pLenti-EF1a-dCas9-SV40-Puro
| Vector Name | pLenti-EF1a-dCas9-SV40-Puro |
| Antibiotic Information | Bacterial Selection: Kanamycin Eukaryotic Selection: Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagaa ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
7861 cggccgctgg atcctctaga ctgcagctcg agtacccata cgacgtccca gactacgctt
7921 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
7981 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
8041 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
8101 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
8161 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
8221 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
8281 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
8341 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
8401 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
8461 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
8521 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc
8581 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
8641 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
8701 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
8761 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
8821 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga
8881 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
8941 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
9001 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
9061 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
9121 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
9181 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
9241 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
9301 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
9361 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc
9421 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa
9481 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
9541 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
9601 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
9661 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
9721 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
9781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
9841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg
9901 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc
9961 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
10021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
10081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
10141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
10201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
10261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
10321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
10381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
10441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
10501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
10561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
10621 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
10681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
10741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
10801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
10861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
10921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
10981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
11041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
11101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
11161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
11221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct
11281 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga
11341 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag
11401 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac
11461 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
11521 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag
11581 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
11641 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
11701 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
11761 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
11821 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
11881 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
11941 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
12001 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
12061 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
12121 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
12181 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
12241 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
12301 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
12361 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc
12421 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc
12481 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
12541 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
12601 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg
12661 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
12721 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
12781 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga
12841 attaattctt aattaacatc atcaataata tacctt
LOCUS dna 12876 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(6)"
Promoter 2444..3621
/gene="EF1a prom"
misc_binding 3056..3061
/dbxref="REBASE:ApaI"
ORF 3675..7922
/sequence="ORF_3 rf(3)"
misc_binding 4152..4157
/dbxref="REBASE:NdeI"
misc_binding 5041..5046
/dbxref="REBASE:XmaI"
misc_binding 5041..5046
/dbxref="REBASE:SmaI"
misc_binding 7497..7502
/dbxref="REBASE:EcoRI"
misc_binding 7860..7867
/dbxref="REBASE:NotI"
misc_binding 7875..7880
/dbxref="REBASE:XbaI"
Promoter 7937..8205
/gene="SV40 prom"
Rep_Origin 8104..8181
/gene="SV40 origin"
ORF 8171..8839
/sequence="ORF_2 rf(2)"
misc_binding 8184..8196
/dbxref="REBASE:SfiI"
Marker 8240..8839
/gene="puro marker"
Other Gene 9449..9501
/gene="delta_U3 other"
Other Gene 9502..9682
/gene="HIV-1_5_LTR other"
Rep_Origin 10188..10807
/gene="pBR322 origin"
Promoter 11460..11509
/gene="NEOKAN prom"
ORF 11598..12392
/sequence="ORF_4 rf(3)"
Marker 11601..12389
/gene="NTP_II marker"
Rep_Origin 12497..12802
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
ORF_5 rf(6)(2381,3361)<<<
|
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
EF1a prom(2444,3621)>>>
|
2401 aattcaaaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtca 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT
2461 gtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattg 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC
2521 aaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggct 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA
2581 ccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgt 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA
2641 tctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgg 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC
2701 gcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcag 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC
2761 tacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgc 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG
2821 gcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgc 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG
2881 cgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagcc 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG
2941 atttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaat 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA
3001 gcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcc 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG
ApaI
|
3061 cgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatc 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG
3121 ggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgt 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA
3181 atcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaaga 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT
3241 tggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagag 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC
3301 cgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttca 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT
3361 tgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttgg 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC
3421 agtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgag 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC
3481 tgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcc 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG
3541 ctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttttttt 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA
3601 cttccatttcaggtgtcgtgaggaatttcgactgctagaaataattttgtttaactttaa 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCTTTATTAAAACAAATTGAAATT
ORF_3 rf(3)(3675,7922)>>>
|
3661 gaaggagatataccatggacaagaagtactccattgggctcgcgatcggcacaaacagcg 3720
CTTCCTCTATATGGTACCTGTTCTTCATGAGGTAACCCGAGCGCTAGCCGTGTTTGTCGC
3721 tcggctgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgg 3780
AGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACC
3781 gcaataccgatcgccacagcataaagaagaacctcattggcgccctcctgttcgactccg 3840
CGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGC
3841 gggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaa 3900
CCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTT
3901 agaatcggatctgctacctgcaggagatctttagtaatgagatggctaaggtggatgact 3960
TCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGA
3961 ctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcacgagcgcc 4020
GAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGG
4021 acccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatat 4080
TGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATA
4081 atcatctgaggaagaagcttgtagacagtactgataaggctgacttgcggttgatctatc 4140
TAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAG
NdeI
|
4141 tcgcgctggcgcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacc 4200
AGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGG
4201 cagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttt 4260
GTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAA
4261 tcgaagagaacccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggc 4320
AGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCG
4321 tgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacg 4380
ACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGC
4381 gcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaact 4440
CGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGA
4441 tcgacctggccgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcg 4500
AGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGC
4501 acaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacc 4560
TGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGG
4561 tgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctc 4620
ACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAG
4621 cgctgagcgctagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctga 4680
GCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACT
4681 aggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtcta 4740
TCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGAT
4741 aaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaattta 4800
TTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAAT
4801 ttaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagag 4860
AATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTC
4861 aagatctgttgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacc 4920
TTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGG
4921 tgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagata 4980
ACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTAT
4981 acagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcg 5040
TGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGC
SmaI
|
XmaI
| |
5041 cccggggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccct 5100
GGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGA
5101 ggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatga 5160
CCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACT
5161 ctaactttgataaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacg 5220
GATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGC
5221 agtacttcacagtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaa 5280
TCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTT
5281 agccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacga 5340
TCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCT
5341 accggaaagttaccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcg 5400
TGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGC
5401 actctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacg 5460
TGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGC
5461 atctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattc 5520
TAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAG
5521 ttgaggacattgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgct 5580
AACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGA
5581 tgaaaacttacgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgat 5640
ACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTA
5641 atacaggatgggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtg 5700
TATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCAC
5701 gaaagacaatcctggattttcttaagtccgatggatttgccaaccggaacttcatgcagt 5760
CTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCA
5761 tgatccatgatgactctctcacctttaaggaggacatccagaaagcacaagtttctggcc 5820
ACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGG
5821 agggggacagtcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagg 5880
TCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCC
5881 gaatactgcagaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagc 5940
CTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCG
5941 ccgagaatatcgttatcgagatggcccgagagaaccaaactacccagaagggacagaaga 6000
GGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCT
6001 acagtagggaaaggatgaagaggattgaagagggtataaaagaactggggtcccaaatcc 6060
TGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGG
6061 ttaaggaacacccagttgaaaacacccagcttcagaatgagaagctctacctgtactacc 6120
AATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGG
6121 tgcagaacggcagggacatgtacgtggatcaggaactggacatcaatcggctctccgact 6180
ACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGA
6181 acgacgtggatgcgatcgtgccccagtcttttctcaaagatgattctattgataataaag 6240
TGCTGCACCTACGCTAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTC
6241 tgttgacaagatccgataaaaatagagggaagagtgataacgtcccctcagaagaagttg 6300
ACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAAC
6301 tcaagaaaatgaaaaattattggcggcagctgctgaacgccaaactgatcacacaacgga 6360
AGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCT
6361 agttcgataatctgactaaggctgaacgaggtggcctgtctgagttggataaagccggct 6420
TCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGA
6421 tcatcaaaaggcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcg 6480
AGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGC
6481 attcacgcatgaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagtta 6540
TAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAAT
6541 ttactctgaagtctaagctggtctcagatttcagaaaggactttcagttttataaggtga 6600
AATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACT
6601 gagagatcaacaattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactg 6660
CTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGAC
6661 cacttatcaaaaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgt 6720
GTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACA
6721 acgatgttaggaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagt 6780
TGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCA
6781 acttcttttacagcaatattatgaattttttcaagaccgagattacactggccaatggag 6840
TGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTC
6841 agattcggaagcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggaca 6900
TCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGT
6901 agggtagggatttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgtta 6960
TCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAAT
6961 aaaagaccgaagtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaaca 7020
TTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGT
7021 gcgacaagctgatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgatt 7080
CGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAA
7081 ctcctacagtcgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaa 7140
GAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTT
7141 aactcaaaagcgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaa 7200
TTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTT
7201 aaaaccccatcgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatca 7260
TTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGT
7261 ttaagcttcccaagtactctctctttgagcttgaaaacggccggaaacgaatgctcgcta 7320
AATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGAT
7321 gtgcgggcgagctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttct 7380
CACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGA
7381 tgtatctggccagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagc 7440
ACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCG
EcoRI
|
7441 agctgttcgtggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgaat 7500
TCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTA
7501 tctccaaaagagtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaata 7560
AGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTAT
7561 agcacagggataagcccatcagggagcaggcagaaaacattatccacttgtttactctga 7620
TCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACT
7621 ccaacttgggcgcgcctgcagccttcaagtacttcgacaccaccatagacagaaagcggt 7680
GGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCA
7681 acacctctacaaaggaggtcctggacgccacactgattcatcagtcaattacggggctct 7740
TGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGA
7741 atgaaacaagaatcgacctctctcagctcggtggagacagcagggctgaccccaagaaga 7800
TACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCT
7801 agaggaaggtggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccg 7860
TCTCCTTCCACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGC
NotI XbaI
| |
7861 cggccgctggatcctctagactgcagctcgagtacccatacgacgtcccagactacgctt 7920
GCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAA
SV40 prom(7937,8205)>>>
|
7921 gagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa 7980
CTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTT
7981 agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggc 8040
TCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCG
8041 agaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccg 8100
TCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGC
SV40 origin(8104,8181)>>>
|
8101 cccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatt 8160
GGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAA
SfiI
|
ORF_2 rf(2)(8171,8839)>>>
| |
8161 ttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtga 8220
AAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACT
puro marker(8240,8839)>>>
|
8221 ggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcga 8280
CCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCT
8281 cgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcg 8340
GCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGC
8341 ccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcct 8400
GGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGA
8401 cacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggc 8460
GTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCG
8461 ggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcg 8520
CCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGC
8521 catggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggc 8580
GTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCG
8581 gccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgacca 8640
CGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGT
8641 ccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgc 8700
GGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCG
8701 cggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggct 8760
GCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGA
8761 cggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgac 8820
GCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTG
8821 ccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtga 8880
GGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACT
8881 aagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgcttt 8940
TTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAA
8941 aatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataa 9000
TTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATT
9001 atcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggt 9060
TAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCA
9061 gtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagct 9120
CACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGA
9121 cctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctg 9180
GGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGAC
9181 ccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtc 9240
GGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAG
9241 ggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgg 9300
CCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCC
9301 gacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcct 9360
CTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGA
9361 gctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcc 9420
CGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGG
delta_U3 other(9449,9501)>>>
|
9421 ctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaa 9480
GAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTT
HIV-1_5_LTR other(9502,9682)>>>
|
9481 gatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctc 9540
CTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAG
9541 tctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaa 9600
AGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTT
9601 gtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttag 9660
CATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATC
9661 tcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataa 9720
AGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATT
9721 cttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggt 9780
GAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCA
9781 tacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattct 9840
ATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGA
9841 agttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtg 9900
TCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCAC
9901 cggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggc 9960
GCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCG
9961 caacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgc 10020
GTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCG
10021 tcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta 10080
AGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCAT
10081 tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaag 10140
AGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTC
pBR322 origin(10188,10807)<<<
|
10141 aacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcg 10200
TTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGC
10201 tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg 10260
AAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCC
10261 tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtg 10320
ACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCAC
10321 cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga 10380
GCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCT
10381 agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgc 10440
TCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCG
10441 tccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggt 10500
AGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCA
10501 aactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact 10560
TTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGA
10561 ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgg 10620
CCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACC
10621 cctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagtt 10680
GGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAA
10681 accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggt 10740
TGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCA
10741 ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct 10800
CCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGA
10801 ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg 10860
AACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAAC
10861 gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt 10920
CAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAA
10921 aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt 10980
TTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCA
10981 gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc 11040
CTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAG
11041 gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccg 11100
CACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGC
11101 cgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggcc 11160
GCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGG
11161 gagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg 11220
CTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCC
11221 gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatct 11280
CTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGA
11281 tcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatga 11340
AGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACT
11341 atgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtag 11400
TACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATC
11401 cttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaac 11460
GAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTG
NEOKAN prom(11460,11509)>>>
|
11461 cggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactgga 11520
GCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCT
11521 tggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacag 11580
ACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTC
NTP_II marker(11601,12389)>>>
|
ORF_4 rf(3)(11598,12392)>>>
| |
11581 gatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgctt 11640
CTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAA
11641 gggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccg 11700
CCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGC
11701 ccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccg 11760
GGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGC
11761 gtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcg 11820
CACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGC
11821 ttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgg 11880
AAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACC
11881 gcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcca 11940
CGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGT
11941 tcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgacc 12000
AGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGG
12001 accaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 12060
TGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAG
12061 aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctca 12120
TCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGT
12121 aggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccga 12180
TCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCT
12181 atatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtgg 12240
TATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACC
12241 cggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcg 12300
GCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGC
12301 aatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcg 12360
TTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGC
12361 ccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagc 12420
GGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCG
12421 tcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagacc 12480
AGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGG
f1 origin(12497,12802)<<<
|
12481 gcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggac 12540
CGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTG
12541 tccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatca 12600
AGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGT
12601 cccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaaggg 12660
GGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCC
12661 agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaag 12720
TCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTC
12721 aaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacc 12780
TTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGG
12781 accacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcga 12840
TGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCT
12841 attaattcttaattaacatcatcaataatatacctt 12876
TAATTAAGAATTAATTGTAGTAGTTATTATATGGAA