020
CGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGAC
7021 caagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtg 7080
GTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACAC
7081 ctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcag 7140
GAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTC
7141 gatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatg 7200
CTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTAC
7201 cggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgc 7260
GCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCG
7261 atcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaa 7320
TAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTT
7321 gagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgac 7380
CTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTG
7381 ggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaat 7440
CCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTA
7441 ggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggac 7500
CCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTG
7501 atagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttc 7560
TATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAG
7561 ctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttctt 7620
GAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAA
7621 gacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaata 7680
CTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTAT
7681 ggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgt 7740
CCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACA
f1 origin(7740,8045)<<<
|
7741 tgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcg 7800
ACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGC
7801 aaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtttttt 7860
TTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAA
7861 gcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagc 7920
CGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCG
7921 ttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcggg 7980
AACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCC
7981 cgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgctt 8040
GCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAA
8041 aatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaac 8100
TTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTG
8101 atcatcaataatatacctt 8119
TAGTAGTTATTATATGGAA
5'-LTR
Start: 670 End: 1151
CMV Promoter
Start: 2451 End: 3020
Neomycin
Start: 3635 End: 4429
3'-LTR
Start: 4551 End: 4840