pLenti-CMV-DIO-Blank-SV40-GFP
| Vector Name | pLenti-CMV-DIO-Blank-SV40-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt 3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta 3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg 3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt 3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3781 ggaggctttt ttggaggccg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 3841 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 3901 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 3961 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 4021 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 4081 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 4141 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 4201 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 4261 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 4321 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 4381 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 4441 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 4501 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4561 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 4981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5101 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 5161 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct 5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5401 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5461 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5581 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa 5641 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt 5701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6961 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt 7021 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg 7081 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta 7141 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg 7201 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc 7261 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc 7321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 7501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 8101 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa 8161 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt 8221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 8281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt 8341 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga 8401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 8461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc 8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta 8581 acatcatcaa taatatacct t
LOCUS dna 8601 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" misc_binding 3261..3266 /dbxref="REBASE:EcoRV" Other Gene 3383..3416 /gene="loxP other" misc_binding 3435..3440 /dbxref="REBASE:XbaI" Promoter 3497..3765 /gene="SV40 prom" Rep_Origin 3664..3741 /gene="SV40 origin" ORF 3731..4564 /sequence="ORF_2 rf(2)" misc_binding 3744..3756 /dbxref="REBASE:SfiI" misc_binding 4390..4395 /dbxref="REBASE:ApaI" Other Gene 5174..5226 /gene="delta_U3 other" Other Gene 5227..5407 /gene="HIV-1_5_LTR other" Rep_Origin 5913..6532 /gene="pBR322 origin" Promoter 7185..7234 /gene="NEOKAN prom" misc_binding 7292..7297 /dbxref="REBASE:BclI" ORF 7323..8117 /sequence="ORF_3 rf(3)" Marker 7326..8114 /gene="NTP_II marker" Rep_Origin 8222..8527 /gene="f1 origin" BASE COUNT 2218 a 2114 c 2256 g 2013 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt 3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta 3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg 3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt 3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3781 ggaggctttt ttggaggccg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 3841 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 3901 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 3961 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 4021 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 4081 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 4141 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 4201 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 4261 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 4321 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 4381 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 4441 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 4501 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4561 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 4981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5101 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 5161 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct 5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5401 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5461 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5581 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa 5641 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt 5701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6961 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt 7021 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg 7081 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta 7141 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg 7201 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc 7261 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc 7321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 7501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 8101 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa 8161 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt 8221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 8281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt 8341 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga 8401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 8461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc 8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta 8581 acatcatcaa taatatacct t //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC SmaI | XmaI ClaI KpnI | | | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT 3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180 ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG loxP other(3218,3251)<<< | 3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240 TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT EcoRV | 3241 tacgaagttatctcgagtcagatatcgctagccaattgataacttcgtataggatacttt 3300 ATGCTTCAATAGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAA 3301 atacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctcta 3360 TATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGAT loxP other(3383,3416)>>> | 3361 gtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatatgg 3420 CAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACC XbaI | 3421 tgagcaagggcgattctagactgcagctcgagtacccatacgacgtcccagactacgctt 3480 ACTCGTTCCCGCTAAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAA SV40 prom(3497,3765)>>> | 3481 gagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa 3540 CTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTT 3541 agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggc 3600 TCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCG 3601 agaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccg 3660 TCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGC SV40 origin(3664,3741)>>> | 3661 cccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatt 3720 GGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAA SfiI | ORF_2 rf(2)(3731,4564)>>> | | 3721 ttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtga 3780 AAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACT 3781 ggaggcttttttggaggccgccaccAtggagagcgacgagagcggcctgcccgccatgga 3840 CCTCCGAAAAAACCTCCGGCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCT 3841 gatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcgg 3900 CTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCGCC 3901 agagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccct 3960 TCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGA 3961 gaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcac 4020 CTGGAAGTCGGGGATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCGTG 4021 ctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaa 4080 GATGGGGTCGCCGATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTT 4081 cacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgcta 4140 GTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCGAT 4141 cgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacag 4200 GCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTC 4201 cgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccat 4260 GCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTA 4261 gggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggcta 4320 CCCGCTATTGCACGACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGAT 4321 ctacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcct 4380 GATGTCGAAGCACCACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGA ApaI | 4381 gcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccga 4440 CGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCT 4441 gctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccg 4500 CGACCCGTAGCACCTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGC 4501 cgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcg 4560 GCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGC 4561 ctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtat 4620 GACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATA 4621 tcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatca 4680 AGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGT 4681 tgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtc 4740 ACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAG 4741 tctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgc 4800 AGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACG 4801 tgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggacttt 4860 ACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAA 4861 cgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctg 4920 GCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGAC 4921 gacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtc 4980 CTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAG 4981 ctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgcta 5040 GAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGAT 5041 cgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcg 5100 GCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGC 5101 gcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctcc 5160 CGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGG delta_U3 other(5174,5226)>>> | 5161 ccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgct 5220 GGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGA HIV-1_5_LTR other(5227,5407)>>> | 5221 tgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactaggg 5280 ACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCC 5281 aacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgt 5340 TTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCA 5341 ctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatc 5400 GACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAG 5401 tctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatga 5460 AGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACT 5461 atatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaat 5520 TATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTA 5521 agcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtcc 5580 TCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGG 5581 aaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaa 5640 TTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATT 5641 tgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgt 5700 ACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACA 5701 gaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcg 5760 CTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGC 5761 ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaag 5820 GAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTC 5821 gcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaa 5880 CGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTT pBR322 origin(5913,6532)<<< | 5881 ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctc 5940 CCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAG 5941 cgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgaca 6000 GCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGT 6001 ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg 6060 CCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGC 6061 accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttct 6120 TGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGA 6121 catagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt 6180 GTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACA 6181 gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgag 6240 CACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTC 6241 tccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagc 6300 AGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCG 6301 agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctac 6360 TCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATG 6361 actagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaaga 6420 TGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCT 6421 gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc 6480 CAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACG 6481 aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacg 6540 TTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGC 6541 gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca 6600 CCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGT 6601 aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt 6660 TTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCA 6661 atatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctca 6720 TATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGT 6721 gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg 6780 CGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGC 6781 atacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctca 6840 TATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGT 6841 ccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt 6900 GGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCA 6901 cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagt 6960 GGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCA 6961 agttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttt 7020 TCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAA 7021 tcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactggg 7080 AGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCC 7081 ctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggctta 7140 GATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAAT NEOKAN prom(7185,7234)>>> | 7141 catggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctg 7200 GTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGAC 7201 gggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgc 7260 CCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCG BclI | 7261 caaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttc 7320 GTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAG NTP_II marker(7326,8114)>>> | ORF_3 rf(3)(7323,8117)>>> | | 7321 gcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctat 7380 CGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATA 7381 tcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgt 7440 AGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACA 7441 cagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaac 7500 GTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTG 7501 tgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctg 7560 ACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGAC 7561 tgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggc 7620 ACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCG 7621 aggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaa 7680 TCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTT 7681 tgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatc 7740 ACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAG 7741 gcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacg 7800 CGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGC 7801 aagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccg 7860 TTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGC 7861 acggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaa 7920 TGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTT 7921 atggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcagg 7980 TACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCC 7981 acatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgct 8040 TGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGA 8041 tcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttc 8100 AGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAG 8101 ttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaa 8160 AACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTT 8161 taggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagt 8220 ATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCA f1 origin(8222,8527)<<< | 8221 gttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaaggg 8280 CAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCC 8281 cgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtttt 8340 GCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAA 8341 ttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttaga 8400 AACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCT 8401 gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcg 8460 CGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGC 8461 ggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgc 8520 CCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCG 8521 ttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaatta 8580 AATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAAT 8581 acatcatcaataatatacctt 8601 TGTAGTAGTTATTATATGGAA
LOCUS dna 8601 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" misc_binding 3261..3266 /dbxref="REBASE:EcoRV" Other Gene 3383..3416 /gene="loxP other" misc_binding 3435..3440 /dbxref="REBASE:XbaI" Promoter 3497..3765 /gene="SV40 prom" Rep_Origin 3664..3741 /gene="SV40 origin" ORF 3731..4564 /sequence="ORF_2 rf(2)" misc_binding 3744..3756 /dbxref="REBASE:SfiI" misc_binding 4390..4395 /dbxref="REBASE:ApaI" Other Gene 5174..5226 /gene="delta_U3 other" Other Gene 5227..5407 /gene="HIV-1_5_LTR other" Rep_Origin 5913..6532 /gene="pBR322 origin" Promoter 7185..7234 /gene="NEOKAN prom" misc_binding 7292..7297 /dbxref="REBASE:BclI" ORF 7323..8117 /sequence="ORF_3 rf(3)" Marker 7326..8114 /gene="NTP_II marker" Rep_Origin 8222..8527 /gene="f1 origin" BASE COUNT 2218 a 2114 c 2256 g 2013 t 0 others