pLenti-EF1a-dCas9-tet1CD
| Vector Name | pLenti-EF1a-dCas9-tet1CD |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | EF1a forward sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' |
| Additional Information | This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities; whereas the TET1 domain facilitates the process of demethylation leading to transcriptional up-regulation. By using specific sgRNAs, the dCas9-TET1CD can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagca ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtcc aaagaagaag cggaaggtcc
7861 tgcccacctg cagctgtctt gatcgagtta tacaaaaaga caaaggccca tattatacac
7921 accttggggc aggaccaagt gttgctgctg tcagggaaat catggagaat aggtatggtc
7981 aaaaaggaaa cgcaataagg atagaaatag tagtgtacac cggtaaagaa gggaaaagct
8041 ctcatgggtg tccaattgct aagtgggttt taagaagaag cagtgatgaa gaaaaagttc
8101 tttgtttggt ccggcagcgt acaggccacc actgtccaac tgctgtgatg gtggtgctca
8161 tcatggtgtg ggatggcatc cctcttccaa tggccgaccg gctatacaca gagctcacag
8221 agaatctaaa gtcatacaat gggcacccta ccgacagaag atgcaccctc aatgaaaatc
8281 gtacctgtac atgtcaagga attgatccag agacttgtgg agcttcattc tcttttggct
8341 gttcatggag tatgtacttt aatggctgta agtttggtag aagcccaagc cccagaagat
8401 ttagaattga tccaagctct cccttacatg aaaaaaacct tgaagataac ttacagagtt
8461 tggctacacg attagctcca atttataagc agtatgctcc agtagcttac caaaatcagg
8521 tggaatatga aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt cgacccttct
8581 ctggggtcac tgcttgcctg gacttctgtg ctcatcccca cagggacatt cacaacatga
8641 ataatggaag cactgtggtt tgtaccttaa ctcgagaaga taaccgctct ttgggtgtta
8701 ttcctcaaga tgagcagctc catgtgctac ctctttataa gctttcagac acagatgagt
8761 ttggctccaa ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag gtcctggcac
8821 cccgccgcaa aaaaagaacg tgtttcactc agcctgttcc ccgttctgga aagaagaggg
8881 ctgcgatgat gacagaggtt cttgcacata agataagggc agtggaaaag aaacctattc
8941 cccgaatcaa gcggaagaat aactcaacaa caacaaacaa cagtaagcct tcgtcactgc
9001 caaccttagg gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa accgaacccc
9061 attttatctt aaaaagttca gacaacacta aaacttattc gctgatgcca tccgctcctc
9121 acccagtgaa agaggcatct ccaggcttct cctggtcccc gaagactgct tcagccacac
9181 cagctccact gaagaatgac gcaacagcct catgcgggtt ttcagaaaga agcagcactc
9241 cccactgtac gatgccttcg ggaagactca gtggtgccaa tgctgcagct gctgatggcc
9301 ctggcatttc acagcttggc gaagtggctc ctctccccac cctgtctgct cctgtgatgg
9361 agcccctcat taattctgag ccttccactg gtgtgactga gccgctaacg cctcatcagc
9421 caaaccacca gccctccttc ctcacctctc ctcaagacct tgcctcttct ccaatggaag
9481 aagatgagca gcattctgaa gcagatgagc ctccatcaga cgaaccccta tctgatgacc
9541 ccctgtcacc tgctgaggag aaattgcccc acattgatga gtattggtca gacagtgagc
9601 acatcttttt ggatgcaaat attggtgggg tggccatcgc acctgctcac ggctcggttt
9661 tgattgagtg tgcccggcga gagctgcacg ctaccactcc tgttgagcac cccaaccgta
9721 atcatccaac ccgcctctcc cttgtctttt accagcacaa aaacctaaat aagccccaac
9781 atggttttga actaaacaag attaagtttg aggctaaaga agctaagaat aagaaaatga
9841 aggcctcaga gcaaaaagac caggcagcta atgaaggtcc agaacagtcc tctgaagtaa
9901 atgaattgaa ccaaattcct tctcataaag cattaacatt aacccatgac aatgttgtca
9961 ccgtgtcccc ttatgctctc acacacgttg cggggcccta taaccattgg gtcactagtc
10021 caaaaaagaa aagaaaggtg tgagtttaaa cacgcgtggt gtggaaagtc cccaggctcc
10081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
10141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
10201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
10261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
10321 gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt acaagcccac
10381 ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg ccgccgcgtt
10441 cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg agcgggtcac
10501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
10561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
10621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
10681 acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
10741 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
10801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
10861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
10921 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg gaaatcaacc
10981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
11041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
11101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
11161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
11221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
11281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
11341 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
11401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
11461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tcgccttcgc
11521 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat ggaagggcta
11581 attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct ctggttagac
11641 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
11701 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
11761 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
11821 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
11881 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
11941 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
12001 gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg
12061 ggtctgcatt aatgaatcgg ccaacgatcc cggtgtgaaa taccgcacag atgcgtaagg
12121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
12181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
12241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
12301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
12361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
12421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
12481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
12541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
12601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
12661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
12721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
12781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
12841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
12901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
12961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
13021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
13081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
13141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
13201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
13261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
13321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
13381 aacgttgttg aaaaaggatc ttcacctaga tccttttcac gtagaaagcc agtccgcaga
13441 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
13501 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
13561 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
13621 agccctgcaa agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat
13681 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
13741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
13801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
13861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
13921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
13981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
14041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
14101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
14161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
14221 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
14281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
14341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
14401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
14461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattttgt
14521 taaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caacatccct
14581 tataaatcaa aagaatagac cgcgataggg ttgagtgttg ttccagtttg gaacaagagt
14641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
14701 ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
14761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
14821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
14881 gcggtcacgc tgcgcgtaac caccacaccc gcgcgcttaa tgcgccgcta cagggcgcgt
14941 ccattcgcca ttcaggatcg aattaattct taattaacat catcaataat atacctt
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagca ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtcc aaagaagaag cggaaggtcc
7861 tgcccacctg cagctgtctt gatcgagtta tacaaaaaga caaaggccca tattatacac
7921 accttggggc aggaccaagt gttgctgctg tcagggaaat catggagaat aggtatggtc
7981 aaaaaggaaa cgcaataagg atagaaatag tagtgtacac cggtaaagaa gggaaaagct
8041 ctcatgggtg tccaattgct aagtgggttt taagaagaag cagtgatgaa gaaaaagttc
8101 tttgtttggt ccggcagcgt acaggccacc actgtccaac tgctgtgatg gtggtgctca
8161 tcatggtgtg ggatggcatc cctcttccaa tggccgaccg gctatacaca gagctcacag
8221 agaatctaaa gtcatacaat gggcacccta ccgacagaag atgcaccctc aatgaaaatc
8281 gtacctgtac atgtcaagga attgatccag agacttgtgg agcttcattc tcttttggct
8341 gttcatggag tatgtacttt aatggctgta agtttggtag aagcccaagc cccagaagat
8401 ttagaattga tccaagctct cccttacatg aaaaaaacct tgaagataac ttacagagtt
8461 tggctacacg attagctcca atttataagc agtatgctcc agtagcttac caaaatcagg
8521 tggaatatga aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt cgacccttct
8581 ctggggtcac tgcttgcctg gacttctgtg ctcatcccca cagggacatt cacaacatga
8641 ataatggaag cactgtggtt tgtaccttaa ctcgagaaga taaccgctct ttgggtgtta
8701 ttcctcaaga tgagcagctc catgtgctac ctctttataa gctttcagac acagatgagt
8761 ttggctccaa ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag gtcctggcac
8821 cccgccgcaa aaaaagaacg tgtttcactc agcctgttcc ccgttctgga aagaagaggg
8881 ctgcgatgat gacagaggtt cttgcacata agataagggc agtggaaaag aaacctattc
8941 cccgaatcaa gcggaagaat aactcaacaa caacaaacaa cagtaagcct tcgtcactgc
9001 caaccttagg gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa accgaacccc
9061 attttatctt aaaaagttca gacaacacta aaacttattc gctgatgcca tccgctcctc
9121 acccagtgaa agaggcatct ccaggcttct cctggtcccc gaagactgct tcagccacac
9181 cagctccact gaagaatgac gcaacagcct catgcgggtt ttcagaaaga agcagcactc
9241 cccactgtac gatgccttcg ggaagactca gtggtgccaa tgctgcagct gctgatggcc
9301 ctggcatttc acagcttggc gaagtggctc ctctccccac cctgtctgct cctgtgatgg
9361 agcccctcat taattctgag ccttccactg gtgtgactga gccgctaacg cctcatcagc
9421 caaaccacca gccctccttc ctcacctctc ctcaagacct tgcctcttct ccaatggaag
9481 aagatgagca gcattctgaa gcagatgagc ctccatcaga cgaaccccta tctgatgacc
9541 ccctgtcacc tgctgaggag aaattgcccc acattgatga gtattggtca gacagtgagc
9601 acatcttttt ggatgcaaat attggtgggg tggccatcgc acctgctcac ggctcggttt
9661 tgattgagtg tgcccggcga gagctgcacg ctaccactcc tgttgagcac cccaaccgta
9721 atcatccaac ccgcctctcc cttgtctttt accagcacaa aaacctaaat aagccccaac
9781 atggttttga actaaacaag attaagtttg aggctaaaga agctaagaat aagaaaatga
9841 aggcctcaga gcaaaaagac caggcagcta atgaaggtcc agaacagtcc tctgaagtaa
9901 atgaattgaa ccaaattcct tctcataaag cattaacatt aacccatgac aatgttgtca
9961 ccgtgtcccc ttatgctctc acacacgttg cggggcccta taaccattgg gtcactagtc
10021 caaaaaagaa aagaaaggtg tgagtttaaa cacgcgtggt gtggaaagtc cccaggctcc
10081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
10141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
10201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
10261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
10321 gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt acaagcccac
10381 ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg ccgccgcgtt
10441 cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg agcgggtcac
10501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
10561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
10621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
10681 acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
10741 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
10801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
10861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
10921 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg gaaatcaacc
10981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
11041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
11101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
11161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
11221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
11281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
11341 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
11401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
11461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tcgccttcgc
11521 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat ggaagggcta
11581 attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct ctggttagac
11641 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
11701 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
11761 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
11821 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
11881 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
11941 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
12001 gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg
12061 ggtctgcatt aatgaatcgg ccaacgatcc cggtgtgaaa taccgcacag atgcgtaagg
12121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
12181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
12241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
12301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
12361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
12421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
12481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
12541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
12601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
12661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
12721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
12781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
12841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
12901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
12961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
13021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
13081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
13141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
13201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
13261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
13321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
13381 aacgttgttg aaaaaggatc ttcacctaga tccttttcac gtagaaagcc agtccgcaga
13441 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
13501 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
13561 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
13621 agccctgcaa agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat
13681 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
13741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
13801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
13861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
13921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
13981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
14041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
14101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
14161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
14221 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
14281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
14341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
14401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
14461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattttgt
14521 taaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caacatccct
14581 tataaatcaa aagaatagac cgcgataggg ttgagtgttg ttccagtttg gaacaagagt
14641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
14701 ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
14761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
14821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
14881 gcggtcacgc tgcgcgtaac caccacaccc gcgcgcttaa tgcgccgcta cagggcgcgt
14941 ccattcgcca ttcaggatcg aattaattct taattaacat catcaataat atacctt