pHSV-CMV-Neo
| Vector Name | pHSV-CMV-Neo |
| VectorType | Herpes Simplex Virus (HSV) Plasmid |
| Antibiotic Information | Bacterial: Ampicillin. Mammalian: Neomycin. |
| Sequencing Primers | CMV sequencing primer: 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer: 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Vector Size without Insert: 6055bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 gatctgcctg caggcatgca agctgtaccc ggggccggcg cggagtcggg cacggcgcca
61 gtgctcgcac ttcgccctaa taatatatat atattgggac gaagtgcgaa cgcttcgcgt
121 tctcacttct tttacccggc ggccccgccc ccttggggcg gtcccgcccg ccggccaatg
181 ggggggcggc aaggcgggcg gcccttgggc cgcccgccgt cccgttggtc ccaacgtccg
241 gcgggcggga ccgggggccc ggggatcccc ggccgcgggg ggcccgggct gcgccgccgc
301 gctttaaagg gccgcgcgcg acccccgggg ggtgtgtttt ggggggggcc cgttttcggc
361 gtctggccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
421 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
481 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctcct ccccccgctc
541 ctccccccgc tcctcccccc gctcctcccc ccgctcctcc ccccgctccc gcggccccgc
601 cccccacgcc cgccgcgcgc gcgcacgccg cccggaccgc cgcccgcctt ttttgcgcgc
661 cgccccgccc gcggggggcc cgggctgcca caggtgtaac cccgggaatt agcttggcac
721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
781 ttgcagcaca tccccctttc gccagcgatg tacgggccag atatacgcgt tgacattgat
841 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
1021 gacgtcaatg ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
1081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
1141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
1201 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
1261 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
1321 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
1381 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
1441 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
1501 ctcggatcca ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg
1561 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
1621 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
1681 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
1741 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
1801 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
1861 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
1921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
1981 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
2041 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
2101 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
2161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
2221 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
2281 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
2341 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
2401 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
2521 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
2581 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
2641 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
2701 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
2761 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
2821 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
2881 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
2941 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
3001 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
3061 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
3121 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
3181 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
3241 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
3301 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
3361 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
3421 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
3481 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
3541 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
3601 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
3661 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
3721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
3781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
3841 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
3901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
3961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
5761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
5821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
5881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cggga
LOCUS dna 6055 bp
FEATURES Location/Qualifiers
ORF 16..699
/sequence="ORF_1 rf(1)"
misc_binding 1080..1085
/dbxref="REBASE:NdeI"
Promoter 1460..1478
/gene="T7 prom"
misc_binding 1486..1491
/dbxref="REBASE:HindIII"
misc_binding 1492..1497
/dbxref="REBASE:KpnI"
misc_binding 1535..1540
/dbxref="REBASE:EcoRI"
misc_binding 1545..1550
/dbxref="REBASE:EcoRV"
misc_binding 1561..1568
/dbxref="REBASE:NotI"
misc_binding 1568..1573
/dbxref="REBASE:XhoI"
misc_binding 1580..1585
/dbxref="REBASE:XbaI"
Promoter 1597..1614
/gene="Sp6 prom"
Rep_Origin 1926..2232
/gene="f1 origin"
Regulatory_Seq 2342..2578
/gene="SV40ER reg"
Promoter 2364..2633
/gene="SV40 prom"
Promoter 2485..2686
/gene="SV40 prom"
Rep_Origin 2532..2609
/gene="SV40 origin"
misc_binding 2663..2668
/dbxref="REBASE:StuI"
ORF 2748..3542
/sequence="ORF_2 rf(3)"
Marker 2751..3539
/gene="NTP_II marker"
Promoter 3935..3964
/gene="lac prom"
Rep_Origin 4273..4892
/gene="pBR322 origin"
misc_binding 4638..4646
/dbxref="REBASE:AlwNI"
Marker 5047..5907
/gene="amp marker"
ORF 5047..5907
/sequence="ORF_3 rf(5)"
Promoter 5949..5977
/gene="amp prom"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
ORF_1 rf(1)(16,699)>>>
|
1 GATCTGCCTGCAGGCATGCAAGCTGTACCCGGGGCCGGCGCGGAGTCGGGCACGGCGCCA 60
CTAGACGGACGTCCGTACGTTCGACATGGGCCCCGGCCGCGCCTCAGCCCGTGCCGCGGT
61 GTGCTCGCACTTCGCCCTAATAATATATATATATTGGGACGAAGTGCGAACGCTTCGCGT 120
CACGAGCGTGAAGCGGGATTATTATATATATATAACCCTGCTTCACGCTTGCGAAGCGCA
121 TCTCACTTCTTTTACCCGGCGGCCCCGCCCCCTTGGGGCGGTCCCGCCCGCCGGCCAATG 180
AGAGTGAAGAAAATGGGCCGCCGGGGCGGGGGAACCCCGCCAGGGCGGGCGGCCGGTTAC
181 GGGGGGCGGCAAGGCGGGCGGCCCTTGGGCCGCCCGCCGTCCCGTTGGTCCCAACGTCCG 240
CCCCCCGCCGTTCCGCCCGCCGGGAACCCGGCGGGCGGCAGGGCAACCAGGGTTGCAGGC
241 GCGGGCGGGACCGGGGGCCCGGGGATCCCCGGCCGCGGGGGGCCCGGGCTGCGCCGCCGC 300
CGCCCGCCCTGGCCCCCGGGCCCCTAGGGGCCGGCGCCCCCCGGGCCCGACGCGGCGGCG
301 GCTTTAAAGGGCCGCGCGCGACCCCCGGGGGGTGTGTTTTGGGGGGGGCCCGTTTTCGGC 360
CGAAATTTCCCGGCGCGCGCTGGGGGCCCCCCACACAAAACCCCCCCCGGGCAAAAGCCG
361 GTCTGGCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTC 420
CAGACCGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAG
421 CTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTC 480
GAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAG
481 CTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTC 540
GAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAG
541 CTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCTCCCCCCGCTCCCGCGGCCCCGC 600
GAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGAGGGGGGCGAGGGCGCCGGGGCG
601 CCCCCACGCCCGCCGCGCGCGCGCACGCCGCCCGGACCGCCGCCCGCCTTTTTTGCGCGC 660
GGGGGTGCGGGCGGCGCGCGCGCGTGCGGCGGGCCTGGCGGCGGGCGGAAAAAACGCGCG
661 CGCCCCGCCCGCGGGGGGCCCGGGCTGCCACAGGTGTAACCCCGGGAATTAGCTTGGCAC 720
GCGGGGCGGGCGCCCCCCGGGCCCGACGGTGTCCACATTGGGGCCCTTAATCGAACCGTG
721 TGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC 780
ACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGG
781 TTGCAGCACATCCCCCTTTCGCCAGCGATGTACGGGCCAGATATACGCGTTGACATTGAT 840
AACGTCGTGTAGGGGGAAAGCGGTCGCTACATGCCCGGTCTATATGCGCAACTGTAACTA
841 TATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGG 900
ATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACC
901 AGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCC 960
TCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGG
961 GCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATT 1020
CGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAA
1021 GACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC 1080
CTGCAGTTACCCACCTGATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAG
NdeI
|
1081 ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG 1140
TATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATAC
1141 CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCG 1200
GGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGC
1201 CTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACT 1260
GATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGA
1261 CACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA 1320
GTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTT
1321 ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTA 1380
TAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCAT
1381 GGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTG 1440
CCGCACATGCCACCCTCCAGATATATTCGTCTCGAGAGACCGATTGATCTCTTGGGTGAC
T7 prom(1460,1478)>>> HindIII KpnI
| | |
1441 CTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGTACCGAG 1500
GAATGACCGAATAGCTTTAATTATGCTGAGTGATATCCCTCTGGGTTCGAACCATGGCTC
EcoRI EcoRV
| |
1501 CTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACACTG 1560
GAGCCTAGGTGATCATTGCCGGCGGTCACACGACCTTAAGACGTCTATAGGTAGTGTGAC
NotI XhoI XbaI Sp6 prom(1597,1614)<<<
| | | |
1561 GCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAG 1620
CGCCGGCGAGCTCGTACGTAGATCTCCCGGGATAAGATATCACAGTGGATTTACGATCTC
1621 CTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC 1680
GAGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGG
1681 CCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGG 1740
GGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCC
1741 AAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGG 1800
TTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCC
1801 ACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTA 1860
TGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCCACCCGAGAT
1861 TGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTA 1920
ACCGAAGACTCCGCCTTTCTTGGTCGACCCCGAGATCCCCCATAGGGGTGCGCGGGACAT
f1 origin(1926,2232)>>>
|
1921 GCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCA 1980
CGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGT
1981 GCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCT 2040
CGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGA
2041 TTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGC 2100
AAGGGGCAGTTCGAGATTTAGCCCCGTAGGGAAATCCCAAGGCTAAATCACGAAATGCCG
2101 ACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGAT 2160
TGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTA
2161 AGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCC 2220
TCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGG
2221 AAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGG 2280
TTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACC
2281 GGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAAT 2340
CCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAATTA
SV40 prom(2364,2633)>>>
|
SV40ER reg(2342,2578)<<<
| |
2341 TCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGGCAGGCAGAA 2400
AGACACCTTACACACAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCCGTCCGTCTT
2401 GTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCC 2460
CATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGG
SV40 prom(2485,2686)>>>
|
2461 CAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCC 2520
GTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGG
SV40 origin(2532,2609)>>>
|
2521 TAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCT 2580
ATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGA
2581 GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGA 2640
CTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGACGGAGACTCGATAAGGTCT
StuI
|
2641 AGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTA 2700
TCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTTCGAGGGCCCTCGAACAT
NTP_II marker(2751,3539)>>>
|
ORF_2 rf(3)(2748,3542)>>>
| |
2701 TATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAG 2760
ATAGGTAAAAGCCTAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTC
2761 ATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGG 2820
TACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCC
2821 CACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCC 2880
GTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGG
2881 CGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAG 2940
GCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTC
2941 CGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCA 3000
GCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGT
3001 CTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCAT 3060
GACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTA
3061 CTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATA 3120
GAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTAT
3121 CGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCAC 3180
GCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTG
3181 GTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGC 3240
CATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCG
3241 TCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCG 3300
AGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGC
3301 TCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTG 3360
AGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGAC
3361 GATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTA 3420
CTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGAT
3421 CCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACG 3480
GGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGC
3481 GTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCT 3540
CATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGA
3541 GAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGA 3600
CTCGCCCTGAGACCCCAAGCTTTACTGGCTGGTTCGCTGCGGGTTGGACGGTAGTGCTCT
3601 TTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGC 3660
AAAGCTAAGGTGGCGGCGGAAGATACTTTCCAACCCGAAGCCTTAGCAAAAGGCCCTGCG
3661 CGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTT 3720
GCCGACCTACTAGGAGGTCGCGCCCCTAGAGTACGACCTCAAGAAGCGGGTGGGGTTGAA
3721 GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA 3780
CAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATT
3781 AGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCA 3840
TCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGT
3841 TGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCC 3900
ACAGACATATGGCAGCTGGAGATCGATCTCGAACCGCATTAGTACCAGTATCGACAAAGG
lac prom(3935,3964)<<<
|
3901 TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTG 3960
ACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCAC
3961 TAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC 4020
ATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGG
4021 CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG 4080
GCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCC
4081 GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC 4140
CTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAG
4141 GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC 4200
CCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTG
4201 AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA 4260
TCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTT
pBR322 origin(4273,4892)<<<
|
4261 CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA 4320
GGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGT
4321 CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC 4380
GTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCG
4381 GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA 4440
CAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTAT
4441 CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTA 4500
GGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTTACGAGTGCGACATCCAT
4501 TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA 4560
AGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGT
4561 GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA 4620
CGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCT
AlwNI
|
4621 CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG 4680
GAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCC
4681 TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG 4740
ACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACC
4741 TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG 4800
ATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCC
4801 CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG 4860
GTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTC
4861 AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA 4920
TTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTT
4921 CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT 4980
GCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTA
4981 CCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTC 5040
GGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAG
ORF_3 rf(5)(5047,5907)<<<
|
amp marker(5047,5907)<<<
|
5041 TGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTC 5100
ACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAG
5101 ATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATC 5160
TAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAG
5161 TGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGC 5220
ACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCG
5221 AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC 5280
TTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAG
5281 CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT 5340
GTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAA
5341 GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGC 5400
CGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCG
5401 TTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAA 5460
AAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTT
5461 AAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTT 5520
TTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAA
5521 ATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATG 5580
TAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTAC
5581 CTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACC 5640
GAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGG
5641 GAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA 5700
CTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTT
5701 AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTT 5760
TCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAA
5761 GAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTT 5820
CTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAA
5821 CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAG 5880
GTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTC
5881 GGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTA 5940
CCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAAT
amp prom(5949,5977)<<<
|
5941 TCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAT 6000
AGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTA
6001 AGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGA 6055
TCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGCTGCCTAGCCCT