pLenti-CMV-DIO-GFP-SV40-Puro
| Vector Name | pLenti-CMV-DIO-GFP-SV40-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag
3301 ccgggtccgg cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag
3361 gcgatggggg tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc
3421 agctcctcca cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg
3481 atggcgctct tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc
3541 aggctgaagg tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc
3601 tccacggtgg cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag
3661 ccggtgccca ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag
3721 ctcacgtgca gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg
3781 ttgttgatgg cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag
3841 aagccgtagc ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg
3901 ctcttcatct tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc
3961 tcgaactcca cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg
4021 ccgctctcgt cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa
4081 cttcgtatag gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc
4141 atcaccatcg accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca
4201 ttatacgaag ttatatggtg agcaagggcg attctagact gcagctcgag tacccatacg
4261 acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg gaaagtcccc aggctcccca
4321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
4381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
4441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4561 ctattccaga agtagtgagg aggctttttt ggaggccatg accgagtaca agcccacggt
4621 gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc
4681 cgactacccc gccacgcgcc acaccgtgga cccggaccgc cacatcgagc gggtcaccga
4741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
4801 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
4861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
4921 gatggaaggg ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
4981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
5041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
5101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
5161 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttccggaa atcaacctct
5221 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
5281 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
5341 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
5401 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
5461 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
5521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
5581 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
5641 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
5701 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct
5761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt
5821 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
5881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
5941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
6001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
6061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
6241 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt
6301 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga
6361 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7621 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
7681 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
7741 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
7801 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
7861 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa
7921 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
7981 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
8041 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
8101 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
8161 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
8221 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
8281 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
8341 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
8401 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
8461 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
8521 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
8581 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
8641 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
8701 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
8761 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat
8821 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca
8881 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
8941 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta
9001 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
9061 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
9121 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca
9181 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
LOCUS dna 9234 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
ORF 3231..4133
/sequence="ORF_3 rf(3)"
ORF 3282..4040
/sequence="ORF_5 rf(5)"
misc_binding 3451..3456
/dbxref="REBASE:ApaI"
Other Gene 4181..4214
/gene="loxP other"
misc_binding 4233..4238
/dbxref="REBASE:XbaI"
Promoter 4295..4563
/gene="SV40 prom"
Rep_Origin 4462..4539
/gene="SV40 origin"
ORF 4529..5197
/sequence="ORF_2 rf(2)"
misc_binding 4542..4554
/dbxref="REBASE:SfiI"
Marker 4598..5197
/gene="puro marker"
Other Gene 5807..5859
/gene="delta_U3 other"
Other Gene 5860..6040
/gene="HIV-1_5_LTR other"
Rep_Origin 6546..7165
/gene="pBR322 origin"
Promoter 7818..7867
/gene="NEOKAN prom"
misc_binding 7925..7930
/dbxref="REBASE:BclI"
ORF 7956..8750
/sequence="ORF_4 rf(3)"
Marker 7959..8747
/gene="NTP_II marker"
Rep_Origin 8855..9160
/gene="f1 origin"
BASE COUNT 2272 a 2302 c 2519 g 2141 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag
3301 ccgggtccgg cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag
3361 gcgatggggg tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc
3421 agctcctcca cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg
3481 atggcgctct tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc
3541 aggctgaagg tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc
3601 tccacggtgg cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag
3661 ccggtgccca ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag
3721 ctcacgtgca gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg
3781 ttgttgatgg cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag
3841 aagccgtagc ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg
3901 ctcttcatct tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc
3961 tcgaactcca cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg
4021 ccgctctcgt cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa
4081 cttcgtatag gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc
4141 atcaccatcg accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca
4201 ttatacgaag ttatatggtg agcaagggcg attctagact gcagctcgag tacccatacg
4261 acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg gaaagtcccc aggctcccca
4321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
4381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
4441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4561 ctattccaga agtagtgagg aggctttttt ggaggccatg accgagtaca agcccacggt
4621 gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc
4681 cgactacccc gccacgcgcc acaccgtgga cccggaccgc cacatcgagc gggtcaccga
4741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
4801 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
4861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
4921 gatggaaggg ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
4981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
5041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
5101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
5161 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttccggaa atcaacctct
5221 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
5281 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
5341 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
5401 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
5461 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
5521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
5581 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
5641 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
5701 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct
5761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt
5821 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
5881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
5941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
6001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
6061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
6241 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt
6301 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga
6361 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7621 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
7681 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
7741 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
7801 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
7861 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa
7921 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
7981 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
8041 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
8101 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
8161 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
8221 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
8281 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
8341 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
8401 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
8461 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
8521 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
8581 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
8641 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
8701 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa
8761 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat
8821 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca
8881 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
8941 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta
9001 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
9061 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
9121 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca
9181 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI KpnI
| | | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT
3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180
ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG
ORF_3 rf(3)(3231,4133)>>>
|
loxP other(3218,3251)<<<
| |
3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240
TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT
ORF_5 rf(5)(3282,4040)<<<
|
3241 tacgaagttatctcgagCAAGTTTGTACAAAAAAGCAGGCTtcagcgagatccggtggag 3300
ATGCTTCAATAGAGCTCGTTCAAACATGTTTTTTCGTCCGAAGTCGCTCTAGGCCACCTC
3301 ccgggtccggcggtgccgtccacggcagaattggacgactgagcgcgggatctggcgaag 3360
GGCCCAGGCCGCCACGGCAGGTGCCGTCTTAACCTGCTGACTCGCGCCCTAGACCGCTTC
3361 gcgatgggggtcttgaaggcgtgctggtactccacgatgcccagctcggtgttgctgtgc 3420
CGCTACCCCCAGAACTTCCGCACGACCATGAGGTGCTACGGGTCGAGCCACAACGACACG
ApaI
|
3421 agctcctccacgcggcggaaggcgaacatggggcccccgttctgcaggatgctggggtgg 3480
TCGAGGAGGTGCGCCGCCTTCCGCTTGTACCCCGGGGGCAAGACGTCCTACGACCCCACC
3481 atggcgctcttgaagtgcatgtggctgtccaccacgaagctgtagtagccgccgtcgcgc 3540
TACCGCGAGAACTTCACGTACACCGACAGGTGGTGCTTCGACATCATCGGCGGCAGCGCG
3541 aggctgaaggtgcgggcgaagctgcccaccagcacgttatcgcccatggggtgcaggtgc 3600
TCCGACTTCCACGCCCGCTTCGACGGGTGGTCGTGCAATAGCGGGTACCCCACGTCCACG
3601 tccacggtggcgttgctgcggatgatcttgtcggtgaagatcacgctgtcctcggggaag 3660
AGGTGCCACCGCAACGACGCCTACTAGAACAGCCACTTCTAGTGCGACAGGAGCCCCTTC
3661 ccggtgcccaccaccttgaagtcgccgatcacgcggccggcctcgtagcggtagctgaag 3720
GGCCACGGGTGGTGGAACTTCAGCGGCTAGTGCGCCGGCCGGAGCATCGCCATCGACTTC
3721 ctcacgtgcagcacgccgccgtcctcgtacttctcgatgcgggtgttggtgtagccgccg 3780
GAGTGCACGTCGTGCGGCGGCAGGAGCATGAAGAGCTACGCCCACAACCACATCGGCGGC
3781 ttgttgatggcgtgcaggaaggggttctcgtagccgctggggtaggtgccgaagtggtag 3840
AACAACTACCGCACGTCCTTCCCCAAGAGCATCGGCGACCCCATCCACGGCTTCACCATC
3841 aagccgtagcccatcacgtggctcagcaggtaggggctgaaggtcagggcgcctttggtg 3900
TTCGGCATCGGGTAGTGCACCGAGTCGTCCATCCCCGACTTCCAGTCCCGCGGAAACCAC
3901 ctcttcatcttgttggtcatgcggccctgcttgggggtgccctctccgccgcccaccagc 3960
GAGAAGTAGAACAACCAGTACGCCGGGACGAACCCCCACGGGAGAGGCGGCGGGTGGTCG
3961 tcgaactccacgccgttcagggtgccggtgatgcggcactcgatctccatggcgggcagg 4020
AGCTTGAGGTGCGGCAAGTCCCACGGCCACTACGCCGTGAGCTAGAGGTACCGCCCGTCC
4021 ccgctctcgtcgctctccaTACCCAGCTTTCTTGTACAAAGTGGgctagccaattgataa 4080
GGCGAGAGCAGCGAGAGGTATGGGTCGAAAGAACATGTTTCACCCGATCGGTTAACTATT
4081 cttcgtataggatactttatacgaagttatcattgggattcttcctattttgatccaagc 4140
GAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCG
loxP other(4181,4214)>>>
|
4141 atcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagcataca 4200
TAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGT
XbaI
|
4201 ttatacgaagttatatggtgagcaagggcgattctagactgcagctcgagtacccatacg 4260
AATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTGACGTCGAGCTCATGGGTATGC
SV40 prom(4295,4563)>>>
|
4261 acgtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctcccca 4320
TGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGT
4321 gcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtcc 4380
CGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGG
4381 ccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccata 4440
GGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTAT
SV40 origin(4462,4539)>>>
|
4441 gtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccg 4500
CAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGC
SfiI
|
ORF_2 rf(2)(4529,5197)>>>
| |
4501 ccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgag 4560
GGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTC
puro marker(4598,5197)>>>
|
4561 ctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggt 4620
GATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCA
4621 gcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgc 4680
CGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCG
4681 cgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccga 4740
GCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCT
4741 gctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcgga 4800
CGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCT
4801 cgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgtt 4860
GCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAA
4861 cgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaaca 4920
GCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGT
4921 gatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgt 4980
CTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCA
4981 cggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagt 5040
GCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCA
5041 ggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacct 5100
CCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGA
5101 ccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggacc 5160
GGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGG
5161 gcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctct 5220
CGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGA
5221 ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgct 5280
CCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGA
5281 atgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcat 5340
TACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTA
5341 tttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgt 5400
AAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACA
5401 caggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcat 5460
GTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTA
5461 tgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggc 5520
ACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCG
5521 ggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactga 5580
CCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACT
5581 caattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgc 5640
GTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACG
5641 cacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcgga 5700
GTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCT
5701 ccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccct 5760
GGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGA
delta_U3 other(5807,5859)>>>
|
5761 cagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaatt 5820
GTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAA
HIV-1_5_LTR other(5860,6040)>>>
|
5821 cactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccag 5880
GTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTC
5881 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 5940
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG
5941 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 6000
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT
6001 tccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatctt 6060
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAA
6061 attattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgttt 6120
TAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAA
6121 attgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagca 6180
TAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGT
6181 tttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtc 6240
AAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAG
6241 tggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggt 6300
ACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCA
6301 ctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggaga 6360
GACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCT
6361 aaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt 6420
TTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAA
6421 cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatca 6480
GCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGT
6481 ggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaa 6540
CCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTT
pBR322 origin(6546,7165)<<<
|
6541 aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat 6600
TTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTA
6601 cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc 6660
GCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGG
6661 cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc 6720
GGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGG
6721 gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagt 6780
CGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCA
6781 tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac 6840
AGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTG
6841 cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg 6900
GCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGC
6901 ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca 6960
GGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGT
6961 gagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgc 7020
CTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACG
7021 gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa 7080
CGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTT
7081 accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaa 7140
TGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTT
7141 ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac 7200
CCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTG
7201 tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta 7260
AGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAT
7261 aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt 7320
TTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCA
7321 taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccata 7380
ATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTAT
7381 gttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc 7440
CAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGG
7441 agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaac 7500
TCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTG
7501 cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag 7560
GTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTC
7561 tctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac 7620
AGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTG
7621 gttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaac 7680
CAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTG
7681 ggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcg 7740
CCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGC
7741 caaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttt 7800
GTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAA
NEOKAN prom(7818,7867)>>>
|
7801 tatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc 7860
ATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCG
7861 cctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaa 7920
GGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTT
NTP_II marker(7959,8747)>>>
|
BclI ORF_4 rf(3)(7956,8750)>>>
| | |
7921 gctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacg 7980
CGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGC
7981 caggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaa 8040
GTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTT
8041 tcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttg 8100
AGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAAC
8101 tcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgt 8160
AGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCA
8161 ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaa 8220
CCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTT
8221 gggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctc 8280
CCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAG
8281 ctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccgg 8340
GACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCC
8341 ctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgg 8400
GATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACC
8401 aagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg 8460
TTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGC
8461 aactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatg 8520
TTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTAC
8521 gcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgact 8580
CGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGA
8581 gtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattg 8640
CACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAAC
8641 ctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctc 8700
GACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAG
8701 ccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaa 8760
GGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATT
8761 aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttat 8820
TTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATA
f1 origin(8855,9160)<<<
|
8821 aaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcca 8880
TTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGT
8881 ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc 8940
GATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCG
8941 ccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctcta 9000
GGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGAT
9001 aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg 9060
TTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCAC
9061 gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg 9120
CGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGC
9121 gtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcca 9180
CAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGT
9181 ttcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 9234
AAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9234 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
ORF 3231..4133
/sequence="ORF_3 rf(3)"
ORF 3282..4040
/sequence="ORF_5 rf(5)"
misc_binding 3451..3456
/dbxref="REBASE:ApaI"
Other Gene 4181..4214
/gene="loxP other"
misc_binding 4233..4238
/dbxref="REBASE:XbaI"
Promoter 4295..4563
/gene="SV40 prom"
Rep_Origin 4462..4539
/gene="SV40 origin"
ORF 4529..5197
/sequence="ORF_2 rf(2)"
misc_binding 4542..4554
/dbxref="REBASE:SfiI"
Marker 4598..5197
/gene="puro marker"
Other Gene 5807..5859
/gene="delta_U3 other"
Other Gene 5860..6040
/gene="HIV-1_5_LTR other"
Rep_Origin 6546..7165
/gene="pBR322 origin"
Promoter 7818..7867
/gene="NEOKAN prom"
misc_binding 7925..7930
/dbxref="REBASE:BclI"
ORF 7956..8750
/sequence="ORF_4 rf(3)"
Marker 7959..8747
/gene="NTP_II marker"
Rep_Origin 8855..9160
/gene="f1 origin"
BASE COUNT 2272 a 2302 c 2519 g 2141 t 0 others