pAAV-siRNA-GFP

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggta 661 cgtccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac 721 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag 781 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 841 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 901 tatatatctt gtggaaagga caaaaatagt cttctctaga gatatcattt aaatgtcgac 961 gaattcctcg agagaagaca gctttttttg gtgtttcgtg gtctcataca gaacttataa 1021 gattcccaaa tccaaagaca tttcacgttt atggtgattt cccagaacac atagcgacat 1081 gcaaatattg cagggcgcca ctcccctgtc cctcacagcc atcttcctgc cagggcgcac 1141 gcgcgctggg tgttcccgcc tagtgacact gggcccgcga ttccttggag cgggttgatg 1201 acgtcagcgt tctcaattgt agtaatcaat tacggggtca ttagttcata gcccatatat 1261 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 1321 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 1381 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 1441 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 1501 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 1561 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 1621 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 1681 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 1741 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagggtac 1801 cggatccctt aagtgaacaa ctagtgccac catggtgagc aagggcgagg agctgttcac 1861 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 1921 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 1981 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 2041 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 2101 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2161 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2221 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 2281 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 2341 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 2401 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 2461 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 2521 cactctcggc atggacgagc tgtacaagga attcggaagc gggcagtgca ccaactacgc 2581 cctgctgaag ctggccggcg acgtggagag caaccccggc cccggatcca tggccaccga 2641 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 2701 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 2761 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 2821 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 2881 agcgggggcg gtgttcgccg agatcggctc gcgcatggcc gagttgagcg gttcccggct 2941 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 3001 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 3061 cgtgctcccc ggagtggagg cggccgagcg cgctggggtg cccgccttcc tggagacctc 3121 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 3181 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtct 3241 taaggcgatc gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat 3301 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 3361 tataagctgc aataaacaag ttaataacaa caattccatt cattttatgt ttcaggttca 3421 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtagtcgaaa 3481 ttcccgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc 3541 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 3601 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 3661 gtgagcgagc gagcgcgcag ggatcccggt gtgaaatacc gcacagatgc gtaaggagaa 3721 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 3781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 4201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 4261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4321 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 4381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 4561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 4621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 4681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 4741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 4801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 4861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 4921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 4981 ttgttgaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg 5041 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc 5101 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt 5161 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc 5221 ctgcaaagta aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag 5281 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 5341 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 5401 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 5461 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 5521 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 5581 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5641 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5701 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5761 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5821 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 5881 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5941 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6001 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6061 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa 6121 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaac atcccttata 6181 aatcaaaaga atagaccgcg atagggttga gtgttgttcc agtttggaac aagagtccac 6241 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 6301 cactacgtga accatcaccc aaatcaagtt ttttgcggtc gaggtgccgt aaagctctaa 6361 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 6421 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 6481 tcacgctgcg cgtaaccacc acacccgcgc gcttaatgcg ccgctacagg gcgcgtccat 6541 tcgccattca ggatcgaatt aattcttaat taacatcatc aataatatac ctt

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggta 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCAT hU6 prom(666,921)>>> | 661 cgtccaaggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatac 720 GCAGGTTCCAGCCCGTCCTTCTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATG 721 gatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattag 780 CTATGTTCCGACAATCTCTCTATTAATCTTAATTAAACTGACATTTGTGTTTCTATAATC 781 tacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattat 840 ATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCATCAAACGTCAAAATTTTAATA 841 gttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctt 900 CAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAA XbaI EcoRV | | 901 tatatatcttgtggaaaggacaaaaatagtcttctctagagatatcatttaaatgtcgac 960 ATATATAGAACACCTTTCCTGTTTTTATCAGAAGAGATCTCTATAGTAAATTTACAGCTG XhoI | 961 gaattcctcgagagaagacagctttttttggtgtttcgtggtctcatacagaacttataa 1020 CTTAAGGAGCTCTCTTCTGTCGAAAAAAACCACAAAGCACCAGAGTATGTCTTGAATATT 1021 gattcccaaatccaaagacatttcacgtttatggtgatttcccagaacacatagcgacat 1080 CTAAGGGTTTAGGTTTCTGTAAAGTGCAAATACCACTAAAGGGTCTTGTGTATCGCTGTA 1081 gcaaatattgcagggcgccactcccctgtccctcacagccatcttcctgccagggcgcac 1140 CGTTTATAACGTCCCGCGGTGAGGGGACAGGGAGTGTCGGTAGAAGGACGGTCCCGCGTG ApaI | 1141 gcgcgctgggtgttcccgcctagtgacactgggcccgcgattccttggagcgggttgatg 1200 CGCGCGACCCACAAGGGCGGATCACTGTGACCCGGGCGCTAAGGAACCTCGCCCAACTAC 1201 acgtcagcgttctcaattgtagtaatcaattacggggtcattagttcatagcccatatat 1260 TGCAGTCGCAAGAGTTAACATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATA 1261 ggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgaccc 1320 CCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGG 1321 ccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttcca 1380 GGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGT 1381 ttgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgta 1440 AACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACAT 1441 tcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcatta 1500 AGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAAT 1501 tgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcat 1560 ACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTA 1561 cgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttga 1620 GCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACT 1621 ctcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcacca 1680 GAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGT 1681 aaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcgg 1740 TTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCC 1741 taggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagggtac 1800 ATCCGCACATGCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCCCATG ORF_1 rf(2)(1832,3232)>>> | KpnI EGFP reporter(1832,2548)>>> | | 1801 cggatcccttaagtgaacaactagtgccaccatggtgagcaagggcgaggagctgttcac 1860 GCCTAGGGAATTCACTTGTTGATCACGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTG 1861 cggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgt 1920 GCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCA 1921 gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcac 1980 CAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTG 1981 caccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgca 2040 GTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGT 2041 gtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcc 2100 CACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGG 2101 cgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccg 2160 GCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGC 2161 cgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcga 2220 GCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCT 2221 cttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaa 2280 GAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTT 2281 cgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcca 2340 GCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGT 2341 caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcgg 2400 GTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCC 2401 cgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaa 2460 GCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTT 2461 agaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggat 2520 TCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTA 2521 cactctcggcatggacgagctgtacaaggaattcggaagcgggcagtgcaccaactacgc 2580 GTGAGAGCCGTACCTGCTCGACATGTTCCTTAAGCCTTCGCCCGTCACGTGGTTGATGCG puro marker(2636,3232)>>> | 2581 cctgctgaagctggccggcgacgtggagagcaaccccggccccggatccatggccaccga 2640 GGACGACTTCGACCGGCCGCTGCACCTCTCGTTGGGGCCGGGGCCTAGGTACCGGTGGCT 2641 gtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccct 2700 CATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGA 2701 cgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacat 2760 GCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTA 2761 cgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaa 2820 GCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTT 2821 ggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcga 2880 CCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCT 2881 agcgggggcggtgttcgccgagatcggctcgcgcatggccgagttgagcggttcccggct 2940 TCGCCCCCGCCACAAGCGGCTCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGA StuI | 2941 ggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtg 3000 CCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCAC 3001 gttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgt 3060 CAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCA 3061 cgtgctccccggagtggaggcggccgagcgcgctggggtgcccgccttcctggagacctc 3120 GCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAG 3121 cgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcga 3180 GCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCT 3181 ggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgtct 3240 CCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAGA 3241 taaggcgatcgcagacatgataagatacattgatgagtttggacaaaccacaactagaat 3300 ATTCCGCTAGCGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTA 3301 gcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccat 3360 CGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTA 3361 tataagctgcaataaacaagttaataacaacaattccattcattttatgtttcaggttca 3420 ATATTCGACGTTATTTGTTCAATTATTGTTGTTAAGGTAAGTAAAATACAAAGTCCAAGT 3421 gggggagatgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtagtcgaaa 3480 CCCCCTCTACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATCAGCTTT 3481 ttcccgataaggatcttcctagagcatggctacgtagataagtagcatggcgggttaatc 3540 AAGGGCTATTCCTAGAAGGATCTCGTACCGATGCATCTATTCATCGTACCGCCCAATTAG 3541 attaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcg 3600 TAATTGATGTTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGC 3601 ctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctca 3660 GAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGT 3661 gtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaaggagaa 3720 CACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTT 3721 aataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttc 3780 TTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAG 3781 ggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcag 3840 CCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTC 3841 gggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaa 3900 CCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTT pBR322 origin(3905,4524)<<< | 3901 aggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc 3960 TCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAG 3961 gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccc 4020 CTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGG 4021 ctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccg 4080 GACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGC 4081 cctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtt 4140 GGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAA 4141 cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgacc 4200 GCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGG 4201 gctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgc 4260 CGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCG 4261 cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacag 4320 GTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTC 4321 agttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcg 4380 TCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGC 4381 ctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaa 4440 GAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTT 4441 ccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaag 4500 GGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTC 4501 gatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact 4560 CTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGA 4561 cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa 4620 GTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATT 4621 attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagtt 4680 TAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAA 4681 accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatag 4740 TGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATC 4741 ttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccca 4800 AACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGT 4801 gtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacc 4860 CACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGG 4861 agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagt 4920 TCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCA 4921 ctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacg 4980 GATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGC 4981 ttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacg 5040 AACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGC 5041 gtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgc 5100 CACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCG 5101 aaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggtttt 5160 TTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAA NEOKAN prom(5177,5226)>>> | 5161 atggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagcc 5220 TACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGG 5221 ctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaag 5280 GACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTC NTP_II marker(5318,6106)>>> | BclI ORF_2 rf(2)(5315,6109)>>> | | | 5281 ctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgc 5340 GAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCG 5341 aggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaat 5400 TCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTA 5401 cggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgt 5460 GCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACA 5461 caagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtg 5520 GTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCAC 5521 gctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaag 5580 CGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTC 5581 ggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcc 5640 CCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGG 5641 tgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggc 5700 ACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCG 5701 tacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgga 5760 ATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCT 5761 agccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccga 5820 TCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCT SphI | 5821 actgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatgg 5880 TGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACC 5881 cgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactg 5940 GCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGAC 5941 tggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgc 6000 ACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACG 6001 tgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcc 6060 ACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGG 6061 cgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaa 6120 GCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTT 6121 atttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttata 6180 TAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATAT f1 origin(6214,6519)<<< | 6181 aatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccac 6240 TTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTG 6241 tattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcc 6300 ATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGG 6301 cactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaa 6360 GTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATT 6361 atcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtgg 6420 TAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACC 6421 cgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcgg 6480 GCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCC 6481 tcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccat 6540 AGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTA 6541 tcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 6593 AGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA