pLenti-EF1a-dCas9-SAM
| Vector Name | pLenti-EF1a-dCas9-SAM |
| Antibiotic Information | Bacterial Selection: Kanamycin Eukaryotic Selection: Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagca ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
7861 cggccgcttc gccagggatc cgtcgacttg acgcgttgat atcaacaagt ttgtacaaaa
7921 aagcaggcta caaagaggcc agcggttccg gacgggctga cgcattggac gattttgatc
7981 tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg
8041 cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg
8101 acatgctgat taactcaaga agttccggat ctccgaaaaa gaaacgcaaa gttggtagcc
8161 agtacctgcc cgacaccgac gaccggcacc ggatcgagga aaagcggaag cggacctacg
8221 agacattcaa gagcatcatg aagaagtccc ccttcagcgg ccccaccgac cctagacctc
8281 cacctagaag aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa cctgcccccc
8341 agccttaccc cttcaccagc agcctgagca ccatcaacta cgacgagttc cctaccatgg
8401 tgttccccag cggccagatc tctcaggcct ctgctctggc tccagcccct cctcaggtgc
8461 tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg gctcaggcac
8521 cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca ccagccccta
8581 aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg cagttcgacg
8641 acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc accgacctgg
8701 ccagcgtgga caacagcgag ttccaacagc tgctgaacca gggcatccct gtggcccctc
8761 acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc gtgacaggcg
8821 ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg cctaatggac
8881 tgctgtctgg cgacgaggac ttcagctcta tcgccgatat ggatttctca gccttgctgg
8941 gctctggcag cggcagccgg gattccaggg aagggatgtt tttgccgaag cctgaggccg
9001 gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg ccagccaaaa cgaatccggc
9061 catttcatcc tccaggaagt ccatgggcca accgcccact ccccgccagc ctcgcaccaa
9121 caccaaccgg tccagtacat gagccagtcg ggtcactgac cccggcacca gtccctcagc
9181 cactggatcc agcgcccgca gtgactcccg aggccagtca cctgttggag gatcccgatg
9241 aagagacgag ccaggctgtc aaagcccttc gggagatggc cgatactgtg attccccaga
9301 aggaagaggc tgcaatctgt ggccaaatgg acctttccca tccgccccca aggggccatc
9361 tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg gactcacccc
9421 tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc ctcttgcatg
9481 ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgtttact agtccaaaaa
9541 agaaaagaaa ggtgtgagtt taaacacgcg tggtgtggaa agtccccagg ctccccagca
9601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
9661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
9721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
9781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
9841 ttccagaagt agtgaggagg cttttttgga ggccatgacc gagtacaagc ccacggtgcg
9901 cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc ctcgccgccg cgttcgccga
9961 ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac atcgagcggg tcaccgagct
10021 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
10081 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
10141 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
10201 ggaagggctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
10261 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
10321 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
10381 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
10441 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt tccggaaatc aacctctgga
10501 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
10561 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
10621 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
10681 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
10741 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
10801 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
10861 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
10921 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
10981 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag
11041 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac
11101 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
11161 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
11221 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
11281 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
11341 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
11401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
11461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
11521 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg
11581 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa
11641 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
11701 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
11761 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
11821 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
11881 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
11941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
12001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
12061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
12121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
12181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
12241 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
12301 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
12361 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
12421 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
12481 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
12541 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
12601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
12661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
12721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
12781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
12841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
12901 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
12961 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
13021 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
13081 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
13141 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct
13201 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
13261 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
13321 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
13381 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
13441 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
13501 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
13561 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
13621 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
13681 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
13741 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
13801 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
13861 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
13921 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
13981 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat
14041 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa
14101 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta
14161 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
14221 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat
14281 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
14341 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
14401 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc
14461 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t
LOCUS dna 14511 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(6)"
Promoter 2444..3621
/gene="EF1a prom"
misc_binding 3056..3061
/dbxref="REBASE:ApaI"
ORF 3675..9557
/sequence="ORF_3 rf(3)"
misc_binding 4152..4157
/dbxref="REBASE:NdeI"
misc_binding 5041..5046
/dbxref="REBASE:XmaI"
misc_binding 5041..5046
/dbxref="REBASE:SmaI"
misc_binding 7497..7502
/dbxref="REBASE:EcoRI"
misc_binding 7860..7867
/dbxref="REBASE:NotI"
misc_binding 7898..7903
/dbxref="REBASE:EcoRV"
Other Gene 7905..7929
/gene="attB1 other"
Promoter 9572..9840
/gene="SV40 prom"
Rep_Origin 9739..9816
/gene="SV40 origin"
ORF 9806..10474
/sequence="ORF_2 rf(2)"
misc_binding 9819..9831
/dbxref="REBASE:SfiI"
Marker 9875..10474
/gene="puro marker"
Other Gene 11084..11136
/gene="delta_U3 other"
Other Gene 11137..11317
/gene="HIV-1_5_LTR other"
Rep_Origin 11823..12442
/gene="pBR322 origin"
Promoter 13095..13144
/gene="NEOKAN prom"
ORF 13233..14027
/sequence="ORF_4 rf(3)"
Marker 13236..14024
/gene="NTP_II marker"
Rep_Origin 14132..14437
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 TTTTGGATTGAAGCCAATATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGT 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 GGGAACGGGGCGGGTGACGTAGTAGTGTGGCGGAAGTGTGATGTTGCAAGTGTGGCGGAA 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 CACATGTAAGCGACGGATGTGGCAAAAGTGACGTTTTTGGTGTGCGCCGGTGTACACAGG 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 AAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTAGTAAATTTGGGCGTAACCGAGT 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 AAGATTTGGCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTGAAATCTGAATAATTTTG 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 TGTTACTCATAGCGCGTAATACGGCAGACCTCAGCGCTAGATTATTGAAGCATTTATCAG 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTAACTATAACGGTCCTAAGGTA 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 GCGAAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAG 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 CAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGG 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 TACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACT 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 GAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCT 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 CTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTT 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 AAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGAC 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 TCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGC 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 GCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTC 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 GGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAA 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 TTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGG 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 GGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATA 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 AATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCC 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 TGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGA 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 CAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATC 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 AAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACA 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 AAAGTAAGACCACCGCACAGCAAGCCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAG 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 GGACATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTA 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGA 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 GCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACG 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTG 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 AGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTC 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 CAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGG 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 GGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 AAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAAC 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 AATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAAT 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 GAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACA 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 AATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 ATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCG 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 TTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAA 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 GGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGA 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 AAGCTTGGGATTCGAATTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAG 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
ORF_5 rf(6)(2381,3361)<<<
|
2341 AATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACAAAAACAAATTACAAA 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
EF1a prom(2444,3621)>>>
|
2401 AATTCAAAATTTTCGGGTTTTTCGAACCTAGGGAGTGGGAATTGGCTCCGGTGCCCGTCA 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT
2461 GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTG 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC
2521 AACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCT 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA
2581 CCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGT 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA
2641 TCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGG 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC
2701 GCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAG 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC
2761 TACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGC 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG
2821 GCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGC 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG
2881 CGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCC 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG
2941 ATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAAT 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA
3001 GCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCC 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG
ApaI
|
3061 CGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATC 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG
3121 GGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGT 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA
3181 ATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGA 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT
3241 TGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAG 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC
3301 CGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCA 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT
3361 TGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGG 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC
3421 AGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAG 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC
3481 TGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCC 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG
3541 CTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTT 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA
3601 CTTCCATTTCAGGTGTCGTGAGGAATTTCGACTGCTAGCAATAATTTTGTTTAACTTTAA 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCGTTATTAAAACAAATTGAAATT
ORF_3 rf(3)(3675,9557)>>>
|
3661 GAAGGAGATATACCATGGACAAGAAGTACTCCATTGGGCTCGCGATCGGCACAAACAGCG 3720
CTTCCTCTATATGGTACCTGTTCTTCATGAGGTAACCCGAGCGCTAGCCGTGTTTGTCGC
3721 TCGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGG 3780
AGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACC
3781 GCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCG 3840
CGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGC
3841 GGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAA 3900
CCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTT
3901 AGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACT 3960
TCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGA
3961 CTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCC 4020
GAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGG
4021 ACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATAT 4080
TGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATA
4081 ATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATC 4140
TAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAG
NdeI
|
4141 TCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACC 4200
AGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGG
4201 CAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTT 4260
GTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAA
4261 TCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGC 4320
AGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCG
4321 TGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACG 4380
ACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGC
4381 GCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCTAACT 4440
CGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGA
4441 TCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCG 4500
AGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGC
4501 ACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACC 4560
TGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGG
4561 TGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTC 4620
ACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAG
4621 CGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGA 4680
GCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACT
4681 AGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTA 4740
TCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGAT
4741 AAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTA 4800
TTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAAT
4801 TTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAG 4860
AATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTC
4861 AAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACC 4920
TTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGG
4921 TGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATA 4980
ACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTAT
4981 ACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCG 5040
TGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGC
SmaI
|
XmaI
| |
5041 CCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCT 5100
GGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGA
5101 GGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGA 5160
CCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACT
5161 CTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACG 5220
GATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGC
5221 AGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAA 5280
TCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTT
5281 AGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGA 5340
TCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCT
5341 ACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCG 5400
TGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGC
5401 ACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACG 5460
TGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGC
5461 ATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTC 5520
TAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAG
5521 TTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAACGCT 5580
AACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGA
5581 TGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGAT 5640
ACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTA
5641 ATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAGCAGAGTG 5700
TATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCAC
5701 GAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGT 5760
CTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCA
5761 TGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCC 5820
ACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGG
5821 AGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGG 5880
TCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCC
5881 GAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCATAAGC 5940
CTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCG
5941 CCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGA 6000
GGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCT
6001 ACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCC 6060
TGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGG
6061 TTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACC 6120
AATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGG
6121 TGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACT 6180
ACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGA
6181 ACGACGTGGATGCGATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAG 6240
TGCTGCACCTACGCTAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTC
6241 TGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTG 6300
ACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAAC
6301 TCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGA 6360
AGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCT
6361 AGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCT 6420
TCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGA
6421 TCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCG 6480
AGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGC
6481 ATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTA 6540
TAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAAT
6541 TTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGA 6600
AATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACT
6601 GAGAGATCAACAATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTG 6660
CTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGAC
6661 CACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGT 6720
GTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACA
6721 ACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGT 6780
TGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCA
6781 ACTTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAG 6840
TGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTC
6841 AGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACA 6900
TCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGT
6901 AGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTA 6960
TCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAAT
6961 AAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACA 7020
TTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGT
7021 GCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATT 7080
CGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAA
7081 CTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAA 7140
GAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTT
7141 AACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAA 7200
TTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTT
7201 AAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCA 7260
TTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGT
7261 TTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCGCTA 7320
AATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGAT
7321 GTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCT 7380
CACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGA
7381 TGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGC 7440
ACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCG
EcoRI
|
7441 AGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAATAAGCGAAT 7500
TCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTA
7501 TCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATA 7560
AGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTAT
7561 AGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGA 7620
TCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACT
7621 CCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCATAGACAGAAAGCGGT 7680
GGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCA
7681 ACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCT 7740
TGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGA
7741 ATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGA 7800
TACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCT
7801 AGAGGAAGGTGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCG 7860
TCTCCTTCCACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGC
attB1 other(7905,7929)>>>
|
NotI EcoRV
| | |
7861 CGGCCGCTTCGCCAGGGATCCGTCGACTTGACGCGTTGATATCAACAAGTTTGTACAAAA 7920
GCCGGCGAAGCGGTCCCTAGGCAGCTGAACTGCGCAACTATAGTTGTTCAAACATGTTTT
7921 AAGCAGGCTACAAAGAGGCCAGCGGTTCCGGACGGGCTGACGCATTGGACGATTTTGATC 7980
TTCGTCCGATGTTTCTCCGGTCGCCAAGGCCTGCCCGACTGCGTAACCTGCTAAAACTAG
7981 TGGATATGCTGGGAAGTGACGCCCTCGATGATTTTGACCTTGACATGCTTGGTTCGGATG 8040
ACCTATACGACCCTTCACTGCGGGAGCTACTAAAACTGGAACTGTACGAACCAAGCCTAC
8041 CCCTTGATGACTTTGACCTCGACATGCTCGGCAGTGACGCCCTTGATGATTTCGACCTGG 8100
GGGAACTACTGAAACTGGAGCTGTACGAGCCGTCACTGCGGGAACTACTAAAGCTGGACC
8101 ACATGCTGATTAACTCAAGAAGTTCCGGATCTCCGAAAAAGAAACGCAAAGTTGGTAGCC 8160
TGTACGACTAATTGAGTTCTTCAAGGCCTAGAGGCTTTTTCTTTGCGTTTCAACCATCGG
8161 AGTACCTGCCCGACACCGACGACCGGCACCGGATCGAGGAAAAGCGGAAGCGGACCTACG 8220
TCATGGACGGGCTGTGGCTGCTGGCCGTGGCCTAGCTCCTTTTCGCCTTCGCCTGGATGC
8221 AGACATTCAAGAGCATCATGAAGAAGTCCCCCTTCAGCGGCCCCACCGACCCTAGACCTC 8280
TCTGTAAGTTCTCGTAGTACTTCTTCAGGGGGAAGTCGCCGGGGTGGCTGGGATCTGGAG
8281 CACCTAGAAGAATCGCCGTGCCCAGCAGATCCAGCGCCAGCGTGCCAAAACCTGCCCCCC 8340
GTGGATCTTCTTAGCGGCACGGGTCGTCTAGGTCGCGGTCGCACGGTTTTGGACGGGGGG
8341 AGCCTTACCCCTTCACCAGCAGCCTGAGCACCATCAACTACGACGAGTTCCCTACCATGG 8400
TCGGAATGGGGAAGTGGTCGTCGGACTCGTGGTAGTTGATGCTGCTCAAGGGATGGTACC
8401 TGTTCCCCAGCGGCCAGATCTCTCAGGCCTCTGCTCTGGCTCCAGCCCCTCCTCAGGTGC 8460
ACAAGGGGTCGCCGGTCTAGAGAGTCCGGAGACGAGACCGAGGTCGGGGAGGAGTCCACG
8461 TGCCTCAGGCTCCTGCTCCTGCACCAGCTCCAGCCATGGTGTCTGCACTGGCTCAGGCAC 8520
ACGGAGTCCGAGGACGAGGACGTGGTCGAGGTCGGTACCACAGACGTGACCGAGTCCGTG
8521 CAGCACCCGTGCCTGTGCTGGCTCCTGGACCTCCACAGGCTGTGGCTCCACCAGCCCCTA 8580
GTCGTGGGCACGGACACGACCGAGGACCTGGAGGTGTCCGACACCGAGGTGGTCGGGGAT
8581 AACCTACACAGGCCGGCGAGGGCACACTGTCTGAAGCTCTGCTGCAGCTGCAGTTCGACG 8640
TTGGATGTGTCCGGCCGCTCCCGTGTGACAGACTTCGAGACGACGTCGACGTCAAGCTGC
8641 ACGAGGATCTGGGAGCCCTGCTGGGAAACAGCACCGATCCTGCCGTGTTCACCGACCTGG 8700
TGCTCCTAGACCCTCGGGACGACCCTTTGTCGTGGCTAGGACGGCACAAGTGGCTGGACC
8701 CCAGCGTGGACAACAGCGAGTTCCAACAGCTGCTGAACCAGGGCATCCCTGTGGCCCCTC 8760
GGTCGCACCTGTTGTCGCTCAAGGTTGTCGACGACTTGGTCCCGTAGGGACACCGGGGAG
8761 ACACCACCGAGCCCATGCTGATGGAATACCCCGAGGCCATCACCCGGCTCGTGACAGGCG 8820
TGTGGTGGCTCGGGTACGACTACCTTATGGGGCTCCGGTAGTGGGCCGAGCACTGTCCGC
8821 CTCAGAGGCCTCCTGATCCAGCTCCTGCCCCTCTGGGAGCACCAGGCCTGCCTAATGGAC 8880
GAGTCTCCGGAGGACTAGGTCGAGGACGGGGAGACCCTCGTGGTCCGGACGGATTACCTG
8881 TGCTGTCTGGCGACGAGGACTTCAGCTCTATCGCCGATATGGATTTCTCAGCCTTGCTGG 8940
ACGACAGACCGCTGCTCCTGAAGTCGAGATAGCGGCTATACCTAAAGAGTCGGAACGACC
8941 GCTCTGGCAGCGGCAGCCGGGATTCCAGGGAAGGGATGTTTTTGCCGAAGCCTGAGGCCG 9000
CGAGACCGTCGCCGTCGGCCCTAAGGTCCCTTCCCTACAAAAACGGCTTCGGACTCCGGC
9001 GCTCCGCTATTAGTGACGTGTTTGAGGGCCGCGAGGTGTGCCAGCCAAAACGAATCCGGC 9060
CGAGGCGATAATCACTGCACAAACTCCCGGCGCTCCACACGGTCGGTTTTGCTTAGGCCG
9061 CATTTCATCCTCCAGGAAGTCCATGGGCCAACCGCCCACTCCCCGCCAGCCTCGCACCAA 9120
GTAAAGTAGGAGGTCCTTCAGGTACCCGGTTGGCGGGTGAGGGGCGGTCGGAGCGTGGTT
9121 CACCAACCGGTCCAGTACATGAGCCAGTCGGGTCACTGACCCCGGCACCAGTCCCTCAGC 9180
GTGGTTGGCCAGGTCATGTACTCGGTCAGCCCAGTGACTGGGGCCGTGGTCAGGGAGTCG
9181 CACTGGATCCAGCGCCCGCAGTGACTCCCGAGGCCAGTCACCTGTTGGAGGATCCCGATG 9240
GTGACCTAGGTCGCGGGCGTCACTGAGGGCTCCGGTCAGTGGACAACCTCCTAGGGCTAC
9241 AAGAGACGAGCCAGGCTGTCAAAGCCCTTCGGGAGATGGCCGATACTGTGATTCCCCAGA 9300
TTCTCTGCTCGGTCCGACAGTTTCGGGAAGCCCTCTACCGGCTATGACACTAAGGGGTCT
9301 AGGAAGAGGCTGCAATCTGTGGCCAAATGGACCTTTCCCATCCGCCCCCAAGGGGCCATC 9360
TCCTTCTCCGACGTTAGACACCGGTTTACCTGGAAAGGGTAGGCGGGGGTTCCCCGGTAG
9361 TGGATGAGCTGACAACCACACTTGAGTCCATGACCGAGGATCTGAACCTGGACTCACCCC 9420
ACCTACTCGACTGTTGGTGTGAACTCAGGTACTGGCTCCTAGACTTGGACCTGAGTGGGG
9421 TGACCCCGGAATTGAACGAGATTCTGGATACCTTCCTGAACGACGAGTGCCTCTTGCATG 9480
ACTGGGGCCTTAACTTGCTCTAAGACCTATGGAAGGACTTGCTGCTCACGGAGAACGTAC
9481 CCATGCATATCAGCACAGGACTGTCCATCTTCGACACATCTCTGTTTACTAGTCCAAAAA 9540
GGTACGTATAGTCGTGTCCTGACAGGTAGAAGCTGTGTAGAGACAAATGATCAGGTTTTT
SV40 prom(9572,9840)>>>
|
9541 AGAAAAGAAAGGTGTGAGTTTAAACACGCGTGGTGTGGAAAGTCCCCAGGCTCCCCAGCA 9600
TCTTTTCTTTCCACACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGT
9601 GGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCA 9660
CCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGT
9661 GGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTC 9720
CCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAG
SV40 origin(9739,9816)>>>
|
9721 CCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCC 9780
GGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGG
SfiI
|
ORF_2 rf(2)(9806,10474)>>>
| |
9781 CATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTA 9840
GTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGAT
puro marker(9875,10474)>>>
|
9841 TTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCATGACCGAGTACAAGCCCACGGTGCG 9900
AAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGC
9901 CCTCGCCACCCGCGACGACGTCCCTCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGA 9960
GGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCT
9961 CTACCCCGCCACGCGCCACACCGTGGACCCGGACCGCCACATCGAGCGGGTCACCGAGCT 10020
GATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGA
10021 GCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGA 10080
CGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCT
10081 CGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGC 10140
GCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCG
10141 CGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGAT 10200
GCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTA
10201 GGAAGGGCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGG 10260
CCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCC
10261 CGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGA 10320
GCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCT
10321 GGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCC 10380
CCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGG
10381 CTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCG 10440
GAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGC
10441 CACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTCCGGAAATCAACCTCTGGA 10500
GTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCT
10501 TTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATG 10560
AATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATAC
10561 TGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTT 10620
ACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAA
10621 CTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAG 10680
GAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTC
10681 GCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGC 10740
CGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACG
10741 CACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGA 10800
GTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCT
10801 ACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAA 10860
TGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTT
10861 TTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCAC 10920
AAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTG
10921 CTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCT 10980
GACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGA
10981 TCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTCGCCTTCGCCCTCAG 11040
AGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTC
delta_U3 other(11084,11136)>>>
|
11041 ACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCCGGATGGAAGGGCTAATTCAC 11100
TGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTG
HIV-1_5_LTR other(11137,11317)>>>
|
11101 TCCCAACGAATACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATC 11160
AGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAG
11161 TGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTG 11220
ACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAAC
11221 CCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCC 11280
GGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGG
11281 CTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATT 11340
GAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAA
11341 ATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATT 11400
TAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAA
11401 GCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTT 11460
CGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAA
11461 TTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGG 11520
AAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACC
11521 CATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCATTATGGGTATTATGGGTCTG 11580
GTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGAC
11581 CATTAATGAATCGGCCAACGATCCCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA 11640
GTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTT
11641 TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG 11700
ATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCC
11701 CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG 11760
GACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCC
11761 GATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAG 11820
CTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTC
pBR322 origin(11823,12442)<<<
|
11821 GCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA 11880
CGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCT
11881 CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT 11940
GCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGA
11941 GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC 12000
CCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGG
12001 TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCG 12060
AAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGC
12061 GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC 12120
CACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCG
12121 TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA 12180
ACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGT
12181 CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG 12240
GACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTC
12241 TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCT 12300
AAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGA
12301 CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACC 12360
GACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGG
12361 ACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGA 12420
TGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCT
12421 TCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA 12480
AGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGT
12481 CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT 12540
GCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTA
12541 TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAC 12600
ATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATG
12601 CAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTT 12660
GTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAA
12661 GCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGT 12720
CGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCA
12721 GCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAG 12780
CGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTC
12781 CCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCT 12840
GGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGA
12841 ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT 12900
TAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAA
12901 GTTGAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGT 12960
CAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCA
12961 GCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAA 13020
CGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTT
13021 AGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTAT 13080
TCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATA
NEOKAN prom(13095,13144)>>>
|
13081 GGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCT 13140
CCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGA
13141 GCAAAGTAAACTGGATGGCTTTCTCGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCT 13200
CGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGA
NTP_II marker(13236,14024)>>>
|
ORF_4 rf(3)(13233,14027)>>>
| |
13201 CTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAG 13260
GACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTC
13261 GTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCG 13320
CAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGC
13321 GCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCA 13380
CGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGT
13381 AGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGC 13440
TCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCG
13441 TGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGG 13500
ACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCC
13501 ACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTG 13560
TGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGAC
13561 CCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTA 13620
GGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGAT
13621 CCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAG 13680
GGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTC
13681 CCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAAC 13740
GGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTG
13741 TGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCG 13800
ACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGC
13801 ATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTG 13860
TACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACAC
13861 GCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTG 13920
CGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGAC
13921 AAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCG 13980
TTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGC
13981 ATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTTTGTTAAAAT 14040
TAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTA
14041 TTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAACATCCCTTATAAA 14100
AAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTT
f1 origin(14132,14437)<<<
|
14101 TCAAAAGAATAGACCGCGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTA 14160
AGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGAT
14161 TTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCA 14220
AATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGT
14221 CTACGTGAACCATCACCCAAATCAAGTTTTTTGCGGTCGAGGTGCCGTAAAGCTCTAAAT 14280
GATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTA
14281 CGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCG 14340
GCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGC
14341 AGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC 14400
TCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAG
14401 ACGCTGCGCGTAACCACCACACCCGCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTC 14460
TGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAG
14461 GCCATTCAGGATCGAATTAATTCTTAATTAACATCATCAATAATATACCTT 14511
CGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA