pAdeno-hTERT Antisense

Vector Name	pAdeno-hTERT Antisense
VectorType	Retroviral Vector
Antibiotic Information	No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds.
Sequencing Primers	Sequencing primer: (1311-13300) 5`---GATAGAGATCGTCGACGAGC ---3`
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 360
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 420
421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg 480
481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 540
541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 600
601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 660
661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 720
721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 780
781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 840
841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 900
901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 960
961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 1020
1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 1080
1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 1140
1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa 1200
1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 1260
1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc 1320
1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 1380
1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc 1440
1441 agtgtggtgg aattcccacc tcggctcgcg gtcggacacc ccttcacttc tgccgtccac 1500
1501 acgacctgtg agtcgggaac cgacctgtga gcgagtccgg agtcggcctg tgagtcggaa 1560
1561 gtcggcctgt acgtccggag ccggtttgtg agtgagtccg gagtctgagg gtcgccacgc 1620
1621 ccggacccac acccggcggg gagggaggga ccctgcatct cgggccgcac tgtcccgacg 1680
1681 accacagacg agagccggac cgacacccgc ccaccggtag tcaggtccta ccagaacttc 1740
1741 agactcccgt cacggcccaa ccgacgccgg aggtcccgtc agtcgcagca ggggccctcg 1800
1801 aaggctgagt cgacgcagac ccgacaggac tcactggggt cctcaccgtg catccactgt 1860
1861 gccacagctc agtcgaactc gtccttacga accaccgtgt cggtgacgtg ccggagcctc 1920
1921 ccgtctcccg gccgccgcgg gaaccggggg tcgctgtagg gacgcaagaa ccgaaagtcc 1980
1981 tacctcatcg tctccctccg gcacagtctc tactgcgcgt cctttttaca ccccaagaag 2040
2041 gtttgaacga ctactttacc ctcgacgtcg tgtgtacgca ctttggacat gcggacgtcg 2100
2101 tcctcctaga acatctacaa ccacgtgtgg cagacctccg acaagtggac gtttaggtct 2160
2161 ttgtccgaca ctgtgaagtc ggcgttctgg ggtttctcaa acgctgcgta caaggagggt 2220
2221 cggaacttcg gcgccaactt ccactctgac cgagactacc tccaggcccg tatcgacctc 2280
2281 atcagcgaga cgtggaggtc ccaggcccat aggtcgtcgt ccggcgtggt ccccttatcc 2340
2341 ggcacccggc cgtagacttg ttttcggcac ggtgggtccc ggagcagaag atgtcccttc 2400
2401 aagtggtgac agaaggcgtt caagtggtgc gtcggtatga gtccctgtgg agcctggtcc 2460
2461 caggactcct tccaaaagcg cacccactcc actccacagt ggttgttctt tagtaggtgg 2520
2521 tttgcgtcct cgtcgggcag ggcggcttag gggcgtttgt cgaacaagag gtacagcggc 2580
2581 atcgtgtccg acgtctcgtc gcacctctcc tacctcggga cgccctaggg gaccgtgacc 2640
2641 tgcatcctga acggggacta cgcgtgccgc accaccgtgt acttcgcatc cttctgcagc 2700
2701 ttctccggtg acgaccggag taagtccctc ctcgagacga gctactgctg ccgtagggag 2760
2761 tcgcccgacc agaggacgtc cactcggtgc ttgacagcgt acatgccgac ctccagacag 2820
2821 ttccatctct gcaccgagaa cttccggaac gcctgcacgg gtacccgccg gaagacctgg 2880
2881 tgccgtatgg ctgcgtgcgt catgcacaag accccaaact actacgaccg ctactggagg 2940
2941 cactcggaca ggacccccta ccacagcatg cgcgggcagt gtaggtggaa ctgtttcatg 3000
3001 tcgagtccgc cgcccaggac ccgggcgtgt gcgtcgtgct tccacgcggt ccgggacacc 3060
3061 tatagcaggt ccgggtcgtg tctccgcggg tcctccggcc ccgcggcgcg ggcgagcatc 3120
3121 aactcgtgcg acttgtcacg gaagtgggag ctccactctg cgagccggga gaaaagagac 3180
3181 gccttgcaag accgagggtg ctgcatcagg tacaagtgtt agccggcgtc gggcagtccg 3240
3241 aacccctact tcgcctcaga cctgcagtcg tcccgcccgg accgaagggc tacgacggac 3300
3301 tggagacgaa ggctgtcgag ggcgtcgacg tgggagaagt tcacgacaga ctaaggttac 3360
3361 gaaacgttga acgaggtctg tgagaaggcc atctttttct cggacaagaa aactttgcac 3420
3421 cagaggcact gtattttctt tctggactcg tcgagctgct gcatgtgtga gtagtcggtc 3480
3481 acgtccttga accggtccta gaggagtgcg tctgccacga gacgccggcc ttgtgtcggt 3540
3541 tggggacccg aggacgcgtc ggttcgcgtc agggcgtgcg agtagaaggt gcagtcgagg 3600
3601 acgtcgctct cgaaccgtac gaaggggtcc ctctacttga agaaccacaa ggactccttc 3660
3661 gccgcaagca acacggacct cggggtctcc ggacccccgt ggtcggccgc gtccgtccgg 3720
3721 gcgtgcttcg gcatgtggac ggtccccgac gacacgaccg cctcgtcgac gtggtccgct 3780
3781 gcccccagac acaggaggag gagcccccgg cggtgtctcg ggaccccgaa gagggcccgt 3840
3841 gtctgtggcc gacgacccca ctggcgtcga gcgtcgcccg tcacgcagaa ctcctcgtgg 3900
3901 ggcatccccg tgacgcgcac caagggttcg tcgaggtctt tgtccccggc gtaaacggtc 3960
3961 atcgcgaccc cgtccgcccc gttggacgcc cctcagggac cgtaggtccc ggaccttggg 4020
4021 tctttctacc agaggtgctc ggaggctcgc ggtcagtccg acccggagtc tctcgactca 4080
4081 tccttcctcc cggcgtcgac gaggaacagc ggactcctca tctccttcac gaaccagagc 4140
4141 cgcatgtggc cccctgttcc gcacagggtc cctgcaccac cggcgctaca cctacccccc 4200
4201 gggcgcacca cgaccgccgg gtgcctaccc accctcaccg cgcacggtct ctcgcgtggg 4260
4261 aggtttctcc accgaagaag ccgcccagac cgtccactgt ggtgtgtctt tggtgccagt 4320
4321 gagccaggtg cgcaggacgg gcccacccgg gtcctgggga cgggttgccc gcaggcgagg 4380
4381 ccgagtcccc gtcgcggtgc ggacccggag aacccgttgc cgtctgaagc cgaccgtgac 4440
4441 gggggcgcgg aggagcgtgg gccccgaccg tccgggtccc cctggggccg gagggactgc 4500
4501 gataccaagg tccgggcaag cgtagggtct gcggaagccc caggtgatcg cacaccgccc 4560
4561 ccggcccgga ctcaccgtcg cggctcgacc atgtcgccgc cgggcgtgtg gaccatccgc 4620
4621 gtcgaccctc ggtggtcgtg tttctcgcgc gtcgcacggt cgtccacttg gtcgtgcagc 4680
4681 agcgggtgcg ccgcgtcgtc gtcgggggtg cggggcgagg gggcgtcacg cagccagtgg 4740
4741 cacaacccgt ccatcgacgc gtgcgaccac cacttccgga gcccccccgg gggcgcccgg 4800
4801 ggcaggtcgt cgcgcttcgg cttccggtcg tgcaagaagc gcggcgcgag cgtgtcggag 4860
4861 acgtcgtgag cccggtggtc gaggaagtcc gtcctgtgga ccgccttcct cccccgccgc 4920
4921 cccccgccgg cacgcagggt cccgtgcgtg tggtccgtga cccggtggtc gcgcgccttt 4980
4981 cggcggccca ggggcgcgac gtggtcggcg gtcgggaccc cggggtccgc ggcgtgcttg 5040
5041 caccggtcgc cgtcgtggag cgccatcacc gacgcgtcgt ccctcgcgtg ccgagccgtc 5100
5101 gcccctcgcg cgccgtagcg cccccaccgg ccccggtccc gaagggtgca cgcgtcgtcc 5160
5161 tgcgtcgcga cggacccctg cagatatcca gcacagtggc ggccgctcga gtctagaggg 5220
5221 cccgtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 5280
5281 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 5340
5341 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 5400
5401 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 5460
5461 tgggctctat ggcttctgag gcggaaagaa ccagcagatc gatctgctta tgggtattat 5520
5521 gggtctgcat taatgaatcg gccaacggat cccggtgtga aataccgcac agatgcgtaa 5580
5581 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5640
5641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5700
5701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5760
5761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5820
5821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5880
5881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5940
5941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6000
6001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6060
6061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6120
6121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6180
6181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6240
6241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6300
6301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6360
6361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6420
6421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6480
6481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6540
6541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6600
6601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6660
6661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6720
6721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6780
6781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6840
6841 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca 6900
6901 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc 6960
6961 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 7020
7021 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg 7080
7081 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg 7140
7141 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 7200
7201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 7260
7261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 7320
7321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 7380
7381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 7440
7441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 7500
7501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 7560
7561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 7620
7621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 7680
7681 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 7740
7741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 7800
7801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 7860
7861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 7920
7921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt 7980
7981 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc 8040
8041 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga 8100
8101 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 8160
8161 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag 8220
8221 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 8280
8281 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 8340
8341 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc 8400
8401 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt 8459

                            LOCUS dna 8459 bp 
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 942..947
/dbxref="REBASE:NdeI"
Regulatory_Seq 1279..1318
/gene="tetO reg"
misc_binding 1409..1414
/dbxref="REBASE:HindIII"
misc_binding 1450..1455
/dbxref="REBASE:EcoRI"
promoter 1456..5170
/gene="hTERT"
ORF 4144..4869
/sequence="ORF_1 rf(1)"
misc_binding 5183..5188
/dbxref="REBASE:EcoRV"
misc_binding 5199..5206
/dbxref="REBASE:NotI"
misc_binding 5212..5217
/dbxref="REBASE:XbaI"
misc_binding 5498..5503
/dbxref="REBASE:ClaI"
selectable_marker661..6441
/gene="Kan/Neo"
Rep_Origin 5771..6390
/gene="pBR322 origin"
Promoter 7043..7092
/gene="NEOKAN prom"
ORF 7181..7975
/sequence="ORF_2 rf(2)"
Marker 7184..7972
/gene="NTP_II marker"
Rep_Origin 8080..8385
/gene="f1 origin"
BASE COUNT 1748 a 2434 c 2491 g 1786 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg 
481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 
541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 
601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 
661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 
721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 
781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 
841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 
901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 
961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 
1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 
1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 
1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa 
1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 
1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc 
1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 
1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc 
1441 agtgtggtgg aattcccacc tcggctcgcg gtcggacacc ccttcacttc tgccgtccac 
1501 acgacctgtg agtcgggaac cgacctgtga gcgagtccgg agtcggcctg tgagtcggaa 
1561 gtcggcctgt acgtccggag ccggtttgtg agtgagtccg gagtctgagg gtcgccacgc 
1621 ccggacccac acccggcggg gagggaggga ccctgcatct cgggccgcac tgtcccgacg 
1681 accacagacg agagccggac cgacacccgc ccaccggtag tcaggtccta ccagaacttc 
1741 agactcccgt cacggcccaa ccgacgccgg aggtcccgtc agtcgcagca ggggccctcg 
1801 aaggctgagt cgacgcagac ccgacaggac tcactggggt cctcaccgtg catccactgt 
1861 gccacagctc agtcgaactc gtccttacga accaccgtgt cggtgacgtg ccggagcctc 
1921 ccgtctcccg gccgccgcgg gaaccggggg tcgctgtagg gacgcaagaa ccgaaagtcc 
1981 tacctcatcg tctccctccg gcacagtctc tactgcgcgt cctttttaca ccccaagaag 
2041 gtttgaacga ctactttacc ctcgacgtcg tgtgtacgca ctttggacat gcggacgtcg 
2101 tcctcctaga acatctacaa ccacgtgtgg cagacctccg acaagtggac gtttaggtct 
2161 ttgtccgaca ctgtgaagtc ggcgttctgg ggtttctcaa acgctgcgta caaggagggt 
2221 cggaacttcg gcgccaactt ccactctgac cgagactacc tccaggcccg tatcgacctc 
2281 atcagcgaga cgtggaggtc ccaggcccat aggtcgtcgt ccggcgtggt ccccttatcc 
2341 ggcacccggc cgtagacttg ttttcggcac ggtgggtccc ggagcagaag atgtcccttc 
2401 aagtggtgac agaaggcgtt caagtggtgc gtcggtatga gtccctgtgg agcctggtcc 
2461 caggactcct tccaaaagcg cacccactcc actccacagt ggttgttctt tagtaggtgg 
2521 tttgcgtcct cgtcgggcag ggcggcttag gggcgtttgt cgaacaagag gtacagcggc 
2581 atcgtgtccg acgtctcgtc gcacctctcc tacctcggga cgccctaggg gaccgtgacc 
2641 tgcatcctga acggggacta cgcgtgccgc accaccgtgt acttcgcatc cttctgcagc 
2701 ttctccggtg acgaccggag taagtccctc ctcgagacga gctactgctg ccgtagggag 
2761 tcgcccgacc agaggacgtc cactcggtgc ttgacagcgt acatgccgac ctccagacag 
2821 ttccatctct gcaccgagaa cttccggaac gcctgcacgg gtacccgccg gaagacctgg 
2881 tgccgtatgg ctgcgtgcgt catgcacaag accccaaact actacgaccg ctactggagg 
2941 cactcggaca ggacccccta ccacagcatg cgcgggcagt gtaggtggaa ctgtttcatg 
3001 tcgagtccgc cgcccaggac ccgggcgtgt gcgtcgtgct tccacgcggt ccgggacacc 
3061 tatagcaggt ccgggtcgtg tctccgcggg tcctccggcc ccgcggcgcg ggcgagcatc 
3121 aactcgtgcg acttgtcacg gaagtgggag ctccactctg cgagccggga gaaaagagac 
3181 gccttgcaag accgagggtg ctgcatcagg tacaagtgtt agccggcgtc gggcagtccg 
3241 aacccctact tcgcctcaga cctgcagtcg tcccgcccgg accgaagggc tacgacggac 
3301 tggagacgaa ggctgtcgag ggcgtcgacg tgggagaagt tcacgacaga ctaaggttac 
3361 gaaacgttga acgaggtctg tgagaaggcc atctttttct cggacaagaa aactttgcac 
3421 cagaggcact gtattttctt tctggactcg tcgagctgct gcatgtgtga gtagtcggtc 
3481 acgtccttga accggtccta gaggagtgcg tctgccacga gacgccggcc ttgtgtcggt 
3541 tggggacccg aggacgcgtc ggttcgcgtc agggcgtgcg agtagaaggt gcagtcgagg 
3601 acgtcgctct cgaaccgtac gaaggggtcc ctctacttga agaaccacaa ggactccttc 
3661 gccgcaagca acacggacct cggggtctcc ggacccccgt ggtcggccgc gtccgtccgg 
3721 gcgtgcttcg gcatgtggac ggtccccgac gacacgaccg cctcgtcgac gtggtccgct 
3781 gcccccagac acaggaggag gagcccccgg cggtgtctcg ggaccccgaa gagggcccgt 
3841 gtctgtggcc gacgacccca ctggcgtcga gcgtcgcccg tcacgcagaa ctcctcgtgg 
3901 ggcatccccg tgacgcgcac caagggttcg tcgaggtctt tgtccccggc gtaaacggtc 
3961 atcgcgaccc cgtccgcccc gttggacgcc cctcagggac cgtaggtccc ggaccttggg 
4021 tctttctacc agaggtgctc ggaggctcgc ggtcagtccg acccggagtc tctcgactca 
4081 tccttcctcc cggcgtcgac gaggaacagc ggactcctca tctccttcac gaaccagagc 
4141 cgcatgtggc cccctgttcc gcacagggtc cctgcaccac cggcgctaca cctacccccc 
4201 gggcgcacca cgaccgccgg gtgcctaccc accctcaccg cgcacggtct ctcgcgtggg 
4261 aggtttctcc accgaagaag ccgcccagac cgtccactgt ggtgtgtctt tggtgccagt 
4321 gagccaggtg cgcaggacgg gcccacccgg gtcctgggga cgggttgccc gcaggcgagg 
4381 ccgagtcccc gtcgcggtgc ggacccggag aacccgttgc cgtctgaagc cgaccgtgac 
4441 gggggcgcgg aggagcgtgg gccccgaccg tccgggtccc cctggggccg gagggactgc 
4501 gataccaagg tccgggcaag cgtagggtct gcggaagccc caggtgatcg cacaccgccc 
4561 ccggcccgga ctcaccgtcg cggctcgacc atgtcgccgc cgggcgtgtg gaccatccgc 
4621 gtcgaccctc ggtggtcgtg tttctcgcgc gtcgcacggt cgtccacttg gtcgtgcagc 
4681 agcgggtgcg ccgcgtcgtc gtcgggggtg cggggcgagg gggcgtcacg cagccagtgg 
4741 cacaacccgt ccatcgacgc gtgcgaccac cacttccgga gcccccccgg gggcgcccgg 
4801 ggcaggtcgt cgcgcttcgg cttccggtcg tgcaagaagc gcggcgcgag cgtgtcggag 
4861 acgtcgtgag cccggtggtc gaggaagtcc gtcctgtgga ccgccttcct cccccgccgc 
4921 cccccgccgg cacgcagggt cccgtgcgtg tggtccgtga cccggtggtc gcgcgccttt 
4981 cggcggccca ggggcgcgac gtggtcggcg gtcgggaccc cggggtccgc ggcgtgcttg 
5041 caccggtcgc cgtcgtggag cgccatcacc gacgcgtcgt ccctcgcgtg ccgagccgtc 
5101 gcccctcgcg cgccgtagcg cccccaccgg ccccggtccc gaagggtgca cgcgtcgtcc 
5161 tgcgtcgcga cggacccctg cagatatcca gcacagtggc ggccgctcga gtctagaggg 
5221 cccgtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
5281 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
5341 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
5401 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
5461 tgggctctat ggcttctgag gcggaaagaa ccagcagatc gatctgctta tgggtattat 
5521 gggtctgcat taatgaatcg gccaacggat cccggtgtga aataccgcac agatgcgtaa 
5581 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
5641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
5701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
5761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
5821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
5881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
5941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
6001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
6061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
6121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
6181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
6241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
6301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
6361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
6421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
6481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
6541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
6601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
6661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
6721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
6781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
6841 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca 
6901 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc 
6961 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 
7021 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg 
7081 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg 
7141 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
7201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
7261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
7321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 
7381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
7441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
7501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
7561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
7621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
7681 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 
7741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
7801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
7861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
7921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt 
7981 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc 
8041 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga 
8101 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 
8161 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag 
8221 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 
8281 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 
8341 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc 
8401 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt 
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

Encap other(162,310)>>> 
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

amp prom(365,393)<<< 
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

421 gttccgcgcacatttccccgaaaagtgccacctgacgtaagctcagatctggatctcccg 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTCGAGTCTAGACCTAGAGGGC

481 atcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatc 540
TAGGGGATACCAGCTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATAG

541 tgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaa 600
ACGAGGGACGAACACACAACCTCCAGCGACTCATCACGCGCTCGTTTTAAATTCGATGTT

601 caaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgc 660
GTTCCGTTCCGAACTGGCTGTTAACGTACTTCTTAGACGAATCCCAATCCGCAAAACGCG

661 tgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaat 720
ACGAAGCGCTACATGCCCGGTCTATATGCGCAACTGTAACTAATAACTGATCAATAATTA

721 agtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataac 780
TCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTG

781 ttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataa 840
AATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATT

841 tgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagt 900
ACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCA

NdeI 
|
901 atttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgcccc 960
TAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGG

961 ctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttat 1020
GATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATA

1021 gggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgc 1080
CCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACG

1081 ggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc 1140
CCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAG

1141 tccaccccattgacgtcaatgggagtttgttttggaaccaaaatcaacgggactttccaa 1200
AGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCTTGGTTTTAGTTGCCCTGAAAGGTT

1201 aatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggagg 1260
TTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCC

tetO reg(1279,1318)>>> 
|
1261 tctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatc 1320
AGATATATTCGTCTCGAGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTAG

1321 gtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttg 1380
CAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAAC

HindIII 
|
1381 acctccatagaagctagcgtttaaacttaagcttggtaccgagctcggatccactagtcc 1440
TGGAGGTATCTTCGATCGCAAATTTGAATTCGAACCATGGCTCGAGCCTAGGTGATCAGG

hTERT(1456,5170)>>> 
|
EcoRI 
| |
1441 agtgtggtggaattcccacctcggctcgcggtcggacaccccttcacttctgccgtccac 1500
TCACACCACCTTAAGGGTGGAGCCGAGCGCCAGCCTGTGGGGAAGTGAAGACGGCAGGTG

1501 acgacctgtgagtcgggaaccgacctgtgagcgagtccggagtcggcctgtgagtcggaa 1560
TGCTGGACACTCAGCCCTTGGCTGGACACTCGCTCAGGCCTCAGCCGGACACTCAGCCTT

1561 gtcggcctgtacgtccggagccggtttgtgagtgagtccggagtctgagggtcgccacgc 1620
CAGCCGGACATGCAGGCCTCGGCCAAACACTCACTCAGGCCTCAGACTCCCAGCGGTGCG

1621 ccggacccacacccggcggggagggagggaccctgcatctcgggccgcactgtcccgacg 1680
GGCCTGGGTGTGGGCCGCCCCTCCCTCCCTGGGACGTAGAGCCCGGCGTGACAGGGCTGC

1681 accacagacgagagccggaccgacacccgcccaccggtagtcaggtcctaccagaacttc 1740
TGGTGTCTGCTCTCGGCCTGGCTGTGGGCGGGTGGCCATCAGTCCAGGATGGTCTTGAAG

1741 agactcccgtcacggcccaaccgacgccggaggtcccgtcagtcgcagcaggggccctcg 1800
TCTGAGGGCAGTGCCGGGTTGGCTGCGGCCTCCAGGGCAGTCAGCGTCGTCCCCGGGAGC

1801 aaggctgagtcgacgcagacccgacaggactcactggggtcctcaccgtgcatccactgt 1860
TTCCGACTCAGCTGCGTCTGGGCTGTCCTGAGTGACCCCAGGAGTGGCACGTAGGTGACA

1861 gccacagctcagtcgaactcgtccttacgaaccaccgtgtcggtgacgtgccggagcctc 1920
CGGTGTCGAGTCAGCTTGAGCAGGAATGCTTGGTGGCACAGCCACTGCACGGCCTCGGAG

1921 ccgtctcccggccgccgcgggaaccgggggtcgctgtagggacgcaagaaccgaaagtcc 1980
GGCAGAGGGCCGGCGGCGCCCTTGGCCCCCAGCGACATCCCTGCGTTCTTGGCTTTCAGG

1981 tacctcatcgtctccctccggcacagtctctactgcgcgtcctttttacaccccaagaag 2040
ATGGAGTAGCAGAGGGAGGCCGTGTCAGAGATGACGCGCAGGAAAAATGTGGGGTTCTTC

2041 gtttgaacgactactttaccctcgacgtcgtgtgtacgcactttggacatgcggacgtcg 2100
CAAACTTGCTGATGAAATGGGAGCTGCAGCACACATGCGTGAAACCTGTACGCCTGCAGC

2101 tcctcctagaacatctacaaccacgtgtggcagacctccgacaagtggacgtttaggtct 2160
AGGAGGATCTTGTAGATGTTGGTGCACACCGTCTGGAGGCTGTTCACCTGCAAATCCAGA

2161 ttgtccgacactgtgaagtcggcgttctggggtttctcaaacgctgcgtacaaggagggt 2220
AACAGGCTGTGACACTTCAGCCGCAAGACCCCAAAGAGTTTGCGACGCATGTTCCTCCCA

2221 cggaacttcggcgccaacttccactctgaccgagactacctccaggcccgtatcgacctc 2280
GCCTTGAAGCCGCGGTTGAAGGTGAGACTGGCTCTGATGGAGGTCCGGGCATAGCTGGAG

2281 atcagcgagacgtggaggtcccaggcccataggtcgtcgtccggcgtggtccccttatcc 2340
TAGTCGCTCTGCACCTCCAGGGTCCGGGTATCCAGCAGCAGGCCGCACCAGGGGAATAGG

2341 ggcacccggccgtagacttgttttcggcacggtgggtcccggagcagaagatgtcccttc 2400
CCGTGGGCCGGCATCTGAACAAAAGCCGTGCCACCCAGGGCCTCGTCTTCTACAGGGAAG

2401 aagtggtgacagaaggcgttcaagtggtgcgtcggtatgagtccctgtggagcctggtcc 2460
TTCACCACTGTCTTCCGCAAGTTCACCACGCAGCCATACTCAGGGACACCTCGGACCAGG

2461 caggactccttccaaaagcgcacccactccactccacagtggttgttctttagtaggtgg 2520
GTCCTGAGGAAGGTTTTCGCGTGGGTGAGGTGAGGTGTCACCAACAAGAAATCATCCACC

2521 tttgcgtcctcgtcgggcagggcggcttaggggcgtttgtcgaacaagaggtacagcggc 2580
AAACGCAGGAGCAGCCCGTCCCGCCGAATCCCCGCAAACAGCTTGTTCTCCATGTCGCCG

2581 atcgtgtccgacgtctcgtcgcacctctcctacctcgggacgccctaggggaccgtgacc 2640
TAGCACAGGCTGCAGAGCAGCGTGGAGAGGATGGAGCCCTGCGGGATCCCCTGGCACTGG

2641 tgcatcctgaacggggactacgcgtgccgcaccaccgtgtacttcgcatccttctgcagc 2700
ACGTAGGACTTGCCCCTGATGCGCACGGCGTGGTGGCACATGAAGCGTAGGAAGACGTCG

2701 ttctccggtgacgaccggagtaagtccctcctcgagacgagctactgctgccgtagggag 2760
AAGAGGCCACTGCTGGCCTCATTCAGGGAGGAGCTCTGCTCGATGACGACGGCATCCCTC

2761 tcgcccgaccagaggacgtccactcggtgcttgacagcgtacatgccgacctccagacag 2820
AGCGGGCTGGTCTCCTGCAGGTGAGCCACGAACTGTCGCATGTACGGCTGGAGGTCTGTC

2821 ttccatctctgcaccgagaacttccggaacgcctgcacgggtacccgccggaagacctgg 2880
AAGGTAGAGACGTGGCTCTTGAAGGCCTTGCGGACGTGCCCATGGGCGGCCTTCTGGACC

2881 tgccgtatggctgcgtgcgtcatgcacaagaccccaaactactacgaccgctactggagg 2940
ACGGCATACCGACGCACGCAGTACGTGTTCTGGGGTTTGATGATGCTGGCGATGACCTCC

2941 cactcggacaggaccccctaccacagcatgcgcgggcagtgtaggtggaactgtttcatg 3000
GTGAGCCTGTCCTGGGGGATGGTGTCGTACGCGCCCGTCACATCCACCTTGACAAAGTAC

3001 tcgagtccgccgcccaggacccgggcgtgtgcgtcgtgcttccacgcggtccgggacacc 3060
AGCTCAGGCGGCGGGTCCTGGGCCCGCACACGCAGCACGAAGGTGCGCCAGGCCCTGTGG

3061 tatagcaggtccgggtcgtgtctccgcgggtcctccggccccgcggcgcgggcgagcatc 3120
ATATCGTCCAGGCCCAGCACAGAGGCGCCCAGGAGGCCGGGGCGCCGCGCCCGCTCGTAG

3121 aactcgtgcgacttgtcacggaagtgggagctccactctgcgagccgggagaaaagagac 3180
TTGAGCACGCTGAACAGTGCCTTCACCCTCGAGGTGAGACGCTCGGCCCTCTTTTCTCTG

3181 gccttgcaagaccgagggtgctgcatcaggtacaagtgttagccggcgtcgggcagtccg 3240
CGGAACGTTCTGGCTCCCACGACGTAGTCCATGTTCACAATCGGCCGCAGCCCGTCAGGC

3241 aacccctacttcgcctcagacctgcagtcgtcccgcccggaccgaagggctacgacggac 3300
TTGGGGATGAAGCGGAGTCTGGACGTCAGCAGGGCGGGCCTGGCTTCCCGATGCTGCCTG

3301 tggagacgaaggctgtcgagggcgtcgacgtgggagaagttcacgacagactaaggttac 3360
ACCTCTGCTTCCGACAGCTCCCGCAGCTGCACCCTCTTCAAGTGCTGTCTGATTCCAATG

3361 gaaacgttgaacgaggtctgtgagaaggccatctttttctcggacaagaaaactttgcac 3420
CTTTGCAACTTGCTCCAGACACTCTTCCGGTAGAAAAAGAGCCTGTTCTTTTGAAACGTG

3421 cagaggcactgtattttctttctggactcgtcgagctgctgcatgtgtgagtagtcggtc 3480
GTCTCCGTGACATAAAAGAAAGACCTGAGCAGCTCGACGACGTACACACTCATCAGCCAG

3481 acgtccttgaaccggtcctagaggagtgcgtctgccacgagacgccggccttgtgtcggt 3540
TGCAGGAACTTGGCCAGGATCTCCTCACGCAGACGGTGCTCTGCGGCCGGAACACAGCCA

3541 tggggacccgaggacgcgtcggttcgcgtcagggcgtgcgagtagaaggtgcagtcgagg 3600
ACCCCTGGGCTCCTGCGCAGCCAAGCGCAGTCCCGCACGCTCATCTTCCACGTCAGCTCC

3601 acgtcgctctcgaaccgtacgaaggggtccctctacttgaagaaccacaaggactccttc 3660
TGCAGCGAGAGCTTGGCATGCTTCCCCAGGGAGATGAACTTCTTGGTGTTCCTGAGGAAG

3661 gccgcaagcaacacggacctcggggtctccggacccccgtggtcggccgcgtccgtccgg 3720
CGGCGTTCGTTGTGCCTGGAGCCCCAGAGGCCTGGGGGCACCAGCCGGCGCAGGCAGGCC

3721 gcgtgcttcggcatgtggacggtccccgacgacacgaccgcctcgtcgacgtggtccgct 3780
CGCACGAAGCCGTACACCTGCCAGGGGCTGCTGTGCTGGCGGAGCAGCTGCACCAGGCGA

3781 gcccccagacacaggaggaggagcccccggcggtgtctcgggaccccgaagagggcccgt 3840
CGGGGGTCTGTGTCCTCCTCCTCGGGGGCCGCCACAGAGCCCTGGGGCTTCTCCCGGGCA

3841 gtctgtggccgacgaccccactggcgtcgagcgtcgcccgtcacgcagaactcctcgtgg 3900
CAGACACCGGCTGCTGGGGTGACCGCAGCTCGCAGCGGGCAGTGCGTCTTGAGGAGCACC

3901 ggcatccccgtgacgcgcaccaagggttcgtcgaggtctttgtccccggcgtaaacggtc 3960
CCGTAGGGGCACTGCGCGTGGTTCCCAAGCAGCTCCAGAAACAGGGGCCGCATTTGCCAG

3961 atcgcgaccccgtccgccccgttggacgcccctcagggaccgtaggtcccggaccttggg 4020
TAGCGCTGGGGCAGGCGGGGCAACCTGCGGGGAGTCCCTGGCATCCAGGGCCTGGAACCC

4021 tctttctaccagaggtgctcggaggctcgcggtcagtccgacccggagtctctcgactca 4080
AGAAAGATGGTCTCCACGAGCCTCCGAGCGCCAGTCAGGCTGGGCCTCAGAGAGCTGAGT

4081 tccttcctcccggcgtcgacgaggaacagcggactcctcatctccttcacgaaccagagc 4140
AGGAAGGAGGGCCGCAGCTGCTCCTTGTCGCCTGAGGAGTAGAGGAAGTGCTTGGTCTCG

ORF_1 rf(1)(4144,4869)>>> 
|
4141 cgcatgtggccccctgttccgcacagggtccctgcaccaccggcgctacacctacccccc 4200
GCGTACACCGGGGGACAAGGCGTGTCCCAGGGACGTGGTGGCCGCGATGTGGATGGGGGG

4201 gggcgcaccacgaccgccgggtgcctacccaccctcaccgcgcacggtctctcgcgtggg 4260
CCCGCGTGGTGCTGGCGGCCCACGGATGGGTGGGAGTGGCGCGTGCCAGAGAGCGCACCC

4261 aggtttctccaccgaagaagccgcccagaccgtccactgtggtgtgtctttggtgccagt 4320
TCCAAAGAGGTGGCTTCTTCGGCGGGTCTGGCAGGTGACACCACACAGAAACCACGGTCA

4321 gagccaggtgcgcaggacgggcccacccgggtcctggggacgggttgcccgcaggcgagg 4380
CTCGGTCCACGCGTCCTGCCCGGGTGGGCCCAGGACCCCTGCCCAACGGGCGTCCGCTCC

4381 ccgagtccccgtcgcggtgcggacccggagaacccgttgccgtctgaagccgaccgtgac 4440
GGCTCAGGGGCAGCGCCACGCCTGGGCCTCTTGGGCAACGGCAGACTTCGGCTGGCACTG

4441 gggggcgcggaggagcgtgggccccgaccgtccgggtccccctggggccggagggactgc 4500
CCCCCGCGCCTCCTCGCACCCGGGGCTGGCAGGCCCAGGGGGACCCCGGCCTCCCTGACG

4501 gataccaaggtccgggcaagcgtagggtctgcggaagccccaggtgatcgcacaccgccc 4560
CTATGGTTCCAGGCCCGTTCGCATCCCAGACGCCTTCGGGGTCCACTAGCGTGTGGCGGG

4561 ccggcccggactcaccgtcgcggctcgaccatgtcgccgccgggcgtgtggaccatccgc 4620
GGCCGGGCCTGAGTGGCAGCGCCGAGCTGGTACAGCGGCGGCCCGCACACCTGGTAGGCG

4621 gtcgaccctcggtggtcgtgtttctcgcgcgtcgcacggtcgtccacttggtcgtgcagc 4680
CAGCTGGGAGCCACCAGCACAAAGAGCGCGCAGCGTGCCAGCAGGTGAACCAGCACGTCG

4681 agcgggtgcgccgcgtcgtcgtcgggggtgcggggcgagggggcgtcacgcagccagtgg 4740
TCGCCCACGCGGCGCAGCAGCAGCCCCCACGCCCCGCTCCCCCGCAGTGCGTCGGTCACC

4741 cacaacccgtccatcgacgcgtgcgaccaccacttccggagcccccccgggggcgcccgg 4800
GTGTTGGGCAGGTAGCTGCGCACGCTGGTGGTGAAGGCCTCGGGGGGGCCCCCGCGGGCC

4801 ggcaggtcgtcgcgcttcggcttccggtcgtgcaagaagcgcggcgcgagcgtgtcggag 4860
CCGTCCAGCAGCGCGAAGCCGAAGGCCAGCACGTTCTTCGCGCCGCGCTCGCACAGCCTC

4861 acgtcgtgagcccggtggtcgaggaagtccgtcctgtggaccgccttcctcccccgccgc 4920
TGCAGCACTCGGGCCACCAGCTCCTTCAGGCAGGACACCTGGCGGAAGGAGGGGGCGGCG

4921 cccccgccggcacgcagggtcccgtgcgtgtggtccgtgacccggtggtcgcgcgccttt 4980
GGGGGCGGCCGTGCGTCCCAGGGCACGCACACCAGGCACTGGGCCACCAGCGCGCGGAAA

4981 cggcggcccaggggcgcgacgtggtcggcggtcgggaccccggggtccgcggcgtgcttg 5040
GCCGCCGGGTCCCCGCGCTGCACCAGCCGCCAGCCCTGGGGCCCCAGGCGCCGCACGAAC

5041 caccggtcgccgtcgtggagcgccatcaccgacgcgtcgtccctcgcgtgccgagccgtc 5100
GTGGCCAGCGGCAGCACCTCGCGGTAGTGGCTGCGCAGCAGGGAGCGCACGGCTCGGCAG

5101 gcccctcgcgcgccgtagcgcccccaccggccccggtcccgaagggtgcacgcgtcgtcc 5160
CGGGGAGCGCGCGGCATCGCGGGGGTGGCCGGGGCCAGGGCTTCCCACGTGCGCAGCAGG

EcoRV NotI XbaI 
| | |
5161 tgcgtcgcgacggacccctgcagatatccagcacagtggcggccgctcgagtctagaggg 5220
ACGCAGCGCTGCCTGGGGACGTCTATAGGTCGTGTCACCGCCGGCGAGCTCAGATCTCCC

5221 cccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttt 5280
GGGCAAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAA

5281 gcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaat 5340
CGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTA

5341 aaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtgggg 5400
TTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCC

5401 tggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcgg 5460
ACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCC

ClaI 
|
5461 tgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcttatgggtattat 5520
ACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGAATACCCATAATA

5521 gggtctgcattaatgaatcggccaacggatcccggtgtgaaataccgcacagatgcgtaa 5580
CCCAGACGTAATTACTTAGCCGGTTGCCTAGGGCCACACTTTATGGCGTGTCTACGCATT

5581 ggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcgg 5640
CCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCC

Kan/Neo(5661,6441)>>> 
|
5641 tcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacag 5700
AGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTC

5701 aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc 5760
TTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGG

pBR322 origin(5771,6390)<<< 
|
5761 gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcaca 5820
CATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGT

5821 aaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgt 5880
TTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCA

5881 ttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacc 5940
AAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGG

5941 tgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc 6000
ACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAG

6001 tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagc 6060
AGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCG

6061 ccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact 6120
GGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGA

6121 tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtg 6180
ATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCAC

6181 ctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta 6240
GATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCAT

6241 tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca 6300
AGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGT

6301 aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa 6360
TTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTT

6361 aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg 6420
TTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGC

6421 aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc 6480
TTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGG

6481 ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctg 6540
AAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGAC

6541 acagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcat 6600
TGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTA

6601 ccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctg 6660
GGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGAC

6661 gccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa 6720
CGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTT

6721 taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcca 6780
ATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGT

6781 tccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgc 6840
AGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACG

6841 gcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgca 6900
CGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGT

6901 gaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgc 6960
CTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCG

6961 aagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggc 7020
TTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCG

NEOKAN prom(7043,7092)>>> 
|
7021 ggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgg 7080
CCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACC

7081 gaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcagggg 7140
CTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCC

NTP_II marker(7184,7972)>>> 
|
ORF_2 rf(2)(7181,7975)>>> 
| |
7141 atcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggatt 7200
TAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAA

7201 gcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaaca 7260
CGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGT

7261 gacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttct 7320
CTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGA

7321 ttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggct 7380
AAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGA

7381 atcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagc 7440
TAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCG

7441 gggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 7500
CCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGA

7501 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttga 7560
ACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACT

7561 tccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcg 7620
AGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGC

7621 gatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcc 7680
CTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGG

7681 agccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgac 7740
TCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTG

7741 ccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcat 7800
GGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTA

7801 cgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtga 7860
GCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACT

7861 tattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgc 7920
ATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCG

7921 cgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaatttt 7980
GCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAA

7981 gttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcc 8040
CAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGG

f1 origin(8080,8385)<<< 
|
8041 cttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaaga 8100
GAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCT

8101 gtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcg 8160
CAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGC

8161 atggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaag 8220
TACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTC

8221 ctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcga 8280
GAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCT

8281 acgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtg 8340
TGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCAC

8341 tagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgc 8400
ATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCG

8401 gtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 8459
CAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA

                            Misc. Feature (4 total)
CMV promoter
Start: 626 End: 1366
Original Location Description:
626..1366
hTERT
Start: 1457 End: 5170
ORI
Start: 5662 End: 6441
Original Location Description:
1942..2721
Kan
Start: 7187 End: 7978
Original Location Description:
3467..4258