pLenti-E1A-E1B
Vector Name | pLenti-E1A-E1B |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcagc caccatgaga catattatct gccacggagg tgttattacc gaagaaatgg 3121 ccgccagtct tttggaccag ctgatcgaag aggtactggc tgataatctt ccacctccta 3181 gccattttga accacctacc cttcacgaac tgtatgattt agacgtgacg gcccccgaag 3241 atcccaacga ggaggcggtt tcgcagattt ttcccgactc tgtaatgttg gcggtgcagg 3301 aagggattga cttactcact tttccgccgg cgcccggttc tccggagccg cctcaccttt 3361 cccggcagcc cgagcagccg gagcagagag ccttgggtcc ggtttctatg ccaaaccttg 3421 taccggaggt gatcgatctt acctgccacg aggctggctt tccacccagt gacgacgagg 3481 atgaagaggg tcctgtgtct gaacctgagc ctgagcccga gccagaaccg gagcctgcaa 3541 gacctacccg ccgtcctaaa atggcgcctg ctatcctgag acgcccgaca tcacctgtgt 3601 ctagagaatg caatagtagt acggatagct gtgactccgg tccttctaac acacctcctg 3661 agatacaccc ggtggtcccg ctgtgcccca ttaaaccagt tgccgtgaga gttggtgggc 3721 gtcgccaggc tgtggaatgt atcgaggact tgcttaacga gcctgggcaa cctttggact 3781 tgagctgtaa acgccccagg ccagagggca gaggaagtct tctaacatgc ggtgacgtgg 3841 aggagaatcc cggccctatg gaggcttggg agtgtttgga agatttttct gctgtgcgta 3901 acttgctgga acagagctct aacagtacct cttggttctg gaggtttctg tggggctcat 3961 cccaggcaaa gttagtctgc agaattaagg aggattacaa gtgggaattc gaagagcttt 4021 tgaagtcctg tggtgagctg tttgattctt tgaatctggg tcaccaggcg cttctccaag 4081 agaaggtcat caagactttg gatttttcca caccggggcg cgctgcggct gctgttgctt 4141 ttttgagttt tataaaggat aaatggagcg aagaaaccca tctgagcggg gggtacctgc 4201 tggattttct ggccatgcat ctgtggagag cggttgtgag acacaagaat cgcctgctac 4261 tgttgtcttc cgtccgcccg gcgataatac cgacggagga gcagcagcag cagcaggagg 4321 aagccaggcg gcggcggcag gagcagagcc catggaaccc gagagccggc ctggaccctc 4381 gggaatgact cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg 4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4741 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt 4801 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga 4861 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4921 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4981 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5041 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga 5101 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5161 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 5221 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 5281 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 5341 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5761 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5881 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 5941 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt 6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6361 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga 6421 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa 6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 7741 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca 7801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 7861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 7921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 7981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa 8041 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 8101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 8161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 8221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 8281 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 8341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 8401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 8461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 8521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 8581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 8641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 8701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 8761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 8821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 8881 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 8941 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt 9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg 9121 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct 9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta 9301 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca 9361 tcatcaataa tatacctt
LOCUS dna 9378 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4388 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3432..3437 /dbxref="REBASE:ClaI" misc_binding 3600..3605 /dbxref="REBASE:XbaI" misc_binding 3977..3982 /dbxref="REBASE:PstI" misc_binding 4005..4010 /dbxref="REBASE:EcoRI" misc_binding 4192..4197 /dbxref="REBASE:KpnI" misc_binding 4389..4394 /dbxref="REBASE:XhoI" Promoter 4439..4707 /gene="SV40 prom" Rep_Origin 4606..4683 /gene="SV40 origin" ORF 4673..5341 /sequence="ORF_2 rf(2)" misc_binding 4686..4698 /dbxref="REBASE:SfiI" Marker 4742..5341 /gene="puro marker" Other Gene 5951..6003 /gene="delta_U3 other" Other Gene 6004..6184 /gene="HIV-1_5_LTR other" Rep_Origin 6690..7309 /gene="pBR322 origin" Promoter 7962..8011 /gene="NEOKAN prom" misc_binding 8069..8074 /dbxref="REBASE:BclI" ORF 8100..8894 /sequence="ORF_4 rf(3)" Marker 8103..8891 /gene="NTP_II marker" Rep_Origin 8999..9304 /gene="f1 origin" BASE COUNT 2322 a 2322 c 2560 g 2174 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcagc caccatgaga catattatct gccacggagg tgttattacc gaagaaatgg 3121 ccgccagtct tttggaccag ctgatcgaag aggtactggc tgataatctt ccacctccta 3181 gccattttga accacctacc cttcacgaac tgtatgattt agacgtgacg gcccccgaag 3241 atcccaacga ggaggcggtt tcgcagattt ttcccgactc tgtaatgttg gcggtgcagg 3301 aagggattga cttactcact tttccgccgg cgcccggttc tccggagccg cctcaccttt 3361 cccggcagcc cgagcagccg gagcagagag ccttgggtcc ggtttctatg ccaaaccttg 3421 taccggaggt gatcgatctt acctgccacg aggctggctt tccacccagt gacgacgagg 3481 atgaagaggg tcctgtgtct gaacctgagc ctgagcccga gccagaaccg gagcctgcaa 3541 gacctacccg ccgtcctaaa atggcgcctg ctatcctgag acgcccgaca tcacctgtgt 3601 ctagagaatg caatagtagt acggatagct gtgactccgg tccttctaac acacctcctg 3661 agatacaccc ggtggtcccg ctgtgcccca ttaaaccagt tgccgtgaga gttggtgggc 3721 gtcgccaggc tgtggaatgt atcgaggact tgcttaacga gcctgggcaa cctttggact 3781 tgagctgtaa acgccccagg ccagagggca gaggaagtct tctaacatgc ggtgacgtgg 3841 aggagaatcc cggccctatg gaggcttggg agtgtttgga agatttttct gctgtgcgta 3901 acttgctgga acagagctct aacagtacct cttggttctg gaggtttctg tggggctcat 3961 cccaggcaaa gttagtctgc agaattaagg aggattacaa gtgggaattc gaagagcttt 4021 tgaagtcctg tggtgagctg tttgattctt tgaatctggg tcaccaggcg cttctccaag 4081 agaaggtcat caagactttg gatttttcca caccggggcg cgctgcggct gctgttgctt 4141 ttttgagttt tataaaggat aaatggagcg aagaaaccca tctgagcggg gggtacctgc 4201 tggattttct ggccatgcat ctgtggagag cggttgtgag acacaagaat cgcctgctac 4261 tgttgtcttc cgtccgcccg gcgataatac cgacggagga gcagcagcag cagcaggagg 4321 aagccaggcg gcggcggcag gagcagagcc catggaaccc gagagccggc ctggaccctc 4381 gggaatgact cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg 4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4741 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt 4801 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga 4861 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4921 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4981 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5041 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga 5101 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5161 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 5221 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 5281 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 5341 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5761 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5881 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 5941 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt 6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6361 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga 6421 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa 6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 7741 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca 7801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 7861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 7921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 7981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa 8041 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 8101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 8161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 8221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 8281 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 8341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 8401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 8461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 8521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 8581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 8641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 8701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 8761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 8821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 8881 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 8941 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt 9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg 9121 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct 9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta 9301 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca 9361 tcatcaataa tatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT ORF_3 rf(3)(2901,4388)>>> | CMV prom(2885,2966)>>> | | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC 3061 agctagcaGCCACCATGAGACATATTATCTGCCACGGAGGTGTTATTACCGAAGAAATGG 3120 TCGATCGTCGGTGGTACTCTGTATAATAGACGGTGCCTCCACAATAATGGCTTCTTTACC 3121 CCGCCAGTCTTTTGGACCAGCTGATCGAAGAGGTACTGGCTGATAATCTTCCACCTCCTA 3180 GGCGGTCAGAAAACCTGGTCGACTAGCTTCTCCATGACCGACTATTAGAAGGTGGAGGAT 3181 GCCATTTTGAACCACCTACCCTTCACGAACTGTATGATTTAGACGTGACGGCCCCCGAAG 3240 CGGTAAAACTTGGTGGATGGGAAGTGCTTGACATACTAAATCTGCACTGCCGGGGGCTTC 3241 ATCCCAACGAGGAGGCGGTTTCGCAGATTTTTCCCGACTCTGTAATGTTGGCGGTGCAGG 3300 TAGGGTTGCTCCTCCGCCAAAGCGTCTAAAAAGGGCTGAGACATTACAACCGCCACGTCC 3301 AAGGGATTGACTTACTCACTTTTCCGCCGGCGCCCGGTTCTCCGGAGCCGCCTCACCTTT 3360 TTCCCTAACTGAATGAGTGAAAAGGCGGCCGCGGGCCAAGAGGCCTCGGCGGAGTGGAAA 3361 CCCGGCAGCCCGAGCAGCCGGAGCAGAGAGCCTTGGGTCCGGTTTCTATGCCAAACCTTG 3420 GGGCCGTCGGGCTCGTCGGCCTCGTCTCTCGGAACCCAGGCCAAAGATACGGTTTGGAAC ClaI | 3421 TACCGGAGGTGATCGATCTTACCTGCCACGAGGCTGGCTTTCCACCCAGTGACGACGAGG 3480 ATGGCCTCCACTAGCTAGAATGGACGGTGCTCCGACCGAAAGGTGGGTCACTGCTGCTCC 3481 ATGAAGAGGGTCCTGTGTCTGAACCTGAGCCTGAGCCCGAGCCAGAACCGGAGCCTGCAA 3540 TACTTCTCCCAGGACACAGACTTGGACTCGGACTCGGGCTCGGTCTTGGCCTCGGACGTT 3541 GACCTACCCGCCGTCCTAAAATGGCGCCTGCTATCCTGAGACGCCCGACATCACCTGTGT 3600 CTGGATGGGCGGCAGGATTTTACCGCGGACGATAGGACTCTGCGGGCTGTAGTGGACACA XbaI | 3601 CTAGAGAATGCAATAGTAGTACGGATAGCTGTGACTCCGGTCCTTCTAACACACCTCCTG 3660 GATCTCTTACGTTATCATCATGCCTATCGACACTGAGGCCAGGAAGATTGTGTGGAGGAC 3661 AGATACACCCGGTGGTCCCGCTGTGCCCCATTAAACCAGTTGCCGTGAGAGTTGGTGGGC 3720 TCTATGTGGGCCACCAGGGCGACACGGGGTAATTTGGTCAACGGCACTCTCAACCACCCG 3721 GTCGCCAGGCTGTGGAATGTATCGAGGACTTGCTTAACGAGCCTGGGCAACCTTTGGACT 3780 CAGCGGTCCGACACCTTACATAGCTCCTGAACGAATTGCTCGGACCCGTTGGAAACCTGA 3781 TGAGCTGTAAACGCCCCAGGCCAGagggcagaggaagtcttctaacatgcggtgacgtgg 3840 ACTCGACATTTGCGGGGTCCGGTCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACC 3841 aggagaatcccggccctatggaggcttgggagtgtttggaagatttttctgctgtgcgta 3900 TCCTCTTAGGGCCGGGATACCTCCGAACCCTCACAAACCTTCTAAAAAGACGACACGCAT 3901 acttgctggaacagagctctaacagtacctcttggttctggaggtttctgtggggctcat 3960 TGAACGACCTTGTCTCGAGATTGTCATGGAGAACCAAGACCTCCAAAGACACCCCGAGTA PstI EcoRI | | 3961 cccaggcaaagttagtctgcagaattaaggaggattacaagtgggaattcgaagagcttt 4020 GGGTCCGTTTCAATCAGACGTCTTAATTCCTCCTAATGTTCACCCTTAAGCTTCTCGAAA 4021 tgaagtcctgtggtgagctgtttgattctttgaatctgggtcaccaggcgcttctccaag 4080 ACTTCAGGACACCACTCGACAAACTAAGAAACTTAGACCCAGTGGTCCGCGAAGAGGTTC 4081 agaaggtcatcaagactttggatttttccacaccggggcgcgctgcggctgctgttgctt 4140 TCTTCCAGTAGTTCTGAAACCTAAAAAGGTGTGGCCCCGCGCGACGCCGACGACAACGAA KpnI | 4141 ttttgagttttataaaggataaatggagcgaagaaacccatctgagcggggggtacctgc 4200 AAAACTCAAAATATTTCCTATTTACCTCGCTTCTTTGGGTAGACTCGCCCCCCATGGACG 4201 tggattttctggccatgcatctgtggagagcggttgtgagacacaagaatcgcctgctac 4260 ACCTAAAAGACCGGTACGTAGACACCTCTCGCCAACACTCTGTGTTCTTAGCGGACGATG 4261 tgttgtcttccgtccgcccggcgataataccgacggaggagcagcagcagcagcaggagg 4320 ACAACAGAAGGCAGGCGGGCCGCTATTATGGCTGCCTCCTCGTCGTCGTCGTCGTCCTCC 4321 aagccaggcggcggcggcaggagcagagcccatggaacccgagagccggcctggaccctc 4380 TTCGGTCCGCCGCCGCCGTCCTCGTCTCGGGTACCTTGGGCTCTCGGCCGGACCTGGGAG XhoI SV40 prom(4439,4707)>>> | | 4381 gggaatgactcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtgg 4440 CCCTTACTGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACC 4441 tgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattag 4500 ACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATC 4501 tcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatg 4560 AGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTAC SV40 origin(4606,4683)>>> | 4561 catctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaact 4620 GTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGA ORF_2 rf(2)(4673,5341)>>> | 4621 ccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagag 4680 GGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTC SfiI | 4681 gccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggc 4740 CGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCG puro marker(4742,5341)>>> | 4741 catgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgt 4800 GTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCA 4801 acgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccgga 4860 TGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCT 4861 ccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcga 4920 GGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCT 4921 catcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccgga 4980 GTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCT 4981 gagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcgg 5040 CTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCC 5041 ttcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaagga 5100 AAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCT 5101 gcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctggg 5160 CGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCC 5161 cagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcct 5220 GTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGA 5221 ggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgc 5280 CCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCG 5281 cgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctg 5340 GCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGAC 5341 aacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattct 5400 TTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGA 5401 taactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgc 5460 ATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACG 5461 tattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctct 5520 ATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGA 5521 ttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctga 5580 AATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACT 5581 cgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgc 5640 GCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCG 5641 tttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggac 5700 AAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTG 5701 aggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctt 5760 TCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAA 5761 tccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgt 5820 AGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCA 5821 cccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcc 5880 GGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGG 5881 tcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccg 5940 AGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGC delta_U3 other(5951,6003)>>> | 5941 cctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgt 6000 GGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACA HIV-1_5_LTR other(6004,6184)>>> | 6001 actgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaac 6060 TGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTG 6061 ccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctg 6120 GGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGAC 6121 ttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctct 6180 AACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGA 6181 agcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaata 6240 TCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTAT 6241 tcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagc 6300 AGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCG 6301 atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaa 6360 TAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTT 6361 ctcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatga 6420 GAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACT 6421 aatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaa 6480 TTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTT 6481 ataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc 6540 TATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAG 6541 actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg 6600 TGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGC 6601 gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc 6660 CATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCG pBR322 origin(6690,7309)<<< | 6661 cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc 6720 GTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCG 6721 ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga 6780 GGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCT 6781 ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc 6840 GATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGG 6841 ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat 6900 GACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTA 6901 agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg 6960 TCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACAC 6961 cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc 7020 GTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGG 7021 aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga 7080 TTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCT 7081 gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact 7140 CGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGA 7141 agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt 7200 TCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAA 7201 ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag 7260 CCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTC 7261 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg 7320 GTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCC 7321 tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa 7380 AGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTT 7381 aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata 7440 TCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATAT 7441 tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcg 7500 ATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGC 7501 atctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgata 7560 TAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTAT 7561 cgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccg 7620 GCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGC 7621 gctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct 7680 CGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGA 7681 gcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt 7740 CGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCA 7741 tcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttca 7800 AGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGT 7801 cgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggcta 7860 GCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGAT 7861 tctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacat 7920 AGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTA NEOKAN prom(7962,8011)>>> | 7921 ggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctgggg 7980 CCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCC 7981 cgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaa 8040 GCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTT BclI | 8041 ggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgca 8100 CCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGT NTP_II marker(8103,8891)>>> | ORF_4 rf(3)(8100,8894)>>> | | 8101 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcg 8160 ACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGC 8161 gctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcag 8220 CGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTC 8221 cgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgc 8280 GCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACG 8281 aagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgc 8340 TTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACG 8341 tcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcagg 8400 AGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCC 8401 atctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgc 8460 TAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACG 8461 ggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgca 8520 CCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGT 8521 tcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaag 8580 AGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTC 8581 agcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacg 8640 TCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGC 8641 gcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatg 8700 CGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTAC 8701 gccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggaca 8760 CGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGT 8761 tagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcc 8820 ATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGG 8821 tcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttg 8880 AGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAAC 8881 acgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaatag 8940 TGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATC f1 origin(8999,9304)<<< | 8941 gccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgtt 9000 CGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAA 9001 gttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcga 9060 CAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCT 9061 aaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttg 9120 TTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAAC 9121 cggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagct 9180 GCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGA 9181 tgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggc 9240 ACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCG 9241 gctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgctta 9300 CGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAAT 9301 atgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaaca 9360 TACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGT 9361 tcatcaataatatacctt 9378 AGTAGTTATTATATGGAA
LOCUS dna 9378 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4388 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3432..3437 /dbxref="REBASE:ClaI" misc_binding 3600..3605 /dbxref="REBASE:XbaI" misc_binding 3977..3982 /dbxref="REBASE:PstI" misc_binding 4005..4010 /dbxref="REBASE:EcoRI" misc_binding 4192..4197 /dbxref="REBASE:KpnI" misc_binding 4389..4394 /dbxref="REBASE:XhoI" Promoter 4439..4707 /gene="SV40 prom" Rep_Origin 4606..4683 /gene="SV40 origin" ORF 4673..5341 /sequence="ORF_2 rf(2)" misc_binding 4686..4698 /dbxref="REBASE:SfiI" Marker 4742..5341 /gene="puro marker" Other Gene 5951..6003 /gene="delta_U3 other" Other Gene 6004..6184 /gene="HIV-1_5_LTR other" Rep_Origin 6690..7309 /gene="pBR322 origin" Promoter 7962..8011 /gene="NEOKAN prom" misc_binding 8069..8074 /dbxref="REBASE:BclI" ORF 8100..8894 /sequence="ORF_4 rf(3)" Marker 8103..8891 /gene="NTP_II marker" Rep_Origin 8999..9304 /gene="f1 origin" BASE COUNT 2322 a 2322 c 2560 g 2174 t 0 others