pAAV-PGK-saCas9
| Vector Name | pAAV-PGK-saCas9 |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggtc
661 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
721 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
781 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
841 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
901 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
961 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
1021 gttctcctct tcctcatctc cgggcctttc gacgaattcg ccaccatggc cccaaagaag
1081 aagcggaagg tcggtatcca cggagtccca gcagccaagc ggaactacat cctgggcctg
1141 gacatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg ggacgtgatc
1201 gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg caggcggagc
1261 aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag agtgaagaag
1321 ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat caacccctac
1381 gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc tgccgccctg
1441 ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc
1501 aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga agagaaatac
1561 gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg cagcatcaac
1621 agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc
1681 taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga aacccggcgg
1741 acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat caaagaatgg
1801 tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag cgtgaagtac
1861 gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt gatcaccagg
1921 gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa cgtgttcaag
1981 cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat
2041 attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct gaaggtgtac
2101 cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga gctgctggat
2161 cagattgcca agatcctgac catctaccag agcagcgagg acatccagga agaactgacc
2221 aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct gaagggctat
2281 accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga gctgtggcac
2341 accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa gaaggtggac
2401 ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct gagccccgtc
2461 gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc
2521 ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga cgcccagaaa
2581 atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga ggaaatcatc
2641 cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct gcacgacatg
2701 caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct gctgaacaac
2761 cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga caacagcttc
2821 aacaacaagg tgctcgtgaa gcaggaagaa aacagcaaga agggcaaccg gaccccattc
2881 cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa gcacatcctg
2941 aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct gctggaagaa
3001 cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct ggtggatacc
3061 agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt gaacaacctg
3121 gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg gaagtggaag
3181 tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct gatcattgcc
3241 aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa agtgatggaa
3301 aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac cgagcaggag
3361 tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt caaggactac
3421 aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga caccctgtac
3481 tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa cggcctgtac
3541 gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg
3601 taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca gtacggcgac
3661 gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac caagtactcc
3721 aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa actgaacgcc
3781 catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg
3841 aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt gaccgtgaag
3901 aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg ctatgaggaa
3961 gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt ctacaacaac
4021 gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg
4081 aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga aaacatgaac
4141 gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag cattaagaag
4201 tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca ccctcagatc
4261 atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
4321 ggatcctgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
4381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
4441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
4501 gggaggattg ggaagagaat agcaggcatg ctggggacgt agataagtag catggcgggt
4561 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4621 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4681 cctcagtgag cgagcgagcg cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag
4741 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
5401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
6001 caacgttgtt gaaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag
6061 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
6121 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
6181 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
6241 aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg gcgcagggga
6301 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
6361 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
6421 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
6481 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
6541 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
6601 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
6661 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
6721 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
6781 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
6841 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
6901 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
6961 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
7021 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
7081 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg
7141 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaacatccc
7201 ttataaatca aaagaataga ccgcgatagg gttgagtgtt gttccagttt ggaacaagag
7261 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
7321 tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt gccgtaaagc
7381 tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
7441 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
7501 agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta atgcgccgct acagggcgcg
7561 tccattcgcc attcaggatc gaattaattc ttaattaaca tcatcaataa tatacctt
LOCUS dna 7618 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
ORF 832..4329
/sequence="ORF_1 rf(1)"
ORF 997..3783
/sequence="ORF_3 rf(5)"
misc_binding 1054..1059
/dbxref="REBASE:EcoRI"
misc_binding 1198..1203
/dbxref="REBASE:ClaI"
misc_binding 1570..1575
/dbxref="REBASE:PstI"
misc_binding 2469..2474
/dbxref="REBASE:HindIII"
Rep_Origin 4930..5549
/gene="pBR322 origin"
Promoter 6202..6251
/gene="NEOKAN prom"
ORF 6340..7134
/sequence="ORF_2 rf(1)"
Marker 6343..7131
/gene="NTP_II marker"
Rep_Origin 7239..7544
/gene="f1 origin"
BASE COUNT 2053 a 2000 c 2116 g 1449 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggtc
661 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
721 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
781 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
841 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
901 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
961 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
1021 gttctcctct tcctcatctc cgggcctttc gacgaattcg ccaccatggc cccaaagaag
1081 aagcggaagg tcggtatcca cggagtccca gcagccaagc ggaactacat cctgggcctg
1141 gacatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg ggacgtgatc
1201 gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg caggcggagc
1261 aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag agtgaagaag
1321 ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat caacccctac
1381 gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc tgccgccctg
1441 ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc
1501 aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga agagaaatac
1561 gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg cagcatcaac
1621 agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc
1681 taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga aacccggcgg
1741 acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat caaagaatgg
1801 tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag cgtgaagtac
1861 gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt gatcaccagg
1921 gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa cgtgttcaag
1981 cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat
2041 attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct gaaggtgtac
2101 cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga gctgctggat
2161 cagattgcca agatcctgac catctaccag agcagcgagg acatccagga agaactgacc
2221 aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct gaagggctat
2281 accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga gctgtggcac
2341 accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa gaaggtggac
2401 ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct gagccccgtc
2461 gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc
2521 ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga cgcccagaaa
2581 atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga ggaaatcatc
2641 cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct gcacgacatg
2701 caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct gctgaacaac
2761 cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga caacagcttc
2821 aacaacaagg tgctcgtgaa gcaggaagaa aacagcaaga agggcaaccg gaccccattc
2881 cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa gcacatcctg
2941 aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct gctggaagaa
3001 cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct ggtggatacc
3061 agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt gaacaacctg
3121 gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg gaagtggaag
3181 tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct gatcattgcc
3241 aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa agtgatggaa
3301 aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac cgagcaggag
3361 tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt caaggactac
3421 aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga caccctgtac
3481 tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa cggcctgtac
3541 gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg
3601 taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca gtacggcgac
3661 gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac caagtactcc
3721 aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa actgaacgcc
3781 catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg
3841 aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt gaccgtgaag
3901 aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg ctatgaggaa
3961 gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt ctacaacaac
4021 gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg
4081 aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga aaacatgaac
4141 gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag cattaagaag
4201 tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca ccctcagatc
4261 atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
4321 ggatcctgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
4381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
4441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
4501 gggaggattg ggaagagaat agcaggcatg ctggggacgt agataagtag catggcgggt
4561 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4621 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4681 cctcagtgag cgagcgagcg cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag
4741 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
5401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
6001 caacgttgtt gaaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag
6061 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
6121 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
6181 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
6241 aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg gcgcagggga
6301 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
6361 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
6421 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
6481 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
6541 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
6601 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
6661 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
6721 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
6781 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
6841 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
6901 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
6961 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
7021 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
7081 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg
7141 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaacatccc
7201 ttataaatca aaagaataga ccgcgatagg gttgagtgtt gttccagttt ggaacaagag
7261 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
7321 tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt gccgtaaagc
7381 tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
7441 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
7501 agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta atgcgccgct acagggcgcg
7561 tccattcgcc attcaggatc gaattaattc ttaattaaca tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggtc 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCAG
661 caccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcc 720
GTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAGG
721 tcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaat 780
AGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTA
ORF_1 rf(1)(832,4329)>>>
|
781 ggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagcaatggaagcg 840
CCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGTCGTGGCGACTCGTTACCTTCGC
841 ggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagag 900
CCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCGAGTCTC
901 gctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgc 960
CGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCG
ORF_3 rf(5)(997,3783)<<<
|
961 ccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct 1020
GGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAGACGGCGCGA
EcoRI
|
1021 gttctcctcttcctcatctccgggcctttcgacgaattcgccaccatggccccaaagaag 1080
CAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCTGCTTAAGCGGTGGTACCGGGGTTTCTTC
1081 aagcggaaggtcggtatccacggagtcccagcagccaagcggaactacatcctgggcctg 1140
TTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGTTCGCCTTGATGTAGGACCCGGAC
1141 gacatcggcatcaccagcgtgggctacggcatcatcgactacgagacacgggacgtgatc 1200
CTGTAGCCGTAGTGGTCGCACCCGATGCCGTAGTAGCTGATGCTCTGTGCCCTGCACTAG
ClaI
|
1201 gatgccggcgtgcggctgttcaaagaggccaacgtggaaaacaacgagggcaggcggagc 1260
CTACGGCCGCACGCCGACAAGTTTCTCCGGTTGCACCTTTTGTTGCTCCCGTCCGCCTCG
1261 aagagaggcgccagaaggctgaagcggcggaggcggcatagaatccagagagtgaagaag 1320
TTCTCTCCGCGGTCTTCCGACTTCGCCGCCTCCGCCGTATCTTAGGTCTCTCACTTCTTC
1321 ctgctgttcgactacaacctgctgaccgaccacagcgagctgagcggcatcaacccctac 1380
GACGACAAGCTGATGTTGGACGACTGGCTGGTGTCGCTCGACTCGCCGTAGTTGGGGATG
1381 gaggccagagtgaagggcctgagccagaagctgagcgaggaagagttctctgccgccctg 1440
CTCCGGTCTCACTTCCCGGACTCGGTCTTCGACTCGCTCCTTCTCAAGAGACGGCGGGAC
1441 ctgcacctggccaagagaagaggcgtgcacaacgtgaacgaggtggaagaggacaccggc 1500
GACGTGGACCGGTTCTCTTCTCCGCACGTGTTGCACTTGCTCCACCTTCTCCTGTGGCCG
1501 aacgagctgtccaccaaagagcagatcagccggaacagcaaggccctggaagagaaatac 1560
TTGCTCGACAGGTGGTTTCTCGTCTAGTCGGCCTTGTCGTTCCGGGACCTTCTCTTTATG
PstI
|
1561 gtggccgaactgcagctggaacggctgaagaaagacggcgaagtgcggggcagcatcaac 1620
CACCGGCTTGACGTCGACCTTGCCGACTTCTTTCTGCCGCTTCACGCCCCGTCGTAGTTG
1621 agattcaagaccagcgactacgtgaaagaagccaaacagctgctgaaggtgcagaaggcc 1680
TCTAAGTTCTGGTCGCTGATGCACTTTCTTCGGTTTGTCGACGACTTCCACGTCTTCCGG
1681 taccaccagctggaccagagcttcatcgacacctacatcgacctgctggaaacccggcgg 1740
ATGGTGGTCGACCTGGTCTCGAAGTAGCTGTGGATGTAGCTGGACGACCTTTGGGCCGCC
1741 acctactatgagggacctggcgagggcagccccttcggctggaaggacatcaaagaatgg 1800
TGGATGATACTCCCTGGACCGCTCCCGTCGGGGAAGCCGACCTTCCTGTAGTTTCTTACC
1801 tacgagatgctgatgggccactgcacctacttccccgaggaactgcggagcgtgaagtac 1860
ATGCTCTACGACTACCCGGTGACGTGGATGAAGGGGCTCCTTGACGCCTCGCACTTCATG
1861 gcctacaacgccgacctgtacaacgccctgaacgacctgaacaatctcgtgatcaccagg 1920
CGGATGTTGCGGCTGGACATGTTGCGGGACTTGCTGGACTTGTTAGAGCACTAGTGGTCC
1921 gacgagaacgagaagctggaatattacgagaagttccagatcatcgagaacgtgttcaag 1980
CTGCTCTTGCTCTTCGACCTTATAATGCTCTTCAAGGTCTAGTAGCTCTTGCACAAGTTC
1981 cagaagaagaagcccaccctgaagcagatcgccaaagaaatcctcgtgaacgaagaggat 2040
GTCTTCTTCTTCGGGTGGGACTTCGTCTAGCGGTTTCTTTAGGAGCACTTGCTTCTCCTA
2041 attaagggctacagagtgaccagcaccggcaagcccgagttcaccaacctgaaggtgtac 2100
TAATTCCCGATGTCTCACTGGTCGTGGCCGTTCGGGCTCAAGTGGTTGGACTTCCACATG
2101 cacgacatcaaggacattaccgcccggaaagagattattgagaacgccgagctgctggat 2160
GTGCTGTAGTTCCTGTAATGGCGGGCCTTTCTCTAATAACTCTTGCGGCTCGACGACCTA
2161 cagattgccaagatcctgaccatctaccagagcagcgaggacatccaggaagaactgacc 2220
GTCTAACGGTTCTAGGACTGGTAGATGGTCTCGTCGCTCCTGTAGGTCCTTCTTGACTGG
2221 aatctgaactccgagctgacccaggaagagatcgagcagatctctaatctgaagggctat 2280
TTAGACTTGAGGCTCGACTGGGTCCTTCTCTAGCTCGTCTAGAGATTAGACTTCCCGATA
2281 accggcacccacaacctgagcctgaaggccatcaacctgatcctggacgagctgtggcac 2340
TGGCCGTGGGTGTTGGACTCGGACTTCCGGTAGTTGGACTAGGACCTGCTCGACACCGTG
2341 accaacgacaaccagatcgctatcttcaaccggctgaagctggtgcccaagaaggtggac 2400
TGGTTGCTGTTGGTCTAGCGATAGAAGTTGGCCGACTTCGACCACGGGTTCTTCCACCTG
2401 ctgtcccagcagaaagagatccccaccaccctggtggacgacttcatcctgagccccgtc 2460
GACAGGGTCGTCTTTCTCTAGGGGTGGTGGGACCACCTGCTGAAGTAGGACTCGGGGCAG
HindIII
|
2461 gtgaagagaagcttcatccagagcatcaaagtgatcaacgccatcatcaagaagtacggc 2520
CACTTCTCTTCGAAGTAGGTCTCGTAGTTTCACTAGTTGCGGTAGTAGTTCTTCATGCCG
2521 ctgcccaacgacatcattatcgagctggcccgcgagaagaactccaaggacgcccagaaa 2580
GACGGGTTGCTGTAGTAATAGCTCGACCGGGCGCTCTTCTTGAGGTTCCTGCGGGTCTTT
2581 atgatcaacgagatgcagaagcggaaccggcagaccaacgagcggatcgaggaaatcatc 2640
TACTAGTTGCTCTACGTCTTCGCCTTGGCCGTCTGGTTGCTCGCCTAGCTCCTTTAGTAG
2641 cggaccaccggcaaagagaacgccaagtacctgatcgagaagatcaagctgcacgacatg 2700
GCCTGGTGGCCGTTTCTCTTGCGGTTCATGGACTAGCTCTTCTAGTTCGACGTGCTGTAC
2701 caggaaggcaagtgcctgtacagcctggaagccatccctctggaagatctgctgaacaac 2760
GTCCTTCCGTTCACGGACATGTCGGACCTTCGGTAGGGAGACCTTCTAGACGACTTGTTG
2761 cccttcaactatgaggtggaccacatcatccccagaagcgtgtccttcgacaacagcttc 2820
GGGAAGTTGATACTCCACCTGGTGTAGTAGGGGTCTTCGCACAGGAAGCTGTTGTCGAAG
2821 aacaacaaggtgctcgtgaagcaggaagaaaacagcaagaagggcaaccggaccccattc 2880
TTGTTGTTCCACGAGCACTTCGTCCTTCTTTTGTCGTTCTTCCCGTTGGCCTGGGGTAAG
2881 cagtacctgagcagcagcgacagcaagatcagctacgaaaccttcaagaagcacatcctg 2940
GTCATGGACTCGTCGTCGCTGTCGTTCTAGTCGATGCTTTGGAAGTTCTTCGTGTAGGAC
2941 aatctggccaagggcaagggcagaatcagcaagaccaagaaagagtatctgctggaagaa 3000
TTAGACCGGTTCCCGTTCCCGTCTTAGTCGTTCTGGTTCTTTCTCATAGACGACCTTCTT
3001 cgggacatcaacaggttctccgtgcagaaagacttcatcaaccggaacctggtggatacc 3060
GCCCTGTAGTTGTCCAAGAGGCACGTCTTTCTGAAGTAGTTGGCCTTGGACCACCTATGG
3061 agatacgccaccagaggcctgatgaacctgctgcggagctacttcagagtgaacaacctg 3120
TCTATGCGGTGGTCTCCGGACTACTTGGACGACGCCTCGATGAAGTCTCACTTGTTGGAC
3121 gacgtgaaagtgaagtccatcaatggcggcttcaccagctttctgcggcggaagtggaag 3180
CTGCACTTTCACTTCAGGTAGTTACCGCCGAAGTGGTCGAAAGACGCCGCCTTCACCTTC
3181 tttaagaaagagcggaacaaggggtacaagcaccacgccgaggacgccctgatcattgcc 3240
AAATTCTTTCTCGCCTTGTTCCCCATGTTCGTGGTGCGGCTCCTGCGGGACTAGTAACGG
3241 aacgccgatttcatcttcaaagagtggaagaaactggacaaggccaaaaaagtgatggaa 3300
TTGCGGCTAAAGTAGAAGTTTCTCACCTTCTTTGACCTGTTCCGGTTTTTTCACTACCTT
3301 aaccagatgttcgaggaaaagcaggccgagagcatgcccgagatcgaaaccgagcaggag 3360
TTGGTCTACAAGCTCCTTTTCGTCCGGCTCTCGTACGGGCTCTAGCTTTGGCTCGTCCTC
3361 tacaaagagatcttcatcaccccccaccagatcaagcacattaaggacttcaaggactac 3420
ATGTTTCTCTAGAAGTAGTGGGGGGTGGTCTAGTTCGTGTAATTCCTGAAGTTCCTGATG
3421 aagtacagccaccgggtggacaagaagcctaatagagagctgattaacgacaccctgtac 3480
TTCATGTCGGTGGCCCACCTGTTCTTCGGATTATCTCTCGACTAATTGCTGTGGGACATG
3481 tccacccggaaggacgacaagggcaacaccctgatcgtgaacaatctgaacggcctgtac 3540
AGGTGGGCCTTCCTGCTGTTCCCGTTGTGGGACTAGCACTTGTTAGACTTGCCGGACATG
3541 gacaaggacaatgacaagctgaaaaagctgatcaacaagagccccgaaaagctgctgatg 3600
CTGTTCCTGTTACTGTTCGACTTTTTCGACTAGTTGTTCTCGGGGCTTTTCGACGACTAC
3601 taccaccacgacccccagacctaccagaaactgaagctgattatggaacagtacggcgac 3660
ATGGTGGTGCTGGGGGTCTGGATGGTCTTTGACTTCGACTAATACCTTGTCATGCCGCTG
3661 gagaagaatcccctgtacaagtactacgaggaaaccgggaactacctgaccaagtactcc 3720
CTCTTCTTAGGGGACATGTTCATGATGCTCCTTTGGCCCTTGATGGACTGGTTCATGAGG
3721 aaaaaggacaacggccccgtgatcaagaagattaagtattacggcaacaaactgaacgcc 3780
TTTTTCCTGTTGCCGGGGCACTAGTTCTTCTAATTCATAATGCCGTTGTTTGACTTGCGG
3781 catctggacatcaccgacgactaccccaacagcagaaacaaggtcgtgaagctgtccctg 3840
GTAGACCTGTAGTGGCTGCTGATGGGGTTGTCGTCTTTGTTCCAGCACTTCGACAGGGAC
3841 aagccctacagattcgacgtgtacctggacaatggcgtgtacaagttcgtgaccgtgaag 3900
TTCGGGATGTCTAAGCTGCACATGGACCTGTTACCGCACATGTTCAAGCACTGGCACTTC
3901 aatctggatgtgatcaaaaaagaaaactactacgaagtgaatagcaagtgctatgaggaa 3960
TTAGACCTACACTAGTTTTTTCTTTTGATGATGCTTCACTTATCGTTCACGATACTCCTT
3961 gctaagaagctgaagaagatcagcaaccaggccgagtttatcgcctccttctacaacaac 4020
CGATTCTTCGACTTCTTCTAGTCGTTGGTCCGGCTCAAATAGCGGAGGAAGATGTTGTTG
4021 gatctgatcaagatcaacggcgagctgtatagagtgatcggcgtgaacaacgacctgctg 4080
CTAGACTAGTTCTAGTTGCCGCTCGACATATCTCACTAGCCGCACTTGTTGCTGGACGAC
4081 aaccggatcgaagtgaacatgatcgacatcacctaccgcgagtacctggaaaacatgaac 4140
TTGGCCTAGCTTCACTTGTACTAGCTGTAGTGGATGGCGCTCATGGACCTTTTGTACTTG
4141 gacaagaggccccccaggatcattaagacaatcgcctccaagacccagagcattaagaag 4200
CTGTTCTCCGGGGGGTCCTAGTAATTCTGTTAGCGGAGGTTCTGGGTCTCGTAATTCTTC
4201 tacagcacagacattctgggcaacctgtatgaagtgaaatctaagaagcaccctcagatc 4260
ATGTCGTGTCTGTAAGACCCGTTGGACATACTTCACTTTAGATTCTTCGTGGGAGTCTAG
4261 atcaaaaagggcaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag 4320
TAGTTTTTCCCGTTTTCCGGCCGCCGGTGCTTTTTCCGGCCGGTCCGTTTTTTCTTTTTC
4321 ggatcctgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgcctt 4380
CCTAGGACTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAA
4381 ccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcat 4440
GGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTA
4441 cgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagg 4500
GCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCC
4501 gggaggattgggaagagaatagcaggcatgctggggacgtagataagtagcatggcgggt 4560
CCCTCCTAACCCTTCTCTTATCGTCCGTACGACCCCTGCATCTATTCATCGTACCGCCCA
4561 taatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctc 4620
ATTAGTAATTGATGTTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAG
4621 gctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcgg 4680
CGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCC
4681 cctcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaag 4740
GGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTC
4741 gagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt 4800
CTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCA
4801 cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacaga 4860
GCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCT
4861 atcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg 4920
TAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGC
pBR322 origin(4930,5549)<<<
|
4921 taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa 4980
ATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTT
4981 aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtt 5040
TTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAA
5041 tccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct 5100
AGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGA
5101 gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatct 5160
CAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGA
5161 cagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcc 5220
GTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGG
5221 cgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgactt 5280
GCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAA
5281 atcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgc 5340
TAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACG
5341 tacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtat 5400
ATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATA
5401 ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaa 5460
GACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTT
5461 acaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaa 5520
TGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTT
5521 aaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacga 5580
TTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCT
5581 aaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcct 5640
TTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGA
5641 tttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctga 5700
AAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACT
5701 cagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatc 5760
GTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAG
5761 catagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctgg 5820
GTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACC
5821 ccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaat 5880
GGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTA
5881 aaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccat 5940
TTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTA
5941 ccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcg 6000
GGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGC
6001 caacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcag 6060
GTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTC
6061 aaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgca 6120
TTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGT
6121 agcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcg 6180
TCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGC
NEOKAN prom(6202,6251)>>>
|
6181 gttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttggg 6240
CAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCC
6241 aagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcagggga 6300
TTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCT
NTP_II marker(6343,7131)>>>
|
ORF_2 rf(1)(6340,7134)>>>
| |
6301 tcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattg 6360
AGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAAC
6361 cacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacag 6420
GTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTC
6421 acaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctt 6480
TGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAA
6481 tttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggcta 6540
AAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGAT
6541 tcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcg 6600
AGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGC
6601 ggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacctt 6660
CCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAA
6661 gctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgat 6720
CGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTA
6721 ccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcgg 6780
GGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCC
6781 atggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcca 6840
TACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGT
6841 gccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacc 6900
CGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGG
6901 catggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatc 6960
GTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAG
6961 gactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgat 7020
CTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTA
7021 attgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgcc 7080
TAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGG
7081 gctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttg 7140
CGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAAC
7141 ttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccc 7200
AATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGG
f1 origin(7239,7544)<<<
|
7201 ttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagag 7260
AATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTC
7261 tccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcga 7320
AGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCT
7321 tggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagc 7380
ACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCG
7381 tctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaa 7440
AGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTT
7441 cgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgt 7500
GCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACA
7501 agcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcg 7560
TCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGC
7561 tccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 7618
AGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 7618 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
ORF 832..4329
/sequence="ORF_1 rf(1)"
ORF 997..3783
/sequence="ORF_3 rf(5)"
misc_binding 1054..1059
/dbxref="REBASE:EcoRI"
misc_binding 1198..1203
/dbxref="REBASE:ClaI"
misc_binding 1570..1575
/dbxref="REBASE:PstI"
misc_binding 2469..2474
/dbxref="REBASE:HindIII"
Rep_Origin 4930..5549
/gene="pBR322 origin"
Promoter 6202..6251
/gene="NEOKAN prom"
ORF 6340..7134
/sequence="ORF_2 rf(1)"
Marker 6343..7131
/gene="NTP_II marker"
Rep_Origin 7239..7544
/gene="f1 origin"
BASE COUNT 2053 a 2000 c 2116 g 1449 t 0 others