pLenti-Bicistronic-Luc
| Vector Name | pLenti-Bicistronic-Luc |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 61 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 121 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 181 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 241 ctttcgacct atcgatcaat tgagtactta cgtaggtacc gaattcgata tcgggcccgc 301 ggccgcgtct agatgagtcg agtacccata cgacgtccca gactacgctt gagtttaaac 361 acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 421 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 481 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 661 ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtccct 721 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtg 781 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 841 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 901 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 961 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc gccgcaccgg 1021 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 1081 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 1141 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 1201 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 1261 ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga aagattgact 1321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 1381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 1441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 1501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 1561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 1621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 1681 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 1741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 1801 ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 1861 cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa gatctgcttt 1921 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 1981 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 2041 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 2101 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 2161 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 2221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 2281 tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg cggagaaaga 2341 ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc caacggatgt 2401 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggccatgaga 3301 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg 3361 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca 3421 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta 3481 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc 3541 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc 3601 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 3661 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 3721 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 3781 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 3841 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 3901 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 3961 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4021 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4081 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4141 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4201 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4261 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4321 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4381 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa 4441 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 4501 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 4561 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 4621 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 4681 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 4741 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 4801 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat 4861 tcgccattca ggatcgaatt aattcttaat taacatcatc aataatatac cttttttgga 4921 ttgaagccaa tatgataatg agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg 4981 gggcgggtga cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt 5041 aagcgacgga tgtggcaaaa gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac 5101 aattttcgcg cggttttagg cggatgttgt agtaaatttg ggcgtaaccg agtaagattt 5161 ggccattttc gcgggaaaac tgaataagag gaagtgaaat ctgaataatt ttgtgttact 5221 catagcgcgt aatactctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5281 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtaacta taacggtcct 5341 aaggtagcga aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg 5401 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta 5461 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga 5521 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac 5581 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5641 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5701 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5761 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 5821 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 5881 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 5941 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 6001 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 6061 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 6121 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 6181 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 6241 aaaacaaaag taagaccacc gcacagcaag cccgctgatc ttcagacctg gaggaggaga 6301 tatgagggac attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 6361 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 6421 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 6481 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 6541 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 6601 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 6661 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 6721 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 6781 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 6841 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 6901 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 6961 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 7021 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 7081 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg 7141 tatcgaaagc ttgggattcg aatttaaaag aaaagggggg attggggggt acagtgcagg 7201 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaactac aaaaacaaat 7261 tacaaaaatt caaaattttc gggtttttcg aacctagcag acgtcggcag tgaaaaaaat 7321 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 7381 aacaagttaa caacaagaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 7441 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctcacct 7501 aggctcgagt tacacggcga tcttgccgcc cttcttggcc ttaatgagaa tctcgcggat 7561 cttgcgggcg tccaacttgc cggtcagtcc tttaggcacc tcgtccacga acacaacacc 7621 accgcgcagc ttcttggcgg ttgtaacctg gctggccaca tagtccacga tctccttctc 7681 ggtcatggtt ttaccgtgtt ccagcacgac gactgcggcg ggcagctcgc cggcatcgtc 7741 gtcgggcagg ccggcgaccc cggcgtcgaa gatgttgggg tgttgcagca ggatgctctc 7801 cagttcggct ggggctacct ggtagccctt gtatttgatc aggctcttca gccggtccac 7861 gatgaagaag tgctcgtcct cgtcccagta ggcgatgtcg ccgctgtgca gccagccgtc 7921 cttgtcgatg agagcgtttg tagcctcggg gttgttaacg tagccgctca tgatcatggg 7981 gccacggacg cacagctcgc cgcgctggtt cacacccagt gtcttaccgg tgtccaagtc 8041 caccacctta gcctcgaaga agggcaccac cttgcctact gcgccaggct tgtcgtcccc 8101 ttcgggggtg atcagaatgg cgctggttgt ttctgtcagg ccgtagccct ggcggatgcc 8161 tggtaggtgg aagcgtttgg ccacggcctc acctacctcc ttgctgagcg gcgccccgcc 8221 gctggcgatc tcgtgcaagt tgcttaggtc gtacttgtcg atgagagtgc tcttagcgaa 8281 gaagctaaat agtgtgggca ccagcagggc agattgaatc ttatagtctt gcaagctgcg 8341 caagaatagc tcctcctcga agcggtacat gagcacgacc cgaaagccgc agatcaagta 8401 gcccagcgtg gtgaacatgc cgaagccgtg gtgaaatggc accacgctga ggatagcggt 8461 gtcggggatg atctggttgc cgaagatggg gtcgcgggca tgactgaatc ggacacaagc 8521 ggtgcggtgc ggtagggcta cgcccttggg caatccggta ctgccactac tgttcatgat 8581 cagggcgatg gttttgtccc ggtcgaagct ctcgggcacg aagtcgtact cgttgaagcc 8641 gggtggcaaa tgggaagtca cgaaggtgta catgctttgg aagccctggt agtcggtctt 8701 gctatccatg atgatgatct tttgtatgat cggtagcttc ttttgcacgt tgaggatctt 8761 ttgcagccct ttcttgctca cgaatacgac ggtgggctgg ctgatgccca tgctgttcag 8821 cagctcgcgc tcgttgtaga tgtcgttagc tggggccaca gccacaccga tgaacagggc 8881 acccaacacg ggcatgaaga actgcaagct attctcgctg cacaccacga tccgatggtt 8941 tgtattcagc ccatagcgct tcatagcttc tgccagccga acgctcatct cgaagtactc 9001 ggcgtaggta atgtccacct cgatatgtgc gtcggtaaag gcgatggtgc cgggcaccag 9061 ggcgtagcgc ttcatggctt tgtgcagctg ctcgccggcg gtcccgtctt cgagtgggta 9121 gaatggcgct gggcccttct taatgttttt ggcatcttcc atggtggcgg atcccccggg 9181 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgccacc 9241 tcgacataaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc 9301 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc 9361 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 9421 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 9481 acaaatggaa gtagcacgtc tcactagtct c
FEATURES Location/Qualifiers source 1..9511 /organism="recombinant plasmid" /mol_type="other DNA" CDS 323..349 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 534..669 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 670..1269 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 1282..1869 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(1753..1764) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 1752" /translation="IEGR" misc_feature 1879..2112 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 1932..2112 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 2184..2305 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(2634..3222) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" misc_feature 3566 /note="should be t" /note="color: #ff0000" CDS 3634..4428 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" LTR 5581..5761 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 5808..5933 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 6423..6656 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature complement(7510..9162) /note="Luciferase" /note="color: #ff0000; direction: LEFT" promoter complement(9181..9248) /note="miniCMV" /note="color: #ffffff; direction: LEFT" promoter 9258..246 /note="PGK promoter" /note="phosphoglycerate kinase promoter" /note="color: #ffffff; direction: RIGHT" ORIGIN 1 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 61 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 121 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 181 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 241 ctttcgacct atcgatcaat tgagtactta cgtaggtacc gaattcgata tcgggcccgc 301 ggccgcgtct agatgagtcg agtacccata cgacgtccca gactacgctt gagtttaaac 361 acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 421 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 481 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 661 ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtccct 721 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtg 781 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 841 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 901 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 961 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc gccgcaccgg 1021 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 1081 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 1141 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 1201 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 1261 ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga aagattgact 1321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 1381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 1441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 1501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 1561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 1621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 1681 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 1741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 1801 ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 1861 cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa gatctgcttt 1921 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 1981 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 2041 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 2101 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 2161 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 2221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 2281 tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg cggagaaaga 2341 ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc caacggatgt 2401 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggccatgaga 3301 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg 3361 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca 3421 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta 3481 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc 3541 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc 3601 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 3661 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 3721 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 3781 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 3841 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 3901 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 3961 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4021 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4081 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4141 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4201 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4261 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4321 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4381 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa 4441 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 4501 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 4561 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 4621 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 4681 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 4741 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 4801 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat 4861 tcgccattca ggatcgaatt aattcttaat taacatcatc aataatatac cttttttgga 4921 ttgaagccaa tatgataatg agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg 4981 gggcgggtga cgtagtagtg tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt 5041 aagcgacgga tgtggcaaaa gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac 5101 aattttcgcg cggttttagg cggatgttgt agtaaatttg ggcgtaaccg agtaagattt 5161 ggccattttc gcgggaaaac tgaataagag gaagtgaaat ctgaataatt ttgtgttact 5221 catagcgcgt aatactctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5281 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtaacta taacggtcct 5341 aaggtagcga aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg 5401 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta 5461 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga 5521 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac 5581 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5641 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5701 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5761 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 5821 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 5881 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 5941 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 6001 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 6061 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 6121 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 6181 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 6241 aaaacaaaag taagaccacc gcacagcaag cccgctgatc ttcagacctg gaggaggaga 6301 tatgagggac attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 6361 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 6421 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 6481 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 6541 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 6601 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 6661 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 6721 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 6781 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 6841 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 6901 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 6961 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 7021 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 7081 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg 7141 tatcgaaagc ttgggattcg aatttaaaag aaaagggggg attggggggt acagtgcagg 7201 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaactac aaaaacaaat 7261 tacaaaaatt caaaattttc gggtttttcg aacctagcag acgtcggcag tgaaaaaaat 7321 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 7381 aacaagttaa caacaagaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 7441 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctcacct 7501 aggctcgagt tacacggcga tcttgccgcc cttcttggcc ttaatgagaa tctcgcggat 7561 cttgcgggcg tccaacttgc cggtcagtcc tttaggcacc tcgtccacga acacaacacc 7621 accgcgcagc ttcttggcgg ttgtaacctg gctggccaca tagtccacga tctccttctc 7681 ggtcatggtt ttaccgtgtt ccagcacgac gactgcggcg ggcagctcgc cggcatcgtc 7741 gtcgggcagg ccggcgaccc cggcgtcgaa gatgttgggg tgttgcagca ggatgctctc 7801 cagttcggct ggggctacct ggtagccctt gtatttgatc aggctcttca gccggtccac 7861 gatgaagaag tgctcgtcct cgtcccagta ggcgatgtcg ccgctgtgca gccagccgtc 7921 cttgtcgatg agagcgtttg tagcctcggg gttgttaacg tagccgctca tgatcatggg 7981 gccacggacg cacagctcgc cgcgctggtt cacacccagt gtcttaccgg tgtccaagtc 8041 caccacctta gcctcgaaga agggcaccac cttgcctact gcgccaggct tgtcgtcccc 8101 ttcgggggtg atcagaatgg cgctggttgt ttctgtcagg ccgtagccct ggcggatgcc 8161 tggtaggtgg aagcgtttgg ccacggcctc acctacctcc ttgctgagcg gcgccccgcc 8221 gctggcgatc tcgtgcaagt tgcttaggtc gtacttgtcg atgagagtgc tcttagcgaa 8281 gaagctaaat agtgtgggca ccagcagggc agattgaatc ttatagtctt gcaagctgcg 8341 caagaatagc tcctcctcga agcggtacat gagcacgacc cgaaagccgc agatcaagta 8401 gcccagcgtg gtgaacatgc cgaagccgtg gtgaaatggc accacgctga ggatagcggt 8461 gtcggggatg atctggttgc cgaagatggg gtcgcgggca tgactgaatc ggacacaagc 8521 ggtgcggtgc ggtagggcta cgcccttggg caatccggta ctgccactac tgttcatgat 8581 cagggcgatg gttttgtccc ggtcgaagct ctcgggcacg aagtcgtact cgttgaagcc 8641 gggtggcaaa tgggaagtca cgaaggtgta catgctttgg aagccctggt agtcggtctt 8701 gctatccatg atgatgatct tttgtatgat cggtagcttc ttttgcacgt tgaggatctt 8761 ttgcagccct ttcttgctca cgaatacgac ggtgggctgg ctgatgccca tgctgttcag 8821 cagctcgcgc tcgttgtaga tgtcgttagc tggggccaca gccacaccga tgaacagggc 8881 acccaacacg ggcatgaaga actgcaagct attctcgctg cacaccacga tccgatggtt 8941 tgtattcagc ccatagcgct tcatagcttc tgccagccga acgctcatct cgaagtactc 9001 ggcgtaggta atgtccacct cgatatgtgc gtcggtaaag gcgatggtgc cgggcaccag 9061 ggcgtagcgc ttcatggctt tgtgcagctg ctcgccggcg gtcccgtctt cgagtgggta 9121 gaatggcgct gggcccttct taatgttttt ggcatcttcc atggtggcgg atcccccggg 9181 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgccacc 9241 tcgacataaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc 9301 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc 9361 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 9421 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 9481 acaaatggaa gtagcacgtc tcactagtct c
FEATURES Location/Qualifiers source 1..9511 /organism="recombinant plasmid" /mol_type="other DNA" CDS 323..349 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 534..669 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 670..1269 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 1282..1869 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(1753..1764) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 1752" /translation="IEGR" misc_feature 1879..2112 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 1932..2112 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 2184..2305 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(2634..3222) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" misc_feature 3566 /note="should be t" /note="color: #ff0000" CDS 3634..4428 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" LTR 5581..5761 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 5808..5933 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 6423..6656 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature complement(7510..9162) /note="Luciferase" /note="color: #ff0000; direction: LEFT" promoter complement(9181..9248) /note="miniCMV" /note="color: #ffffff; direction: LEFT" promoter 9258..246 /note="PGK promoter" /note="phosphoglycerate kinase promoter" /note="color: #ffffff; direction: RIGHT"