pLenti-CMV-DIO-Blank-SV40-Puro
| Vector Name | pLenti-CMV-DIO-Blank-SV40-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: KanamycinMammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt 3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta 3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg 3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt 3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3781 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 3841 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 3901 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc 4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 4381 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga 4441 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4501 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4561 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4621 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 4681 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 4741 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 4801 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 4861 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 4921 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc 4981 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa 5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 5281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 5341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg 5461 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc 5521 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 5581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6181 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct 6841 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga 6901 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag 6961 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac 7021 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga 7081 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag 7141 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 7321 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 7681 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 7921 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc 7981 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc 8041 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 8161 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg 8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 8341 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga 8401 attaattctt aattaacatc atcaataata tacctt
LOCUS dna 8436 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" misc_binding 3261..3266 /dbxref="REBASE:EcoRV" Other Gene 3383..3416 /gene="loxP other" misc_binding 3435..3440 /dbxref="REBASE:XbaI" misc_binding 3441..3446 /dbxref="REBASE:PstI" Promoter 3497..3765 /gene="SV40 prom" Rep_Origin 3664..3741 /gene="SV40 origin" ORF 3731..4399 /sequence="ORF_2 rf(2)" misc_binding 3744..3756 /dbxref="REBASE:SfiI" Marker 3800..4399 /gene="puro marker" Other Gene 5009..5061 /gene="delta_U3 other" Other Gene 5062..5242 /gene="HIV-1_5_LTR other" Rep_Origin 5748..6367 /gene="pBR322 origin" Promoter 7020..7069 /gene="NEOKAN prom" misc_binding 7127..7132 /dbxref="REBASE:BclI" ORF 7158..7952 /sequence="ORF_3 rf(3)" Marker 7161..7949 /gene="NTP_II marker" Rep_Origin 8057..8362 /gene="f1 origin" BASE COUNT 2149 a 2071 c 2238 g 1978 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca 3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc 3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta 3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt 3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta 3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg 3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt 3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3781 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 3841 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 3901 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc 4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 4381 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga 4441 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4501 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4561 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4621 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 4681 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 4741 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 4801 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 4861 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 4921 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc 4981 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa 5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 5281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 5341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg 5461 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc 5521 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 5581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6181 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct 6841 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga 6901 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag 6961 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac 7021 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga 7081 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag 7141 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 7321 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 7681 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 7921 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc 7981 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc 8041 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 8161 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg 8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 8341 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga 8401 attaattctt aattaacatc atcaataata tacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC SmaI | XmaI ClaI KpnI | | | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT 3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180 ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG loxP other(3218,3251)<<< | 3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240 TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT EcoRV | 3241 tacgaagttatctcgagtcagatatcgctagccaattgataacttcgtataggatacttt 3300 ATGCTTCAATAGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAA 3301 atacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctcta 3360 TATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGAT loxP other(3383,3416)>>> | 3361 gtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatatgg 3420 CAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACC XbaI PstI | | 3421 tgagcaagggcgattctagactgcagctcgagtacccatacgacgtcccagactacgctt 3480 ACTCGTTCCCGCTAAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAA SV40 prom(3497,3765)>>> | 3481 gagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa 3540 CTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTT 3541 agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggc 3600 TCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCG 3601 agaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccg 3660 TCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGC SV40 origin(3664,3741)>>> | 3661 cccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatt 3720 GGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAA SfiI | ORF_2 rf(2)(3731,4399)>>> | | 3721 ttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtga 3780 AAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACT puro marker(3800,4399)>>> | 3781 ggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcga 3840 CCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCT 3841 cgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcg 3900 GCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGC 3901 ccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcct 3960 GGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGA 3961 cacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggc 4020 GTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCG 4021 ggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcg 4080 CCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGC 4081 catggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggc 4140 GTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCG 4141 gccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgacca 4200 CGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGT 4201 ccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgc 4260 GGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCG 4261 cggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggct 4320 GCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGA 4321 cggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgac 4380 GCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTG 4381 ccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtga 4440 GGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACT 4441 aagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgcttt 4500 TTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAA 4501 aatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataa 4560 TTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATT 4561 atcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggt 4620 TAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCA 4621 gtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagct 4680 CACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGA 4681 cctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctg 4740 GGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGAC 4741 ccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtc 4800 GGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAG 4801 ggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgg 4860 CCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCC 4861 gacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcct 4920 CTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGA 4921 gctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcc 4980 CGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGG delta_U3 other(5009,5061)>>> | 4981 ctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaa 5040 GAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTT HIV-1_5_LTR other(5062,5242)>>> | 5041 gatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctc 5100 CTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAG 5101 tctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaa 5160 AGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTT 5161 gtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttag 5220 CATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATC 5221 tcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataa 5280 AGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATT 5281 cttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggt 5340 GAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCA 5341 tacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattct 5400 ATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGA 5401 agttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtg 5460 TCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCAC 5461 cggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggc 5520 GCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCG 5521 caacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgc 5580 GTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCG 5581 tcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta 5640 AGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCAT 5641 tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaag 5700 AGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTC pBR322 origin(5748,6367)<<< | 5701 aacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcg 5760 TTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGC 5761 tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg 5820 AAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCC 5821 tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtg 5880 ACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCAC 5881 cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga 5940 GCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCT 5941 agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgc 6000 TCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCG 6001 tccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggt 6060 AGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCA 6061 aactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact 6120 TTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGA 6121 ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgg 6180 CCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACC 6181 cctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagtt 6240 GGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAA 6241 accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggt 6300 TGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCA 6301 ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct 6360 CCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGA 6361 ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg 6420 AACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAAC 6421 gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt 6480 CAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAA 6481 aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt 6540 TTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCA 6541 gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc 6600 CTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAG 6601 gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccg 6660 CACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGC 6661 cgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggcc 6720 GCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGG 6721 gagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg 6780 CTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCC 6781 gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatct 6840 CTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGA 6841 tcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatga 6900 AGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACT 6901 atgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtag 6960 TACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATC 6961 cttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaac 7020 GAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTG NEOKAN prom(7020,7069)>>> | 7021 cggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactgga 7080 GCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCT BclI | 7081 tggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacag 7140 ACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTC NTP_II marker(7161,7949)>>> | ORF_3 rf(3)(7158,7952)>>> | | 7141 gatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgctt 7200 CTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAA 7201 gggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccg 7260 CCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGC 7261 ccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccg 7320 GGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGC 7321 gtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcg 7380 CACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGC 7381 ttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgg 7440 AAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACC 7441 gcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcca 7500 CGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGT 7501 tcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgacc 7560 AGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGG 7561 accaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 7620 TGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAG 7621 aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctca 7680 TCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGT 7681 aggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccga 7740 TCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCT 7741 atatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtgg 7800 TATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACC 7801 cggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcg 7860 GCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGC 7861 aatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcg 7920 TTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGC 7921 ccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagc 7980 GGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCG 7981 tcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagacc 8040 AGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGG f1 origin(8057,8362)<<< | 8041 gcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggac 8100 CGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTG 8101 tccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatca 8160 AGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGT 8161 cccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaaggg 8220 GGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCC 8221 agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaag 8280 TCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTC 8281 aaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacc 8340 TTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGG 8341 accacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcga 8400 TGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCT 8401 attaattcttaattaacatcatcaataatatacctt 8436 TAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 8436 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3068..3073 /dbxref="REBASE:SmaI" misc_binding 3068..3073 /dbxref="REBASE:XmaI" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" Other Gene 3218..3251 /gene="loxP other" misc_binding 3261..3266 /dbxref="REBASE:EcoRV" Other Gene 3383..3416 /gene="loxP other" misc_binding 3435..3440 /dbxref="REBASE:XbaI" misc_binding 3441..3446 /dbxref="REBASE:PstI" Promoter 3497..3765 /gene="SV40 prom" Rep_Origin 3664..3741 /gene="SV40 origin" ORF 3731..4399 /sequence="ORF_2 rf(2)" misc_binding 3744..3756 /dbxref="REBASE:SfiI" Marker 3800..4399 /gene="puro marker" Other Gene 5009..5061 /gene="delta_U3 other" Other Gene 5062..5242 /gene="HIV-1_5_LTR other" Rep_Origin 5748..6367 /gene="pBR322 origin" Promoter 7020..7069 /gene="NEOKAN prom" misc_binding 7127..7132 /dbxref="REBASE:BclI" ORF 7158..7952 /sequence="ORF_3 rf(3)" Marker 7161..7949 /gene="NTP_II marker" Rep_Origin 8057..8362 /gene="f1 origin" BASE COUNT 2149 a 2071 c 2238 g 1978 t 0 others