pLenti-Bi-cistronic
| Vector Name | pLenti-Bi-cistronic |
| VectorType | Lentivirus |
| Antibiotic Information | Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca1801 aaattttcgg gtttttcgaa cctagcagac gtcggcagtg aaaaaaatgc tttatttgtg1861 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca1921 acaagaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa1981 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctcacctag gctcgagcat2041 atggctagcg gatcccccgg gcgatctgac ggttcactaa acgagctctg cttatatagg2101 cctcccaccg tacacgccac ctcgacataa attctaccgg gtaggggagg cgcttttccc2161 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg2221 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg2281 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca2341 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat2401 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct2461 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag2521 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac2581 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac2641 ctatcgatca attgagtact tacgtaggta ccgaattcga tatcgggccc gcggccgcgt2701 ctagatgagt cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg2761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag2821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg2881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact2941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag3001 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc3061 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt3121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga3181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga3241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga3301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg3361 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga3421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg3481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct3541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc3601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg3661 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct3721 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc3781 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct3841 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga3901 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc3961 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac4021 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt4081 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt4141 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc4201 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg4261 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct4501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata4561 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc4621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa4681 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga4741 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacggat gtcggtgtga4801 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct4861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc4921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg4981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg5041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg5101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac5161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca5221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt5281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc5341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag5401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac5461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt5521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa5581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg5641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggccatga gattatcaaa5701 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc5761 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa5821 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc5881 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt5941 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca6001 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc6061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc6121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga6181 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac6241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct6301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa6361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc6421 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct6481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc6541 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg6601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct6661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct6721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca6781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt6841 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag6901 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga6961 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg7021 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc7081 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg7141 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc7201 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt7261 caggatcgaa ttaattctta attaacatca tcaataatat accttatttt ggattgaagc7321 caatatgata atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg7381 tgacgtagta gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac7441 ggatgtggca aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc7501 gcgcggtttt aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt7561 ttcgcgggaa aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg7621 cgtaatactc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg7681 gttccgcgca catttccccg aaaagtgcca cctgacgtaa ctataacggt cctaaggtag7741 cgaaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc7801 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt7861 acgat
FEATURES Location/Qualifiers source 1..7864 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature 1691..1807 /note="cPPT/CTS" /note="color: #ffe4c4" polyA_signal 1835..1916 /note="SV40 poly(A) signal" /note="color: #a6acb3" promoter complement(2062..2129) /note="miniCMV" /note="color: #ffffff; direction: LEFT" promoter 2139..2638 /note="PGK promoter" /note="color: #ffffff; direction: RIGHT" CDS 2715..2741 /codon_start=1 /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" promoter 2759..3056 /note="SV40 promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 2926..3061 /note="SV40 ori" /note="color: #ffff00" CDS 3062..3661 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="This feature has 2 segments: 1: 3062 .. 3196 / #ccffcc 2: 3197 .. 3661 / #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 3674..4261 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4145..4156) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4271..4504 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4324..4504 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 4576..4697 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5026..5614) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6026..6820 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagcagac gtcggcagtg aaaaaaatgc tttatttgtg 1861 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 1921 acaagaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 1981 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctcacctag gctcgagcat 2041 atggctagcg gatcccccgg gcgatctgac ggttcactaa acgagctctg cttatatagg 2101 cctcccaccg tacacgccac ctcgacataa attctaccgg gtaggggagg cgcttttccc 2161 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 2221 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 2281 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 2341 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 2401 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 2461 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 2521 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 2581 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 2641 ctatcgatca attgagtact tacgtaggta ccgaattcga tatcgggccc gcggccgcgt 2701 ctagatgagt cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg 2761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3001 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3061 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt 3121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga 3181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 3241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 3301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 3361 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga 3421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 3481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 3541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 3601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 3661 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 3721 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 3781 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 3841 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 3901 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 3961 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 4021 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 4081 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 4141 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 4201 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 4261 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt 4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 4501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 4561 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4681 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga 4741 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacggat gtcggtgtga 4801 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 4861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 4921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 4981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 5041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 5101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 5161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 5221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 5281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 5341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 5401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 5461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 5521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 5581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 5641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggccatga gattatcaaa 5701 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc 5761 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa 5821 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc 5881 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt 5941 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca 6001 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 6061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 6121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 6181 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 6241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 6301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 6361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 6421 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 6481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 6541 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 6601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 6661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 6721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 6781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt 6841 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 6901 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 6961 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 7021 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 7081 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 7141 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 7201 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt 7261 caggatcgaa ttaattctta attaacatca tcaataatat accttttttg gattgaagcc 7321 aatatgataa tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt 7381 gacgtagtag tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg 7441 gatgtggcaa aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg 7501 cgcggtttta ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt 7561 tcgcgggaaa actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc 7621 gtaatactct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 7681 ttccgcgcac atttccccga aaagtgccac ctgacgtaac tataacggtc ctaaggtagc 7741 gaaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 7801 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 7861 cgat
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca1801 aaattttcgg gtttttcgaa cctagcagac gtcggcagtg aaaaaaatgc tttatttgtg1861 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca1921 acaagaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa1981 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctcacctag gctcgagcat2041 atggctagcg gatcccccgg gcgatctgac ggttcactaa acgagctctg cttatatagg2101 cctcccaccg tacacgccac ctcgacataa attctaccgg gtaggggagg cgcttttccc2161 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg2221 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg2281 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca2341 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat2401 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct2461 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag2521 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac2581 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac2641 ctatcgatca attgagtact tacgtaggta ccgaattcga tatcgggccc gcggccgcgt2701 ctagatgagt cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg2761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag2821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg2881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact2941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag3001 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc3061 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt3121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga3181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga3241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga3301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg3361 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga3421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg3481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct3541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc3601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg3661 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct3721 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc3781 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct3841 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga3901 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc3961 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac4021 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt4081 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt4141 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc4201 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg4261 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct4501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata4561 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc4621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa4681 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga4741 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacggat gtcggtgtga4801 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct4861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc4921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg4981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg5041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg5101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac5161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca5221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt5281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc5341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag5401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac5461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt5521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa5581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg5641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggccatga gattatcaaa5701 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc5761 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa5821 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc5881 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt5941 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca6001 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc6061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc6121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga6181 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac6241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct6301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa6361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc6421 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct6481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc6541 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg6601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct6661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct6721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca6781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt6841 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag6901 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga6961 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg7021 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc7081 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg7141 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc7201 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt7261 caggatcgaa ttaattctta attaacatca tcaataatat accttatttt ggattgaagc7321 caatatgata atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg7381 tgacgtagta gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac7441 ggatgtggca aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc7501 gcgcggtttt aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt7561 ttcgcgggaa aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg7621 cgtaatactc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg7681 gttccgcgca catttccccg aaaagtgcca cctgacgtaa ctataacggt cctaaggtag7741 cgaaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc7801 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt7861 acgat
FEATURES Location/Qualifiers source 1..7864 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature 1691..1807 /note="cPPT/CTS" /note="color: #ffe4c4" polyA_signal 1835..1916 /note="SV40 poly(A) signal" /note="color: #a6acb3" promoter complement(2062..2129) /note="miniCMV" /note="color: #ffffff; direction: LEFT" promoter 2139..2638 /note="PGK promoter" /note="color: #ffffff; direction: RIGHT" CDS 2715..2741 /codon_start=1 /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" promoter 2759..3056 /note="SV40 promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 2926..3061 /note="SV40 ori" /note="color: #ffff00" CDS 3062..3661 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="This feature has 2 segments: 1: 3062 .. 3196 / #ccffcc 2: 3197 .. 3661 / #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 3674..4261 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4145..4156) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4271..4504 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4324..4504 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 4576..4697 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5026..5614) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6026..6820 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN