pLenti-CMV-GFP-2A-Puro
| Vector Name | pLenti-CMV-GFP-2A-Puro |
| VectorType | Plasmid |
| Antibiotic Information | Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa
361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa
421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag
1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga
1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca
2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa
2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctagggttcc gcgttacata
2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
2641 atgggacttt cctacttggc agtacatcta cgtttagtca tcgctattac catggtgatg
2701 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
2761 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
2821 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
2881 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
2941 tgttttgacc tccatagaag aaccgagttt aaactcccta tcagtgatag agatctccct
3001 atcagtgata gagagctagc cccgggatcg atcaattgag tacttacgta ggtaccccag
3061 tgtggtggcc tgcaggtgaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg
3121 cggccgctgg atcctctaga ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
3361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
3421 gtgaggaggc ttttttggag gcctgtcgag gccaccatgg agagcgacga gagcggcctg
3481 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
3541 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
3601 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
3661 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
3721 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
3781 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
3841 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
3901 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
3961 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
4021 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
4081 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
4141 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
4201 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
4261 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
4321 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
4381 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
4441 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
4501 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
4561 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
4621 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
4681 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
4741 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
4801 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
4861 gcctgaacgc gttccggaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
4921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
4981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
5101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
5161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
5221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
5281 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
5341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
5401 cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
5461 ccccgcctgt ccggatggaa gggctaattc actcccaacg aatacaagat ctgctttttg
5521 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
5581 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
5641 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
5701 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
5761 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
5821 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
5881 ccaaactcat caatgtatct tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt
5941 aatgaaatgg cattatgggt attatgggtc tgcattaatg aatcggccaa cggatgtcgg
6001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
6061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
6121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
6181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
6241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
6301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
6361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
6421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
6481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
6541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
6601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
6661 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
6721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
6781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
6841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggc catgagatta
6901 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
6961 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
7021 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
7081 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
7141 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
7201 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
7261 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
7321 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
7381 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
7441 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
7501 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
7561 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
7621 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
7681 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
7741 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
7801 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
7861 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
7921 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
7981 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
8041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
8101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
8161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
8221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
8281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
8341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
8401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
8461 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt