pLenti-EF1a-hTERT

Vector Name	pLenti-EF1a-hTERT
VectorType	Lentiviral Plasmid
Antibiotic Information	No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds.
Sequencing Primers	EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca 
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag 
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc 
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc 
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc 
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat 
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc 
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc 
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt 
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga 
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag 
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca 
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg 
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagcg atatcacaag tttgtacaaa 
3661 aaagttggca tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac 
3721 taccgcgagg tgctgccgct ggccacgttc gtgcggcgcc tggggcccca gggctggcgg 
3781 ctggtgcagc gcggggaccc ggcggctttc cgcgcgctgg tggcccagtg cctggtgtgc 
3841 gtgccctggg acgcacggcc gccccccgcc gccccctcct tccgccaggt gtcctgcctg 
3901 aaggagctgg tggcccgagt gctgcagagg ctgtgcgagc gcggcgcgaa gaacgtgctg 
3961 gccttcggct tcgcgctgct ggacggggcc cgcgggggcc cccccgaggc cttcaccacc 
4021 agcgtgcgca gctacctgcc caacacggtg accgacgcac tgcgggggag cggggcgtgg 
4081 gggctgctgc tgcgccgcgt gggcgacgac gtgctggttc acctgctggc acgctgcgcg 
4141 ctctttgtgc tggtggctcc cagctgcgcc taccaggtgt gcgggccgcc gctgtaccag 
4201 ctcggcgctg ccactcaggc ccggcccccg ccacacgcta gtggaccccg aaggcgtctg 
4261 ggatgcgaac gggcctggaa ccatagcgtc agggaggccg gggtccccct gggcctgcca 
4321 gccccgggtg cgaggaggcg cgggggcagt gccagccgaa gtctgccgtt gcccaagagg 
4381 cccaggcgtg gcgctgcccc tgagccggag cggacgcccg ttgggcaggg gtcctgggcc 
4441 cacccgggca ggacgcgtgg accgagtgac cgtggtttct gtgtggtgtc acctgccaga 
4501 cccgccgaag aagccacctc tttggagggt gcgctctctg gcacgcgcca ctcccaccca 
4561 tccgtgggcc gccagcacca cgcgggcccc ccatccacat cgcggccacc acgtccctgg 
4621 gacacgcctt gtcccccggt gtacgccgag accaagcact tcctctactc ctcaggcgac 
4681 aaggagcagc tgcggccctc cttcctactc agctctctga ggcccagcct gactggcgct 
4741 cggaggctcg tggagaccat ctttctgggt tccaggccct ggatgccagg gactccccgc 
4801 aggttgcccc gcctgcccca gcgctactgg caaatgcggc ccctgtttct ggagctgctt 
4861 gggaaccacg cgcagtgccc ctacggggtg ctcctcaaga cgcactgccc gctgcgagct 
4921 gcggtcaccc cagcagccgg tgtctgtgcc cgggagaagc cccagggctc tgtggcggcc 
4981 cccgaggagg aggacacaga cccccgtcgc ctggtgcagc tgctccgcca gcacagcagc 
5041 ccctggcagg tgtacggctt cgtgcgggcc tgcctgcgcc ggctggtgcc cccaggcctc 
5101 tggggctcca ggcacaacga acgccgcttc ctcaggaaca ccaagaagtt catctccctg 
5161 gggaagcatg ccaagctctc gctgcaggag ctgacgtgga agatgagcgt gcgggactgc 
5221 gcttggctgc gcaggagccc aggggttggc tgtgttccgg ccgcagagca ccgtctgcgt 
5281 gaggagatcc tggccaagtt cctgcactgg ctgatgagtg tgtacgtcgt cgagctgctc 
5341 aggtctttct tttatgtcac ggagaccacg tttcaaaaga acaggctctt tttctaccgg 
5401 aagagtgtct ggagcaagtt gcaaagcatt ggaatcagac agcacttgaa gagggtgcag 
5461 ctgcgggagc tgtcggaagc agaggtcagg cagcatcggg aagccaggcc cgccctgctg 
5521 acgtccagac tccgcttcat ccccaagcct gacgggctgc ggccgattgt gaacatggac 
5581 tacgtcgtgg gagccagaac gttccgcaga gaaaagaggg ccgagcgtct cacctcgagg 
5641 gtgaaggcac tgttcagcgt gctcaactac gagcgggcgc ggcgccccgg cctcctgggc 
5701 gcctctgtgc tgggcctgga cgatatccac agggcctggc gcaccttcgt gctgcgtgtg 
5761 cgggcccagg acccgccgcc tgagctgtac tttgtcaagg tggatgtgac gggcgcgtac 
5821 gacaccatcc cccaggacag gctcacggag gtcatcgcca gcatcatcaa accccagaac 
5881 acgtactgcg tgcgtcggta tgccgtggtc cagaaggccg cccatgggca cgtccgcaag 
5941 gccttcaaga gccacgtctc taccttgaca gacctccagc cgtacatgcg acagttcgtg 
6001 gctcacctgc aggagaccag cccgctgagg gatgccgtcg tcatcgagca gagctcctcc 
6061 ctgaatgagg ccagcagtgg cctcttcgac gtcttcctac gcttcatgtg ccaccacgcc 
6121 gtgcgcatca ggggcaagtc ctacgtccag tgccagggga tcccgcaggg ctccatcctc 
6181 tccacgctgc tctgcagcct gtgctacggc gacatggaga acaagctgtt tgcggggatt 
6241 cggcgggacg ggctgctcct gcgtttggtg gatgatttct tgttggtgac acctcacctc 
6301 acccacgcga aaaccttcct caggaccctg gtccgaggtg tccctgagta tggctgcgtg 
6361 gtgaacttgc ggaagacagt ggtgaacttc cctgtagaag acgaggccct gggtggcacg 
6421 gcttttgttc agatgccggc ccacggccta ttcccctggt gcggcctgct gctggatacc 
6481 cggaccctgg aggtgcagag cgactactcc agctatgccc ggacctccat cagagccagt 
6541 ctcaccttca accgcggctt caaggctggg aggaacatgc gtcgcaaact ctttggggtc 
6601 ttgcggctga agtgtcacag cctgtttctg gatttgcagg tgaacagcct ccagacggtg 
6661 tgcaccaaca tctacaagat cctcctgctg caggcgtaca ggtttcacgc atgtgtgctg 
6721 cagctcccat ttcatcagca agtttggaag aaccccacat ttttcctgcg cgtcatctct 
6781 gacacggcct ccctctgcta ctccatcctg aaagccaaga acgcagggat gtcgctgggg 
6841 gccaagggcg ccgccggccc tctgccctcc gaggccgtgc agtggctgtg ccaccaagca 
6901 ttcctgctca agctgactcg acaccgtgtc acctacgtgc cactcctggg gtcactcagg 
6961 acagcccaga cgcagctgag tcggaagctc ccggggacga cgctgactgc cctggaggcc 
7021 gcagccaacc cggcactgcc ctcagacttc aagaccatcc tggactgccc aactttcttg 
7081 tacaaagtgg tggatatctg aggatcctct agactgcagc tcgagtaccc atacgacgtc 
7141 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg 
7201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
7321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
7381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
7441 ccagaagtag tgaggaggct tttttggagg ccatgaccga gtacaagccc acggtgcgcc 
7501 tcgccacccg cgacgacgtc cctcgggccg tacgcaccct cgccgccgcg ttcgccgact 
7561 accccgccac gcgccacacc gtggacccgg accgccacat cgagcgggtc accgagctgc 
7621 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
7681 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
7741 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
7801 aagggctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
7861 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
7921 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
7981 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 
8041 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt 
8101 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
8161 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
8221 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
8281 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
8341 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
8401 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
8461 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
8521 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
8581 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac 
8641 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc 
8701 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 
8761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
8821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
8881 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 
8941 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 
9001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
9061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca 
9121 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 
9181 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 
9241 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
9301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
9361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
9421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
9481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
9541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
9601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
9661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
9721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
9781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
9841 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
9901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
9961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
10021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
10081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
10141 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
10201 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
10261 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
10321 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
10381 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
10441 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
10501 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 
10561 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 
10621 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 
10681 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 
10741 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 
10801 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 
10861 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 
10921 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 
10981 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 
11041 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 
11101 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 
11161 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 
11221 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 
11281 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 
11341 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 
11401 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 
11461 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 
11521 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 
11581 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 
11641 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 
11701 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 
11761 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 
11821 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 
11881 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 
11941 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 
12001 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 
12061 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt

                            LOCUS dna 12109 bp 
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(4)"
Promoter 2444..3621
/gene="EF1a prom"
ORF 3433..4284
/sequence="ORF_7 rf(5)"
ORF 3670..7101
/sequence="ORF_2 rf(1)"
ORF 4705..5925
/sequence="ORF_6 rf(5)"
misc_binding 7108..7113
/dbxref="REBASE:XbaI"
Promoter 7170..7438
/gene="SV40 prom"
Rep_Origin 7337..7414
/gene="SV40 origin"
ORF 7404..8072
/sequence="ORF_4 rf(3)"
misc_binding 7417..7429
/dbxref="REBASE:SfiI"
Marker 7473..8072
/gene="puro marker"
Other Gene 8682..8734
/gene="delta_U3 other"
Other Gene 8735..8915
/gene="HIV-1_5_LTR other"
Rep_Origin 9421..10040
/gene="pBR322 origin"
Promoter 10693..10742
/gene="NEOKAN prom"
misc_binding 10800..10805
/dbxref="REBASE:BclI"
ORF 10831..11625
/sequence="ORF_3 rf(1)"
Marker 10834..11622
/gene="NTP_II marker"
Rep_Origin 11730..12035
/gene="f1 origin"
BASE COUNT 2644 a 3301 c 3533 g 2631 t 0 others

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

Encap other(162,310)>>> 
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

amp prom(365,393)<<< 
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

HincII 
|
HpaI 
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC

541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC

601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA

HIV-1_5_LTR other(715,895)>>> 
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA

721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA

781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG

841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG

901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG

HIV-1_psi_pack other(1006,1050)>>> 
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT

1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC

NruI 
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT

1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG

1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT

1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG

1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT

1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC

1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT

RRE reg(1557,1790)>>> 
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT

ORF_1 rf(1)(1600,2301)>>> 
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC

1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC

1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG

1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC

1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA

1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG

1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA

1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT

2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT

2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC

2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT

2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT

2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC

ORF_5 rf(4)(2381,3361)<<< 
|
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT

EF1a prom(2444,3621)>>> 
|
2401 aattcaaaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtca 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT

2461 gtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattg 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC

2521 aaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggct 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA

2581 ccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgt 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA

2641 tctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgg 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC

2701 gcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcag 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC

2761 tacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgc 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG

2821 gcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgc 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG

2881 cgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagcc 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG

2941 atttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaat 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA

3001 gcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcc 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG

3061 cgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatc 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG

3121 ggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgt 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA

3181 atcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaaga 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT

3241 tggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagag 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC

3301 cgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttca 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT

3361 tgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttgg 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC

ORF_7 rf(5)(3433,4284)<<< 
|
3421 agtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgag 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC

3481 tgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcc 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG

3541 ctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttttttt 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA

3601 cttccatttcaggtgtcgtgaggaatttcgactgctagcgatatcacaagtttgtacaaa 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCGCTATAGTGTTCAAACATGTTT

ORF_2 rf(1)(3670,7101)>>> 
|
3661 aaagttggcatgccgcgcgctccccgctgccgagccgtgcgctccctgctgcgcagccac 3720
TTTCAACCGTACGGCGCGCGAGGGGCGACGGCTCGGCACGCGAGGGACGACGCGTCGGTG

3721 taccgcgaggtgctgccgctggccacgttcgtgcggcgcctggggccccagggctggcgg 3780
ATGGCGCTCCACGACGGCGACCGGTGCAAGCACGCCGCGGACCCCGGGGTCCCGACCGCC

3781 ctggtgcagcgcggggacccggcggctttccgcgcgctggtggcccagtgcctggtgtgc 3840
GACCACGTCGCGCCCCTGGGCCGCCGAAAGGCGCGCGACCACCGGGTCACGGACCACACG

3841 gtgccctgggacgcacggccgccccccgccgccccctccttccgccaggtgtcctgcctg 3900
CACGGGACCCTGCGTGCCGGCGGGGGGCGGCGGGGGAGGAAGGCGGTCCACAGGACGGAC

3901 aaggagctggtggcccgagtgctgcagaggctgtgcgagcgcggcgcgaagaacgtgctg 3960
TTCCTCGACCACCGGGCTCACGACGTCTCCGACACGCTCGCGCCGCGCTTCTTGCACGAC

3961 gccttcggcttcgcgctgctggacggggcccgcgggggcccccccgaggccttcaccacc 4020
CGGAAGCCGAAGCGCGACGACCTGCCCCGGGCGCCCCCGGGGGGGCTCCGGAAGTGGTGG

4021 agcgtgcgcagctacctgcccaacacggtgaccgacgcactgcgggggagcggggcgtgg 4080
TCGCACGCGTCGATGGACGGGTTGTGCCACTGGCTGCGTGACGCCCCCTCGCCCCGCACC

4081 gggctgctgctgcgccgcgtgggcgacgacgtgctggttcacctgctggcacgctgcgcg 4140
CCCGACGACGACGCGGCGCACCCGCTGCTGCACGACCAAGTGGACGACCGTGCGACGCGC

4141 ctctttgtgctggtggctcccagctgcgcctaccaggtgtgcgggccgccgctgtaccag 4200
GAGAAACACGACCACCGAGGGTCGACGCGGATGGTCCACACGCCCGGCGGCGACATGGTC

4201 ctcggcgctgccactcaggcccggcccccgccacacgctagtggaccccgaaggcgtctg 4260
GAGCCGCGACGGTGAGTCCGGGCCGGGGGCGGTGTGCGATCACCTGGGGCTTCCGCAGAC

4261 ggatgcgaacgggcctggaaccatagcgtcagggaggccggggtccccctgggcctgcca 4320
CCTACGCTTGCCCGGACCTTGGTATCGCAGTCCCTCCGGCCCCAGGGGGACCCGGACGGT

4321 gccccgggtgcgaggaggcgcgggggcagtgccagccgaagtctgccgttgcccaagagg 4380
CGGGGCCCACGCTCCTCCGCGCCCCCGTCACGGTCGGCTTCAGACGGCAACGGGTTCTCC

4381 cccaggcgtggcgctgcccctgagccggagcggacgcccgttgggcaggggtcctgggcc 4440
GGGTCCGCACCGCGACGGGGACTCGGCCTCGCCTGCGGGCAACCCGTCCCCAGGACCCGG

4441 cacccgggcaggacgcgtggaccgagtgaccgtggtttctgtgtggtgtcacctgccaga 4500
GTGGGCCCGTCCTGCGCACCTGGCTCACTGGCACCAAAGACACACCACAGTGGACGGTCT

4501 cccgccgaagaagccacctctttggagggtgcgctctctggcacgcgccactcccaccca 4560
GGGCGGCTTCTTCGGTGGAGAAACCTCCCACGCGAGAGACCGTGCGCGGTGAGGGTGGGT

4561 tccgtgggccgccagcaccacgcgggccccccatccacatcgcggccaccacgtccctgg 4620
AGGCACCCGGCGGTCGTGGTGCGCCCGGGGGGTAGGTGTAGCGCCGGTGGTGCAGGGACC

4621 gacacgccttgtcccccggtgtacgccgagaccaagcacttcctctactcctcaggcgac 4680
CTGTGCGGAACAGGGGGCCACATGCGGCTCTGGTTCGTGAAGGAGATGAGGAGTCCGCTG

ORF_6 rf(5)(4705,5925)<<< 
|
4681 aaggagcagctgcggccctccttcctactcagctctctgaggcccagcctgactggcgct 4740
TTCCTCGTCGACGCCGGGAGGAAGGATGAGTCGAGAGACTCCGGGTCGGACTGACCGCGA

4741 cggaggctcgtggagaccatctttctgggttccaggccctggatgccagggactccccgc 4800
GCCTCCGAGCACCTCTGGTAGAAAGACCCAAGGTCCGGGACCTACGGTCCCTGAGGGGCG

4801 aggttgccccgcctgccccagcgctactggcaaatgcggcccctgtttctggagctgctt 4860
TCCAACGGGGCGGACGGGGTCGCGATGACCGTTTACGCCGGGGACAAAGACCTCGACGAA

4861 gggaaccacgcgcagtgcccctacggggtgctcctcaagacgcactgcccgctgcgagct 4920
CCCTTGGTGCGCGTCACGGGGATGCCCCACGAGGAGTTCTGCGTGACGGGCGACGCTCGA

4921 gcggtcaccccagcagccggtgtctgtgcccgggagaagccccagggctctgtggcggcc 4980
CGCCAGTGGGGTCGTCGGCCACAGACACGGGCCCTCTTCGGGGTCCCGAGACACCGCCGG

4981 cccgaggaggaggacacagacccccgtcgcctggtgcagctgctccgccagcacagcagc 5040
GGGCTCCTCCTCCTGTGTCTGGGGGCAGCGGACCACGTCGACGAGGCGGTCGTGTCGTCG

5041 ccctggcaggtgtacggcttcgtgcgggcctgcctgcgccggctggtgcccccaggcctc 5100
GGGACCGTCCACATGCCGAAGCACGCCCGGACGGACGCGGCCGACCACGGGGGTCCGGAG

5101 tggggctccaggcacaacgaacgccgcttcctcaggaacaccaagaagttcatctccctg 5160
ACCCCGAGGTCCGTGTTGCTTGCGGCGAAGGAGTCCTTGTGGTTCTTCAAGTAGAGGGAC

5161 gggaagcatgccaagctctcgctgcaggagctgacgtggaagatgagcgtgcgggactgc 5220
CCCTTCGTACGGTTCGAGAGCGACGTCCTCGACTGCACCTTCTACTCGCACGCCCTGACG

5221 gcttggctgcgcaggagcccaggggttggctgtgttccggccgcagagcaccgtctgcgt 5280
CGAACCGACGCGTCCTCGGGTCCCCAACCGACACAAGGCCGGCGTCTCGTGGCAGACGCA

5281 gaggagatcctggccaagttcctgcactggctgatgagtgtgtacgtcgtcgagctgctc 5340
CTCCTCTAGGACCGGTTCAAGGACGTGACCGACTACTCACACATGCAGCAGCTCGACGAG

5341 aggtctttcttttatgtcacggagaccacgtttcaaaagaacaggctctttttctaccgg 5400
TCCAGAAAGAAAATACAGTGCCTCTGGTGCAAAGTTTTCTTGTCCGAGAAAAAGATGGCC

5401 aagagtgtctggagcaagttgcaaagcattggaatcagacagcacttgaagagggtgcag 5460
TTCTCACAGACCTCGTTCAACGTTTCGTAACCTTAGTCTGTCGTGAACTTCTCCCACGTC

5461 ctgcgggagctgtcggaagcagaggtcaggcagcatcgggaagccaggcccgccctgctg 5520
GACGCCCTCGACAGCCTTCGTCTCCAGTCCGTCGTAGCCCTTCGGTCCGGGCGGGACGAC

5521 acgtccagactccgcttcatccccaagcctgacgggctgcggccgattgtgaacatggac 5580
TGCAGGTCTGAGGCGAAGTAGGGGTTCGGACTGCCCGACGCCGGCTAACACTTGTACCTG

5581 tacgtcgtgggagccagaacgttccgcagagaaaagagggccgagcgtctcacctcgagg 5640
ATGCAGCACCCTCGGTCTTGCAAGGCGTCTCTTTTCTCCCGGCTCGCAGAGTGGAGCTCC

5641 gtgaaggcactgttcagcgtgctcaactacgagcgggcgcggcgccccggcctcctgggc 5700
CACTTCCGTGACAAGTCGCACGAGTTGATGCTCGCCCGCGCCGCGGGGCCGGAGGACCCG

5701 gcctctgtgctgggcctggacgatatccacagggcctggcgcaccttcgtgctgcgtgtg 5760
CGGAGACACGACCCGGACCTGCTATAGGTGTCCCGGACCGCGTGGAAGCACGACGCACAC

5761 cgggcccaggacccgccgcctgagctgtactttgtcaaggtggatgtgacgggcgcgtac 5820
GCCCGGGTCCTGGGCGGCGGACTCGACATGAAACAGTTCCACCTACACTGCCCGCGCATG

5821 gacaccatcccccaggacaggctcacggaggtcatcgccagcatcatcaaaccccagaac 5880
CTGTGGTAGGGGGTCCTGTCCGAGTGCCTCCAGTAGCGGTCGTAGTAGTTTGGGGTCTTG

5881 acgtactgcgtgcgtcggtatgccgtggtccagaaggccgcccatgggcacgtccgcaag 5940
TGCATGACGCACGCAGCCATACGGCACCAGGTCTTCCGGCGGGTACCCGTGCAGGCGTTC

5941 gccttcaagagccacgtctctaccttgacagacctccagccgtacatgcgacagttcgtg 6000
CGGAAGTTCTCGGTGCAGAGATGGAACTGTCTGGAGGTCGGCATGTACGCTGTCAAGCAC

6001 gctcacctgcaggagaccagcccgctgagggatgccgtcgtcatcgagcagagctcctcc 6060
CGAGTGGACGTCCTCTGGTCGGGCGACTCCCTACGGCAGCAGTAGCTCGTCTCGAGGAGG

6061 ctgaatgaggccagcagtggcctcttcgacgtcttcctacgcttcatgtgccaccacgcc 6120
GACTTACTCCGGTCGTCACCGGAGAAGCTGCAGAAGGATGCGAAGTACACGGTGGTGCGG

6121 gtgcgcatcaggggcaagtcctacgtccagtgccaggggatcccgcagggctccatcctc 6180
CACGCGTAGTCCCCGTTCAGGATGCAGGTCACGGTCCCCTAGGGCGTCCCGAGGTAGGAG

6181 tccacgctgctctgcagcctgtgctacggcgacatggagaacaagctgtttgcggggatt 6240
AGGTGCGACGAGACGTCGGACACGATGCCGCTGTACCTCTTGTTCGACAAACGCCCCTAA

6241 cggcgggacgggctgctcctgcgtttggtggatgatttcttgttggtgacacctcacctc 6300
GCCGCCCTGCCCGACGAGGACGCAAACCACCTACTAAAGAACAACCACTGTGGAGTGGAG

6301 acccacgcgaaaaccttcctcaggaccctggtccgaggtgtccctgagtatggctgcgtg 6360
TGGGTGCGCTTTTGGAAGGAGTCCTGGGACCAGGCTCCACAGGGACTCATACCGACGCAC

6361 gtgaacttgcggaagacagtggtgaacttccctgtagaagacgaggccctgggtggcacg 6420
CACTTGAACGCCTTCTGTCACCACTTGAAGGGACATCTTCTGCTCCGGGACCCACCGTGC

6421 gcttttgttcagatgccggcccacggcctattcccctggtgcggcctgctgctggatacc 6480
CGAAAACAAGTCTACGGCCGGGTGCCGGATAAGGGGACCACGCCGGACGACGACCTATGG

6481 cggaccctggaggtgcagagcgactactccagctatgcccggacctccatcagagccagt 6540
GCCTGGGACCTCCACGTCTCGCTGATGAGGTCGATACGGGCCTGGAGGTAGTCTCGGTCA

6541 ctcaccttcaaccgcggcttcaaggctgggaggaacatgcgtcgcaaactctttggggtc 6600
GAGTGGAAGTTGGCGCCGAAGTTCCGACCCTCCTTGTACGCAGCGTTTGAGAAACCCCAG

6601 ttgcggctgaagtgtcacagcctgtttctggatttgcaggtgaacagcctccagacggtg 6660
AACGCCGACTTCACAGTGTCGGACAAAGACCTAAACGTCCACTTGTCGGAGGTCTGCCAC

6661 tgcaccaacatctacaagatcctcctgctgcaggcgtacaggtttcacgcatgtgtgctg 6720
ACGTGGTTGTAGATGTTCTAGGAGGACGACGTCCGCATGTCCAAAGTGCGTACACACGAC

6721 cagctcccatttcatcagcaagtttggaagaaccccacatttttcctgcgcgtcatctct 6780
GTCGAGGGTAAAGTAGTCGTTCAAACCTTCTTGGGGTGTAAAAAGGACGCGCAGTAGAGA

6781 gacacggcctccctctgctactccatcctgaaagccaagaacgcagggatgtcgctgggg 6840
CTGTGCCGGAGGGAGACGATGAGGTAGGACTTTCGGTTCTTGCGTCCCTACAGCGACCCC

6841 gccaagggcgccgccggccctctgccctccgaggccgtgcagtggctgtgccaccaagca 6900
CGGTTCCCGCGGCGGCCGGGAGACGGGAGGCTCCGGCACGTCACCGACACGGTGGTTCGT

6901 ttcctgctcaagctgactcgacaccgtgtcacctacgtgccactcctggggtcactcagg 6960
AAGGACGAGTTCGACTGAGCTGTGGCACAGTGGATGCACGGTGAGGACCCCAGTGAGTCC

6961 acagcccagacgcagctgagtcggaagctcccggggacgacgctgactgccctggaggcc 7020
TGTCGGGTCTGCGTCGACTCAGCCTTCGAGGGCCCCTGCTGCGACTGACGGGACCTCCGG

7021 gcagccaacccggcactgccctcagacttcaagaccatcctggactgcccaactttcttg 7080
CGTCGGTTGGGCCGTGACGGGAGTCTGAAGTTCTGGTAGGACCTGACGGGTTGAAAGAAC

XbaI 
|
7081 tacaaagtggtggatatctgaggatcctctagactgcagctcgagtacccatacgacgtc 7140
ATGTTTCACCACCTATAGACTCCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAG

SV40 prom(7170,7438)>>> 
|
7141 ccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcagg 7200
GGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCC

7201 cagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccagg 7260
GTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCC

7261 ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtccc 7320
GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGG

SV40 origin(7337,7414)>>> 
|
7321 gcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccca 7380
CGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGT

SfiI 
|
ORF_4 rf(3)(7404,8072)>>> 
| |
7381 tggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctatt 7440
ACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAA

puro marker(7473,8072)>>> 
|
7441 ccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcc 7500
GGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGG

7501 tcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgact 7560
AGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGA

7561 accccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgc 7620
TGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACG

7621 aagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacg 7680
TTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGC

7681 gcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccg 7740
CGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGC

7741 agatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatgg 7800
TCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACC

7801 aagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcg 7860
TTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGC

7861 tctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggagg 7920
AGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCC

7921 cggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccct 7980
GCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGA

7981 tctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgca 8040
AGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGT

8041 cctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggatt 8100
GGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAA

8101 acaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtg 8160
TGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACAC

8161 gatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttct 8220
CTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGA

8221 cctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggc 8280
GGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCG

8281 aacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcca 8340
TTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGT

8341 ccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaac 8400
GGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTG

8401 tcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaatt 8460
AGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAA

8461 ccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacct 8520
GGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGA

8521 ggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttc 8580
CCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAG

8581 cttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagac 8640
GAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTG

delta_U3 other(8682,8734)>>> 
|
8641 gagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactc 8700
CTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAG

HIV-1_5_LTR other(8735,8915)>>> 
|
8701 ccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctg 8760
GGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGAC

8761 agcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcc 8820
TCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGG

8821 ttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct 8880
AACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGA

8881 cagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattat 8940
GTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATA

8941 tcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgc 9000
AGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACG

9001 agcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttt 9060
TCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAA

9061 ttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggca 9120
AAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGT

9121 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 9180
AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT

9181 ttaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaata 9240
AATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTAT

9241 ccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct 9300
GGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA

9301 gcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggga 9360
CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT

9361 taacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc 9420
ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG

pBR322 origin(9421,10040)<<< 
|
9421 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg 9480
GCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGC

9481 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg 9540
GAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACC

9541 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt 9600
TTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAA

9601 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt 9660
AGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCA

9661 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg 9720
CATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGAC

9721 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact 9780
GCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGA

9781 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt 9840
CCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAA

9841 cttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctct 9900
GAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGA

9901 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac 9960
CGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTG

9961 cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc 10020
GCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAG

10021 tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacg 10080
AGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGC

10081 ttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta 10140
AATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAT

10141 aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacca 10200
TTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGT

10201 atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgc 10260
TACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACG

10261 ctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc 10320
GACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACG

10321 tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc 10380
ACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGG

10381 agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctat 10440
TCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATA

10441 taattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgt 10500
ATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACA

10501 tgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgc 10560
ACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACG

10561 tgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaag 10620
ACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTC

10621 agaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatgg 10680
TCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACC

NEOKAN prom(10693,10742)>>> 
|
10681 acagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgc 10740
TGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACG

10741 aaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctct 10800
TTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGA

NTP_II marker(10834,11622)>>> 
|
BclI ORF_3 rf(1)(10831,11625)>>> 
| | |
10801 gatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggt 10860
CTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCA

10861 tctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggc 10920
AGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCG

10921 tgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag 10980
ACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTC

10981 accgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctg 11040
TGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGAC

11041 gccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggac 11100
CGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTG

11101 tggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgcc 11160
ACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGG

11161 gagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacc 11220
CTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGG

11221 tgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagcc 11280
ACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGG

11281 ggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactg 11340
CCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGAC

11341 ttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgat 11400
AAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTA

11401 gcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggc 11460
CGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCG

11461 cggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaa 11520
GCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTT

11521 gagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgat 11580
CTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTA

11581 tcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaattt 11640
AGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAA

11641 ttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatc 11700
AACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAG

f1 origin(11730,12035)<<< 
|
11701 aaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactatt 11760
TTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAA

11761 aaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccact 11820
TTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGA

11821 acgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcg 11880
TGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGC

11881 gaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgag 11940
CTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTC

11941 aaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcac 12000
TTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTG

12001 gctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgc 12060
CGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCG

12061 cattcaggatcgaattaattcttaattaacatcatcaataatatacctt 12109
GTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA