pAdeno-hTERT
| Vector Name | pAdeno-hTERT |
| VectorType | Adenoviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | Sequencing primer: (1311-13300) 5`---GATAGAGATCGTCGACGAGC ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 360
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 420
421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg 480
481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 540
541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 600
601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 660
661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 720
721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 780
781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 840
841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 900
901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 960
961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 1020
1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 1080
1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 1140
1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa 1200
1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 1260
1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc 1320
1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 1380
1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc 1440
1441 agtgtggtgg aattcaggca gcgctgcgtc ctgctgcgca cgtgggaagc cctggccccg 1500
1501 gccacccccg cgatgccgcg cgctccccgc tgccgagccg tgcgctccct gctgcgcagc 1560
1561 cactaccgcg aggtgctgcc gctggccacg ttcgtgcggc gcctggggcc ccagggctgg 1620
1621 cggctggtgc agcgcgggga cccggcggct ttccgcgcgc tggtggccca gtgcctggtg 1680
1681 tgcgtgccct gggacgcacg gccgcccccc gccgccccct ccttccgcca ggtgtcctgc 1740
1741 ctgaaggagc tggtggcccg agtgctgcag aggctgtgcg agcgcggcgc gaagaacgtg 1800
1801 ctggccttcg gcttcgcgct gctggacggg gcccgcgggg gcccccccga ggccttcacc 1860
1861 accagcgtgc gcagctacct gcccaacacg gtgaccgacg cactgcgggg gagcggggcg 1920
1921 tgggggctgc tgctgcgccg cgtgggcgac gacgtgctgg ttcacctgct ggcacgctgc 1980
1981 gcgctctttg tgctggtggc tcccagctgc gcctaccagg tgtgcgggcc gccgctgtac 2040
2041 cagctcggcg ctgccactca ggcccggccc ccgccacacg ctagtggacc ccgaaggcgt 2100
2101 ctgggatgcg aacgggcctg gaaccatagc gtcagggagg ccggggtccc cctgggcctg 2160
2161 ccagccccgg gtgcgaggag gcgcgggggc agtgccagcc gaagtctgcc gttgcccaag 2220
2221 aggcccaggc gtggcgctgc ccctgagccg gagcggacgc ccgttgggca ggggtcctgg 2280
2281 gcccacccgg gcaggacgcg tggaccgagt gaccgtggtt tctgtgtggt gtcacctgcc 2340
2341 agacccgccg aagaagccac ctctttggag ggtgcgctct ctggcacgcg ccactcccac 2400
2401 ccatccgtgg gccgccagca ccacgcgggc cccccatcca catcgcggcc accacgtccc 2460
2461 tgggacacgc cttgtccccc ggtgtacgcc gagaccaagc acttcctcta ctcctcaggc 2520
2521 gacaaggagc agctgcggcc ctccttccta ctcagctctc tgaggcccag cctgactggc 2580
2581 gctcggaggc tcgtggagac catctttctg ggttccaggc cctggatgcc agggactccc 2640
2641 cgcaggttgc cccgcctgcc ccagcgctac tggcaaatgc ggcccctgtt tctggagctg 2700
2701 cttgggaacc acgcgcagtg cccctacggg gtgctcctca agacgcactg cccgctgcga 2760
2761 gctgcggtca ccccagcagc cggtgtctgt gcccgggaga agccccaggg ctctgtggcg 2820
2821 gcccccgagg aggaggacac agacccccgt cgcctggtgc agctgctccg ccagcacagc 2880
2881 agcccctggc aggtgtacgg cttcgtgcgg gcctgcctgc gccggctggt gcccccaggc 2940
2941 ctctggggct ccaggcacaa cgaacgccgc ttcctcagga acaccaagaa gttcatctcc 3000
3001 ctggggaagc atgccaagct ctcgctgcag gagctgacgt ggaagatgag cgtgcgggac 3060
3061 tgcgcttggc tgcgcaggag cccaggggtt ggctgtgttc cggccgcaga gcaccgtctg 3120
3121 cgtgaggaga tcctggccaa gttcctgcac tggctgatga gtgtgtacgt cgtcgagctg 3180
3181 ctcaggtctt tcttttatgt cacggagacc acgtttcaaa agaacaggct ctttttctac 3240
3241 cggaagagtg tctggagcaa gttgcaaagc attggaatca gacagcactt gaagagggtg 3300
3301 cagctgcggg agctgtcgga agcagaggtc aggcagcatc gggaagccag gcccgccctg 3360
3361 ctgacgtcca gactccgctt catccccaag cctgacgggc tgcggccgat tgtgaacatg 3420
3421 gactacgtcg tgggagccag aacgttccgc agagaaaaga gggccgagcg tctcacctcg 3480
3481 agggtgaagg cactgttcag cgtgctcaac tacgagcggg cgcggcgccc cggcctcctg 3540
3541 ggcgcctctg tgctgggcct ggacgatatc cacagggcct ggcgcacctt cgtgctgcgt 3600
3601 gtgcgggccc aggacccgcc gcctgagctg tactttgtca aggtggatgt gacgggcgcg 3660
3661 tacgacacca tcccccagga caggctcacg gaggtcatcg ccagcatcat caaaccccag 3720
3721 aacacgtact gcgtgcgtcg gtatgccgtg gtccagaagg ccgcccatgg gcacgtccgc 3780
3781 aaggccttca agagccacgt ctctaccttg acagacctcc agccgtacat gcgacagttc 3840
3841 gtggctcacc tgcaggagac cagcccgctg agggatgccg tcgtcatcga gcagagctcc 3900
3901 tccctgaatg aggccagcag tggcctcttc gacgtcttcc tacgcttcat gtgccaccac 3960
3961 gccgtgcgca tcaggggcaa gtcctacgtc cagtgccagg ggatcccgca gggctccatc 4020
4021 ctctccacgc tgctctgcag cctgtgctac ggcgacatgg agaacaagct gtttgcgggg 4080
4081 attcggcggg acgggctgct cctgcgtttg gtggatgatt tcttgttggt gacacctcac 4140
4141 ctcacccacg cgaaaacctt cctcaggacc ctggtccgag gtgtccctga gtatggctgc 4200
4201 gtggtgaact tgcggaagac agtggtgaac ttccctgtag aagacgaggc cctgggtggc 4260
4261 acggcttttg ttcagatgcc ggcccacggc ctattcccct ggtgcggcct gctgctggat 4320
4321 acccggaccc tggaggtgca gagcgactac tccagctatg cccggacctc catcagagcc 4380
4381 agtctcacct tcaaccgcgg cttcaaggct gggaggaaca tgcgtcgcaa actctttggg 4440
4441 gtcttgcggc tgaagtgtca cagcctgttt ctggatttgc aggtgaacag cctccagacg 4500
4501 gtgtgcacca acatctacaa gatcctcctg ctgcaggcgt acaggtttca cgcatgtgtg 4560
4561 ctgcagctcc catttcatca gcaagtttgg aagaacccca catttttcct gcgcgtcatc 4620
4621 tctgacacgg cctccctctg ctactccatc ctgaaagcca agaacgcagg gatgtcgctg 4680
4681 ggggccaagg gcgccgccgg ccctctgccc tccgaggccg tgcagtggct gtgccaccaa 4740
4741 gcattcctgc tcaagctgac tcgacaccgt gtcacctacg tgccactcct ggggtcactc 4800
4801 aggacagccc agacgcagct gagtcggaag ctcccgggga cgacgctgac tgccctggag 4860
4861 gccgcagcca acccggcact gccctcagac ttcaagacca tcctggactg atggccaccc 4920
4921 gcccacagcc aggccgagag cagacaccag cagccctgtc acgccgggct ctacgtccca 4980
4981 gggagggagg ggcggcccac acccaggccc gcaccgctgg gagtctgagg cctgagtgag 5040
5041 tgtttggccg aggcctgcat gtccggctga aggctgagtg tccggctgag gcctgagcga 5100
5101 gtgtccagcc aagggctgag tgtccagcac acctgccgtc ttcacttccc cacaggctgg 5160
5161 cgctcggctc caccctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc 5220
5221 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 5280
5281 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 5340
5341 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 5400
5401 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 5460
5461 gctctatggc ttctgaggcg gaaagaacca gcagatcgat ctgcttatgg gtattatggg 5520
5521 tctgcattaa tgaatcggcc aacggatccc ggtgtgaaat accgcacaga tgcgtaagga 5580
5581 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5640
5641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5700
5701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5760
5761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5820
5821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5880
5881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5940
5941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 6000
6001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6060
6061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6120
6121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6180
6181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 6240
6241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6300
6301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 6360
6361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 6420
6421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 6480
6481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 6540
6541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 6600
6601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 6660
6661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 6720
6721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6780
6781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6840
6841 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 6900
6901 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 6960
6961 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 7020
7021 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 7080
7081 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc 7140
7141 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 7200
7201 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 7260
7261 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 7320
7321 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 7380
7381 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 7440
7441 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 7500
7501 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 7560
7561 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 7620
7621 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 7680
7681 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 7740
7741 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 7800
7801 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 7860
7861 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 7920
7921 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt 7980
7981 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt 8040
8041 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc 8100
8101 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 8160
8161 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc 8220
8221 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 8280
8281 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 8340
8341 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc 8400
8401 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt 8456
LOCUS dna 8456 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 665..670
/dbxref="REBASE:NruI"
misc_binding 942..947
/dbxref="REBASE:NdeI"
Regulatory_Seq 1279..1318
/gene="tetO reg"
ORF 1306..2127
/sequence="ORF_4 rf(6)"
misc_binding 1409..1414
/dbxref="REBASE:HindIII"
misc_binding 1415..1420
/dbxref="REBASE:KpnI"
misc_binding 1450..1455
/dbxref="REBASE:EcoRI"
promoter 1456..5170
/gene="hTERT"
ORF 1513..4911
/sequence="ORF_1 rf(1)"
ORF 2548..3768
/sequence="ORF_3 rf(6)"
misc_binding 5196..5203
/dbxref="REBASE:NotI"
misc_binding 5209..5214
/dbxref="REBASE:XbaI"
misc_binding 5495..5500
/dbxref="REBASE:ClaI"
selectable_marker661..6441
/gene="Kan/Neo"
Rep_Origin 5768..6387
/gene="pBR322 origin"
Promoter 7040..7089
/gene="NEOKAN prom"
ORF 7178..7972
/sequence="ORF_2 rf(2)"
Marker 7181..7969
/gene="NTP_II marker"
Rep_Origin 8077..8382
/gene="f1 origin"
BASE COUNT 1748 a 2431 c 2491 g 1786 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg
481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa
1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc
1441 agtgtggtgg aattcaggca gcgctgcgtc ctgctgcgca cgtgggaagc cctggccccg
1501 gccacccccg cgatgccgcg cgctccccgc tgccgagccg tgcgctccct gctgcgcagc
1561 cactaccgcg aggtgctgcc gctggccacg ttcgtgcggc gcctggggcc ccagggctgg
1621 cggctggtgc agcgcgggga cccggcggct ttccgcgcgc tggtggccca gtgcctggtg
1681 tgcgtgccct gggacgcacg gccgcccccc gccgccccct ccttccgcca ggtgtcctgc
1741 ctgaaggagc tggtggcccg agtgctgcag aggctgtgcg agcgcggcgc gaagaacgtg
1801 ctggccttcg gcttcgcgct gctggacggg gcccgcgggg gcccccccga ggccttcacc
1861 accagcgtgc gcagctacct gcccaacacg gtgaccgacg cactgcgggg gagcggggcg
1921 tgggggctgc tgctgcgccg cgtgggcgac gacgtgctgg ttcacctgct ggcacgctgc
1981 gcgctctttg tgctggtggc tcccagctgc gcctaccagg tgtgcgggcc gccgctgtac
2041 cagctcggcg ctgccactca ggcccggccc ccgccacacg ctagtggacc ccgaaggcgt
2101 ctgggatgcg aacgggcctg gaaccatagc gtcagggagg ccggggtccc cctgggcctg
2161 ccagccccgg gtgcgaggag gcgcgggggc agtgccagcc gaagtctgcc gttgcccaag
2221 aggcccaggc gtggcgctgc ccctgagccg gagcggacgc ccgttgggca ggggtcctgg
2281 gcccacccgg gcaggacgcg tggaccgagt gaccgtggtt tctgtgtggt gtcacctgcc
2341 agacccgccg aagaagccac ctctttggag ggtgcgctct ctggcacgcg ccactcccac
2401 ccatccgtgg gccgccagca ccacgcgggc cccccatcca catcgcggcc accacgtccc
2461 tgggacacgc cttgtccccc ggtgtacgcc gagaccaagc acttcctcta ctcctcaggc
2521 gacaaggagc agctgcggcc ctccttccta ctcagctctc tgaggcccag cctgactggc
2581 gctcggaggc tcgtggagac catctttctg ggttccaggc cctggatgcc agggactccc
2641 cgcaggttgc cccgcctgcc ccagcgctac tggcaaatgc ggcccctgtt tctggagctg
2701 cttgggaacc acgcgcagtg cccctacggg gtgctcctca agacgcactg cccgctgcga
2761 gctgcggtca ccccagcagc cggtgtctgt gcccgggaga agccccaggg ctctgtggcg
2821 gcccccgagg aggaggacac agacccccgt cgcctggtgc agctgctccg ccagcacagc
2881 agcccctggc aggtgtacgg cttcgtgcgg gcctgcctgc gccggctggt gcccccaggc
2941 ctctggggct ccaggcacaa cgaacgccgc ttcctcagga acaccaagaa gttcatctcc
3001 ctggggaagc atgccaagct ctcgctgcag gagctgacgt ggaagatgag cgtgcgggac
3061 tgcgcttggc tgcgcaggag cccaggggtt ggctgtgttc cggccgcaga gcaccgtctg
3121 cgtgaggaga tcctggccaa gttcctgcac tggctgatga gtgtgtacgt cgtcgagctg
3181 ctcaggtctt tcttttatgt cacggagacc acgtttcaaa agaacaggct ctttttctac
3241 cggaagagtg tctggagcaa gttgcaaagc attggaatca gacagcactt gaagagggtg
3301 cagctgcggg agctgtcgga agcagaggtc aggcagcatc gggaagccag gcccgccctg
3361 ctgacgtcca gactccgctt catccccaag cctgacgggc tgcggccgat tgtgaacatg
3421 gactacgtcg tgggagccag aacgttccgc agagaaaaga gggccgagcg tctcacctcg
3481 agggtgaagg cactgttcag cgtgctcaac tacgagcggg cgcggcgccc cggcctcctg
3541 ggcgcctctg tgctgggcct ggacgatatc cacagggcct ggcgcacctt cgtgctgcgt
3601 gtgcgggccc aggacccgcc gcctgagctg tactttgtca aggtggatgt gacgggcgcg
3661 tacgacacca tcccccagga caggctcacg gaggtcatcg ccagcatcat caaaccccag
3721 aacacgtact gcgtgcgtcg gtatgccgtg gtccagaagg ccgcccatgg gcacgtccgc
3781 aaggccttca agagccacgt ctctaccttg acagacctcc agccgtacat gcgacagttc
3841 gtggctcacc tgcaggagac cagcccgctg agggatgccg tcgtcatcga gcagagctcc
3901 tccctgaatg aggccagcag tggcctcttc gacgtcttcc tacgcttcat gtgccaccac
3961 gccgtgcgca tcaggggcaa gtcctacgtc cagtgccagg ggatcccgca gggctccatc
4021 ctctccacgc tgctctgcag cctgtgctac ggcgacatgg agaacaagct gtttgcgggg
4081 attcggcggg acgggctgct cctgcgtttg gtggatgatt tcttgttggt gacacctcac
4141 ctcacccacg cgaaaacctt cctcaggacc ctggtccgag gtgtccctga gtatggctgc
4201 gtggtgaact tgcggaagac agtggtgaac ttccctgtag aagacgaggc cctgggtggc
4261 acggcttttg ttcagatgcc ggcccacggc ctattcccct ggtgcggcct gctgctggat
4321 acccggaccc tggaggtgca gagcgactac tccagctatg cccggacctc catcagagcc
4381 agtctcacct tcaaccgcgg cttcaaggct gggaggaaca tgcgtcgcaa actctttggg
4441 gtcttgcggc tgaagtgtca cagcctgttt ctggatttgc aggtgaacag cctccagacg
4501 gtgtgcacca acatctacaa gatcctcctg ctgcaggcgt acaggtttca cgcatgtgtg
4561 ctgcagctcc catttcatca gcaagtttgg aagaacccca catttttcct gcgcgtcatc
4621 tctgacacgg cctccctctg ctactccatc ctgaaagcca agaacgcagg gatgtcgctg
4681 ggggccaagg gcgccgccgg ccctctgccc tccgaggccg tgcagtggct gtgccaccaa
4741 gcattcctgc tcaagctgac tcgacaccgt gtcacctacg tgccactcct ggggtcactc
4801 aggacagccc agacgcagct gagtcggaag ctcccgggga cgacgctgac tgccctggag
4861 gccgcagcca acccggcact gccctcagac ttcaagacca tcctggactg atggccaccc
4921 gcccacagcc aggccgagag cagacaccag cagccctgtc acgccgggct ctacgtccca
4981 gggagggagg ggcggcccac acccaggccc gcaccgctgg gagtctgagg cctgagtgag
5041 tgtttggccg aggcctgcat gtccggctga aggctgagtg tccggctgag gcctgagcga
5101 gtgtccagcc aagggctgag tgtccagcac acctgccgtc ttcacttccc cacaggctgg
5161 cgctcggctc caccctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
5221 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
5281 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
5341 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
5401 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
5461 gctctatggc ttctgaggcg gaaagaacca gcagatcgat ctgcttatgg gtattatggg
5521 tctgcattaa tgaatcggcc aacggatccc ggtgtgaaat accgcacaga tgcgtaagga
5581 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
5641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
5701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
5821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
5881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
5941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
6001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
6061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
6121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
6181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
6241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
6301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
6361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
6421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
6481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6841 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa
6901 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
6961 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
7021 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
7081 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc
7141 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
7201 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
7261 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
7321 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
7381 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
7441 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
7501 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
7561 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
7621 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
7681 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
7741 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
7801 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
7861 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
7921 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt
7981 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt
8041 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc
8101 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
8161 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc
8221 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
8281 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
8341 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc
8401 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
421 gttccgcgcacatttccccgaaaagtgccacctgacgtaagctcagatctggatctcccg 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTCGAGTCTAGACCTAGAGGGC
481 atcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatc 540
TAGGGGATACCAGCTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATAG
541 tgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaa 600
ACGAGGGACGAACACACAACCTCCAGCGACTCATCACGCGCTCGTTTTAAATTCGATGTT
601 caaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgc 660
GTTCCGTTCCGAACTGGCTGTTAACGTACTTCTTAGACGAATCCCAATCCGCAAAACGCG
NruI
|
661 tgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaat 720
ACGAAGCGCTACATGCCCGGTCTATATGCGCAACTGTAACTAATAACTGATCAATAATTA
721 agtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataac 780
TCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTG
781 ttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataa 840
AATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATT
841 tgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagt 900
ACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCA
NdeI
|
901 atttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgcccc 960
TAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGG
961 ctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttat 1020
GATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATA
1021 gggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgc 1080
CCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACG
1081 ggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc 1140
CCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAG
1141 tccaccccattgacgtcaatgggagtttgttttggaaccaaaatcaacgggactttccaa 1200
AGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCTTGGTTTTAGTTGCCCTGAAAGGTT
1201 aatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggagg 1260
TTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCC
tetO reg(1279,1318)>>> ORF_4 rf(6)(1306,2127)<<<
| |
1261 tctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatc 1320
AGATATATTCGTCTCGAGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTAG
1321 gtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttg 1380
CAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAAC
HindIII KpnI
| |
1381 acctccatagaagctagcgtttaaacttaagcttggtaccgagctcggatccactagtcc 1440
TGGAGGTATCTTCGATCGCAAATTTGAATTCGAACCATGGCTCGAGCCTAGGTGATCAGG
hTERT(1456,5170)>>>
|
EcoRI
| |
1441 agtgtggtggaattcaggcagcgctgcgtcctgctgcgcacgtgggaagccctggccccg 1500
TCACACCACCTTAAGTCCGTCGCGACGCAGGACGACGCGTGCACCCTTCGGGACCGGGGC
ORF_1 rf(1)(1513,4911)>>>
|
1501 gccacccccgcgatgccgcgcgctccccgctgccgagccgtgcgctccctgctgcgcagc 1560
CGGTGGGGGCGCTACGGCGCGCGAGGGGCGACGGCTCGGCACGCGAGGGACGACGCGTCG
1561 cactaccgcgaggtgctgccgctggccacgttcgtgcggcgcctggggccccagggctgg 1620
GTGATGGCGCTCCACGACGGCGACCGGTGCAAGCACGCCGCGGACCCCGGGGTCCCGACC
1621 cggctggtgcagcgcggggacccggcggctttccgcgcgctggtggcccagtgcctggtg 1680
GCCGACCACGTCGCGCCCCTGGGCCGCCGAAAGGCGCGCGACCACCGGGTCACGGACCAC
1681 tgcgtgccctgggacgcacggccgccccccgccgccccctccttccgccaggtgtcctgc 1740
ACGCACGGGACCCTGCGTGCCGGCGGGGGGCGGCGGGGGAGGAAGGCGGTCCACAGGACG
1741 ctgaaggagctggtggcccgagtgctgcagaggctgtgcgagcgcggcgcgaagaacgtg 1800
GACTTCCTCGACCACCGGGCTCACGACGTCTCCGACACGCTCGCGCCGCGCTTCTTGCAC
1801 ctggccttcggcttcgcgctgctggacggggcccgcgggggcccccccgaggccttcacc 1860
GACCGGAAGCCGAAGCGCGACGACCTGCCCCGGGCGCCCCCGGGGGGGCTCCGGAAGTGG
1861 accagcgtgcgcagctacctgcccaacacggtgaccgacgcactgcgggggagcggggcg 1920
TGGTCGCACGCGTCGATGGACGGGTTGTGCCACTGGCTGCGTGACGCCCCCTCGCCCCGC
1921 tgggggctgctgctgcgccgcgtgggcgacgacgtgctggttcacctgctggcacgctgc 1980
ACCCCCGACGACGACGCGGCGCACCCGCTGCTGCACGACCAAGTGGACGACCGTGCGACG
1981 gcgctctttgtgctggtggctcccagctgcgcctaccaggtgtgcgggccgccgctgtac 2040
CGCGAGAAACACGACCACCGAGGGTCGACGCGGATGGTCCACACGCCCGGCGGCGACATG
2041 cagctcggcgctgccactcaggcccggcccccgccacacgctagtggaccccgaaggcgt 2100
GTCGAGCCGCGACGGTGAGTCCGGGCCGGGGGCGGTGTGCGATCACCTGGGGCTTCCGCA
2101 ctgggatgcgaacgggcctggaaccatagcgtcagggaggccggggtccccctgggcctg 2160
GACCCTACGCTTGCCCGGACCTTGGTATCGCAGTCCCTCCGGCCCCAGGGGGACCCGGAC
2161 ccagccccgggtgcgaggaggcgcgggggcagtgccagccgaagtctgccgttgcccaag 2220
GGTCGGGGCCCACGCTCCTCCGCGCCCCCGTCACGGTCGGCTTCAGACGGCAACGGGTTC
2221 aggcccaggcgtggcgctgcccctgagccggagcggacgcccgttgggcaggggtcctgg 2280
TCCGGGTCCGCACCGCGACGGGGACTCGGCCTCGCCTGCGGGCAACCCGTCCCCAGGACC
2281 gcccacccgggcaggacgcgtggaccgagtgaccgtggtttctgtgtggtgtcacctgcc 2340
CGGGTGGGCCCGTCCTGCGCACCTGGCTCACTGGCACCAAAGACACACCACAGTGGACGG
2341 agacccgccgaagaagccacctctttggagggtgcgctctctggcacgcgccactcccac 2400
TCTGGGCGGCTTCTTCGGTGGAGAAACCTCCCACGCGAGAGACCGTGCGCGGTGAGGGTG
2401 ccatccgtgggccgccagcaccacgcgggccccccatccacatcgcggccaccacgtccc 2460
GGTAGGCACCCGGCGGTCGTGGTGCGCCCGGGGGGTAGGTGTAGCGCCGGTGGTGCAGGG
2461 tgggacacgccttgtcccccggtgtacgccgagaccaagcacttcctctactcctcaggc 2520
ACCCTGTGCGGAACAGGGGGCCACATGCGGCTCTGGTTCGTGAAGGAGATGAGGAGTCCG
ORF_3 rf(6)(2548,3768)<<<
|
2521 gacaaggagcagctgcggccctccttcctactcagctctctgaggcccagcctgactggc 2580
CTGTTCCTCGTCGACGCCGGGAGGAAGGATGAGTCGAGAGACTCCGGGTCGGACTGACCG
2581 gctcggaggctcgtggagaccatctttctgggttccaggccctggatgccagggactccc 2640
CGAGCCTCCGAGCACCTCTGGTAGAAAGACCCAAGGTCCGGGACCTACGGTCCCTGAGGG
2641 cgcaggttgccccgcctgccccagcgctactggcaaatgcggcccctgtttctggagctg 2700
GCGTCCAACGGGGCGGACGGGGTCGCGATGACCGTTTACGCCGGGGACAAAGACCTCGAC
2701 cttgggaaccacgcgcagtgcccctacggggtgctcctcaagacgcactgcccgctgcga 2760
GAACCCTTGGTGCGCGTCACGGGGATGCCCCACGAGGAGTTCTGCGTGACGGGCGACGCT
2761 gctgcggtcaccccagcagccggtgtctgtgcccgggagaagccccagggctctgtggcg 2820
CGACGCCAGTGGGGTCGTCGGCCACAGACACGGGCCCTCTTCGGGGTCCCGAGACACCGC
2821 gcccccgaggaggaggacacagacccccgtcgcctggtgcagctgctccgccagcacagc 2880
CGGGGGCTCCTCCTCCTGTGTCTGGGGGCAGCGGACCACGTCGACGAGGCGGTCGTGTCG
2881 agcccctggcaggtgtacggcttcgtgcgggcctgcctgcgccggctggtgcccccaggc 2940
TCGGGGACCGTCCACATGCCGAAGCACGCCCGGACGGACGCGGCCGACCACGGGGGTCCG
2941 ctctggggctccaggcacaacgaacgccgcttcctcaggaacaccaagaagttcatctcc 3000
GAGACCCCGAGGTCCGTGTTGCTTGCGGCGAAGGAGTCCTTGTGGTTCTTCAAGTAGAGG
3001 ctggggaagcatgccaagctctcgctgcaggagctgacgtggaagatgagcgtgcgggac 3060
GACCCCTTCGTACGGTTCGAGAGCGACGTCCTCGACTGCACCTTCTACTCGCACGCCCTG
3061 tgcgcttggctgcgcaggagcccaggggttggctgtgttccggccgcagagcaccgtctg 3120
ACGCGAACCGACGCGTCCTCGGGTCCCCAACCGACACAAGGCCGGCGTCTCGTGGCAGAC
3121 cgtgaggagatcctggccaagttcctgcactggctgatgagtgtgtacgtcgtcgagctg 3180
GCACTCCTCTAGGACCGGTTCAAGGACGTGACCGACTACTCACACATGCAGCAGCTCGAC
3181 ctcaggtctttcttttatgtcacggagaccacgtttcaaaagaacaggctctttttctac 3240
GAGTCCAGAAAGAAAATACAGTGCCTCTGGTGCAAAGTTTTCTTGTCCGAGAAAAAGATG
3241 cggaagagtgtctggagcaagttgcaaagcattggaatcagacagcacttgaagagggtg 3300
GCCTTCTCACAGACCTCGTTCAACGTTTCGTAACCTTAGTCTGTCGTGAACTTCTCCCAC
3301 cagctgcgggagctgtcggaagcagaggtcaggcagcatcgggaagccaggcccgccctg 3360
GTCGACGCCCTCGACAGCCTTCGTCTCCAGTCCGTCGTAGCCCTTCGGTCCGGGCGGGAC
3361 ctgacgtccagactccgcttcatccccaagcctgacgggctgcggccgattgtgaacatg 3420
GACTGCAGGTCTGAGGCGAAGTAGGGGTTCGGACTGCCCGACGCCGGCTAACACTTGTAC
3421 gactacgtcgtgggagccagaacgttccgcagagaaaagagggccgagcgtctcacctcg 3480
CTGATGCAGCACCCTCGGTCTTGCAAGGCGTCTCTTTTCTCCCGGCTCGCAGAGTGGAGC
3481 agggtgaaggcactgttcagcgtgctcaactacgagcgggcgcggcgccccggcctcctg 3540
TCCCACTTCCGTGACAAGTCGCACGAGTTGATGCTCGCCCGCGCCGCGGGGCCGGAGGAC
3541 ggcgcctctgtgctgggcctggacgatatccacagggcctggcgcaccttcgtgctgcgt 3600
CCGCGGAGACACGACCCGGACCTGCTATAGGTGTCCCGGACCGCGTGGAAGCACGACGCA
3601 gtgcgggcccaggacccgccgcctgagctgtactttgtcaaggtggatgtgacgggcgcg 3660
CACGCCCGGGTCCTGGGCGGCGGACTCGACATGAAACAGTTCCACCTACACTGCCCGCGC
3661 tacgacaccatcccccaggacaggctcacggaggtcatcgccagcatcatcaaaccccag 3720
ATGCTGTGGTAGGGGGTCCTGTCCGAGTGCCTCCAGTAGCGGTCGTAGTAGTTTGGGGTC
3721 aacacgtactgcgtgcgtcggtatgccgtggtccagaaggccgcccatgggcacgtccgc 3780
TTGTGCATGACGCACGCAGCCATACGGCACCAGGTCTTCCGGCGGGTACCCGTGCAGGCG
3781 aaggccttcaagagccacgtctctaccttgacagacctccagccgtacatgcgacagttc 3840
TTCCGGAAGTTCTCGGTGCAGAGATGGAACTGTCTGGAGGTCGGCATGTACGCTGTCAAG
3841 gtggctcacctgcaggagaccagcccgctgagggatgccgtcgtcatcgagcagagctcc 3900
CACCGAGTGGACGTCCTCTGGTCGGGCGACTCCCTACGGCAGCAGTAGCTCGTCTCGAGG
3901 tccctgaatgaggccagcagtggcctcttcgacgtcttcctacgcttcatgtgccaccac 3960
AGGGACTTACTCCGGTCGTCACCGGAGAAGCTGCAGAAGGATGCGAAGTACACGGTGGTG
3961 gccgtgcgcatcaggggcaagtcctacgtccagtgccaggggatcccgcagggctccatc 4020
CGGCACGCGTAGTCCCCGTTCAGGATGCAGGTCACGGTCCCCTAGGGCGTCCCGAGGTAG
4021 ctctccacgctgctctgcagcctgtgctacggcgacatggagaacaagctgtttgcgggg 4080
GAGAGGTGCGACGAGACGTCGGACACGATGCCGCTGTACCTCTTGTTCGACAAACGCCCC
4081 attcggcgggacgggctgctcctgcgtttggtggatgatttcttgttggtgacacctcac 4140
TAAGCCGCCCTGCCCGACGAGGACGCAAACCACCTACTAAAGAACAACCACTGTGGAGTG
4141 ctcacccacgcgaaaaccttcctcaggaccctggtccgaggtgtccctgagtatggctgc 4200
GAGTGGGTGCGCTTTTGGAAGGAGTCCTGGGACCAGGCTCCACAGGGACTCATACCGACG
4201 gtggtgaacttgcggaagacagtggtgaacttccctgtagaagacgaggccctgggtggc 4260
CACCACTTGAACGCCTTCTGTCACCACTTGAAGGGACATCTTCTGCTCCGGGACCCACCG
4261 acggcttttgttcagatgccggcccacggcctattcccctggtgcggcctgctgctggat 4320
TGCCGAAAACAAGTCTACGGCCGGGTGCCGGATAAGGGGACCACGCCGGACGACGACCTA
4321 acccggaccctggaggtgcagagcgactactccagctatgcccggacctccatcagagcc 4380
TGGGCCTGGGACCTCCACGTCTCGCTGATGAGGTCGATACGGGCCTGGAGGTAGTCTCGG
4381 agtctcaccttcaaccgcggcttcaaggctgggaggaacatgcgtcgcaaactctttggg 4440
TCAGAGTGGAAGTTGGCGCCGAAGTTCCGACCCTCCTTGTACGCAGCGTTTGAGAAACCC
4441 gtcttgcggctgaagtgtcacagcctgtttctggatttgcaggtgaacagcctccagacg 4500
CAGAACGCCGACTTCACAGTGTCGGACAAAGACCTAAACGTCCACTTGTCGGAGGTCTGC
4501 gtgtgcaccaacatctacaagatcctcctgctgcaggcgtacaggtttcacgcatgtgtg 4560
CACACGTGGTTGTAGATGTTCTAGGAGGACGACGTCCGCATGTCCAAAGTGCGTACACAC
4561 ctgcagctcccatttcatcagcaagtttggaagaaccccacatttttcctgcgcgtcatc 4620
GACGTCGAGGGTAAAGTAGTCGTTCAAACCTTCTTGGGGTGTAAAAAGGACGCGCAGTAG
4621 tctgacacggcctccctctgctactccatcctgaaagccaagaacgcagggatgtcgctg 4680
AGACTGTGCCGGAGGGAGACGATGAGGTAGGACTTTCGGTTCTTGCGTCCCTACAGCGAC
4681 ggggccaagggcgccgccggccctctgccctccgaggccgtgcagtggctgtgccaccaa 4740
CCCCGGTTCCCGCGGCGGCCGGGAGACGGGAGGCTCCGGCACGTCACCGACACGGTGGTT
4741 gcattcctgctcaagctgactcgacaccgtgtcacctacgtgccactcctggggtcactc 4800
CGTAAGGACGAGTTCGACTGAGCTGTGGCACAGTGGATGCACGGTGAGGACCCCAGTGAG
4801 aggacagcccagacgcagctgagtcggaagctcccggggacgacgctgactgccctggag 4860
TCCTGTCGGGTCTGCGTCGACTCAGCCTTCGAGGGCCCCTGCTGCGACTGACGGGACCTC
4861 gccgcagccaacccggcactgccctcagacttcaagaccatcctggactgatggccaccc 4920
CGGCGTCGGTTGGGCCGTGACGGGAGTCTGAAGTTCTGGTAGGACCTGACTACCGGTGGG
4921 gcccacagccaggccgagagcagacaccagcagccctgtcacgccgggctctacgtccca 4980
CGGGTGTCGGTCCGGCTCTCGTCTGTGGTCGTCGGGACAGTGCGGCCCGAGATGCAGGGT
4981 gggagggaggggcggcccacacccaggcccgcaccgctgggagtctgaggcctgagtgag 5040
CCCTCCCTCCCCGCCGGGTGTGGGTCCGGGCGTGGCGACCCTCAGACTCCGGACTCACTC
5041 tgtttggccgaggcctgcatgtccggctgaaggctgagtgtccggctgaggcctgagcga 5100
ACAAACCGGCTCCGGACGTACAGGCCGACTTCCGACTCACAGGCCGACTCCGGACTCGCT
5101 gtgtccagccaagggctgagtgtccagcacacctgccgtcttcacttccccacaggctgg 5160
CACAGGTCGGTTCCCGACTCACAGGTCGTGTGGACGGCAGAAGTGAAGGGGTGTCCGACC
NotI XbaI
| |
5161 cgctcggctccaccctgcagatatccagcacagtggcggccgctcgagtctagagggccc 5220
GCGAGCCGAGGTGGGACGTCTATAGGTCGTGTCACCGCCGGCGAGCTCAGATCTCCCGGG
5221 gtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcc 5280
CAAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGG
5281 cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaa 5340
GGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTT
5341 atgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtgg 5400
TACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACC
5401 ggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgg 5460
CCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCCACC
ClaI
|
5461 gctctatggcttctgaggcggaaagaaccagcagatcgatctgcttatgggtattatggg 5520
CGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGAATACCCATAATACCC
5521 tctgcattaatgaatcggccaacggatcccggtgtgaaataccgcacagatgcgtaagga 5580
AGACGTAATTACTTAGCCGGTTGCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCT
5581 gaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcg 5640
CTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGC
Kan/Neo(5661,6441)>>>
|
5641 ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaat 5700
AAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTA
5701 caggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgta 5760
GTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCAT
pBR322 origin(5768,6387)<<<
|
5761 aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa 5820
TTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTT
5821 atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc 5880
TAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAG
5881 cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgt 5940
GGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACA
5941 ccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca 6000
GGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGT
6001 gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg 6060
CAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGC
6061 accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat 6120
TGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATA
6121 cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta 6180
GCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGAT
6181 cagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct 6240
GTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGA
6241 gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaac 6300
CGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTG
6301 aaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaa 6360
TTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTT
6361 aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaa 6420
TTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTT
6421 actcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttt 6480
TGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAA
6481 taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgaca 6540
ATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGT
6541 gttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca 6600
CAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGT
6601 tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcc 6660
ATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGG
6661 ccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataa 6720
GGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATT
6721 accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc 6780
TGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGG
6781 agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgca 6840
TCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGT
6841 acgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaa 6900
TGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTT
6901 acggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaag 6960
TGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTC
6961 cgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggt 7020
GCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCA
NEOKAN prom(7040,7089)>>>
|
7021 tttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaa 7080
AAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTT
7081 gccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatc 7140
CGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAG
NTP_II marker(7181,7969)>>>
|
ORF_2 rf(2)(7178,7972)>>>
| |
7141 aagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgca 7200
TTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGT
7201 cgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagac 7260
GCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTG
7261 aatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttt 7320
TTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAA
7321 tgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatc 7380
ACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAG
7381 gtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggg 7440
CACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCC
7441 aagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgc 7500
TTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACG
7501 tcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatcc 7560
AGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGG
7561 ggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggat 7620
CCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTA
7621 ggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagc 7680
CCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCG
7681 cgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgaccca 7740
GCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGT
7741 tggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcga 7800
ACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCT
7801 ctgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatat 7860
GACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATA
7861 tgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgc 7920
ACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCG
7921 tcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtt 7980
AGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAA
7981 aaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctt 8040
TTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAA
f1 origin(8077,8382)<<<
|
8041 ataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtc 8100
TATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAG
8101 cactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatg 8160
GTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTAC
8161 gcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctc 8220
CGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAG
8221 taaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacg 8280
ATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGC
8281 tggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag 8340
ACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATC
8341 cggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtc 8400
GCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAG
8401 cattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 8456
GTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
Misc. Feature (4 total)
CMV promoter
Start: 626 End: 1366
Original Location Description:
626..1366
hTERT
Start: 1457 End: 5170
ORI
Start: 5662 End: 6441
Original Location Description:
1942..2721
Kan
Start: 7187 End: 7978
Original Location Description:
3467..4258