pLenti-3UTR-Dual-Luc

Vector Name	pLenti-3UTR-Dual-Luc
VectorType	Lentiviral Plasmid
Antibiotic Information	Bacterial: Kanamycin Mammalian: Puromycin
Sequencing Primers	Luciferase forward sequencing primer: 5'-GCAAGTTGGACGCCCGCAAGATC-3' SV40 reverse sequencing primer: 5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information	CMV driving expression of firefly luciferase (P. pyralis), followed by a MCS for cloning in a 3'UTR sequence. A second Renilla luciferase (R. reniformis) is driven by the SV40 promoter to allow for normalization.

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 
3061 agctagagct agcagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat 
3121 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg 
3181 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg 
3241 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata 
3301 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg 
3361 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc 
3421 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc 
3481 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata 
3541 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac 
3601 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc 
3661 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca 
3721 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg 
3781 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg 
3841 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct 
3901 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct 
3961 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca 
4021 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac 
4081 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg 
4141 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg 
4201 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg 
4261 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca 
4321 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca 
4381 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac 
4441 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg 
4501 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga 
4561 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg 
4621 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca 
4681 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg caattgtaag 
4741 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata 
4801 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag 
4861 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga 
4921 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
4981 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
5041 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
5101 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
5161 aagtagtgag gaggcttttt tggaggccga tatcgccacc atgttgttga aagttgtgtt 
5221 tgctattgga tgtatcgtag tgcaggctat ggcctcaaaa gtgtacgatc cggagcagcg 
5281 gaagaggatg atcacggggc cccaatggtg ggcacgatgc aagcagatga atgtgttgga 
5341 cagtttcatt aactactacg acagcgagaa acacgcggag aacgcagtga tattcctgca 
5401 cggcaatgca accagtagct atctgtggag acacgtggtg cctcatattg agccggtcgc 
5461 tagatgcatt attcccgatc ttattggaat ggggaaatcc ggaaagagtg gaaatggatc 
5521 atataggctc ctcgatcatt ataaatatct gactgcttgg tttgaattgc tcaatctgcc 
5581 caagaaaatc atctttgtag gacatgattg gggctccgcc cttgcttttc attatgccta 
5641 tgaacaccag gatcggatca aggctattgt tcacatggag agcgtggtgg atgtgattga 
5701 atcatggatg ggttggccgg atatagaaga agagctggcg ctgattaaat ctgaggaggg 
5761 cgagaagatg gtactcgaaa ataacttctt tgtcgagacg gtactgccca gtaagatcat 
5821 gcgcaaactg gagcctgaag agtttgcggc ttacctggaa cccttcaagg agaagggaga 
5881 ggtgaggaga ccgaccctgt catggcctcg ggaaattccg ctggtcaaag gagggaagcc 
5941 agacgtcgtc gccattgtcc ggaattacaa cgcttacctc cgcgctagtg acgacctgcc 
6001 taaactcttc atcgaatcag atcctggttt ctttagtaac gccatcgtcg agggcgccaa 
6061 gaagtttcca aacaccgaat ttgttaaagt caaaggactt cacttcctcc aggaggatgc 
6121 gcccgatgaa atgggaaagt atatcaaatc cttcgtggag agggtcttga agaatgagca 
6181 gaggtccatc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg 
6241 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc 
6301 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc 
6361 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 
6421 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 
6481 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 
6541 cggttcccgg ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa 
6601 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 
6661 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 
6721 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 
6781 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 
6841 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 
6901 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 
6961 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 
7021 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 
7081 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 
7141 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 
7201 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 
7261 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 
7321 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 
7381 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
7441 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct 
7501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
7561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
7621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
7681 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
7741 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
7801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
7861 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa 
7921 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt 
7981 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 
8041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
8101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
8161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
8221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
8281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
8341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
8401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
8461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
8521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
8581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
8641 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
8701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
8761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
8821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
8881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
8941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
9001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
9061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
9121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
9181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
9241 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt 
9301 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg 
9361 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta 
9421 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg 
9481 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc 
9541 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc 
9601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 
9661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 
9721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 
9781 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 
9841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 
9901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 
9961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 
10021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 
10081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 
10141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 
10201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 
10261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 
10321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 
10381 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa 
10441 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt 
10501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 
10561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt 
10621 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga 
10681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 
10741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc 
10801 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta 
10861 acatcatcaa taatatacct t

                            LOCUS dna 10881 bp 
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4739
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 4077..4934
/sequence="ORF_5 rf(5)"
misc_binding 4740..4745
/dbxref="REBASE:KpnI"
misc_binding 4767..4772
/dbxref="REBASE:EcoRI"
misc_binding 4785..4790
/dbxref="REBASE:StuI"
misc_binding 4809..4816
/dbxref="REBASE:NotI"
misc_binding 4818..4823
/dbxref="REBASE:BamHI"
misc_binding 4824..4829
/dbxref="REBASE:XbaI"
misc_binding 4836..4841
/dbxref="REBASE:XhoI"
Promoter 4886..5154
/gene="SV40 prom"
Rep_Origin 5053..5130
/gene="SV40 origin"
ORF 5120..6844
/sequence="ORF_2 rf(2)"
misc_binding 5133..5145
/dbxref="REBASE:SfiI"
Marker 6245..6844
/gene="puro marker"
Other Gene 7454..7506
/gene="delta_U3 other"
Other Gene 7507..7687
/gene="HIV-1_5_LTR other"
Rep_Origin 8193..8812
/gene="pBR322 origin"
Promoter 9465..9514
/gene="NEOKAN prom"
ORF 9603..10397
/sequence="ORF_4 rf(3)"
Marker 9606..10394
/gene="NTP_II marker"
Rep_Origin 10502..10807
/gene="f1 origin"
BASE COUNT 2729 a 2735 c 2970 g 2447 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 
3061 agctagagct agcagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat 
3121 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg 
3181 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg 
3241 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata 
3301 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg 
3361 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc 
3421 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc 
3481 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata 
3541 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac 
3601 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc 
3661 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca 
3721 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg 
3781 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg 
3841 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct 
3901 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct 
3961 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca 
4021 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac 
4081 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg 
4141 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg 
4201 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg 
4261 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca 
4321 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca 
4381 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac 
4441 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg 
4501 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga 
4561 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg 
4621 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca 
4681 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg caattgtaag 
4741 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata 
4801 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag 
4861 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga 
4921 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
4981 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
5041 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
5101 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
5161 aagtagtgag gaggcttttt tggaggccga tatcgccacc atgttgttga aagttgtgtt 
5221 tgctattgga tgtatcgtag tgcaggctat ggcctcaaaa gtgtacgatc cggagcagcg 
5281 gaagaggatg atcacggggc cccaatggtg ggcacgatgc aagcagatga atgtgttgga 
5341 cagtttcatt aactactacg acagcgagaa acacgcggag aacgcagtga tattcctgca 
5401 cggcaatgca accagtagct atctgtggag acacgtggtg cctcatattg agccggtcgc 
5461 tagatgcatt attcccgatc ttattggaat ggggaaatcc ggaaagagtg gaaatggatc 
5521 atataggctc ctcgatcatt ataaatatct gactgcttgg tttgaattgc tcaatctgcc 
5581 caagaaaatc atctttgtag gacatgattg gggctccgcc cttgcttttc attatgccta 
5641 tgaacaccag gatcggatca aggctattgt tcacatggag agcgtggtgg atgtgattga 
5701 atcatggatg ggttggccgg atatagaaga agagctggcg ctgattaaat ctgaggaggg 
5761 cgagaagatg gtactcgaaa ataacttctt tgtcgagacg gtactgccca gtaagatcat 
5821 gcgcaaactg gagcctgaag agtttgcggc ttacctggaa cccttcaagg agaagggaga 
5881 ggtgaggaga ccgaccctgt catggcctcg ggaaattccg ctggtcaaag gagggaagcc 
5941 agacgtcgtc gccattgtcc ggaattacaa cgcttacctc cgcgctagtg acgacctgcc 
6001 taaactcttc atcgaatcag atcctggttt ctttagtaac gccatcgtcg agggcgccaa 
6061 gaagtttcca aacaccgaat ttgttaaagt caaaggactt cacttcctcc aggaggatgc 
6121 gcccgatgaa atgggaaagt atatcaaatc cttcgtggag agggtcttga agaatgagca 
6181 gaggtccatc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg 
6241 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc 
6301 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc 
6361 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 
6421 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 
6481 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 
6541 cggttcccgg ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa 
6601 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 
6661 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 
6721 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 
6781 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 
6841 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 
6901 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 
6961 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 
7021 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 
7081 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 
7141 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 
7201 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 
7261 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 
7321 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 
7381 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
7441 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct 
7501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
7561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
7621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
7681 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
7741 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
7801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
7861 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa 
7921 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt 
7981 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 
8041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
8101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
8161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
8221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
8281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
8341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
8401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
8461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
8521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
8581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
8641 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
8701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
8761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
8821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
8881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
8941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
9001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
9061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
9121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
9181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
9241 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt 
9301 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg 
9361 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta 
9421 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg 
9481 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc 
9541 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc 
9601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 
9661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 
9721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 
9781 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 
9841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 
9901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 
9961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 
10021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 
10081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 
10141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 
10201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 
10261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 
10321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 
10381 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa 
10441 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt 
10501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 
10561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt 
10621 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga 
10681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 
10741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc 
10801 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta 
10861 acatcatcaa taatatacct t 
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

Encap other(162,310)>>> 
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

amp prom(365,393)<<< 
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC

541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC

601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA

HIV-1_5_LTR other(715,895)>>> 
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA

721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA

781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG

841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG

901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG

HIV-1_psi_pack other(1006,1050)>>> 
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT

1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC

NruI 
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT

1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG

1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT

1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG

1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT

1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC

1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT

RRE reg(1557,1790)>>> 
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT

ORF_1 rf(1)(1600,2301)>>> 
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC

1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC

1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG

1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC

1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA

1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG

1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA

1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT

2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT

2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC

2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT

2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT

2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC

2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT

2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA

2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA

2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT

NdeI 
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG

2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA

2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA

2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC

2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT

ORF_3 rf(3)(2901,4739)>>> 
|
CMV prom(2885,2966)>>> 
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT

2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG

tetO reg(3022,3061)>>> 
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC

3061 agctagagctagcagccaccatggaagatgccaaaaacattaagaagggcccagcgccat 3120
TCGATCTCGATCGTCGGTGGTACCTTCTACGGTTTTTGTAATTCTTCCCGGGTCGCGGTA

3121 tctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacg 3180
AGATGGGTGAGCTTCTGCCCTGGCGGCCGCTCGTCGACGTGTTTCGGTACTTCGCGATGC

3181 ccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacg 3240
GGGACCACGGGCCGTGGTAGCGGAAATGGCTGCGTGTATAGCTCCACCTGTAATGGATGC

3241 ccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaata 3300
GGCTCATGAAGCTCTACTCGCAAGCCGACCGTCTTCGATACTTCGCGATACCCGACTTAT

3301 caaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgg 3360
GTTTGGTAGCCTAGCACCACACGTCGCTCTTATCGAACGTCAAGAAGTACGGGCACAACC

3361 gtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagc 3420
CACGGGACAAGTAGCCACACCGACACCGGGGTCGATTGCTGTAGATGTTGCTCGCGCTCG

3421 tgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgc 3480
ACGACTTGTCGTACCCGTAGTCGGTCGGGTGGCAGCATAAGCACTCGTTCTTTCCCGACG

3481 aaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggata 3540
TTTTCTAGGAGTTGCACGTTTTCTTCGATGGCTAGTATGTTTTCTAGTAGTAGTACCTAT

3541 gcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccac 3600
CGTTCTGGCTGATGGTCCCGAAGGTTTCGTACATGTGGAAGCACTGAAGGGTAAACGGTG

3601 ccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccc 3660
GGCCGAAGTTGCTCATGCTGAAGCACGGGCTCTCGAAGCTGGCCCTGTTTTGGTAGCGGG

3661 tgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgca 3720
ACTAGTACTTGTCATCACCGTCATGGCCTAACGGGTTCCCGCATCGGGATGGCGTGGCGT

3721 ccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccg 3780
GGCGAACACAGGCTAAGTCAGTACGGGCGCTGGGGTAGAAGCCGTTGGTCTAGTAGGGGC

3781 acaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgg 3840
TGTGGCGATAGGAGTCGCACCACGGTAAAGTGGTGCCGAAGCCGTACAAGTGGTGCGACC

3841 gctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattct 3900
CGATGAACTAGACGCCGAAAGCCCAGCACGAGTACATGGCGAAGCTCCTCCTCGATAAGA

3901 tgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagct 3960
ACGCGTCGAACGTTCTGATATTCTAAGTTAGACGGGACGACCACGGGTGTGATAAATCGA

3961 tcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgcca 4020
AGAAGCGATTCTCGTGAGAGTAGCTGTTCATGCTGGATTCGTTGAACGTGCTCTAGCGGT

ORF_5 rf(5)(4077,4934)<<< 
|
4021 gcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctac 4080
CGCCGCCCCGCGGCGAGTCGTTCCTCCATCCACTCCGGCACCGGTTTGCGAAGGTGGATG

4081 caggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccg 4140
GTCCGTAGGCGGTCCCGATGCCGGACTGTCTTTGTTGGTCGCGGTAAGACTAGTGGGGGC

4141 aaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtgg 4200
TTCCCCTGCTGTTCGGACCGCGTCATCCGTTCCACCACGGGAAGAAGCTCCGATTCCACC

4201 tggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtg 4260
ACCTGAACCTGTGGCCATTCTGTGACCCACACTTGGTCGCGCCGCTCGACACGCAGGCAC

4261 gccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgaca 4320
CGGGGTACTAGTACTCGCCGATGCAATTGTTGGGGCTCCGATGTTTGCGAGAGTAGCTGT

4321 aggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttca 4380
TCCTGCCGACCGACGTGTCGCCGCTGTAGCGGATGACCCTGCTCCTGCTCGTGAAGAAGT

4381 tcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaac 4440
AGCACCTGGCCGACTTCTCGGACTAGTTTATGTTCCCGATGGTCCATCGGGGTCGGCTTG

4441 tggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccg 4500
ACCTCTCGTAGGACGACGTTGTGGGGTTGTAGAAGCTGCGGCCCCAGCGGCCGGACGGGC

4501 acgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatga 4560
TGCTGCTACGGCCGCTCGACGGGCGGCGTCAGCAGCACGACCTTGTGCCATTTTGGTACT

4561 ccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcg 4620
GGCTCTTCCTCTAGCACCTGATACACCGGTCGGTCCAATGTTGGCGGTTCTTCGACGCGC

4621 gtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgca 4680
CACCACAACACAAGCACCTGCTCCACGGATTTCCTGACTGGCCGTTCAACCTGCGGGCGT

4681 agatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgcaattgtaag 4740
TCTAGGCGCTCTAAGAGTAATTCCGGTTCTTCCCGCCGTTCTAGCGGCACGTTAACATTC

KpnI EcoRI StuI 
| | |
4741 gtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctgtcgacgata 4800
CATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTAT

NotI BamHI XbaI XhoI 
| | | |
4801 tcgggcccgcggccgctggatcctctagactgcagctcgagtacccatacgacgtcccag 4860
AGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTC

SV40 prom(4886,5154)>>> 
|
4861 actacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcaga 4920
TGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCT

4921 agtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcc 4980
TCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGG

4981 ccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgccc 5040
GGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGG

SV40 origin(5053,5130)>>> 
|
5041 ctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc 5100
GATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCG

SfiI 
|
ORF_2 rf(2)(5120,6844)>>> 
| |
5101 tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccag 5160
ACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTC

5161 aagtagtgaggaggcttttttggaggccgatatcgccaccATGTTGTTGAAAGTTGTGTT 5220
TTCATCACTCCTCCGAAAAAACCTCCGGCTATAGCGGTGGTACAACAACTTTCAACACAA

5221 TGCTATTGGATGTATCGTAGTGCAGGCTATGGCCTCAAAAGTGTACGATCCGGAGCAGCG 5280
ACGATAACCTACATAGCATCACGTCCGATACCGGAGTTTTCACATGCTAGGCCTCGTCGC

5281 GAAGAGGATGATCACGGGGCCCCAATGGTGGGCACGATGCAAGCAGATGAATGTGTTGGA 5340
CTTCTCCTACTAGTGCCCCGGGGTTACCACCCGTGCTACGTTCGTCTACTTACACAACCT

5341 CAGTTTCATTAACTACTACGACAGCGAGAAACACGCGGAGAACGCAGTGATATTCCTGCA 5400
GTCAAAGTAATTGATGATGCTGTCGCTCTTTGTGCGCCTCTTGCGTCACTATAAGGACGT

5401 CGGCAATGCAACCAGTAGCTATCTGTGGAGACACGTGGTGCCTCATATTGAGCCGGTCGC 5460
GCCGTTACGTTGGTCATCGATAGACACCTCTGTGCACCACGGAGTATAACTCGGCCAGCG

5461 TAGATGCATTATTCCCGATCTTATTGGAATGGGGAAATCCGGAAAGAGTGGAAATGGATC 5520
ATCTACGTAATAAGGGCTAGAATAACCTTACCCCTTTAGGCCTTTCTCACCTTTACCTAG

5521 ATATAGGCTCCTCGATCATTATAAATATCTGACTGCTTGGTTTGAATTGCTCAATCTGCC 5580
TATATCCGAGGAGCTAGTAATATTTATAGACTGACGAACCAAACTTAACGAGTTAGACGG

5581 CAAGAAAATCATCTTTGTAGGACATGATTGGGGCTCCGCCCTTGCTTTTCATTATGCCTA 5640
GTTCTTTTAGTAGAAACATCCTGTACTAACCCCGAGGCGGGAACGAAAAGTAATACGGAT

5641 TGAACACCAGGATCGGATCAAGGCTATTGTTCACATGGAGAGCGTGGTGGATGTGATTGA 5700
ACTTGTGGTCCTAGCCTAGTTCCGATAACAAGTGTACCTCTCGCACCACCTACACTAACT

5701 ATCATGGATGGGTTGGCCGGATATAGAAGAAGAGCTGGCGCTGATTAAATCTGAGGAGGG 5760
TAGTACCTACCCAACCGGCCTATATCTTCTTCTCGACCGCGACTAATTTAGACTCCTCCC

5761 CGAGAAGATGGTACTCGAAAATAACTTCTTTGTCGAGACGGTACTGCCCAGTAAGATCAT 5820
GCTCTTCTACCATGAGCTTTTATTGAAGAAACAGCTCTGCCATGACGGGTCATTCTAGTA

5821 GCGCAAACTGGAGCCTGAAGAGTTTGCGGCTTACCTGGAACCCTTCAAGGAGAAGGGAGA 5880
CGCGTTTGACCTCGGACTTCTCAAACGCCGAATGGACCTTGGGAAGTTCCTCTTCCCTCT

5881 GGTGAGGAGACCGACCCTGTCATGGCCTCGGGAAATTCCGCTGGTCAAAGGAGGGAAGCC 5940
CCACTCCTCTGGCTGGGACAGTACCGGAGCCCTTTAAGGCGACCAGTTTCCTCCCTTCGG

5941 AGACGTCGTCGCCATTGTCCGGAATTACAACGCTTACCTCCGCGCTAGTGACGACCTGCC 6000
TCTGCAGCAGCGGTAACAGGCCTTAATGTTGCGAATGGAGGCGCGATCACTGCTGGACGG

6001 TAAACTCTTCATCGAATCAGATCCTGGTTTCTTTAGTAACGCCATCGTCGAGGGCGCCAA 6060
ATTTGAGAAGTAGCTTAGTCTAGGACCAAAGAAATCATTGCGGTAGCAGCTCCCGCGGTT

6061 GAAGTTTCCAAACACCGAATTTGTTAAAGTCAAAGGACTTCACTTCCTCCAGGAGGATGC 6120
CTTCAAAGGTTTGTGGCTTAAACAATTTCAGTTTCCTGAAGTGAAGGAGGTCCTCCTACG

6121 GCCCGATGAAATGGGAAAGTATATCAAATCCTTCGTGGAGAGGGTCTTGAAGAATGAGCA 6180
CGGGCTACTTTACCCTTTCATATAGTTTAGGAAGCACCTCTCCCAGAACTTCTTACTCGT

6181 GAGGTCCATCgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccgg 6240
CTCCAGGTAGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCC

puro marker(6245,6844)>>> 
|
6241 ccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggc 6300
GGGATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCG

6301 cgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggaccc 6360
GCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGG

6361 ggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggct 6420
CCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGA

6421 cgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgcc 6480
GCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGG

6481 ggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgag 6540
CCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTC

6541 cggttcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaa 6600
GCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTT

6601 ggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtct 6660
CCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGA

6661 gggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcctt 6720
CCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAA

6721 cctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcac 6780
GGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTG

6781 cgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgc 6840
GCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACG

6841 ctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtat 6900
GACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATA

6901 tcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatca 6960
AGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGT

6961 tgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtc 7020
ACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAG

7021 tctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgc 7080
AGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACG

7081 tgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggacttt 7140
ACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAA

7141 cgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctg 7200
GCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGAC

7201 gacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtc 7260
CTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAG

7261 ctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgcta 7320
GAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGAT

7321 cgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcg 7380
GCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGC

7381 gcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctcc 7440
CGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGG

delta_U3 other(7454,7506)>>> 
|
7441 ccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgct 7500
GGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGA

HIV-1_5_LTR other(7507,7687)>>> 
|
7501 tgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactaggg 7560
ACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCC

7561 aacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgt 7620
TTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCA

7621 ctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatc 7680
GACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAG

7681 tctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatga 7740
AGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACT

7741 atatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaat 7800
TATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTA

7801 agcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtcc 7860
TCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGG

7861 aaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaa 7920
TTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATT

7921 tgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgt 7980
ACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACA

7981 gaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcg 8040
CTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGC

8041 ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaag 8100
GAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTC

8101 gcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaa 8160
CGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTT

pBR322 origin(8193,8812)<<< 
|
8161 ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctc 8220
CCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAG

8221 cgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgaca 8280
GCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGT

8281 ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg 8340
CCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGC

8341 accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttct 8400
TGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGA

8401 catagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt 8460
GTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACA

8461 gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgag 8520
CACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTC

8521 tccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagc 8580
AGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCG

8581 agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctac 8640
TCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATG

8641 actagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaaga 8700
TGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCT

8701 gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc 8760
CAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACG

8761 aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacg 8820
TTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGC

8821 gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca 8880
CCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGT

8881 aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt 8940
TTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCA

8941 atatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctca 9000
TATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGT

9001 gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg 9060
CGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGC

9061 atacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctca 9120
TATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGT

9121 ccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt 9180
GGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCA

9181 cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagt 9240
GGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCA

9241 agttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttt 9300
TCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAA

9301 tcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactggg 9360
AGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCC

9361 ctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggctta 9420
GATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAAT

NEOKAN prom(9465,9514)>>> 
|
9421 catggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctg 9480
GTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGAC

9481 gggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgc 9540
CCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCG

9541 caaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttc 9600
GTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAG

NTP_II marker(9606,10394)>>> 
|
ORF_4 rf(3)(9603,10397)>>> 
| |
9601 gcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctat 9660
CGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATA

9661 tcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgt 9720
AGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACA

9721 cagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaac 9780
GTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTG

9781 tgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctg 9840
ACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGAC

9841 tgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggc 9900
ACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCG

9901 aggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaa 9960
TCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTT

9961 tgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatc 10020
ACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAG

10021 gcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacg 10080
CGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGC

10081 aagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccg 10140
TTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGC

10141 acggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaa 10200
TGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTT

10201 atggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcagg 10260
TACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCC

10261 acatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgct 10320
TGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGA

10321 tcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttc 10380
AGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAG

10381 ttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaa 10440
AACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTT

10441 taggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagt 10500
ATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCA

f1 origin(10502,10807)<<< 
|
10501 gttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaaggg 10560
CAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCC

10561 cgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtttt 10620
GCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAA

10621 ttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttaga 10680
AACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCT

10681 gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcg 10740
CGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGC

10741 ggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgc 10800
CCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCG

10801 ttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaatta 10860
AATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAAT

10861 acatcatcaataatatacctt 10881
TGTAGTAGTTATTATATGGAA

                            LOCUS dna 10881 bp 
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4739
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 4077..4934
/sequence="ORF_5 rf(5)"
misc_binding 4740..4745
/dbxref="REBASE:KpnI"
misc_binding 4767..4772
/dbxref="REBASE:EcoRI"
misc_binding 4785..4790
/dbxref="REBASE:StuI"
misc_binding 4809..4816
/dbxref="REBASE:NotI"
misc_binding 4818..4823
/dbxref="REBASE:BamHI"
misc_binding 4824..4829
/dbxref="REBASE:XbaI"
misc_binding 4836..4841
/dbxref="REBASE:XhoI"
Promoter 4886..5154
/gene="SV40 prom"
Rep_Origin 5053..5130
/gene="SV40 origin"
ORF 5120..6844
/sequence="ORF_2 rf(2)"
misc_binding 5133..5145
/dbxref="REBASE:SfiI"
Marker 6245..6844
/gene="puro marker"
Other Gene 7454..7506
/gene="delta_U3 other"
Other Gene 7507..7687
/gene="HIV-1_5_LTR other"
Rep_Origin 8193..8812
/gene="pBR322 origin"
Promoter 9465..9514
/gene="NEOKAN prom"
ORF 9603..10397
/sequence="ORF_4 rf(3)"
Marker 9606..10394
/gene="NTP_II marker"
Rep_Origin 10502..10807
/gene="f1 origin"
BASE COUNT 2729 a 2735 c 2970 g 2447 t 0 others